Atomic resolution imaging using a novel, compact and stiff scanning tunnelling microscope in cryogen-free superconducting magnet.

We present the design and performance of a novel scanning tunnelling microscope (STM) operating in a cryogen-free superconducting magnet. Our home-built STM head is compact (51.5 mm long and 20 mm in diameter) and has a single arm that provides complete openness in the scanning area between the tip and sample. The STM head consists of two piezoelectric tubes (PTs), a piezoelectric scanning tube (PST) mounted on a well-polished zirconia shaft, and a large PT housed in a sapphire tube called the motor tube. The main body of the STM head is made of tantalum. In this design, we fixed the sapphire tube to the frame with screws so that the tube's position can be changed quickly. To analyse the stiffness of the STM head unit, we identified the lowest eigenfrequencies with 3 and 4 kHz in the bending modes, 8 kHz in a torsional mode, and 9 kHz in a longitudinal mode by finite element analysis, and also measured the low drift rates in the X-Y plane and in the Z direction. The high performance of the home-built STM was demonstrated by images of the hexagonal graphite lattice at 300 K and in a sweeping magnetic field from 0 T to 9 T. Our results confirm the high stability, vibration resistance, insensitivity to high magnetic fields and the application potential of our newly developed STM for the investigation of low-frequency systems with high static support stiffness in physics, chemistry, material and biological sciences.

Safety evaluation of mice exposed to 7.0-33.0 T high-static magnetic fields.

Magnetic resonance imaging (MRI) of 7 T and higher can provide superior image resolution and capability. Clinical tests have been performed in 9.4 T MRI, and 21.1 T small-bore-size MRI has also been tested in rodents. Although the safety issue is a prerequisite for their future medical application, there are very few relevant studies for the safety of static magnetic fields (SMFs) of ≧20 T. The aim of this study was to assess the biological effects of 7.0-33.0 T SMFs in healthy adult mice. This was a prospective study, in which 104 healthy adult C57BL/6 mice were divided into control, sham control, and 7.0-33.0 T SMF-exposed groups.The sham control group and SMF group were handled identically, except for the electric current for producing SMF. A separate control group was placed outside the magnet and their data were used as normal range. After 1 h exposure, all mice were routinely fed for another 2 months while their body weight and food/water consumption were monitored. After 2 months, their complete blood count, blood biochemistry, key organ weight, and histomorphology were examined. All data are normally distributed. Differences between the sham and SMF-exposed groups were evaluated by unpaired t test. Most indicators did not show statistically significant changes or were still within the normal ranges, with only a few exceptions. For example, mono % in Group 2 (11.1 T) is 6.03 ± 1.43% while the normal range is 6.60-9.90% (p < 0.05). The cholesterol level in 33 T group is 3.38 ± 0.36 mmol/L while the normal range is 2.48-3.29 mmol/L (p < 0.05). The high-density lipoprotein cholesterol level in 33 T group is 2.54 ± 0.29 mmol/L while the normal reference range is 1.89-2.43 mmol/L (p < 0.01). Exposure to 7.0-33.0 T for 1 h did not have detrimental effects on normal adult mice. LEVEL OF EVIDENCE: 1 TECHNICAL EFFICACY STAGE: 1.

Controllable Thickness Inhomogeneity and Berry Curvature Engineering of Anomalous Hall Effect in SrRuO3 Ultrathin Films.

In quantum matters hosting electron-electron correlation and spin-orbit coupling, spatial inhomogeneities, arising from competing ground states, can be essential for understanding exotic topological properties. A prominent example is Hall anomalies observed in SrRuO3 films, which were interpreted in terms of either magnetic skyrmion-induced topological Hall effect or inhomogeneous anomalous Hall effect (AHE). To clarify this ambiguity, we systematically investigated the evolution of AHE with controllable inhomogeneities in SrRuO3 film thickness (tSRO). By exploiting the step-flow growth of SrRuO3 films, we induced a microscopically ordered stripe pattern with one-unit-cell differences in tSRO. The associated spatial distribution of momentum-space Berry curvatures enables a two-channel AHE with hump-like Hall anomalies, which can be continuously engineered according to non-integer tSRO. We further microscopically characterized the stripe-like ferromagnetic domains and two-step magnetic switching behavior in the inhomogeneous SrRuO3 film. These unique features can be utilized to identify the two-channel AHE model and understand its microscopic origin.

