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1.
BMC Endocr Disord ; 24(1): 146, 2024 Aug 09.
Artículo en Inglés | MEDLINE | ID: mdl-39123156

RESUMEN

BACKGROUND: In recent years, the incidence of thyroid nodules has increased significantly. There are various ways to treat thyroid nodules, and ablation therapy is one of the important ways to treat thyroid nodules. However, there are many complications and deficiencies in the current ablation treatment of thyroid nodules, especially the incomplete ablation of thyroid cancer nodules, which limits the further application of ablation technology. In this paper, we report two cases of incomplete ablation of thyroid nodules, one of which underwent surgical treatment due to anxiety after ablation, and the postoperative pathology confirmed that there was still residual papillary thyroid carcinoma, and the other patient underwent an operation after ablation, but visited our medical institution again due to cervical lymph node metastasis in a short period of time, and after radical cervical lymph node dissection, pathology confirmed multiple cervical lymph node metastasis. Radionuclide therapy was performed after surgery, and two patients are currently receiving endocrine suppression therapy, and their condition is stable with no signs of recurrence. CONCLUSION: The incomplete ablation of thyroid cancer nodules limits the development of ablation therapy, making ablation treatment a double-edged sword. Guidelines and expert consensus can guide their development, but they need to evolve with the times, and a multidisciplinary diagnostic team can help screen the most suitable patients. Only by using this technology more standardly, using the most appropriate technology, and treating the most suitable patients, can benefit more and more patients.


Asunto(s)
Neoplasias de la Tiroides , Humanos , Neoplasias de la Tiroides/cirugía , Neoplasias de la Tiroides/patología , Femenino , Persona de Mediana Edad , Masculino , Adulto , Radioisótopos de Yodo/uso terapéutico , Cáncer Papilar Tiroideo/cirugía , Cáncer Papilar Tiroideo/patología , Nódulo Tiroideo/cirugía , Nódulo Tiroideo/patología , Técnicas de Ablación/métodos , Metástasis Linfática
2.
Biochem Genet ; 62(2): 718-740, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-37410307

RESUMEN

To investigate the function of ten-eleven translocation 1 (TET1) and its underlying mechanism in papillary thyroid cancer (PTC). Using the RNA-Seq data based on GDC TCGA, we analyzed the gene expression pattern of TET1 in PTC. Immunohistochemistry was carried out to assess the TET1 protein level. Then, its diagnostic and prognostic functions were determined by various bioinformatics approaches. Enrichment analysis was performed to explore the potential pathways in which TET1 is mainly involved. Finally, the immune cell infiltration analysis was conducted and the association of TET1 mRNA expression with the expression levels of immune checkpoints, tumor mutation burden (TMB) score, microsatellite instability (MSI) score, and cancer stem cells (CSC) score was examined. TET1 expression was lower in PTC tissues compared with that in normal tissues (P < 0.01). Besides, TET1 had a certain value in diagnosing PTC, and low-TET1 mRNA expression led to favorable disease-specific survival (DSS) (P < 0.01). The enrichment analysis revealed autoimmune thyroid disease and cytokine-cytokine receptor interaction were the consistent pathways in which TET1 participated. TET1 was negatively correlated with the Stromal score and Immune score. The different proportions of immune cell subtypes were observed between high- and low-TET1 expression groups. Interestingly, TET1 mRNA expression was inversely related to the expression levels of immune checkpoints, and TMB, MSI, and CSC scores. TET1 might be a robust diagnostic and prognostic biomarker for PTC. TET1 affected the DSS of PTC patients possibly through the regulation of immune-related pathways and tumor immunity.

