RESUMEN
Influenza A virus (IAV) is an important pathogen that poses a severe threat to the health of humans. Nucleoprotein (NP) of IAV plays crucial roles in the viral life cycle by interacting with various cellular factors. Histone Acetyl Transferase TIP60 is a key target of several viral proteins during infection, including HIV-1 Tat, HPV E6, HTLV-1 p30II and HCMV UL27 proteins. However, Whether the interaction between the IAV NP and TIP60, and the role of TIP60 in IAV life cycle are largely unknown. Here, we showed that IAV infection up-regulated TIP60 protein and RNA expression. Overexpression of TIP60 inhibited viral protein and RNA expression and reduced the progeny viral titer. Further study revealed that TIP60 inhibited viral replication through activation of TBK1-IRF3 signaling pathway. Furthermore, we demonstrated that the NP protein of IAV interacted with TIP60. Together, these results indicate that TIP60 play a repressor in IAV infection, and it may be a possible target for antiviral drugs.
Asunto(s)
Interacciones Huésped-Patógeno , Virus de la Influenza A/fisiología , Lisina Acetiltransferasa 5/metabolismo , Proteínas de Unión al ARN/metabolismo , Transducción de Señal , Proteínas del Núcleo Viral/metabolismo , Replicación Viral , Células A549 , Replicación del ADN , Células HEK293 , Humanos , Virus de la Influenza A/genética , Gripe Humana , Factor 3 Regulador del Interferón/genética , Factor 3 Regulador del Interferón/metabolismo , Lisina Acetiltransferasa 5/genética , Proteínas de la Nucleocápside , Proteínas Serina-Treonina Quinasas/genética , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas de Unión al ARN/genética , Regulación hacia Arriba , Proteínas del Núcleo Viral/genéticaRESUMEN
The highly pathogenic avian influenza (HPAI) A/H5N1 virus hijacks host cellular machinery to complete its life cycle; identification of the host factors involved in viral replication may facilitate antiviral drug development. Here, we first characterize a metastasis suppressor, N-myc downstream-regulated gene 1 (NDRG1), and showed that it plays a crucial role in H5N1 viral replication. We found that H5N1 infection upregulated NDRG1 mRNA and protein expression. Overexpression of NDRG1 released approximately 4-fold more virions compared to the control group, whereas knockdown of NDRG1 resulted in a drop in viral RNA and protein production. Further investigation revealed that NDRG1 facilitated HPAI A/H5N1 viral replication by suppressing the canonical NF-κB signaling pathway. Furthermore, our results also showed that the NDRG1 mRNA level was mainly stimulated by M1 and PB1 viral proteins. Overall, our results suggest that NDRG1 plays a positive role in HPAI replication by suppressing the canonical NF-κB signaling pathway.
Asunto(s)
Proteínas de Ciclo Celular/metabolismo , Subtipo H5N1 del Virus de la Influenza A/fisiología , Péptidos y Proteínas de Señalización Intracelular/metabolismo , FN-kappa B/metabolismo , Replicación Viral , Células A549 , Animales , Proteínas de Ciclo Celular/genética , Replicación del ADN , Técnicas de Silenciamiento del Gen , Interacciones Huésped-Patógeno , Humanos , Quinasa I-kappa B/metabolismo , Subtipo H5N1 del Virus de la Influenza A/patogenicidad , Péptidos y Proteínas de Señalización Intracelular/genética , ARN Viral/genética , Transducción de Señal , Regulación hacia ArribaRESUMEN
Vibrio vulnificus is a Gram-negative, curved, obligate halophilic marine bacterium that exclusively exists in coastal seawaters. Previous studies revealed that V. vulnificus is one of the most dangerous foodborne zoonotic pathogens for human beings. However, it remains unknown whether marine mammals can be infected by V. vulnificus. In May 2016, a captive spotted seal (Phoca largha) died due to septicemia induced by V. vulnificus. Upon post-mortem examination, V. vulnificus was isolated, identified, and named as BJ-PH01. Further analysis showed that BJ-PH01 belongs to biotype 1 and the Clinical genotype. Furthermore, we performed an epidemiological investigation of V. vulnificus in six aquariums in northern China. As a result, V. vulnificus was successfully isolated from all investigated aquariums. The positive rates ranged from 20% to 100% in each investigated aquarium. During the investigation, 12 strains of V. vulnificus were isolated, and all 12 isolates were classified into biotype 1. Eleven of the 12 isolates belonged to the Clinical genotype, and one isolate belonged to the Environmental genotype. All 12 isolated V. vulnificus strains showed limited antibiotic resistance. Overall, our work demonstrated that V. vulnificus is frequently distributed in aquariums, thus constituting a threat to captive marine mammals and to public health.
Asunto(s)
Enfermedades de los Animales/microbiología , Animales de Zoológico/microbiología , Phocidae/microbiología , Vibriosis/veterinaria , Vibrio vulnificus/aislamiento & purificación , Enfermedades Transmitidas por el Agua/veterinaria , Animales , China , Peces/microbiología , Genotipo , Mamíferos , Salud Pública , Vibriosis/microbiología , Vibrio vulnificus/patogenicidad , Microbiología del Agua , Enfermedades Transmitidas por el Agua/microbiologíaRESUMEN
H5N1 is a highly pathogenic influenza A virus (IAV) and poses a major threat to the public health. The nucleoprotein (NP) has a multiple functions during the viral life cycle, however, the precise role of NP mutants in viral replication and pathogenicity is not completely understood. Here, we attempted to identify five residues in NP that may contribute to viral replication or pathogenicity. Of these, K227R, K229R, and K470R viruses were successfully rescued by reverse genetic, but the K91R and K198R viruses were not viable. A mini-genome assay demonstrated that the NP mutations K91R and K198R significantly decreased the polymerase activity. Moreover, these two mutations resulted in disrupted cellular localization in mammalian cells. Importantly, mutation at position 470 of NP significantly increased its virulence in vitro and in vivo. These findings demonstrated that the NP protein plays a major role in influenza virulence and pathogenicity, which adds to the knowledge of IAV virulence determinants and may benefit IAV surveillance.