Effects of 3.5-23.0 T static magnetic fields on mice: A safety study.

People are exposed to various magnetic fields, including the high static/steady magnetic field (SMF) of MRI, which has been increased to 9.4 T in preclinical investigations. However, relevant safety studies about high SMF are deficient. Here we examined whether 3.5-23.0 T SMF exposure for 2 h has severe long-term effects on mice using 112 C57BL/6J mice. The food/water consumption, blood glucose levels, blood routine, blood biochemistry, as well as organ weight and HE stains were all examined. The food consumption and body weight were slightly decreased for 23.0 T-exposed mice (14.6%, P < 0.01, and 1.75-5.57%, P < 0.05, respectively), but not the other groups. While total bilirubin (TBIL), white blood cells, platelet and lymphocyte numbers were affected by some magnetic conditions, most of them were still within normal reference range. Although 13.5 T magnetic fields with the highest gradient (117.2 T/m) caused spleen weight increase, the blood count and biochemistry results were still within the control reference range. Moreover, the highest field 23.0 T with no gradient did not cause organ weight or blood biochemistry abnormality, which indicates that field gradient is a key parameter. Collectively, these data suggest 3.5-23.0 T static magnetic field exposure for 2 h do not have severe long-term effects on mice.

Ferroelectrically tunable magnetic skyrmions in ultrathin oxide heterostructures.

Magnetic skyrmions are topologically protected whirling spin texture. Their nanoscale dimensions, topologically protected stability and solitonic nature, together are promising for future spintronics applications. To translate these compelling features into practical spintronic devices, a key challenge lies in achieving effective control of skyrmion properties, such as size, density and thermodynamic stability. Here, we report the discovery of ferroelectrically tunable skyrmions in ultrathin BaTiO3/SrRuO3 bilayer heterostructures. The ferroelectric proximity effect at the BaTiO3/SrRuO3 heterointerface triggers a sizeable Dzyaloshinskii-Moriya interaction, thus stabilizing robust skyrmions with diameters less than a hundred nanometres. Moreover, by manipulating the ferroelectric polarization of the BaTiO3 layer, we achieve local, switchable and nonvolatile control of both skyrmion density and thermodynamic stability. This ferroelectrically tunable skyrmion system can simultaneously enhance the integratability and addressability of skyrmion-based functional devices.

Flatbands and Emergent Ferromagnetic Ordering in Fe_{3}Sn_{2} Kagome Lattices.

A flatband representing a highly degenerate and dispersionless manifold state of electrons may offer unique opportunities for the emergence of exotic quantum phases. To date, definitive experimental demonstrations of flatbands remain to be accomplished in realistic materials. Here, we present the first experimental observation of a striking flatband near the Fermi level in the layered Fe_{3}Sn_{2} crystal consisting of two Fe kagome lattices separated by a Sn spacing layer. The band flatness is attributed to the local destructive interferences of Bloch wave functions within the kagome lattices, as confirmed through theoretical calculations and modelings. We also establish high-temperature ferromagnetic ordering in the system and interpret the observed collective phenomenon as a consequence of the synergetic effect of electron correlation and the peculiar lattice geometry. Specifically, local spin moments formed by intramolecular exchange interaction are ferromagnetically coupled through a unique network of the hexagonal units in the kagome lattice. Our findings have important implications to exploit emergent flat-band physics in special lattice geometries.

Cellular ATP levels are affected by moderate and strong static magnetic fields.

Mitochondrion is the major cellular energy producing organelle that is at the boundary between chemical reactions and physical processes. Although mitochondria have been shown to be affected by physical methods such as nonthermal plasma, whether static magnetic field (SMF) could also affect them is still unclear. Here we used rat adrenal PC12 cells to compare SMFs of different intensities for their effects on ATP (adenosine-5'-triphosphate), the major energy source produced by mitochondria, which is essential for various cellular processes. Our results show that although 0.26 or 0.50 T SMFs did not affect ATP, 1 T and 9 T SMFs affected ATP level differently and time-dependently. Moreover, SMF-induced ATP level fluctuations are correlated with mitochondrial membrane potential changes. Our study provides insights not only into understanding various cellular effects of SMFs, but also the potential clinical applications of SMFs. Bioelectromagnetics. 39:352-360, 2018. © 2018 Wiley Periodicals, Inc.