3.
Eur J Anaesthesiol ; 41(3): 226-233, 2024 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-38230449

RESUMEN

BACKGROUND: Sleep disturbances in the peri-operative period have been associated with adverse outcomes, including postoperative delirium (POD). However, research on sleep quality during the immediate postoperative period is limited. OBJECTIVES: This study aimed to investigate the association between sleep quality on the night of the operative day assessed using the Sleep Quality Numeric Rating Scale (SQ-NRS), and the incidence of POD in a large cohort of surgical patients. DESIGN: A prospective cohort study. SETTING: A tertiary hospital in China. PATIENTS: This study enrolled patients aged 65 years or older undergoing elective surgery under general anaesthesia. The participants were categorised into the sleep disturbance and no sleep disturbance groups according to their operative night SQ-NRS. MAIN OUTCOME MEASURES: The primary outcome was delirium incidence, whereas the secondary outcomes included acute kidney injury, stroke, pulmonary infection, cardiovascular complications and all-cause mortality within 1 year postoperatively. RESULTS: In total, 3072 patients were included in the analysis of this study. Among them, 791 (25.72%) experienced sleep disturbances on the night of operative day. Patients in the sleep disturbance group had a significantly higher risk of developing POD (adjusted OR 1.43, 95% CI 1.11 to 1.82, P  = 0.005). Subgroup analysis revealed that age 65-75 years; male sex; ASA III and IV; haemoglobin more than 12 g l -1 ; intra-operative hypotension; surgical duration more than 120 min; and education 9 years or less were significantly associated with POD. No interaction was observed between the subgroups. No significant differences were observed in the secondary outcomes, such as acute kidney injury, stroke, pulmonary infection, cardiovascular complications and all-cause mortality within 1 year postoperatively. CONCLUSIONS: The poor subjective sleep quality on the night of operative day was independently associated with increased POD risk, especially in certain subpopulations. Optimising peri-operative sleep may reduce POD. Further research should investigate potential mechanisms and causal relationships. TRIAL REGISTRY: chictr.org.cn: ChiCTR1900028545.


Asunto(s)
Lesión Renal Aguda , Infecciones Cardiovasculares , Delirio , Delirio del Despertar , Accidente Cerebrovascular , Anciano , Humanos , Masculino , Infecciones Cardiovasculares/complicaciones , Delirio/diagnóstico , Delirio/epidemiología , Delirio/etiología , Delirio del Despertar/diagnóstico , Delirio del Despertar/epidemiología , Delirio del Despertar/etiología , Complicaciones Posoperatorias/diagnóstico , Complicaciones Posoperatorias/epidemiología , Complicaciones Posoperatorias/etiología , Estudios Prospectivos , Factores de Riesgo , Calidad del Sueño , Femenino
4.
J Cell Biochem ; 120(6): 9850-9858, 2019 06.
Artículo en Inglés | MEDLINE | ID: mdl-30681200

RESUMEN

Whitmania pigra is a unique, fluid-sucking ectoparasite and an anticoagulant medical leech. The codon usage bias (CUB) is the nonuniform usage of synonymous codons in which some codons are more preferred than others. Here, we performed a comprehensive analysis of CUB of genes in W. pigra, analyzing 140 780 transcripts, 59 553 unigenes, and 20 304 qualified coding sequences (CDSs) from the transcriptomic data of W. pigra. The effective number of codons values suggested that the CUB was low in these genes. We recognized profoundly favored codons in W. pigra that have a G/C-ending. Parity rule two-bias plots suggested that both mutation pressure and natural selection might have influenced the CUB. However, neutrality plots revealed that natural selection might have played a major role while mutation pressure might have played a minor role in shaping the CUB. We applied principal component analysis to relative synonymous codon usage values for divided CDSs based on GC content and codon-ending bases. Codon usage in W. pigra had a general inclination toward C-ending codons and natural selection rather than mutation pressure is the dominant force in the genetic evolution of W. pigra. To our knowledge, this is the first study to describe a complete codon usage analysis of W. pigra; this will increase the understanding of CUB and evolution in W. pigra. The analysis of codon usage patterns in W. pigra aids in understanding its evolution and genetic architecture.


Asunto(s)
Uso de Codones , Evolución Molecular , Sanguijuelas/genética , Mutación , Selección Genética , Animales
5.
BMC Genomics ; 19(1): 542, 2018 Jul 17.
Artículo en Inglés | MEDLINE | ID: mdl-30016953

