Fulvanines J-K, two rare lactam pyrrole alkaloids from Hemerocallis fulva.

Two rare jatropham lactam derivatives, named as fulvanines J-K (1-2), together with six known pyrrole alkaloids, 5,5'-oxydi(3-methyl-3-pyrrolin-2-one) (3), (-)-5-hydroxy-3-methyl-3-pyrrolin-2-one (jatropham) (4), (±)-5-O-methyljatropham (5), perlolyrine (6), butyl-2-formyl-5-(hydroxymethyl)-1H-pyrrole-1-butanoate (7), and hemerocallisamine II (8), were isolated from the flower of Hemerocallis fulva. Their structures were elucidated on the basis of spectroscopic methods and compared with the NMR spectra data in the literature. All compounds were evaluated for their anti-complementary activity in vitro, and compounds 1, 4, and 6 exhibited anti-complement effect with CH50 values from 0.61 to 1.42 mM.

Dynamic assessment of the COVID-19 vaccine acceptance leveraging social media data.

Vaccination is the most effective way to provide long-lasting immunity against viral infection; thus, rapid assessment of vaccine acceptance is a pressing challenge for health authorities. Prior studies have applied survey techniques to investigate vaccine acceptance, but these may be slow and expensive. This study investigates 29 million vaccine-related tweets from August 8, 2020 to April 19, 2021 and proposes a social media-based approach that derives a vaccine acceptance index (VAI) to quantify Twitter users' opinions on COVID-19 vaccination. This index is calculated based on opinion classifications identified with the aid of natural language processing techniques and provides a quantitative metric to indicate the level of vaccine acceptance across different geographic scales in the U.S. The VAI is easily calculated from the number of positive and negative Tweets posted by a specific users and groups of users, it can be compiled for regions such a counties or states to provide geospatial information, and it can be tracked over time to assess changes in vaccine acceptance as related to trends in the media and politics. At the national level, it showed that the VAI moved from negative to positive in 2020 and maintained steady after January 2021. Through exploratory analysis of state- and county-level data, reliable assessments of VAI against subsequent vaccination rates could be made for counties with at least 30 users. The paper discusses information characteristics that enable consistent estimation of VAI. The findings support the use of social media to understand opinions and to offer a timely and cost-effective way to assess vaccine acceptance.

Studies on the biosynthetic pathways of melanin in Auricularia auricula.

Melanin is a natural pigment ubiquitously present in living organisms, including bacteria, fungi, plants, and animals. Melanin produced by the edible mushroom Auricularia auricula has a remarkable potential for resource development. Several A. auricula strains planted across China were collected and analyzed for mycelial growth rate and colony red, green, blue (RGB) value for color block. Further, the effects of various nutrients on melanin formation, including different carbon and nitrogen sources were evaluated to optimize the medium for submerged fermentation. The pathways involved in the biosynthesis of melanin in A. auricula were investigated using an enzyme inhibitor assay and intermediate determination. In addition, the functional activity of purified A. auricula melanin was assessed. The highest melanin yield (1.797 g/L) was displayed by strain AU-3 in medium I. A. auricula melanin was composed of eumelanin, pheomelanin, and 1,8-dihydroxynaphthalene melanin, and the biosynthetic pathways involved were Raper-Mason and 1,8-dihydroxynaphthalene melanin pathway. In addition, melanin purified from A. auricula exhibited substantial antioxidant, antibacterial, and antitumor activities.

Accumulation risk and source apportionment of heavy metals in different types of farmland in a typical farming area of northern China.

