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1.
Chemistry ; 30(37): e202400468, 2024 Jul 02.
Artículo en Inglés | MEDLINE | ID: mdl-38683640

RESUMEN

Two series of metallo-(Zn(II), Mg(II), and Ru(II)) and free-base phthalocyanines (Pcs) with a carboxyl anchoring group and well-established bulky peripheral substituents (either tert-butyl or bulky 2,6-diisopropylphenoxy) were synthesized and tested as sensitizers in dye-sensitized solar cells (DSSCs). The trend of photovoltaic efficiencies (PCEs) for free-base and metallo Pcs followed the order Zn(II)Pc>Mg(II)Pc≫H2Pc ≈ Ru(II)Pc regardless of the peripheral substitution. Higher efficiencies (4.95 versus 3.63 for the Zn(II) derivatives) were achieved with Pcs bearing the bulkier 2,6-diisopropylphenoxy group, indicating a lower aggregation and more suitable HOMO-LUMO levels. Furthermore, these derivatives showed a morelevant influence of the metal on the PCE values (from the highest 4.95 for the Zn(II)Pc to the lowest 0.23 for the Ru(II)Pc. In both series, the best PCEs observed with the Zn(II) derivatives were mainly due to their highest Jsc values. The lowest efficiencies found for the free-bases and Ru(II) derivatives were attributed to a mismatch between their LUMO levels and the conduction band of the TiO2,and lower light-harvesting capabilities, respectively. In conclusion, Zn(II) derivatives are still the best Pc candidates to use as sensitizers in molecular photovoltaics.

2.
Chem Soc Rev ; 51(16): 6965-7045, 2022 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-35686606

RESUMEN

The increasing energy demand and environmental issues caused by the over-exploitation of fossil fuels render the need for renewable, clean, and environmentally benign energy sources unquestionably urgent. The zero-emission energy carrier, H2 is an ideal alternative to carbon-based fuels especially when it is generated photocatalytically from water. Additionally, the photocatalytic conversion of CO2 into chemical fuels can reduce the CO2 emissions and have a positive environmental and economic impact. Inspired by natural photosynthesis, plenty of artificial photocatalytic schemes based on porphyrinoids have been investigated. This review covers the recent advances in photocatalytic H2 production and CO2 reduction systems containing porphyrin or phthalocyanine derivatives. The unique properties of porphyrinoids enable their utilization both as chromophores and as catalysts. The homogeneous photocatalytic systems are initially described, presenting the various approaches for the improvement of photosensitizing activity and the enhancement of catalytic performance at the molecular level. On the other hand, for the development of the heterogeneous systems, numerous methods were employed such as self-assembled supramolecular porphyrinoid nanostructures, construction of organic frameworks, combination with 2D materials and adsorption onto semiconductors. The dye sensitization on semiconductors opened the way for molecular-based dye-sensitized photoelectrochemical cells (DSPECs) devices based on porphyrins and phthalocyanines. The research in photocatalytic systems as discussed herein remains challenging since there are still many limitations making them unfeasible to be used at a large scale application before finding a large-scale application.


Asunto(s)
Porfirinas , Biomimética , Dióxido de Carbono/química , Isoindoles , Fotosíntesis , Porfirinas/química
3.
Phys Chem Chem Phys ; 23(43): 24545-24549, 2021 Nov 10.
Artículo en Inglés | MEDLINE | ID: mdl-34704576

RESUMEN

We have studied the suitability of using a molecular rotor-based steady-state fluorometric assay for evaluating changes in both the conformation and the viscosity of collagen-like peptide solutions. Our results indicate that a positive charge incorporated on the hydrophobic tail of the BODIPY molecular rotor favours the dye specificity as a reporter for viscosity of these solutions.