RESUMEN

BACKGROUND: Hirudinaria manillensis is an ephemeral, blood-sucking ectoparasite, possessing anticoagulant capacities with potential medical applications. Analysis of codon usage patterns would contribute to our understanding of the evolutionary mechanisms and genetic architecture of H. manillensis, which in turn would provide insight into the characteristics of other leeches. We analysed codon usage and related indices using 18,000 coding sequences (CDSs) retrieved from H. manillensis RNA-Seq data. RESULTS: We identified four highly preferred codons in H. manillensis that have G/C-endings. Points generated in an effective number of codons (ENC) plot distributed below the standard curve and the slope of a neutrality plot was less than 1. Highly expressed CDSs had lower ENC content and higher GC content than weakly expressed CDSs. Principal component analysis conducted on relative synonymous codon usage (RSCU) values divided CDSs according to GC content and divided codons according to ending bases. Moreover, by determining codon usage, we found that the majority of blood-diet related genes have undergone less adaptive evolution in H. manillensis, except for those with homologous sequences in the host species. CONCLUSIONS: Codon usage in H. manillensis had an overall preference toward C-endings and indicated that codon usage patterns are mediated by differential expression, GC content, and biological function. Although mutation pressure effects were also notable, the majority of genetic evolution in H. manillensis was driven by natural selection.


Asunto(s)
Codón , Evolución Molecular , Sanguijuelas/genética , Selección Genética , Animales , Composición de Base , Expresión Génica , Genoma , Sanguijuelas/metabolismo , Nucleótidos/análisis , Análisis de Componente Principal , Proteínas/química , Proteínas/genética
6.
J Anim Ecol ; 83(5): 1149-57, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24666375

RESUMEN

A trophic cascade occurs when predators directly decrease the densities, or change the behaviour, of herbivores and thus indirectly increase plant productivity. The predator-herbivore-plant context is well known, but some predators attack species beneficial to plants (e.g. pollinators) and/or enemies of herbivores (e.g. parasites), and their role in the dynamics of mutualisms remains largely unexplored. We surveyed the predatory ant species and studied predation by the dominant ant species, the weaver ant Oecophylla smaragdina, associated with the fig tree Ficus racemosa in southwest China. We then tested the effects of weaver ants on the oviposition behaviour of pollinating and non-pollinating fig wasps in an ant-exclusion experiment. The effects of weaver ants on fig wasp community structure and fig seed production were then compared between trees with and without O. smaragdina. Oecophylla smaragdina captured more non-pollinating wasps (Platyneura mayri) than pollinators as the insects arrived to lay eggs. When ants were excluded, more non-pollinators laid eggs into figs and fewer pollinators entered figs. Furthermore, trees with O. smaragdina produced more pollinator offspring and fewer non-pollinator offspring, shifting the community structure significantly. In addition, F. racemosa produced significantly more seeds on trees inhabited by weaver ants. Oecophylla smaragdina predation reverses the dominance of the two commonest wasp species at the egg-laying stage and favours the pollinators. This behavioural pattern is mirrored by wasp offspring production, with pollinators' offspring dominating figs produced by trees inhabited by weaver ants, and offspring of the non-pollinator P. mayri most abundant in figs on trees inhabited by other ants. Overall, our results suggest that predation by weaver ants limits the success of the non-pollinating P. mayri and therefore indirectly benefits the mutualism by increasing the reproductive success of both the pollinators and the plant. Predation is thus a key functional factor that can shape the community structure of a pollinator-plant mutualistic system.


Asunto(s)
Hormigas/fisiología , Ficus/parasitología , Cadena Alimentaria , Polinización , Avispas/fisiología , Animales , China , Femenino , Ficus/fisiología , Interacciones Huésped-Parásitos , Oviposición/fisiología , Conducta Predatoria , Semillas/parasitología , Simbiosis
7.
Genome Biol Evol ; 16(7)2024 Jul 03.
Artículo en Inglés | MEDLINE | ID: mdl-38946321

RESUMEN

Oecanthus is a genus of cricket known for its distinctive chirping and distributed across major zoogeographical regions worldwide. This study focuses on Oecanthus rufescens, and conducts a comprehensive examination of its genome through genome sequencing technologies and bioinformatic analysis. A high-quality chromosome-level genome of O. rufescens was successfully obtained, revealing significant features of its genome structure. The genome size is 877.9 Mb, comprising ten pseudo-chromosomes and 70 other sequences, with a GC content of 41.38% and an N50 value of 157,110,771 bp, indicating a high level of continuity. BUSCO assessment results demonstrate that the genome's integrity and quality are high (of which 96.8% are single-copy and 1.6% are duplicated). Comprehensive genome annotation was also performed, identifying approximately 310 Mb of repetitive sequences, accounting for 35.3% of the total genome sequence, and discovering 15,481 tRNA genes, 4,082 rRNA genes, and 1,212 other noncoding genes. Furthermore, 15,031 protein-coding genes were identified, with BUSCO assessment results showing that 98.4% (of which 96.3% are single-copy and 1.6% are duplicated) of the genes were annotated.


