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1.
Fungal Genet Biol ; 100: 13-21, 2017 03.
Artículo en Inglés | MEDLINE | ID: mdl-28089630

RESUMEN

P bodies and stress granules are RNA-containing structures governing mRNA degradation and translational arrest, respectively. Saccharomyces cerevisiae Pbp1 protein localizes to stress granules and promotes their formation and is involved in proper polyadenylation, suppression of RNA-DNA hybrids, and preventing aberrant rDNA recombination. A genetic screen for Aspergillus nidulans mutants aberrant in secondary metabolism identified the Pbp1 homolog, PbpA. Using Dcp1 (mRNA decapping) as a marker for P-body formation and FabM (Pab1, poly-A binding protein) to track stress granule accumulation, we examine the dynamics of RNA granule formation in A. nidulans cells lacking pub1, edc3, and pbpA. Although PbpA acts as a functional homolog of yeast PBP1, PbpA had little impact on either P-body or stress granule formation in A. nidulans in contrast to Pub1 and Edc3. However, we find that PbpA is critical for sexual development and its loss increases the production of some secondary metabolites including the carcinogen sterigmatocystin.


Asunto(s)
Aspergillus nidulans/genética , Proteínas Portadoras/genética , Proteínas de Saccharomyces cerevisiae/genética , Metabolismo Secundario/genética , Desarrollo Sexual/genética , Gránulos Citoplasmáticos/metabolismo , Unión Proteica , Biosíntesis de Proteínas , Estabilidad del ARN/genética , Saccharomyces cerevisiae/genética , Esterigmatocistina/biosíntesis
2.
Phys Med Rehabil Clin N Am ; 32(2): 355-372, 2021 05.
Artículo en Inglés | MEDLINE | ID: mdl-33814062

RESUMEN

Telerehabilitation for pain management uses communication technology to minimize geographic barriers. Access to such technology has proven critically important during the coronavirus disease-2019 pandemic and has been useful for patients with chronic pain disorders unable to travel. The evaluation and treatment of such disorders requires a whole health approach that individualizes treatment options and delivers care through a biopsychosocial approach. The goals of care are unchanged from an in-person patient-provider experience. Telerehabilitation can be successfully implemented in pain management with appropriate consideration for staging an evaluation, a structured approach to the visit, and application of standard clinical metrics.


Asunto(s)
Dolor Crónico/tratamiento farmacológico , Dolor Crónico/rehabilitación , Manejo del Dolor/métodos , Telemedicina/métodos , COVID-19/epidemiología , Terapia Cognitivo-Conductual , Accesibilidad a los Servicios de Salud , Humanos , Dimensión del Dolor , Pandemias , Examen Físico , Modalidades de Fisioterapia , SARS-CoV-2
3.
Phys Med Rehabil Clin N Am ; 32(2): 263-276, 2021 05.
Artículo en Inglés | MEDLINE | ID: mdl-33814057

RESUMEN

Cardiopulmonary telerehabilitation is a safe and effective alternative to traditional center-based rehabilitation. It offers a sustainable solution to more conveniently meet the needs of patients with acute or chronic, preexisting or newly acquired, cardiopulmonary diseases. To maximize success, programs should prioritize basic, safe, and timely care options over comprehensive or complex approaches. The future should incorporate new strategies learned during a global pandemic and harness the power of information and communication technology to provide evidence-based patient-centered care. This review highlights clinical considerations, current evidence, recommendations, and future directions of cardiopulmonary telerehabilitation.


Asunto(s)
Rehabilitación Cardiaca/métodos , Accesibilidad a los Servicios de Salud , Terapia Respiratoria/métodos , Telerrehabilitación/métodos , COVID-19/epidemiología , Rehabilitación Cardiaca/economía , Humanos , Pandemias , Terapia Respiratoria/economía , SARS-CoV-2 , Telerrehabilitación/economía , Estados Unidos/epidemiología
4.
Rev Recent Clin Trials ; 16(3): 288-293, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33267764