Asunto(s)
Péptidos/química , Compuestos de Boro/química , Colágeno/química , Colorantes Fluorescentes/química , Interacciones Hidrofóbicas e Hidrofílicas , Conformación Molecular , Soluciones , Espectrometría de Fluorescencia , Viscosidad
4.
Biophys J ; 116(10): 1984-1993, 2019 05 21.
Artículo en Inglés | MEDLINE | ID: mdl-31053255

RESUMEN

Gravity-sensitive cellular responses are regularly observed in both specialized and nonspecialized cells. One potential mechanism for this sensitivity is a changing viscosity of the intracellular organelles. Here, we report a novel, to our knowledge, viscosity-sensitive molecular rotor based on mesosubstituted boron-dipyrrin used to investigate the response of viscosity of cellular membranes to hypergravity conditions created at the large diameter centrifuge at the European Space Agency Technology Centre. Mouse osteoblastic (MC3T3-E1) and endothelial (human umbilical vein endothelial cell) cell lines were tested, and an increase in viscosity was found with increasing hypergravity loading. This response is thought to be primarily biologically driven, with the potential for a small, instantaneous physical mechanism also contributing to the observed effect. This work provides the first, to our knowledge, quantitative data for cellular viscosity changes under hypergravity, up to 15 × g.


Asunto(s)
Gravitación , Espacio Intracelular/metabolismo , Células 3T3 , Animales , Fenómenos Biomecánicos , Compuestos de Boro/metabolismo , Células Endoteliales de la Vena Umbilical Humana/citología , Humanos , Ratones , Viscosidad
5.
Soft Matter ; 14(46): 9466-9474, 2018 Nov 28.
Artículo en Inglés | MEDLINE | ID: mdl-30427370

RESUMEN

Amyloid deposits of aggregated beta-amyloid Aß(1-42) peptides are a pathological hallmark of Alzheimer's disease. Aß(1-42) aggregates are known to induce biophysical alterations in cells, including disruption of plasma membranes. We investigated the microviscosity of plasma membranes upon interaction with oligomeric and fibrillar forms of Aß(1-42). Viscosity-sensing fluorophores termed molecular rotors were utilised to directly measure the microviscosities of giant plasma membrane vesicles (GPMVs) and plasma membranes of live SH-SY5Y and HeLa cells. The fluorescence lifetimes of membrane-inserting BODIPY-based molecular rotors revealed a decrease in bilayer microviscosity upon incubation with Aß(1-42) oligomers, while fibrillar Aß(1-42) did not significantly affect the microviscosity of the bilayer. In addition, we demonstrate that the neuroprotective peptide H3 counteracts the microviscosity change induced by Aß(1-42) oligomers, suggesting the utility of H3 as a neuroprotective therapeutic agent in neurodegenerative disorders and indicating that ligand-induced membrane stabilisation may be a possible mechanism of neuroprotection during neurodegenerative disorders such as Alzheimer's disease.


Asunto(s)
Péptidos beta-Amiloides/farmacología , Compuestos de Boro/farmacología , Membrana Celular/efectos de los fármacos , Colorantes Fluorescentes/farmacología , Fragmentos de Péptidos/farmacología , Línea Celular Tumoral , Membrana Celular/fisiología , Humanos , Neuropéptidos/farmacología , Viscosidad
6.
Phys Chem Chem Phys ; 19(37): 25252-25259, 2017 Sep 27.
Artículo en Inglés | MEDLINE | ID: mdl-28718466

RESUMEN

Microviscosity is a key parameter controlling the rate of diffusion and reactions on the microscale. One of the most convenient tools for measuring microviscosity is by fluorescent viscosity sensors termed 'molecular rotors'. BODIPY-based molecular rotors in particular proved extremely useful in combination with fluorescence lifetime imaging microscopy, for providing quantitative viscosity maps of living cells as well as measuring dynamic changes in viscosity over time. In this work, we investigate several new BODIPY-based molecular rotors with the aim of improving on the current viscosity sensing capabilities and understanding how the structure of the fluorophore is related to its function. We demonstrate that due to subtle structural changes, BODIPY-based molecular rotors may become sensitive to temperature and polarity of their environment, as well as to viscosity, and provide a photophysical model explaining the nature of this sensitivity. Our data suggests that a thorough understanding of the photophysics of any new molecular rotor, in environments of different viscosity, temperature and polarity, is a must before moving on to applications in viscosity sensing.