Asunto(s)
Genoma de los Insectos , Anotación de Secuencia Molecular , Animales , Cromosomas de Insectos/genética , Gryllidae/genética , Ortópteros/genética , Ortópteros/clasificación
8.
Endokrynol Pol ; 75(3): 262-266, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38887113

RESUMEN

The incidence of thyroid nodules is rising annually. Surgical treatment is effective, but often results in significant trauma, recurrent laryngeal nerve injury, hypoparathyroidism, and other complications. Recent years have seen significant breakthroughs in thyroid nodule ablation for treating thyroid diseases, although its application remains controversial. The objective was to review the development history and current research status of thyroid nodule ablation to provide a reference for future studies. The literature on thyroid nodule ablation was reviewed, analysing its advantages and disadvantages. The therapeutic effect of thyroid nodule ablation in treating benign thyroid lesions is noteworthy, but issues such as lax treatment indications and excessive medical treatment persist. Initial success has been achieved in treating thyroid malignant lesions, particularly papillary thyroid microcarcinoma (PTMC). However, the curative effect requires further follow-up verification.


Asunto(s)
Nódulo Tiroideo , Humanos , Nódulo Tiroideo/cirugía , Nódulo Tiroideo/patología , Neoplasias de la Tiroides/cirugía , Neoplasias de la Tiroides/patología , Femenino , Técnicas de Ablación/métodos , Masculino , Resultado del Tratamiento
9.
Int J Surg ; 110(1): 219-228, 2024 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-37738004

RESUMEN

BACKGROUND: Identifying the risk factors associated with perioperative mortality is crucial, particularly in older patients. Predicting 6-month mortality risk in older patients based on large datasets can assist patients and surgeons in perioperative clinical decision-making. This study aimed to develop a risk prediction model of mortality within 6 months after noncardiac surgery using the clinical data from 11 894 older patients in China. MATERIALS AND METHODS: A multicentre, retrospective cohort study was conducted in 20 tertiary hospitals. The authors retrospectively included 11 894 patients (aged ≥65 years) who underwent noncardiac surgery between April 2020 and April 2022. The least absolute shrinkage and selection operator model based on linear regression was used to analyse and select risk factors, and various machine learning methods were used to build predictive models of 6-month mortality. RESULTS: The authors predicted 12 preoperative risk factors associated with 6-month mortality in older patients after noncardiac surgery. Including laboratory-associated risk factors such as mononuclear cell ratio and total blood cholesterol level, etc. Also including medical history associated risk factors such as stroke, history of chronic diseases, etc. By using a random forest model, the authors constructed a predictive model with a satisfactory accuracy (area under the receiver operating characteristic curve=0.97). CONCLUSION: The authors identified 12 preoperative risk factors associated with 6-month mortality in noncardiac surgery older patients. These preoperative risk factors may provide evidence for a comprehensive preoperative anaesthesia assessment as well as necessary information for clinical decision-making by anaesthesiologists.


Asunto(s)
Accidente Cerebrovascular , Humanos , Anciano , Estudios Retrospectivos , Medición de Riesgo/métodos , Factores de Riesgo , Toma de Decisiones Clínicas
10.
Zootaxa ; 5339(4): 390-396, 2023 Aug 31.
Artículo en Inglés | MEDLINE | ID: mdl-38221045

RESUMEN

A new species, Polionemobius gyirongensis Ma & Wang, sp. nov., is reported from Tibet, China. Its appearance is similar to that of Polionemobius yunnanus Liu & Shi, 2014, with a small-sized body and oval notch on both sides of the pronotum. Description and illustrations for the new species are provided here. At the same time, we compared Polionemobius ebony Wu & Ma, 2022 and Pteronemobius nigriscens (Shiraki, 1911) and determined that they are different species.