Quantum Percolation and Magnetic Nanodroplet States in Electronically Phase-Separated Manganite Nanowires.

One-dimensional (1D) confinement has been revealed to effectively tune the properties of materials in homogeneous states. The 1D physics can be further enriched by electronic inhomogeneity, which unfortunately remains largely unknown. Here we demonstrate the ultrahigh sensitivity to magnetic fluctuations and the tunability of phase stability in the electronic transport properties of self-assembled electronically phase-separated manganite nanowires with extreme aspect ratio. The onset of magnetic nanodroplet state, a precursor to the ferromagnetic metallic state, is unambiguously revealed, which is attributed to the small lateral size of the nanowires that is comparable to the droplet size. Moreover, the quasi-1D anisotropy stabilizes thin insulating domains to form intrinsic tunneling junctions in the low temperature range, which is robust even under magnetic field up to 14 T and thus essentially modifies the classic 1D percolation picture to stabilize a novel quantum percolation state. A new phase diagram is therefore established for the manganite system under quasi-1D confinement for the first time. Our findings offer new insight into understanding and manipulating the colorful properties of the electronically phase-separated systems via dimensionality engineering.

Comparison of Tibial Intramedullary Nailing Guided by Digital Technology Versus Conventional Method: A Prospective Study.

BACKGROUND This prospective study aimed to compare clinical effects of intramedullary nailing guided by digital and conventional technologies in treatment of tibial fractures. MATERIAL AND METHODS Thirty-two patients (mean age 43 years, 18 males and 14 females) who were treated for tibial fractures from October 2010 to October 2012 were enrolled. They were sequentially randomized to receive intramedullary nailing guided by either digital technology (digital group, n=16) or conventional technology (conventional group, n=16). The operation time, fluoroscopy times, fracture healing time, distance between the actual and planned insertion point, postoperative lower limb alignment, and functional recovery were recorded for all patients. RESULTS The mean operation time in the digital group was 43.1±6.2 min compared with 48.7±8.3 min for the conventional technology (P=0.039). The fluoroscopy times and distance between the actual and planned insertion point were significantly lower in the digital group than in the conventional group (both P<0.001). The accuracy rate of the insertion point was 99.12% by digital technology. No difference was found in fracture healing time and good postoperative lower limb alignment between the digital and conventional groups (P=0.083 and P=0.310), as well as the effective rate (100% vs. 87.50%, P=0.144). CONCLUSIONS Intramedullary nailing guided by digital technology has many advantages in treatment of tibial fractures compared to conventional technology, including shorter operation time, reduced fluoroscopy times, and decreased distance between the actual and planned insertion point of the intramedullary nail.

The coefficient of the voltage induced frequency shift measurement on a quartz tuning fork.

We have measured the coefficient of the voltage induced frequency shift (VIFS) of a 32.768 KHz quartz tuning fork. Three vibration modes were studied: one prong oscillating, two prongs oscillating in the same direction, and two prongs oscillating in opposite directions. They all showed a parabolic dependence of the eigen-frequency shift on the bias voltage applied across the fork, due to the voltage-induced internal stress, which varies as the fork oscillates. The average coefficient of the VIFS effect is as low as several hundred nano-Hz per millivolt, implying that fast-response voltage-controlled oscillators and phase-locked loops with nano-Hz resolution can be built.

Glovebox-assisted magnetic force microscope for studying air-sensitive samples in a cryogen-free magnet.

Most known two-dimensional magnets exhibit a high sensitivity to air, making direct characterization of their domain textures technically challenging. Herein, we report on the construction and performance of a glovebox-assisted magnetic force microscope (MFM) operating in a cryogen-free magnet, realizing imaging of the intrinsic magnetic structure of water and oxygen-sensitive materials. It features a compact tubular probe for a 50 mm-diameter variable temperature insert installed in a 12 T cryogen-free magnet. A detachable sealing chamber can be electrically connected to the tail of the probe, and its pump port can be opened and closed by a vacuum manipulator located on the top of the probe. This sealing chamber enables sample loading and positioning in the glove box and MFM transfer to the magnet maintained in an inert gas atmosphere (in this case, argon and helium gas). The performance of the MFM is demonstrated by directly imaging the surface (using no buffer layer, such as h-BN) of very air-sensitive van der Waals magnetic material chromium triiodide (CrI3) samples at low temperatures as low as 5 K and high magnetic fields up to 11.9 T. The system's adaptability permits replacing the MFM unit with a scanning tunneling microscope unit, enabling high-resolution atomic imaging of air-sensitive surface samples.