7.
Org Biomol Chem ; 13(12): 3792-802, 2015 Mar 28.
Artículo en Inglés | MEDLINE | ID: mdl-25703541

RESUMEN

We report the synthesis of four new cationic dipolar push­pull dyes, together with an evaluation of their photophysical and photobiological characteristics pertinent to imaging membranes by fluorescence and second harmonic generation (SHG). All four dyes consist of an N,N-diethylaniline electron-donor conjugated to a pyridinium electron-acceptor via a thiophene bridge, with either vinylene (­CH=CH­) or ethynylene (­C≡C­) linking groups, and with either singly-charged or doubly-charged pyridinium terminals. The absorption and fluorescence behavior of these dyes were compared to a commercially available fluorescent membrane stain, the styryl dye FM4-64. The hyperpolarizabilities of all dyes were compared using hyper-Rayleigh scattering at 800 nm. Cellular uptake, localization, toxicity and phototoxicity were evaluated using tissue cell cultures (HeLa, SK-OV-3 and MDA-231). Replacing the central alkene bridge of FM4-64 with a thiophene does not substantially change the absorption, fluorescence or hyperpolarizability, whereas changing the vinylene-links to ethynylenes shifts the absorption and fluorescence to shorter wavelengths, and reduces the hyperpolarizability by about a factor of two. SHG and fluorescence imaging experiments in live cells showed that the doubly-charged thiophene dyes localize in plasma membranes, and exhibit lower internalization rates compared to FM4-64, resulting in less signal from the cell cytosol. At a typical imaging concentration of 1 µM, the doubly-charged dyes showed no significant light or dark toxicity, whereas the singly-charged dyes are phototoxic even at 0.5 µM. The doubly-charged dyes showed phototoxicity at concentrations greater than 10 µM, although they do not generate singlet oxygen, indicating that the phototoxicity is type I rather than type II. The doubly-charged thiophene dyes are more effective than FM4-64 as SHG dyes for live cells.


Asunto(s)
Membrana Celular/química , Colorantes/química , Tiofenos/química , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Humanos , Modelos Moleculares , Dinámicas no Lineales , Fenómenos Ópticos , Espectrometría de Fluorescencia , Electricidad Estática , Liposomas Unilamelares/química
8.
Phys Chem Chem Phys ; 17(28): 18393-402, 2015 Jul 28.
Artículo en Inglés | MEDLINE | ID: mdl-26104504

RESUMEN

In order to fully understand the dynamics of processes within biological lipid membranes, it is necessary to possess an intimate knowledge of the physical state and ordering of lipids within the membrane. Here we report the use of three molecular rotors based on meso-substituted boron-dipyrrin (BODIPY) in combination with fluorescence lifetime spectroscopy to investigate the viscosity and phase behaviour of model lipid bilayers. In phase-separated giant unilamellar vesicles, we visualise both liquid-ordered (Lo) and liquid-disordered (Ld) phases using fluorescence lifetime imaging microscopy (FLIM), determining their associated viscosity values, and investigate the effect of composition on the viscosity of these phases. Additionally, we use molecular dynamics simulations to investigate the orientation of the BODIPY probes within the bilayer, as well as using molecular dynamics simulations and fluorescence correlation spectroscopy (FCS) to compare diffusion coefficients with those predicted from the fluorescence lifetimes of the probes.


Asunto(s)
Compuestos de Boro/química , Membrana Dobles de Lípidos/química , Difusión , Simulación de Dinámica Molecular , Espectrometría de Fluorescencia , Liposomas Unilamelares/química , Viscosidad
9.
Commun Chem ; 6(1): 15, 2023 Jan 17.
Artículo en Inglés | MEDLINE | ID: mdl-36697756

RESUMEN

Lipid peroxidation is a process which is key in cell signaling and disease, it is exploited in cancer therapy in the form of photodynamic therapy. The appearance of hydrophilic moieties within the bilayer's hydrocarbon core will dramatically alter the structure and mechanical behavior of membranes. Here, we combine viscosity sensitive fluorophores, advanced microscopy, and X-ray diffraction and molecular simulations to directly and quantitatively measure the bilayer's structural and viscoelastic properties, and correlate these with atomistic molecular modelling. Our results indicate an increase in microviscosity and a decrease in the bending rigidity upon peroxidation of the membranes, contrary to the trend observed with non-oxidized lipids. Fluorescence lifetime imaging microscopy and MD simulations give evidence for the presence of membrane regions of different local order in the oxidized membranes. We hypothesize that oxidation promotes stronger lipid-lipid interactions, which lead to an increase in the lateral heterogeneity within the bilayer and the creation of lipid clusters of higher order.