The types of land used for farmland can greatly influence the source and accumulation risk of heavy metals in soil. However, the apportioning quantitatively the source of soil heavy metals has been studied insufficiently, especially in terms of different types of farmland. In this study, a total of 252 soil samples were taken from dry land, paddy fields and greenhouse fields in the Jinyuan district of Taiyuan city, China, to assess the accumulation risk of heavy metals (As, Cd, Cr, Cu, Hg, Ni, Pb and Zn). The results were then integrated, and source apportionment was evaluated by geospatial analysis, multivariate statistical analysis and positive matrix factorization (PMF). Cr, Cd and Hg were the dominant pollutants in the studied area. Accumulation risk by Cd and Cu was more severe in greenhouse fields than in dry land or paddy fields, whereas As, Hg and Pb had relatively higher accumulation in paddy fields than in dry land or greenhouse fields. Hg was derived mainly from coal combustion by atmospheric precipitation for the three types of farmland. Long-term irrigation using sewage is the main reason for the accumulation of Cu and Ni in dry land soil, Cu and Zn in paddy field soil and Zn in greenhouse soil. Cd in dry land, Cd and Pb in paddy fields and Cd, Cu, Ni and Pb in greenhouse fields were primarily added to soil through fertilization. Sewage irrigation and fertilization were the dominant sources of heavy metals for paddy field (31.3%) and greenhouse field (33.1%), respectively.

Synthesis and production of the antitumor polyketide aurovertins and structurally related compounds.

Aurovertins belong to a family of highly reducing polyketides sharing a polyene α-pyrone-type structure. These compounds comprise aurovertin, asteltoxin, avertoxin, citreoviridin, verrucosidin, and their derivatives, which exihibit potent antitumor, antiviral, and antibacterial activities. Until now, over 40 aurovertins and structurally related compounds have been found in the fungal kingdom. Due to the unique structural feature and interesting bioactivities, significant progresses have been achieved for the structural identification, chemical synthesis, and biosynthesis of the mentioned compounds. Understanding of aurovertin biosynthetic mechanism provides a solid basis for engineering the metabolic pathway of those compounds by rational design and realizing their production in the model fungal host.

Rational design for heterologous production of aurovertin-type compounds in Aspergillus nidulans.

Aurovertins are the structurally diverse polyketides that distribute widely in different fungal species. They feature a 2,6-dioxabicyclo[3.2.1]-octane ring in structure and exhibit the potential antitumor activity against breast cancer as F1-ATPase ß subunit inhibitor. In this study, we constructed the biosynthetic pathway of aurovertin in an Aspergillus nidulans host and obtained seven aurovertin-type compounds. Surprisingly, three new aurovertin geometric isomers were characterized. By introducing an inducible promoter xylP(p) in the pathway gene acyltransferase aurG, we can control the product ratios among different aurovertin compounds by adding glucose and/or inducer xylose. The yields of aurovertins could be increased up to about 20 times by adding a constitutive promoter gpdA(p) to transcription factor AurF, which indicates AurF's positive role in the biosynthesis of aurovertin. Taken together, our results provided not only an efficient way to generate bioactive fungal natural products but also realized the rational controlling their yields with designed promoters.

[Progress in heterologous expression of fungal natural products-A review].

Fungal products are important resources for natural drugs. Availabilities of many fungal genome sequences indicate that fungi have the huge potential to produce secondary metabolites. However, many valuable fungal natural products are hard to obtain under regular lab conditions because fungi are slow to grow, difficult to genetically modify, or have low yield of desired metabolites. Therefore, developing a heterologous expression system has been an efficient approach to discover novel natural product and elucidate the complicated biosynthetic pathways. This review describes several heterologous expression systems that have been successfully used for the expression of fungal biosynthetic gene clusters. Also, we address the application perspective based on the emerging DNA assembly technologies.

Melanin in Auricularia auricula: biosynthesis, production, physicochemical characterization, biological functions, and applications.

Auricularia auricular (A. auricula), a nutritious fungus and traditional medicinal resource, is known for melanin. This review aims to summarize the research progress on melanin in A. auricula, specifically focusing on biosynthesis, fermentation production, extraction processes, physicochemical characterization, biological functions, and applications. The biosynthesis of melanin in A. auricula primarily involves the oxidative polymerization reaction of phenolic compounds. To enhance melanin production, strategies such as deep fermentation culture, selection of optimal fermentation materials, and optimization of the culture medium have been employed. Various extraction processes have been compared to determine their impact on the physicochemical properties and stability of melanin. Moreover, the antioxidant and antibiofilm activities of A. auricula melanin, as well as its potential beneficial effects on the human body through in vivo experiments, have been investigated. These findings provide valuable insights into the application of A. auricula melanin and serve as a reference for future research in this field.