A novel STM for quality atomic resolution with piezoelectric motor of high compactness and simplicity.

Scanning tunneling microscope (STM) is a renowned scientific tool for obtaining high-resolution atomic images of materials. Herein, we present an innovative design of the scanning unit with a compact yet powerful inertial piezoelectric motor inspired by the Spider Drive motor principle. The scanning unit mainly consists of a small 9 mm long piezoelectric tube scanner (PTS), one end of which is coaxially connected to the main sapphire body of the STM. Of particular emphasis in this design is the piezoelectric shaft (PS), constructed from piezoelectric material instead of conventional metallic or zirconium materials. The PS is a rectangular piezoelectric stack composed of two piezoelectric plates, which are elastically clamped on the inner wall of the PTS via a spring strip. The PTS and PS expand and contract independently with each other to improve the inertial force and reduce the threshold voltage. To ensure the stability of the PS and balance the stepping performance of the inertial motor, a counterweight, and a matching conical spring are fixed at the tail of the PS. This innovative design allows for the assessment of scanning unit performance by applying a driving signal, threshold voltage is 50 V at room temperature. Step sizes vary from 0.1 to 1 µm by changing the driving signal at room temperature. Furthermore, we successfully obtained atomic-resolution images of a highly oriented pyrolytic graphite (HOPG) sample and low drift rates of 23.4 pm/min and 34.6 pm/min in X-Y plane and Z direction, respectively, under ambient conditions. This small, compact STM unit has the potential for the development of a rotatable STM for use in cryogen-free magnets, and superconducting magnets.

Isolated scan unit and scanning tunneling microscope for stable imaging in ultra-high magnetic fields.

The high resolution of a scanning tunneling microscope (STM) relies on the stability of its scan unit. In this study, we present an isolated scan unit featuring non-magnetic design and ultra-high stability, as well as bidirectional movement capability. Different types of piezoelectric motors can be incorporated into the scan unit to create a highly stable STM. The standalone structure of scan unit ensures a stable atomic imaging process by decreasing noise generated by motor. The non-magnetic design makes the scan unit work stable in high magnetic field conditions. Moreover, we have successfully constructed a novel STM based on the isolated scan unit, in which two inertial piezoelectric motors act as the coarse approach actuators. The exceptional performance of homebuilt STM is proved by the high-resolution atomic images and dI/dV spectrums on NbSe2 surface at varying temperatures, as well as the raw-data images of graphite obtained at ultra-high magnetic fields of 23 T. According to the literature research, no STM has previously reported the atomic image at extreme conditions of 2 K low temperature and 23 T ultra-high magnetic field. Additionally, we present the ultra-low drift rates between the tip and sample at varying temperatures, as well as when raising the magnetic fields from 0 T to 23 T, indicating the ultra-high stability of the STM in high magnetic field conditions. The outstanding performance of our stable STM hold great potential for investigating the materials in ultra-high magnetic fields.

An ultracompact scanning tunneling microscope within a Φ 10 piezo tube in a 20 T superconducting magnet.