10.
Sci Adv ; 8(14): eabm2094, 2022 Apr 08.
Artículo en Inglés | MEDLINE | ID: mdl-35394846

RESUMEN

Misfolding of secretory proteins in the endoplasmic reticulum (ER) features in many human diseases. In α1-antitrypsin deficiency, the pathogenic Z variant aberrantly assembles into polymers in the hepatocyte ER, leading to cirrhosis. We show that α1-antitrypsin polymers undergo a liquid:solid phase transition, forming a protein matrix that retards mobility of ER proteins by size-dependent molecular filtration. The Z-α1-antitrypsin phase transition is promoted during ER stress by an ATF6-mediated unfolded protein response. Furthermore, the ER chaperone calreticulin promotes Z-α1-antitrypsin solidification and increases protein matrix stiffness. Single-particle tracking reveals that solidification initiates in cells with normal ER morphology, previously assumed to represent a healthy pool. We show that Z-α1-antitrypsin-induced hypersensitivity to ER stress can be explained by immobilization of ER chaperones within the polymer matrix. This previously unidentified mechanism of ER dysfunction provides a template for understanding a diverse group of related proteinopathies and identifies ER chaperones as potential therapeutic targets.

11.
Chemistry ; 17(6): 1855-62, 2011 Feb 07.
Artículo en Inglés | MEDLINE | ID: mdl-21274937

RESUMEN

The synthesis of stopcocks based on zinc phthalocyanine for selective adsorption at the channel entrances of zeolite L is reported. The introduction of either an inert SiMe3 moiety, an imidazolium cation or a reactive isothiocyanate (NCS) group allows attachment to the channel entrances of zeolite L through van der Waals interactions, electrostatic interactions, or covalent binding, respectively. Stopcocks that rely on van-der-Waals-driven adsorption require careful selection of the solvent used for the deposition onto the zeolite surface to avoid a nonspecific distribution of the molecules. Regarding the design of photonic antenna systems, a stopcock with a cationic tail was found to be the most convenient, based on the observation that efficient energy transfer from molecules located in the zeolite nanochannels is more readily obtained than in the other cases.

12.
J Photochem Photobiol B ; 225: 112346, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34736070

RESUMEN

An eye lens is constantly exposed to the solar UV radiation, which is considered the most important external source of age-related changes to eye lens constituents. The accumulation of modifications of proteins and lipids with age can eventually lead to the development of progressive lens opacifications, such as cataracts. Though the impact of solar UV radiation on the structure and function of proteins is actively studied, little is known about the effect of photodamage on plasma membranes of lens cells. In this work we exploit Fluorescence Lifetime Imaging Microscopy (FLIM), together with viscosity-sensitive fluorophores termed molecular rotors, to study the changes in viscosity of plasma membranes of porcine eye lens resulting from two different types of photodamage: Type I (electron transfer) and Type II (singlet oxygen) reactions. We demonstrate that these two types of photodamage result in clearly distinct changes in viscosity - a decrease in the case of Type I damage and an increase in the case of Type II processes. Finally, to simulate age-related changes that occur in vivo, we expose an intact eye lens to UV-A light under anaerobic conditions. The observed decrease in viscosity within plasma membranes is consistent with the ability of eye lens constituents to sensitize Type I photodamage under natural irradiation conditions. These changes are likely to alter the transport of metabolites and predispose the whole tissue to the development of pathological processes such as cataracts.


Asunto(s)
Cristalino/efectos de la radiación , Rayos Ultravioleta , Animales , Membrana Celular/efectos de la radiación , Técnicas In Vitro , Oxígeno Singlete/metabolismo , Porcinos , Viscosidad
13.
Chem Sci ; 12(7): 2604-2613, 2020 Dec 22.
Artículo en Inglés | MEDLINE | ID: mdl-34164028

RESUMEN

Lipid packing in cellular membranes has a direct effect on membrane tension and microviscosity, and plays a central role in cellular adaptation, homeostasis and disease. According to conventional mechanical descriptions, viscosity and tension are directly interconnected, with increased tension leading to decreased membrane microviscosity. However, the intricate molecular interactions that combine to build the structure and function of a cell membrane suggest a more complex relationship between these parameters. In this work, a viscosity-sensitive fluorophore ('molecular rotor') is used to map changes in microviscosity in model membranes under conditions of osmotic stress. Our results suggest that the relationship between membrane tension and microviscosity is strongly influenced by the bilayer's lipid composition. In particular, we show that the effects of increasing tension are minimised for membranes that exhibit liquid disordered (Ld) - liquid ordered (Lo) phase coexistence; while, surprisingly, membranes in pure gel and Lo phases exhibit a negative compressibility behaviour, i.e. they soften upon compression.

