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1.
Nanomedicine ; 50: 102671, 2023 06.
Artículo en Inglés | MEDLINE | ID: mdl-37054805

RESUMEN

OBJECTIVE: Perineural invasion (PNI) is associated with local recurrence, distant metastasis, and a poor prognosis in pancreatic cancer. However, rare attempt was made to identified the PNI intraoperative. To facilitate precise R0 excision of the tumor, we planned to develop a fluorescent probe for intraoperative imaging of the PNI using GAP-43 as the target and indocyanine green (ICG) as the carrier. METHODS: The probe was created by binding peptide antibody and ICG. Its targeting was tested in vitro and in vivo using a co-culture model of PC12 and tumor cells to create an in vitro neural invasion model and a mouse sciatic nerve invasion model. The small animal imaging system and surgical navigation system confirmed the probe's potential clinical applicability. The sciatic nerve damage model was created to confirm the probe's targeting. RESULTS: We used the pancreatic cancer samples and the public database to confirm that GAP-43 was preferentially overexpressed in pancreatic cancer, particularly in PNI. PC12 cells showed high GAP-43RA-PEG-ICG probe-specific absorption after being co-cultured with tumor cells in vitro. In the sciatic nerve invasion experiment, animals in probe group displayed a significantly stronger fluorescence signal at the PNI compared to ICG-NP and the contralateral normal nerves groups. Although only 60 % of mice appeared to have R0 resections by the naked eye, small animal imaging systems and surgical fluorescence navigation systems could remove the tumor with R0 precision. The injury model used in the probe imaging experimental trials demonstrated that the probe was specifically targeted to the injured nerve, regardless of whether the injury was infiltrated by a tumor or physical. CONCLUSION: We developed the GAP-43Ra-ICG-PEG, an active-targeting near-infrared fluorescent (NIRF) probe, that specifically binds to GAP-43-positive neural cells in an in vitro model of PNI. The probe efficiently visualized PNI lesions in pancreatic cancer in preclinical models, opening up new possibilities for NIRF-guided pancreatic surgery, particularly for PNI patients.


Asunto(s)
Verde de Indocianina , Neoplasias Pancreáticas , Ratas , Ratones , Animales , Colorantes Fluorescentes , Proteína GAP-43 , Neoplasias Pancreáticas/patología , Modelos Animales de Enfermedad , Neoplasias Pancreáticas
2.
Zhongguo Zhong Yao Za Zhi ; 39(23): 4590-5, 2014 Dec.
Artículo en Zh | MEDLINE | ID: mdl-25911807

RESUMEN

In this study, modification technology by surface coating was used to improve the flowability of powder of Chinese herbs extracts. Seven kinds of powder of Chinese herbs extracts were coated with 1% silica nanoparticles using an under-driven Comil. The powder characteristics tester was used to evaluate the flowability of uncoated and coated powders. Surface morphology and particle size distribution of powders were compared by scanning electron microscope (SEM) and optical microscope. The powder hygroscopicity was also investigated. The results showed that, after modification, angle of repose, angle of spatula, compressibility and adhesiveness extremely decreased, and flowability index substantially increased, the powder flowability was significantly improved, especially Gegen and Dahuang extracts powders. Scanning electron microscopy images showed the distribution of silica nanoparticles on the host drug particle surfaces. There were no remarkable changes in the particle size distribution and hygroscopicity of all powders after coating. Therefore, Comilling for surface coating modification technology was an effective method to improve the flowability of Chinese herbs extracts and suggested a possible way forward to enhance the quality of Chinese drugs pharmaceutics in their study and manufacture.


Asunto(s)
Química Farmacéutica/métodos , Medicamentos Herbarios Chinos/química , Nanopartículas/química , Tamaño de la Partícula , Polvos/química , Propiedades de Superficie
3.
J Pediatr Endocrinol Metab ; 37(3): 250-259, 2024 Mar 25.
Artículo en Inglés | MEDLINE | ID: mdl-38332686

RESUMEN

OBJECTIVES: The objective of this study was to develop and evaluate the efficacy of a nomogram for predicting lung metastasis in pediatric differentiated thyroid cancer. METHODS: The SEER database was utilized to collect a dataset consisting of 1,590 patients who were diagnosed between January 2000 and December 2019. This dataset was subsequently utilized for the purpose of constructing a predictive model. The model was constructed utilizing a multivariate logistic regression analysis, incorporating a combination of least absolute shrinkage feature selection and selection operator regression models. The differentiation and calibration of the model were assessed using the C-index, calibration plot, and ROC curve analysis, respectively. Internal validation was performed using a bootstrap validation technique. RESULTS: The results of the study revealed that the nomogram incorporated several predictive variables, namely age, T staging, and positive nodes. The C-index had an excellent calibration value of 0.911 (95 % confidence interval: 0.876-0.946), and a notable C-index value of 0.884 was achieved during interval validation. The area under the ROC curve was determined to be 0.890, indicating its practicality and usefulness in this context. CONCLUSIONS: This study has successfully developed a novel nomogram for predicting lung metastasis in children and adolescent patients diagnosed with thyroid cancer. Clinical decision-making can be enhanced by assessing clinicopathological variables that have a significant predictive value for the probability of lung metastasis in this particular population.


