RESUMEN
Drought resistance in plants is influenced by multiple signaling pathways that involve various transcription factors, many target genes, and multiple types of epigenetic modifications. Studies on epigenetic modifications of drought focus on DNA methylation and histone modifications, with fewer on chromatin remodeling. Changes in chromatin accessibility can play an important role in abiotic stress in plants by affecting RNA polymerase binding and various regulatory factors. However, the changes in chromatin accessibility during drought in apples are not well understood. In this study, the landscape of chromatin accessibility associated with the gene expression of apple (GL3) under drought conditions was analyzed by Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq) and RNA-seq. Differential analysis between drought treatment and control identified 23,466 peaks of upregulated chromatin accessibility and 2447 peaks of downregulated accessibility. The drought-induced chromatin accessibility changed genes were mainly enriched in metabolism, stimulus, and binding pathways. By combining results from differential analysis of RNA-seq and ATAC-seq, we identified 240 genes with higher chromatin accessibility and increased gene expression under drought conditions that may play important functions in the drought response process. Among them, a total of nine transcription factor genes were identified, including ATHB7, HAT5, and WRKY26. These transcription factor genes are differentially expressed with different chromatin accessibility motif binding loci that may participate in apple response to drought by regulating downstream genes. Our study provides a reference for chromatin accessibility under drought stress in apples and the results will facilitate subsequent studies on chromatin remodelers and transcription factors.
Asunto(s)
Secuenciación de Inmunoprecipitación de Cromatina , Malus , Cromatina/genética , ARN Polimerasas Dirigidas por ADN/genética , Sequías , Expresión Génica , Secuenciación de Nucleótidos de Alto Rendimiento , Malus/genética , Malus/metabolismo , RNA-Seq , Factores de Transcripción/genética , Transposasas/genéticaRESUMEN
Prunus davidiana (Rosaceae) is of great importance horticulturally as the rootstock of some fruit trees. Here, the complete chloroplast genome of P. davidiana was assembled based on the Illumina reads. The complete cp genome of P. davidiana was 157,660 bp in length and contained a pair of inverted repeat (IR, 26,387 bp) regions, which were separated by the small single-copy (SSC, 19,122 bp) and the large single-copy (LSC, 85,764 bp) regions. It encoded 135 genes. The phylogenetic analysis revealed that P. davidiana is sister to other peaches. This result will be useful for future studies of the lineage.