Asunto(s)
Gryllidae , Ortópteros , Animales , Tibet , Distribución Animal , China , Tamaño Corporal , Estructuras Animales , Tamaño de los Órganos
11.
Zootaxa ; 5336(1): 135-140, 2023 Aug 21.
Artículo en Inglés | MEDLINE | ID: mdl-38221106

RESUMEN

The species with a strongly pubescent body with the genus name Capillogryllus by Xie, Zheng, and Liang in 2003 are reviewed. Capillogryllus has presently two species, Capillogryllus exilipalpis Xie & Zheng, 2003 (type specimen is a female), and Capillogryllus dolabripalpis Xie & Zheng, 2003 (type species), both of which are only known from China at this time. In Gyirong County, Tibet, we discovered a new species of this genus that is distinct from the two species mentioned above. Here, three species of Capillogryllus are described and illustrated.


Asunto(s)
Gryllidae , Ortópteros , Femenino , Animales , Distribución Animal , Tamaño de los Órganos , Estructuras Animales , Tamaño Corporal , China
12.
Zootaxa ; 5360(2): 269-278, 2023 Oct 26.
Artículo en Inglés | MEDLINE | ID: mdl-38220612

RESUMEN

Dark body color is very common among crickets, and this article discusses four species of black field crickets with distinct distributions. There are three species of the genus Melanogryllus Chopard, 1961 and one species of the genus Velarifictorus Randell, 1964. Of them, Melanogryllus chopardi Bey-Bienko, 1968 was discovered in Southwest Xizang, on the border between China and Nepal; Melanogryllus desertus (Pallas, 1771) was primarily found in arid regions of northwestern China; Melanogryllus bilineatus Yang & Yang, 1994 was often collected from the Southeast China coastal region; and the last species, Velarifictorus yuanilandrevus sp. nov., is a new species that was collected in Yunnan, a province in Southwest China. In addition, the new species could be distinguished from the others by its black body coloration and the absence of yellow stripes on the head. Here, we describe these species in detail, with photographs of the genitalia, and provide a distribution map for them and keys for Chinese Melanogryllus species.


Asunto(s)
Gryllidae , Ortópteros , Animales , China , Distribución Animal , Tamaño Corporal , Tamaño de los Órganos , Estructuras Animales
13.
Zootaxa ; 5380(1): 56-66, 2023 Nov 28.
Artículo en Inglés | MEDLINE | ID: mdl-38220791

RESUMEN

The digestive tract of insects from mouth to anus is the crop, proventriculus, gastric caecum, midgut, ileum, colon and rectum, and the Malpighian tubules attached to the ileum belong to the excretory organs. As the main organs for digesting food and absorbing nutrients, the structure of the insect digestive tract is inevitably adapted to feeding habits. Among the Orthoptera, the digestive tracts of phytophagous locusts and carnivorous katydids have been studied for their adaptations to their food habits, while the adaptations of the digestive tracts of omnivorous insects have been less studied. In order to systematically study the adaptations of the digestive tracts of ground-nesting omnivorous insects to their feeding habits, this study was carried out with Loxoblemmus taicoun Saussure, 1877, Teleogryllus emma (Ohmachi & Matsuura, 1951), and Velarifictorus micado (Saussure, 1877). Through the use of a scanning electron microscope (SEM) and a VHX digital microscope, the digestive tract structures of these three most widespread and common omnivorous crickets in China were examined in order to better understand how omnivorous insects digestive tract structures differ from those of specialists. When we compared the morphological features of these crickets digestive tracts to those of phytophagous and carnivorous insects, we discovered six adaptive traits of omnivorous insects, including: (1) they have a shorter foregut and longer midgut and hindgut; (2) they have a well-developed muscular intestinal wall; (3) the spines in the inner wall of their crop are uniformly arranged and less chitinized; (4) the proventriculus is sclerotized and spherical, and the inner wall is accompanied by ossified large teeth; (5) they have well-developed cilia at the base of the crop and at the base of the ossified denticles; (6) they have only two lobulated gastric caeca. The study summarizes six characteristics of the digestive tract of omnivorous crickets that are compatible with their food habits, providing evidence and clues for further research on the relationship between the digestive tract and food habits in Orthoptera.