Asunto(s)
Neoplasias Pulmonares , Neoplasias de la Tiroides , Adolescente , Humanos , Niño , Calibración , Toma de Decisiones Clínicas , Bases de Datos Factuales , Estudios Retrospectivos
5.
Chin J Integr Med ; 24(6): 455-459, 2018 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28741063

RESUMEN

OBJECTIVE: To study the effects of Astragalus polysaccharide (APS), the primary effective component of the Chinese herb medicine Astragalus membranaceus (frequently used for its anti-hepatic fibrosis effects), on nanoscale mechanical properties of liver sinusoidal endothelial cells (SECs). METHODS: Using endothelial cell medium as the control, 5 experimental groups were established utilizing different concentrations of APS, i.e. 12.5, 25, 50, 100, and 200 µg/mL. By using atomic force microscopy along with a microcantilever modified with a silicon dioxide microsphere as powerful tools, the value of Young's modulus in each group was calculated. SAS 9.1 software was applied to analyze the values of Young's modulus at the pressed depth of 300 nm. Environmental scanning electron microscopy was performed to observe the surface microtopography of the SECs. RESULTS: The value of Young's modulus in each APS experimental group was significantly greater than that of the control group: as APS concentration increased, the value of Young's modulus presented as an increasing trend. The difference between the low-concentration (12.5 and 25 µg/mL) and high-concentration (200 µg/mL) groups was statistically significant (P<0.05), but no significant differences were observed between moderateconcentration (50 and 100 µg/mL) groups versus low- or high-concentration groups (P>0.05). Surface topography demonstrated that APS was capable of increasing the total area of fenestrae. CONCLUSIONS: The values of Young's modulus increased along with increasing concentrations of APS, suggesting that the stiffness of SECs increases gradually as a function of APS concentration. The observed changes in SEC mechanical properties may provide a new avenue for mechanistic research of anti-hepatic fibrosis treatments in Chinese medicine.


Asunto(s)
Planta del Astrágalo/química , Células Endoteliales/citología , Células Endoteliales/ultraestructura , Hígado/citología , Microscopía de Fuerza Atómica , Nanotecnología , Polisacáridos/farmacología , Animales , Fenómenos Biomecánicos/efectos de los fármacos , Módulo de Elasticidad , Microesferas , Ratas , Dióxido de Silicio/química , Propiedades de Superficie
6.
J Plast Reconstr Aesthet Surg ; 67(11): 1508-14, 2014 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-25087769

RESUMEN

BACKGROUND: The aim of this study was to create a new flap based method for zygomatic cheek defect reconstruction using the pretragal perforator from the superficial temporal artery. METHODS: Ten fresh cadavers were dissected after vascular injection to study pretragal perforator distribution, length, and diameter. Twelve clinical cases of zygomatic cheek defects were reconstructed using pretragal perforator flaps. According to the location of the perforator that was preoperatively probed using an ultrasound Doppler blood flow detector and the zygomatic cheek defect condition, size, and distance from the distal border to the tragus and the flabby surrounding skin, we designed a spindle-shaped longitudinal flap in the preauricular region based on the pretragus. The flap was raised superficially to the superficial musculoaponeurotic system from the caudal border incised primarily to the cephalic border to create the perforator flap. The flap was sutured to the defect and the donor site was closed. RESULTS: Cadaver dissection showed a quite constant perforator given off by the superficial temporal artery or its auricular branches with an appearance rate of 85% anterior to the tragus. The average perforator length was 18.3 mm (range, 11.2-24.2 mm). The average perforator diameter was 0.65 mm (range, 0.4-1.15 mm). A total of 12 clinical cases were available for 6-12 month postoperative follow-up. Favorable survival, primary closure, and esthetic results were achieved without any complications. CONCLUSIONS: Zygomatic cheek defect reconstruction using the above-mentioned flap can achieve satisfactory esthetic and functional results and boasts a simple design, convenient procedure, reliable blood supply, and concealed donor site incision.