Low-temperature scanning tunneling microscopy and spectroscopy (STM/S) help to better understand the fundamental physics of condensed matter. We present an ultracompact STM within a Φ 10 piezo tube in a 20 T superconducting magnet. The carefully cut piezo tube contains the STM's coarse-positioning assembly. Loading an STM tip-sample mechanical loop into the piezo tube with special cut openings enables an ultracompact pencil-size dimension down to Φ 10 mm, in which fine-machined nonmagnetic parts are assembled to enable slide-stick motion and xyz-scanning procedures. The small size leads to a higher resonant frequency, a typical feature of a rigid STM instrument, increasing its vibration immunity. Scanning by moving the sample while keeping the tip stationary improves the stability of the tip-sample junction compared to moving the tip. Taking advantage of its high-field compatibility and rigid design, our STM captures the atomically resolved topography of highly oriented pyrolytic graphite (HOPG) at 1.5 K and in magnetic fields up to 17 T. The topography of graphene lattice and graphite is simultaneously recorded on an atomic terrace of HOPG, unveiling a modified local charge density at a surface defect. The superconducting energy gaps of layered type-II superconductors NbSe2 and PdBi2 are well resolved through dI/dV tunneling spectra at sub-2 K. Our unique STM is highly suitable for potential STM/S applications in world-class high-field facilities where the strong magnetic field can exceed 30 T.

Atomic imaging with a 12 T magnetic field perpendicular or parallel to the sample surface by an ultra-stable scanning tunneling microscope.

We present the first nonmetallic scanning tunneling microscope (STM) featuring an ultra-stable tip-sample mechanical loop and capable of atomic-resolution imaging within a 12 T magnetic field that could be either perpendicular or parallel to the sample surface. This is also the first STM with an ultra-stable tip-sample mechanical loop but without a standalone scanner. The STM head is constructed only with two parts: an improved spider-drive motor and a zirconia tip holder. The motor performs both the coarse approach and atomic imaging. A supporting spring is set at the fixed end of the motor tube to decrease the tip-sample mechanical loop. The zirconia tip holder performs as the frame of the whole STM head. With the novel design, the STM head in three dimensions can be as small as 7.9 mm × 7.9 mm × 26.5 mm. The device's excellent performance is demonstrated by atomic-resolution images of graphite and NbSe2 obtained at 300 K and 2 K, as well as the high-resolution dI/dV spectrums of NbSe2 at variable temperatures. Low drift rates in the X-Y plane and Z direction further prove the imaging stability of our new STM. High-quality imaging of the Charge Density Wave (CDW) structure on a TaS2 surface shows the STM's good application capability. Continuous atomic images obtained in magnetic fields rangs from 0 T to 12 T with the direction of the magnetic field perpendicular or parallel to the sample surface show the STM's good immunity to high magnetic fields. Our results illustrate the new STM's broad application ability in extreme conditions of low temperature and high magnetic field.

Atomic-Resolution Imaging of Micron-Sized Samples Realized by High Magnetic Field Scanning Tunneling Microscopy.

Scanning tunneling microscopy (STM) can image material surfaces with atomic resolution, making it a useful tool in the areas of physics and materials. Many materials are synthesized at micron size, especially few-layer materials. Limited by their complex structure, very few STMs are capable of directly positioning and imaging a micron-sized sample with atomic resolution. Traditional STMs are designed to study the material behavior induced by temperature variation, while the physical properties induced by magnetic fields are rarely studied. In this paper, we present the design and construction of an atomic-resolution STM that can operate in a 9 T high magnetic field. More importantly, the homebuilt STM is capable of imaging micron-sized samples. The performance of the STM is demonstrated by high-quality atomic images obtained on a graphite surface, with low drift rates in the X-Y plane and Z direction. The atomic-resolution image obtained on a 32-µm graphite flake illustrates the new STM's ability of positioning and imaging micron-sized samples. Finally, we present atomic resolution images at a magnetic field range from 0 T to 9 T. The above advantages make our STM a promising tool for investigating the quantum hall effect of micron-sized layered materials.

Positioning and atomic imaging of micron-size graphene sheets by a scanning tunneling microscope.

We present a mechanism for directly positioning the tip over a micron-size sample by tracking the trajectory of the tip and tip shadow. A bilayer graphene sheet identified by Raman spectroscopy with a lateral size of 20 µm × 50 µm was transferred on the surface of shaped gold electrodes, on which it will be rapidly captured by a homebuilt scanning tunneling microscopy (STM) with the help of an optical microscope. Using the improved line-based imaging mode, atomic-resolution images featuring a hexagonal lattice structure on the bilayer graphene sheet were obtained by our positioning-capable STM. We have also observed a unique O-ring superstructure on graphene surface that caused by the collective interference near the boundaries or defects. Furthermore, we successfully captured a graphene sheet of size as small as 1.3 nm by a rapid and large-area searching operation; this is the first time that such a small graphene sheet has been observed with atomic resolution. The STM images of a micron-size graphene sheet illustrate the significant positioning ability and imaging precision of our homebuilt STM. Our results contribute to further STM studies on samples with ultra-small size.