Asunto(s)
Tracto Gastrointestinal , Gryllidae , Animales , Tracto Gastrointestinal/anatomía & histología , Insectos , Microscopía , Carnivoría
14.
Zootaxa ; 5330(1): 141-146, 2023 Aug 14.
Artículo en Inglés | MEDLINE | ID: mdl-38220877

RESUMEN

We present the description of a new genus and new species of Trigonidiidae, Qiongqi crinalis gen. et sp. nov., found in amber deposits at Noije Bum, near Tanai in the Hukawng Valley of northern Myanmar. Its lateralized eyes, short clypeus, exposed mandible, and extremely dense setae entirely covering the body, provides us with new knowledge on the morphology of Trigonidiidae. Additionally, a key to species of fossil Trigonidiidae in amber was provided.


Asunto(s)
Ortópteros , Animales , Ámbar , Mianmar , Fósiles
15.
Zootaxa ; 5383(3): 391-397, 2023 Dec 12.
Artículo en Inglés | MEDLINE | ID: mdl-38221241

RESUMEN

The genus Capillogryllus includes three species, Capillogryllus dolabripalpis Xie & Zheng, 2003, Capillogryllus exilipalpis Xie & Zheng, 2003, Capillogryllus spinae Wu & Ma, 2023. Recently, we found a new species of this genus in Yadong County, Xizang Autonomous Region, and described it here. In addition, we found that the ovipositor varies greatly within this taxon, so we compare and characterize their ovipositor and discuss the feasibility of using the ovipositor alone to identify them. We also provide two keys based on the genitalia of males and females.


Asunto(s)
Gryllidae , Ortópteros , Masculino , Femenino , Animales , Distribución Animal , Tamaño de los Órganos , Estructuras Animales , Tamaño Corporal , China
16.
Zootaxa ; 5353(1): 75-81, 2023 Oct 06.
Artículo en Inglés | MEDLINE | ID: mdl-38221421

RESUMEN

The Mogoplistidae genus Ornebius contains eight species groups and 124 species worldwide, with 14 species recorded from China. Here, we find a new species belonging to the species group alii Bhowmik, 1970 from Yunnan Province, China. The new species, Ornebius exserolimbus sp. nov., is distinct by bearing a protrusion on the anterior margin of the pronotum. This feature was first discovered in scale crickets and it might be related to some special locomotion function of crickets. Besides describing and illustrating the new species, we provide keys for Chinese Ornebius species in this article.


Asunto(s)
Ortópteros , Animales , China , Distribución Animal , Estructuras Animales , Tamaño Corporal , Tamaño de los Órganos
17.
Zootaxa ; 5375(4): 582-588, 2023 Nov 24.
Artículo en Inglés | MEDLINE | ID: mdl-38220801

RESUMEN

Goniogryllus bistriatus Wu & Wang, 1992 was described at the end of the last century, based on the female holotype, and there have been no further studies or reports related to this species since then. Callogryllus yunnanus Wu & Zheng, 1992 also has a similar situation. Although the holotype is a male, its external genitalia have been lost. These issues pose obstacles to subsequent taxonomic research. Recently, we have successively discovered these two species in Huize County, Yunnan Province, China. To address the aforementioned taxonomic problems, this article describes the male specimens of the G. bistriatus and redescribes the female; meanwhile, since the original description of C. yunnanus lacked male genital characteristics, and we have also conducted a complete redescription of this species based on the new specimen.


Asunto(s)
Gryllidae , Ortópteros , Masculino , Femenino , Animales , China , Distribución Animal , Estructuras Animales , Tamaño Corporal , Tamaño de los Órganos
18.
World J Gastrointest Oncol ; 15(2): 286-302, 2023 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-36908323