Asunto(s)
Mejilla , Colgajo Perforante/irrigación sanguínea , Procedimientos de Cirugía Plástica/métodos , Arterias Temporales/trasplante , Adulto , Anciano , Cadáver , Femenino , Humanos , Masculino , Microcirugia , Persona de Mediana Edad , Ultrasonografía Doppler
7.
Planta ; 215(4): 533-40, 2002 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-12172834

RESUMEN

Floral organs have been successfully induced from the regenerated floral buds of Hyacinthus orientalis L. by precisely controlling exogenous hormones in the medium. Under high concentrations of cytokinin and auxin, the regenerated floral bud produces only tepals. However, at reduced levels of the hormones, the regenerated floral bud can produce stamens and/or carpels with ovules. To understand the molecular mechanism of hormone-regulated flower development, a MADS-box gene, HAG1, which is homologous to AGAMOUS (AG) in Arabidopsis, was isolated from the floral tissues of Hyacinthus. Overexpression of HAG1 in Arabidopsis created flower phenotypes resembling those of the apetala2 mutant and AG transgenic Arabidopsis plants. Furthermore, the HAG1 expression pattern was similar to that of AG, confirming that HAG1 is the ortholog of AG in Hyacinthus. HAG1 mRNA was first detected in cultured explants at day 5 in the medium containing high levels of cytokinin and auxin, which could induce floral regeneration in vitro. However, no HAG1 mRNA was detected in the cultured explants until day 10 in media with low or no hormones. Further, HAG1 mRNA was detected in the stamens and carpels of regenerated floral buds, but not in the tepals. Our data support the hypothesis that hormone-regulated HAG1 activity is required for the induction of floral buds and the determination of floral organ types during the regeneration of floral buds.


Asunto(s)
Adenina/análogos & derivados , Liliaceae/genética , Proteínas de Dominio MADS/genética , Reguladores del Crecimiento de las Plantas/farmacología , Tallos de la Planta/genética , Ácido 2,4-Diclorofenoxiacético/farmacología , Proteína AGAMOUS de Arabidopsis/genética , Adenina/farmacología , Secuencia de Aminoácidos , Arabidopsis/genética , Arabidopsis/crecimiento & desarrollo , Proteínas de Arabidopsis , Compuestos de Bencilo , Diferenciación Celular/efectos de los fármacos , Citocininas/farmacología , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Proteínas de Homeodominio/genética , Ácidos Indolacéticos/farmacología , Cinetina , Liliaceae/efectos de los fármacos , Liliaceae/crecimiento & desarrollo , Proteínas de Dominio MADS/metabolismo , Microscopía Electrónica de Rastreo , Datos de Secuencia Molecular , Proteínas Nucleares/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Tallos de la Planta/crecimiento & desarrollo , Tallos de la Planta/ultraestructura , Plantas Modificadas Genéticamente , Purinas , Homología de Secuencia de Aminoácido
8.
Plant Mol Biol ; 55(2): 209-20, 2004 May.
Artículo en Inglés | MEDLINE | ID: mdl-15604676

RESUMEN

To understand the molecular mechanism of ovule development, a MADS box gene, HoMADS 1 , has been isolated from the ovule tissues of Hyacinthus . Sequence comparison showed that HoMADS 1 is highly homologous to both class C and D genes. Furthermore, phylogenetic analysis suggests that HoMADS 1 is most likely a class D MADS box gene. RNA hybridization revealed that HoMADS 1 was exclusively expressed in the ovules. Over-expressing HoMADS 1 in transgenic Arabidopsis plants produced ectopic carpelloid structures, including ovules, indicating that HoMADS 1 is involved in the determination of carpel and ovule identities. Interestingly, during in vitro flowering, no HoMADS 1 mRNA was detected in the floral tissues at high level hormones in the media. However, HoMADS 1 mRNA accumulated in the floral tissues when the regenerated flowers were transferred to the media containing low level hormones which could induce in vitro ovule formation. Our data suggest that the induction of HoMADS 1 by plant hormones may play important roles during ovule initiation and development in the regenerated flower. Whether HoMADS 1 expression is also regulated by cytokinin and auxin during ovule development in planta remains to be investigated.


Asunto(s)
Flores/genética , Hyacinthus/genética , Proteínas de Dominio MADS/genética , Reguladores del Crecimiento de las Plantas/farmacología , Proteínas de Plantas/genética , Secuencia de Aminoácidos , Arabidopsis/genética , Citocininas/farmacología , ADN Complementario/química , ADN Complementario/genética , ADN Complementario/aislamiento & purificación , Flores/crecimiento & desarrollo , Flores/ultraestructura , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Hyacinthus/efectos de los fármacos , Hyacinthus/crecimiento & desarrollo , Ácidos Indolacéticos/farmacología , Microscopía Electrónica de Rastreo , Datos de Secuencia Molecular , Filogenia , Plantas Modificadas Genéticamente , ARN Mensajero/genética , ARN Mensajero/metabolismo , Alineación de Secuencia , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Técnicas de Cultivo de Tejidos
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