A Novel Atomically Resolved Scanning Tunneling Microscope Capable of Working in Cryogen-Free Superconducting Magnet.

We present a novel homebuilt scanning tunneling microscope (STM) with atomic resolution integrated into a cryogen-free superconducting magnet system with a variable temperature insert. The STM head is designed as a nested structure of double piezoelectric tubes (PTs), which are connected coaxially through a sapphire frame whose top has a sample stage. A single shaft made of tantalum, with the STM tip on top, is held firmly by a spring strip inside the internal PT. The external PT drives the shaft to the tip-sample junction based on the SpiderDrive principle, and the internal PT completes the subsequent scanning and imaging work. The STM head is simple, compact, and easy to assemble. The excellent performance of the device was demonstrated by obtaining atomic-resolution images of graphite and low drift rates of 30.2 pm/min and 41.4 pm/min in the X-Y plane and Z direction, respectively, at 300K. In addition, we cooled the sample to 1.6 K and took atomic-resolution images of graphite and NbSe2. Finally, we performed a magnetic field sweep test from 0 T to 9 T at 70 K, obtaining distinct graphite images with atomic resolution under varying magnetic fields. These experiments show our newly developed STM's high stability, vibration resistance, and immunity to high magnetic fields.

A cryogen-free superconducting magnet based scanning tunneling microscope for liquid phase measurement.

Scanning tunneling microscopes (STMs) that work in ultra-high vacuum and low temperatures are commonly used in condensed matter physics, but an STM that works in a high magnetic field to image chemical molecules and active biomolecules in solution has never been reported. Here, we present a liquid-phase STM for use in a 10 T cryogen-free superconducting magnet. The STM head is mainly constructed with two piezoelectric tubes. A large piezoelectric tube is fixed at the bottom of a tantalum frame to perform large-area imaging. A small piezoelectric tube mounted at the free end of the large one performs high-precision imaging. The imaging area of the large piezoelectric tube is four times that of the small one. The high compactness and rigidity of the STM head make it functional in a cryogen-free superconducting magnet with huge vibrations. The performance of our homebuilt STM was demonstrated by the high-quality, atomic-resolution images of a graphite surface, as well as the low drift rates in the X-Y plane and Z direction. Furthermore, we successfully obtained atomic-resolution images of graphite in solution conditions while sweeping the field from 0 to 10 T, illustrating the new STM's immunity to magnetic fields. The sub-molecular images of active antibodies and plasmid DNA in solution conditions show the device's capability of imaging biomolecules. Our STM is suitable for studying chemical molecules and active biomolecules in high magnetic fields.

A Cryostat Applicable to Long-Wavelength Light-Driven Scanning Probe Microscopy.

Recently, there has been growing interest in using lightwave-driven scanning probe microscopy (LD-SPM) to break through the Abbe diffraction limit of focusing, yielding insight into various energy couplings and conversion processes and revealing the internal information of matter. We describe a compact and efficient optical cryostat designed for LD-SPM testing under magnetic fields. The exceptional multilayer radiation shielding insert (MRSI) forms an excellent temperature gradient when filled with heat conducting gas, which removes the requirement to install an optical window in the liquid helium cooling shell. This not only critically avoids the vibration and thermal drift caused by solid heat conduction but also minimizes light transmission loss. The application of gate valves and bellows allows a simpler and more effective replacement of the sample and working cell in the test cavity. ANSYS software is used for steady-state thermal analysis of the MRSI to obtain the temperature distribution and heat transfer rate, and the necessity of the flexible copper shielding strips is illustrated by the simulations. The topography and magnetic domain images of 45 nm-thick La0.67Ca0.33MnO3 thin films on NdGaO3(001) substrates under a magnetic field were obtained by a self-made lightwave-driven magnetic force microscope in this cryostat. The resolution and noise spectra during imaging reveal temperature stability and low vibration throughout the cryostat. The experience acquired during the development of this cryostat will help to establish cryostats of similar types for a variety of optic applications requiring the use of cryogenic temperatures.