RESUMEN
In this work, we present the Genome Modeling System (GMS), an analysis information management system capable of executing automated genome analysis pipelines at a massive scale. The GMS framework provides detailed tracking of samples and data coupled with reliable and repeatable analysis pipelines. The GMS also serves as a platform for bioinformatics development, allowing a large team to collaborate on data analysis, or an individual researcher to leverage the work of others effectively within its data management system. Rather than separating ad-hoc analysis from rigorous, reproducible pipelines, the GMS promotes systematic integration between the two. As a demonstration of the GMS, we performed an integrated analysis of whole genome, exome and transcriptome sequencing data from a breast cancer cell line (HCC1395) and matched lymphoblastoid line (HCC1395BL). These data are available for users to test the software, complete tutorials and develop novel GMS pipeline configurations. The GMS is available at https://github.com/genome/gms.
Asunto(s)
Mapeo Cromosómico/métodos , Genoma Humano/genética , Bases del Conocimiento , Modelos Genéticos , Análisis de Secuencia de ADN/métodos , Interfaz Usuario-Computador , Algoritmos , Simulación por Computador , Sistemas de Administración de Bases de Datos , Bases de Datos Genéticas , Humanos , Alineación de Secuencia/métodosRESUMEN
The objective of this article is to review knowledge on the hookworm Uncinaria lucasi Stiles, 1901 in northern fur seals, Callorhinus ursinus Linnaeus, 1758. Emphasis is placed on research on this host-parasite system in the Pribilof Islands, AK, USA where the bulk of the studies has been performed.
Asunto(s)
Ancylostomatoidea/patogenicidad , Lobos Marinos/parasitología , Animales , Investigación Biomédica/tendencias , Interacciones Huésped-ParásitosRESUMEN
An experimental investigation of electron impact excitation of the 2p state of atomic hydrogen is described. A beam of electrons was passed through a chopped beam of hydrogen atoms in a high vacuum apparatus. The modulated flux of Lyman-α photons emitted in the radiative decay of the 2p state was taken as a measurement of the excitation probability resulting from direct excitation plus indirect excitation resulting from cascading. The region surrounding the intersection of the two beams was electrically and magnetically shielded to prevent quenching of metastable 2s atoms and thereby to ensure that the observed Lyman-α flux resulted from decay of the short-lived 2p state. The experimental results are consistent with those obtained by Fite, Stebbings, and Brackmann [1959], and confirm the existence of a large discrepancy between theoretical and experimental results in the electron energy range below 50 eV.