Prediction of the global occurrence of maize diseases and estimation of yield loss under climate change.

BACKGROUND: Climate change significantly impacts global maize production via yield reduction, posing a threat to global food security. Disease-related crop damage reduces quality and yield and results in economic losses. However, the occurrence of diseases caused by climate change, and thus crop yield loss, has not been given much attention. RESULTS: This study aims to investigate the potential impact of six major diseases on maize yield loss over the next 20 to 80 years under climate change. To this end, the Maximum Entropy model was implemented, based on Coupled Model Intercomparison Project 6 data. The results indicated that temperature and precipitation are identified as primary limiting factors for disease onset. Southern corn rust was projected to be the most severe disease in the future; with a few of the combined occurrence of all the selected diseases covered in this study were predicted to progressively worsen over time. Yield losses caused by diseases varied per continent, with North America facing the highest loss, followed by Asia, South America, Europe, Africa, and Oceania. CONCLUSION: This study provides a basis for regional projections and global control of maize diseases under future climate conditions. © 2024 Society of Chemical Industry.

Preparation of nitrogen doped hyper-crosslinked polymer-based hard carbon for high performance Li+/Na+ battery anode.

Hyper cross-linked polymers (HCPs), as a key precursor of hard carbon (HC) anode materials, stand out because of their capacity for molecular-scale structural design and comparatively straightforward preparation techniques, which are not seen in other porous materials synthesized procedure. A novel synthesis method of HCPs is developed in this paper, which is through the incorporation of functional macromolecules, the structural control and heteroatom doping of the product has been achieved, thus augmenting its electrochemical performance in batteries. In this work, carbonized tetraphenylporphyrin zinc (TPP-Zn) doped HCP-based hard carbon (CTHCP) with stable structure was prepared by Friedel-Crafts reaction and carbonization by using naphthalene and trace TPP-Zn as monomers, dimethoxybenzene (DMB) as crosslinking agent and FeCl3 as catalyst. The introduction of TPP-Zn, a functional macromolecule with unique two-dimensional structure, realized the pore structure regulation and N doping of the raw carbonized HCP-based hard carbon (CHCP). The results showed that CTHCP had higher mesoporous volume, N content and wider layer spacing than CHCP. In addition, CTHCP anode exhibited excellent Li+/Na+ storage performance, initial reversible capacity, rate performance and long cycle life. More amount of N-containing (N-5) active sites and mesoporous content in CTHCP anode was the main reason for the improvement of Na+ storage effect. While the increased interlayer spacing had a greater effect on the lithium storage capacity. This study uncovered the design rules of HC anode materials suitable for Li+/Na+ batteries and provided a new idea for the preparation of high-performance HC anode materials.

Dissolving microneedle patch-assisted transdermal delivery of methotrexate improve the therapeutic efficacy of rheumatoid arthritis.

Methotrexate (MTX) is a first-line treatment for rheumatoid arthritis (RA), but its clinical use is greatly limited by the adverse effects and poor patient compliance caused by traditional oral administration or injection. In recent years, some transdermal drug delivery systems have received considerable attention due to overcoming these shortcomings. In this study, we developed dissolving microneedle patch (DMNP) for transdermal delivery of MTX to treat RA safely and effectively. The morphology, mechanical strength, skin insertion, drug content, in vitro transdermal delivery, and other properties of DMNP were characterized. Meanwhile, the adjuvant-induced arthritis model of rats was established to investigate the therapeutic effect of MTX-loaded DMNP in vivo. The results showed that the microneedles had excellent morphology with neat array and complete needles, good puncture performance and mechanical strength, and rapid intradermal dissolution rate. In vitro transdermal delivery results indicated that microneedles could significantly increase drug transdermal permeation compared with the cream group. The pharmacological study showed that MTX-loaded DMNP significantly alleviated paw swelling, inhibit inflammatory response via downregulating the levels of TNF-α and IL-1ß, relieved synovium destruction with less cartilage erosion, and slowed the progression of RA in AIA rats. Besides, DMNP presented better therapeutic performance than cream or intragastric administration at the same dosage of MTX. In conclusion, the MTX-loaded dissolving microneedle patch has advantages of safety, convenience, and high efficacy over conventional administrations, laying a foundation for the transdermal drug delivery system treatment of rheumatoid arthritis.