14.
J Biomed Opt ; 25(12)2020 12.
Artículo en Inglés | MEDLINE | ID: mdl-33331150

RESUMEN

SIGNIFICANCE: Despite the importance of the cell membrane in regulation of drug activity, the influence of drug treatments on its physical properties is still poorly understood. The combination of fluorescence lifetime imaging microscopy (FLIM) with specific viscosity-sensitive fluorescent molecular rotors allows the quantification of membrane viscosity with high spatiotemporal resolution, down to the individual cell organelles. AIM: The aim of our work was to analyze microviscosity of the plasma membrane of living cancer cells during chemotherapy with cisplatin using FLIM and correlate the observed changes with lipid composition and cell's response to treatment. APPROACH: FLIM together with viscosity-sensitive boron dipyrromethene-based fluorescent molecular rotor was used to map the fluidity of the cell's membrane. Chemical analysis of membrane lipid composition was performed with time-of-flight secondary ion mass spectrometry (ToF-SIMS). RESULTS: We detected a significant steady increase in membrane viscosity in viable cancer cells, both in cell monolayers and tumor spheroids, upon prolonged treatment with cisplatin, as well as in cisplatin-adapted cell line. ToF-SIMS revealed correlative changes in lipid profile of cisplatin-treated cells. CONCLUSIONS: These results suggest an involvement of membrane viscosity in the cell adaptation to the drug and in the acquisition of drug resistance.


Asunto(s)
Cisplatino , Neoplasias , Cisplatino/farmacología , Colorantes Fluorescentes , Microscopía Fluorescente , Orgánulos , Viscosidad
15.
Sci Rep ; 10(1): 14063, 2020 08 20.
Artículo en Inglés | MEDLINE | ID: mdl-32820221

RESUMEN

Membrane fluidity plays an important role in many cell functions such as cell adhesion, and migration. In stem cell lines membrane fluidity may play a role in differentiation. Here we report the use of viscosity-sensitive fluorophores based on a BODIPY core, termed "molecular rotors", in combination with Fluorescence Lifetime Imaging Microscopy, for monitoring of plasma membrane viscosity changes in mesenchymal stem cells (MSCs) during osteogenic and chondrogenic differentiation. In order to correlate the viscosity values with membrane lipid composition, the detailed analysis of the corresponding membrane lipid composition of differentiated cells was performed by time-of-flight secondary ion mass spectrometry. Our results directly demonstrate for the first time that differentiation of MSCs results in distinct membrane viscosities, that reflect the change in lipidome of the cells following differentiation.


Asunto(s)
Compuestos de Boro/química , Diferenciación Celular , Colorantes Fluorescentes/química , Fluidez de la Membrana , Células Madre Mesenquimatosas/citología , Microscopía Fluorescente/métodos , Viscosidad , Antígenos CD/análisis , Membrana Celular , Células Cultivadas , Condrogénesis , Humanos , Osteogénesis , Espectrometría de Masa de Ion Secundario
16.
J Am Chem Soc ; 131(24): 8669-76, 2009 Jun 24.
Artículo en Inglés | MEDLINE | ID: mdl-19530734

RESUMEN

Within the present work, two series of novel ruthenium(II) phthalocyanine (RuPc) complexes with one [RuPcCOPy-nT] or two [RuPc(Py-nT)(2)] dendritic oligothiophene (DOT) ligands in the axial positions are reported. The ability of Ru(II) for axial coordination in RuPcs allowed the attachment of the Pc through the metal site to the DOT-ligands bearing pyridine at the core position of the dendrons. These extended pyridine functionalized conjugated DOT-ligands (Py-nT) were chosen to cover the spectral window between 380 and 550 nm, where the RuPc does not exhibit a strong absorption, in order to improve the light-absorption of these complexes and hence enhance the efficiency of the corresponding solar cells. Good efficiencies of up to 1.6% have been achieved when blended together with a fullerene acceptor in solution-processed photovoltaic devices, providing by far the best phthalocyanine-based bulk heterojunction solar cells reported to-date.