RESUMEN

BACKGROUND: Severe postoperative pain is principally managed by opioids. While effective, opioids do not provide adequate relief in many patients and cause many side effects, including antinociceptive tolerance and opioid-induced hyperalgesia. To evaluate if a combination of intravenous Magnesium, Lidocaine, Ketorolac (MLK cocktail) is a useful rescue therapy through synergistic pharmacological mechanisms for acute pain relief. We present the intravenous combination of magnesium, lidocaine, and ketorolac (MLK cocktail) as a possible rescue for opioid insensitive severe post-operative pain. MATERIALS AND METHODS: The principal settings were the post-operative care unit (PACU) and the surgical ward. We retrospectively analyzed the electronic medical record and anesthesia documents of 14 patients experiencing severe postoperative pain, >7/10 visual-analogue pain score (VAS), despite receiving at least 8 mg of intravenous morphine milligram equivalents (MME) after arrival in the LAC+USC Medical Center PACU between September 2012 and January 2013. The data reviewed included patients' demographics, disease etiology, surgical procedure, opioids received perioperatively, and visual-analogue pain scores before and after each analgesic received, and after the MLK cocktail. The a priori primary outcome and a posteriori secondary outcome of this study are mean visual-analogue pain score and morphine milligram equivalent dose administered per hour, respectively. The main tool evaluated has been VAS score. RESULTS: In patients who failed to respond to opioid analgesics, administration of the MLK cocktail improved the VAS pain scores immediately from 9.4 ± 1.0 to 3.6 ± 3.5. The MLK cocktail also decreased the MME doses/hour in the immediate 12 hours postoperative period from 12.4 ± 5.6 to 1.1 ± 0.9. CONCLUSION: In patients experiencing opioid-resistant severe postoperative pain, the magnesium, lidocaine, and ketorolac combination may be an effective nonopioid rescue therapy. Additionally, magnesium, lidocaine, and ketorolac may be utilized in cases complicated by either antinociceptive tolerance or opioid-induced hyperalgesia and can restore opioid responsiveness.


Asunto(s)
Analgésicos Opioides , Ketorolaco , Antiinflamatorios no Esteroideos/uso terapéutico , Método Doble Ciego , Humanos , Ketorolaco/uso terapéutico , Lidocaína/uso terapéutico , Magnesio/uso terapéutico , Dolor Postoperatorio/tratamiento farmacológico , Estudios Retrospectivos
5.
PLoS One ; 8(12): e82184, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24358152

RESUMEN

Rad54 is a dsDNA-dependent ATPase that translocates on duplex DNA. Its ATPase function is essential for homologous recombination, a pathway critical for meiotic chromosome segregation, repair of complex DNA damage, and recovery of stalled or broken replication forks. In recombination, Rad54 cooperates with Rad51 protein and is required to dissociate Rad51 from heteroduplex DNA to allow access by DNA polymerases for recombination-associated DNA synthesis. Sequence analysis revealed that Rad54 contains a perfect match to the consensus PIP box sequence, a widely spread PCNA interaction motif. Indeed, Rad54 interacts directly with PCNA, but this interaction is not mediated by the Rad54 PIP box-like sequence. This sequence is located as an extension of motif III of the Rad54 motor domain and is essential for full Rad54 ATPase activity. Mutations in this motif render Rad54 non-functional in vivo and severely compromise its activities in vitro. Further analysis demonstrated that such mutations affect dsDNA binding, consistent with the location of this sequence motif on the surface of the cleft formed by two RecA-like domains, which likely forms the dsDNA binding site of Rad54. Our study identified a novel sequence motif critical for Rad54 function and showed that even perfect matches to the PIP box consensus may not necessarily identify PCNA interaction sites.


Asunto(s)
Adenosina Trifosfatasas/metabolismo , ADN Helicasas/metabolismo , Enzimas Reparadoras del ADN/metabolismo , Reparación del ADN , Proteínas de Saccharomyces cerevisiae/metabolismo , Factores de Transcripción/metabolismo , Adenosina Trifosfatasas/genética , Secuencia Conservada , Daño del ADN , ADN Helicasas/genética , Enzimas Reparadoras del ADN/genética , Recombinasa Rad51/genética , Recombinasa Rad51/metabolismo , Recombinación Genética , Saccharomyces cerevisiae , Proteínas de Saccharomyces cerevisiae/genética , Factores de Transcripción/genética
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