The phylogenetic and phylogeographic landscape of the beak and feather disease virus, 1996-2022.

The beak and feather disease virus (BFDV), causative agent of Psittacine beak and feather disease (PBFD), is a highly fatal and widespread virus that infects both the wild and captive Psittaciformes around the world. The BFDV genome is a ssDNA of approximately 2 kb in size, making it among the smallest known pathogenic viruses. Though, the virus is placed in Circoviridae family of the Circovirus genus, there is no classification system on clade and sub-clade level according to the International Committee on Taxonomy of Viruses and the strains are grouped on the bases of geographic locations. Thus, we provide the latest and robust phylogenetic classification of BFDVs in this study based on full-length genomic sequences, grouping all the available 454 strains detected during 1996-2022 into two distinct clades, e.g., GI and GII. The GI clade is further divided into six sub-clades (GI a-f), while GII into two sub-clades (GII a and b). In addition, the phylogeographic network identified high variability among the BFDV strains, showing several branches, where all the branches are connected to four strains, e.g., BFDV-ZA-PGM-70A(GenBank ID: HM748921.1, 2008-South Africa), BFDV-ZA-PGM-81A(GenBank ID: JX221009.1, 2008-South Africa), BFDV14(GenBank ID: GU015021.1, 2010-Thailand) and BFDV-isolate-9IT11(GenBank ID: KF723390.1, 2014-Italy). Furthermore, we identified 27 recombination events in the rep (replication-associated protein) and cap (capsid protein) coding regions using the complete genomes of BFDVs. Similarly, the amino acids variability analysis indicated that both the rep and cap regions are highly variable with values exceeding the variability coefficient estimation limit of 1.00, speculating the possible amino acids drift with the emergence of new strains. The findings provided in this study may offer the latest phylogenetic, phylogeographic and evolutionary landscape of the BFDVs.

Quantitative characterization of filamentous fungal promoters on a single-cell resolution to discover cryptic natural products.

Characterization of filamentous fungal regulatory elements remains challenging because of time-consuming transformation technologies and limited quantitative methods. Here we established a method for quantitative assessment of filamentous fungal promoters based on flow cytometry detection of the superfolder green fluorescent protein at single-cell resolution. Using this quantitative method, we acquired a library of 93 native promoter elements from Aspergillus nidulans in a high-throughput format. The strengths of identified promoters covered a 37-fold range by flow cytometry. PzipA and PsltA were identified as the strongest promoters, which were 2.9- and 1.5-fold higher than that of the commonly used constitutive promoter PgpdA. Thus, we applied PzipA and PsltA to activate the silent nonribosomal peptide synthetase gene Afpes1 from Aspergillus fumigatus in its native host and the heterologous host A. nidulans. The metabolic products of Afpes1 were identified as new cyclic tetrapeptide derivatives, namely, fumiganins A and B. Our method provides an innovative strategy for natural product discovery in fungi.

Phylogeographic dynamics and molecular characteristics of Enterovirus 71 in China.

Enterovirus 71 (EV71) and coxsackievirus (CV-A16) are the major etiological agents of hand, foot and mouth disease (HFMD). This report reviewed the full-length genomic sequences of EV71 identified in different provinces of China between 1998 and 2019 (a total of 312) in addition to eight worldwide reference genomes to address the genomic evolution and genetic events. The main prevalent EV71 strians in China are C4 genotypes, co-circulating with a few A, B5, C1, and C2 subgenotypes. A new emerging subgenotype in China was identified and classified as B6 genotype. Phylogeographic analysis revealed multiple branches, where a Jiangsu strain 2006-52-9 (GenBank ID: KP266579.1) was linked to different subgenotypes through multiple long mutant branches, including the CV-A16 viruses through the A genotype. Furthermore, identification of 28 natural recombination events suggests that the emergence of new genotypes are associated with intratypic recombination involving EV71 strains and intertypic recombination between EV71 and CV-A16 strains. Compared with the structural proteins, the non-structural proteins of EV71 seem to be highly variable with the highest variable regions of peptidase C3 (3C protein), P2A, and the N-terminus of RNA-dependent RNA polymerase. This study updates the phylogenetic and phylogeographic information of EV71 and provides clues to the emergence of new genotypes of EV71 based on genetics.

