RESUMEN
INTRODUCTION: In recent years, with widespread laparoscopic cholecystectomy and liver transplantation, complications involving the biliary system are increasing. All current techniques have a high risk of recurrence or high-morbidity. MATERIAL AND METHODS: A 3-dimensional collagen bile duct modified with agarose hydrogel was developed to substitute the affected extrahepatic bile duct. It was used in 40 guinea pigs and the histology and physiology was studied at 4 weeks, 3 and 6 months after transplantation. CONCLUSIONS: The graft shows to have a high potential in applications to treat hepatobiliary diseases which require surgery.
Asunto(s)
Conductos Biliares Extrahepáticos/cirugía , Colágeno , Prótesis e Implantes , Animales , Procedimientos Quirúrgicos del Sistema Biliar/métodos , Materiales Biocompatibles , Cobayas , Diseño de PrótesisRESUMEN
Introducción En la actualidad el tratamiento de la vía biliar extrahepática afectada por cáncer, estenosis, lesiones iatrogénicas y otras patologías consiste en la resección de las mismas, y una anastomosis en los casos más graves de la placa hiliar biliar al intestino delgado. Material y métodos Nuestro grupo ha desarrollado un tubo tridimensional de colágeno y agarosa para reparar la vía biliar. Se emplearon 40 animales de experimentación, que fueron estudiados fisiológica e histológicamente a las 4 semanas, 3 y 6 meses. Conclusiones Nuestras prótesis mostraron gran histocompatibilidad y mantenían una normofisiología que hace pensar en las posibles aplicaciones clínicas que podrían tener en un futuro (AU)
Introduction In recent years, with widespread laparoscopic cholecystectomy and liver transplantation, complications involving the biliary system are increasing. All current techniques have a high risk of recurrence or high-morbidity. Material and methods A 3-dimensional collagen bile duct modified with agarose hydrogel was developed to substitute the affected extrahepatic bile duct. It was used in 40 guinea pigs and the histology and physiology was studied at 4 weeks, 3 and 6 months after transplantation. Conclusions The graft shows to have a high potential in applications to treat hepatobiliary diseases which require surgery (AU)