The ectomycorrhizal fungal communities associated with Quercus liaotungensis in different habitats across northern China.

Quercus liaotungensis is a major tree species in deciduous broad-leaved forests in northern China. In this study, we investigated ectomycorrhizal (ECM) communities associated with Q. liaotungensis from five typical habitats across northern China. We used internal transcribed spacer-polymerase chain reaction and DNA sequencing to identify ECM fungi, and we detected 220 operational taxonomic units. In general, at the regional scale, the dominant ECM lineages were /tomentella-thelephora, /cenococcum, /russula-lactarius, and /inocybe. Analysis of variance demonstrated significant differences in alpha diversity among these ECM communities, and the ECM fungal richness was positively correlated with elevation and soil organic matter. Analysis of similarity and a nonmetric multidimensional scaling analysis revealed that there were significant differences in community composition, and the geographical distance was correlated with the ECM fungal communities. Among the environmental factors we studied, soil parameters and climate factors were the primary direct driving factors of the ECM fungal communities. Our study primarily advances our understanding of environmental factors affecting ECM fungal communities at regional scale.

Diversity and community structure of ectomycorrhizal fungi associated with Larix chinensis across the alpine treeline ecotone of Taibai Mountain.

Alpine treeline ecotones represent ecosystems that are vulnerable to climate change. We investigated the ectomycorrhizal (ECM) community, which has potential to stabilize alpine ecosystems. ECM communities associated with Larix chinensis were studied in four zones along a natural ecotone from a mixed forest stand over pure forest stands, the timberline, and eventually, the treeline (3050-3450 m) in Tabai Mountain, China. Sixty operational taxonomic units (OTUs) of ECM fungi were identified by sequencing the rDNA internal transcribed spacer of ECM tips. The richness of ECM species increased with elevation. The soil C/N ratio was the most important factor explaining ECM species richness. The treeline zone harbored some unique ECM fungi whereas no unique genera were observed in the timberline and pure forest zone. Elevation and topography were equally important factors influencing ECM communities in the alpine region. We suggest that a higher diversity of the ECM fungal community associated with L. chinensis in the treeline zone could result from niche differentiation.

Ectomycorrhizal fungal communities associated with Populus simonii and Pinus tabuliformis in the hilly-gully region of the Loess Plateau, China.

The Loess Plateau region of northwestern China has unique geological and dry/semi-dry climate characteristics. However, knowledge about ectomycorrhizal fungal (EMF) communities in the Loess Plateau is limited. In this study, we investigated EMF communities in Populus simonii and Pinus tabuliformis patches within the forest-steppe zone, in pine forests within the forest zone, and the transitional zone between them. We revealed high species richness (115 operational taxonomic units [OTUs]) of indigenous EMF resources at the Loess Plateau, of which Tomentella (35 OTUs), Inocybe (16), Sebacina (16), and Geopora (7) were the most OTU-rich lineages. EMF richness within the forest-steppe zone and the transitional zone was limited, while the natural pine forest maintained diverse EMF communities in the forest zone. The changes of EMF community richness and composition along arid eco-zones were highlighted for the complex factors including precipitation, soil factors, host, DBH, and altitude. Indicator analysis revealed that some EMF showed clear host preference and some taxa, i.e., genera Geopora and Inocybe, were dominant in drought and alkaline-saline conditions attributed to their environmental preference. This study revealed that EMF communities were quite limited in the forest-steppe zone, while the forest region contained diverse EMF communities in the Loess Plateau.