How has airport service quality changed in the context of COVID-19: A data-driven crowdsourcing approach based on sentiment analysis.

Airport service quality (ASQ) is a competitive advantage for airport management in today's airport market. Since the COVID-19 health crisis has unprecedentedly influenced airport regulations and operations, effective measurement of ASQ has become crucial for airport administrations. Surveying travelers' attitudes is useful for ASQ assessment but collecting responses could be time-consuming and costly. Therefore, this paper adopts a data-driven crowdsourcing approach to study ASQ during the COVID-19 pandemic by investigating Google Maps reviews from the 98 busiest U.S. airports. To do so, this study develops a topical ontology of keywords regarding ASQ attributes and uses a sentiment tool to derive passengers' attitudes. Through sentiment analysis, Google Maps reviews show more positive sentiment toward environment and personnel but remain constant about facilities during COVID-19. The lexical salience-valence analysis (LSVA) is then applied to explain such changes by tracking the sentiment of frequent words in reviews. Through correlation and regression analysis, this study demonstrates that rating is significantly related to check-in, environment, and personnel in pre-and post-COVID periods. Additionally, the effect of access, wayfinding, facilities, and environment on rating significantly differs between the two periods. The findings illustrate the effectiveness of leveraging online reviews and offer practical implications for what matters to air travelers, especially in the COVID-19 context.

Non-negligible Influence of Oxygen in Hard Carbon as an Anode Material for Potassium-Ion Batteries.

The development of potassium-ion batteries (PIBs) has been impeded by the lack of an appropriate carbon anode material that could accommodate K+ with a large ionic radius. Hard carbon with low cost and larger interlayer spacing is a promising anode material for PIBs. However, the impact of oxygen-containing functional groups in hard carbon (HC) is less reported. Herein, a hypercrosslinked polymer (HCLP) is prepared and used for the synthesis of microporous hard carbons with superior structural stability and abundant oxygen-containing functional groups and defects, in which the crosslinking agent provided copious oxygen atoms. It is found that a large number of C═O groups and micropores provide more storage sites for K+. The surface-controlled process is dominated by the reversible reaction of C═O + K+ + e- ↔ C-O-K, which directly increases the capacity contribution. The HCs obtained at 600 °C exhibit good cycling and rate performance with an initial specific capacity of about 254.3 mAh g-1 and the capacity retention of 83.2% after 200 cycles at 50 mA g-1. The capacity reached up to 121 mAh g-1 at 2 A g-1. A possible capacitive-adsorption mechanism is proposed by kinetic analysis. The redox reaction mechanism between C═O and K+ at the HC is clearly also revealed.

Identification of novel recombinants and proposed standard reference genomes for phylogenetic classification of canine parvovirus-2 (CPV-2): Comprehensive analysis revealing global evolutionary trait.

Sustained spread and continuous evolution of CPV-2 generate new genetic information; nevertheless, there is no adopted phylogenetic tool, and parvo virologists still refer to the three antigenic variants. Herein, this report attempted to review the evolutionary trait of CPV-2 and proposed standard reference genomes using the Maximum Likelihood-based phylogenetic analysis and Parsimony-Informative Sites. The analysis revealed three main evolutionary pathways where CPV-2 strains cluster into distinct clades depicted as GI, GII, or GIII, respectively. Furthermore, novel CPV-2 natural recombinants were detected, occurring only between the newly identified strains (2017-2020). Those findings provide unique insights into the evolutionary relatedness of CPV-2, clarify discrepancies between different geographic areas and will contribute to achieving a more reliable CPV-2 genetic and evolutionary genotyping classification.