17.
ACS Appl Mater Interfaces ; 11(40): 36307-36315, 2019 Oct 09.
Artículo en Inglés | MEDLINE | ID: mdl-31513373

RESUMEN

Molecular mobility in neuronal plasma membranes is a crucial factor in brain function. Microscopic viscosity is an important parameter that determines molecular mobility. This study presents the first direct measurement of the microviscosity of plasma membranes of live neurons. Microviscosity maps were obtained using fluorescence lifetime imaging of environment-sensing dyes termed "molecular rotors". Neurons were investigated both in the basal state and following common neurodegenerative stimuli, excitotoxicity, or oxidative stress. Both types of neurotoxic challenges induced microviscosity decrease in cultured neurons, and oxidant-induced membrane fluidification was counteracted by the wide-spectrum neuroprotectant, the H3 peptide. These results provide new insights into molecular mobility in neuronal membranes, paramount for basic brain function, and suggest that preservation of membrane stability may be an important aspect of neuroprotection in brain insults and neurodegenerative disorders.


Asunto(s)
Membrana Celular/fisiología , Colorantes Fluorescentes/metabolismo , Neuronas/citología , Neuroprotección , Estrés Oxidativo , Animales , Compuestos de Boro/química , Membrana Celular/efectos de los fármacos , Colorantes Fluorescentes/química , Células HeLa , Humanos , Peróxido de Hidrógeno/toxicidad , Neuroprotección/efectos de los fármacos , Fármacos Neuroprotectores/farmacología , Fenómenos Ópticos , Estrés Oxidativo/efectos de los fármacos , Ratas , Viscosidad
18.
Chem Commun (Camb) ; (10): 1187-9, 2008 Mar 14.
Artículo en Inglés | MEDLINE | ID: mdl-18309412

RESUMEN

We report the first phthalocyanine-based stopcock for selective adsorption to the channel entrances of zeolite L and realisation of a new electronic dipole moment coupling situation.

19.
Methods Appl Fluoresc ; 6(3): 034001, 2018 Apr 13.
Artículo en Inglés | MEDLINE | ID: mdl-29611817

RESUMEN

Surface functionalisation with viscosity sensitive dyes termed 'molecular rotors' can potentially open up new opportunities in sensing, for example for non-invasive biological viscosity imaging, in studying the effect of shear stress on lipid membranes and in cells, and in imaging contacts between surfaces upon applied pressure. We have functionalised microscope slides with BODIPY-based molecular rotor capable of viscosity sensing via its fluorescence lifetime. We have optimised functionalisation conditions and prepared the slides with the BODIPY rotor attached directly to the surface of glass slides and through polymer linkers of 5 kDa and 40 kDa in mass. The slides were characterised for their sensitivity to viscosity, and used to measure viscosity of supported lipid bilayers during photooxidation, and of giant unilamellar vesicles lying on the surface of the slide. We conclude that our functionalised slides show promise for a variety of viscosity sensing applications.

20.
ACS Nano ; 12(5): 4398-4407, 2018 05 22.
Artículo en Inglés | MEDLINE | ID: mdl-29648785

RESUMEN

Microscopic viscosity (microviscosity) is a key determinant of diffusion in the cell and defines the rate of biological processes occurring at the nanoscale, including enzyme-driven metabolism and protein folding. Here we establish a rotor-based organelle viscosity imaging (ROVI) methodology that enables real-time quantitative mapping of cell microviscosity. This approach uses environment-sensitive dyes termed molecular rotors, covalently linked to genetically encoded probes to provide compartment-specific microviscosity measurements via fluorescence lifetime imaging. ROVI visualized spatial and temporal dynamics of microviscosity with suborganellar resolution, reporting on a microviscosity difference of nearly an order of magnitude between subcellular compartments. In the mitochondrial matrix, ROVI revealed several striking findings: a broad heterogeneity of microviscosity among individual mitochondria, unparalleled resilience to osmotic stress, and real-time changes in microviscosity during mitochondrial depolarization. These findings demonstrate the use of ROVI to explore the biophysical mechanisms underlying cell biological processes.


Asunto(s)
Compuestos de Boro/química , Colorantes Fluorescentes/química , Imagen Óptica , Orgánulos/química , Animales , Compuestos de Boro/síntesis química , Células COS , Células Cultivadas , Chlorocebus aethiops , Colorantes Fluorescentes/síntesis química , Ligandos , Modelos Moleculares , Viscosidad
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