RESUMEN

BACKGROUND: Cancerous inhibitor of protein phosphatase 2A (CIP2A) is a newly discovered oncogene. It is an active cell proliferation regulatory factor that inhibits tumor apoptosis in gastric cancer (GC) cells. CIP2A is functionally related to chemoresistance in various types of tumors according to recent studies. The underlying mechanism, however, is unknown. Further, the primary treatment regimen for GC is oxaliplatin-based chemotherapy. Nonetheless, it often fails due to chemoresistance of GC cells to oxaliplatin. AIM: The goal of this study was to examine CIP2A expression and its association with oxaliplatin resistance in human GC cells. METHODS: Immunohistochemistry was used to examine CIP2A expression in GC tissues and adjacent normal tissues. CIP2A expression in GC cell lines was reduced using small interfering RNA. After confirming the silencing efficiency, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium and flow cytometry assays were used to evaluate cell proliferation and apoptosis caused by oxaliplatin treatment. Further, the key genes and protein changes were verified using real-time quantitative reverse transcription PCR and Western blotting, respectively, before and after intervention. For bioinformatics analysis, we used the R software and Bioconductor project. For statistical analysis, we used GraphPad Prism 6.0 and the Statistical Package for the Social Sciences software version 20.0 (IBM, Armonk, United States). RESULTS: A high level of CIP2A expression was associated with tumor size, T stage, lymph node metastasis, Tumor Node Metastasis stage, and a poor prognosis. Further, CIP2A expression was higher in GC cells than in normal human gastric epithelial cells. Using small interfering RNA against CIP2A, we discovered that CIP2A knockdown inhibited cell proliferation and significantly increased GC cell sensitivity to oxaliplatin. Moreover, CIP2A knockdown enhanced oxaliplatin-induced apoptosis in GC cells. Hence, high CIP2A levels in GC may be a factor in chemoresistance to oxaliplatin. In human GC cells, CIP2A regulated protein kinase B phosphorylation, and chemical inhibition of the protein kinase B signaling pathway was significantly associated with increased sensitivity to oxaliplatin. Therefore, the protein kinase B signaling pathway was correlated with CIP2A-enhanced chemoresistance of human GC cells to oxaliplatin. CONCLUSION: CIP2A expression could be a novel therapeutic strategy for chemoresistance in GC.

19.
Heliyon ; 9(3): e14714, 2023 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-36994412

RESUMEN

Objective: To investigate the value of Hypoxia-inducible factor 1 A (HIF1A) in predicting lymph node metastasis (LNM) stage and clinical outcomes of papillary thyroid cancer (PTC) patients. Materials and methods: The HIF1A gene expression analysis in PTC was performed by bioinformatics approaches followed by evaluating its protein level using immunohistochemistry analysis. The role of HIF1A in predicting the LNM stage was evaluated by logistic regression analysis, nomogram construction, and receiver operating characteristic (ROC) analysis. We performed survival analyses to determine its prognostic value. Enrichment analysis was conducted, and immune cell infiltration and stromal content were evaluated to examine the underlying mechanism of HIF1A in PTC. Results: HIF1A transcription and protein levels were significantly high in PTC tissue (P < 0.05). Its overexpression predicted high LNM risk and unfavorable prognosis for PTC patients (P < 0.05). Cox regression analysis revealed HIF1A as an independent prognostic biomarker for the disease-free interval (DFI) (P < 0.01). In addition, HIF1A was positively related to tumor-suppressive immunity but was negatively correlated with anti-tumor immunity. HIF1A upregulation was also associated with increased stromal content. Conclusions: HIF1A overexpression is an independent predictor for worse DFI in PTC. The HIF1A expression may affect the prognosis of PTC patients through immune- and stroma-related pathways. Our study provides new insight into the role of HIF1A in PTC biology and clinical management.

20.
Zootaxa ; 5361(4): 573-578, 2023 Nov 03.
Artículo en Inglés | MEDLINE | ID: mdl-38220740

RESUMEN

The article describes two new species of Trigonidiidae Saussure, 1874, namely Pteronemobius yuani Ma & Wang sp. nov. and Metiochodes tianfuensis Ma, Yuan & Gu sp. nov. The former belongs to Pteronemobius Jacobson, 1904, and is similar to Pteronemobius gifuensis (Shiraki, 1911) but has a long and narrow epiphallic median lobe, and the outer posterior tibia is armed with three dorsal spurs; while the latter belongs to Metiochodes Chopard, 1932, which is similar to Metiochodes flavescens Chopard, 1932, but the epiphallic median lobe is concave. Here, we describe and illustrate these new species.


Asunto(s)
Gryllidae , Ortópteros , Animales , Estructuras Animales , Distribución Animal , Tamaño Corporal , Tamaño de los Órganos , China
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