The Role of Hydroxycinnamic Acid Amide Pathway in Plant Immunity.

The compounds involved in the hydroxycinnamic acid amide (HCAA) pathway are an important class of metabolites in plants. Extensive studies have reported that a variety of plant hydroxycinnamamides exhibit pivotal roles in plant-pathogen interactions, such as p-coumaroylagmatine and ferulic acid. The aim of this review is to discuss the emerging findings on the functions of hydroxycinnamic acid amides (HCAAs) accumulation associated with plant defenses against plant pathologies, antimicrobial activity of HCAAs, and the mechanism of HCAAs involved in plant immune responses (such as reactive oxygen species (ROS), cell wall response, plant defense hormones, and stomatal immunity). However, these advances have also revealed the complexity of HCAAs participation in plant defense reactions, and many mysteries remain to be revealed. This review provides an overview of the mechanistic and conceptual insights obtained so far and highlights areas for future exploration of phytochemical defense metabolites.

Can social media data be used to evaluate the risk of human interactions during the COVID-19 pandemic?

The U.S. has taken multiple measures to contain the spread of COVID-19, including the implementation of lockdown orders and social distancing practices. Evaluating social distancing is critical since it reflects the risk of close human interactions. While questionnaire surveys or mobility data-based systems have provided valuable insights, social media data can contribute as an additional instrument to help monitor the risk of human interactions during the pandemic. For this reason, this study introduced a social media-based approach that quantifies the pro/anti-lockdown ratio as an indicator of the risk of human interactions. With the aid of natural language processing and machine learning techniques, this study classified the lockdown-related tweets and quantified the pro/anti-lockdown ratio for each state over time. The anti-lockdown ratio showed a moderate and negative correlation with the state-level social distancing index on a weekly basis, suggesting that people are more likely to travel out of the state where the higher anti-lockdown level is observed. The study further showed that the perception expressed on social media could reflect people's behaviors. The findings of the study are of significance for government agencies to assess the risk of close human interactions and to evaluate their policy effectiveness in the context of social distancing and lockdown.

Anti-osteoporosis medication treatment pattern after osteoporotic fracture during 2010-2016 in Fujian, China.

This study demonstrates a low anti-osteoporosis drug treatment rate (22.1% in women, 9.5% in men) after osteoporotic fracture in the real-world setting of Fujian, China. The primary medication was calcitonin. The suboptimal treatment was particularly critical among men, low-level hospitals, wrist/vertebral fracture, and the younger elderly patients. INTRODUCTION: The objective of this study was to investigate the prescription patterns and related influencing factors of anti-osteoporosis drug prescribing after osteoporotic fracture in Fujian, China, between 2010 and 2016. METHODS: This is a retrospective cohort study based on an existing electronic health record database (National Healthcare Big Data in Fuzhou, China, 37 hospitals included). Patients over 50 years old with newly diagnosed osteoporotic fractures between 2010 and 2016 were included. Postfracture osteoporosis therapies were summarized by overall and fracture site. Multivariate logistic regression was performed to identify influencing factors of anti-osteoporosis medication (AOM) prescription. RESULTS: Overall, 22.1% of women and 9.5% of men over 50 years old received AOM treatment after osteoporotic fracture within 1 year during 2010-2016, with particular low use of bisphosphonates, 5.3% in women and 1.5% in men. The highest rate of AOM treatment was found in patients with hip fracture (24.5%), followed by vertebral fracture (14.2%) and wrist fracture (2.3%). Of the AOM-treated patients, 90.5% received calcitonin therapy. The treatment rate of AOM showed a slight decline during 2010-2016, but steady rise trends were observed in Ca/vitamin D (VD) prescription. Hospital level, age, sex, previous osteoporosis, previous AOM prescription, and previous oral glucocorticoid prescription were strong predicting factors of AOM prescription. CONCLUSION: In a real-world setting, AOM treatment was suboptimal and the treatment rate even decreased over time among osteoporosis fracture patients in Fujian, China. The suboptimal treatment was particularly critical among men, low-level hospitals, wrist/vertebral fracture, and the younger elderly patients.