RESUMEN
The human proteome comprises of all of the proteins produced by the sequences translated from the human genome with additional modifications in both sequence and function caused by nonsynonymous variants and posttranslational modifications including cleavage of the initial transcript into smaller peptides and polypeptides. The UniProtKB database (www.uniprot.org) is the world's leading high-quality, comprehensive and freely accessible resource of protein sequence and functional information and presents a summary of experimentally verified, or computationally predicted, functional information added by our expert biocuration team for each protein in the proteome. Researchers in the field of mass spectrometry-based proteomics both consume and add to the body of data available in UniProtKB, and this review highlights the information we provide to this community and the knowledge we in turn obtain from groups via deposition of large-scale datasets in public domain databases.
Asunto(s)
Proteoma , Proteómica , Humanos , Proteoma/genética , Bases de Datos de Proteínas , Secuencia de Aminoácidos , PéptidosRESUMEN
SWISS-PROT, a curated protein sequence data bank, contains not only sequence data but also annotation relevant to a particular sequence. The annotation added to each entry is done by a team of biologists and comes, primarily, from articles in journals reporting the actual sequencing and sometimes characterisation. Review articles and collaboration with external experts also play a role along with the use of secondary databases like PROSITE and Pfam in addition to a variety of feature prediction methods. Annotation added by these methods is checked for relevance and likelihood to a particular sequence. The onset of genome sequencing has led to a dramatic increase in sequence data to be included in SWISS-PROT. This has led to the production of TrEMBL (Translation of the EMBL database). TrEMBL consists of entries in a SWISS-PROT format that are derived from the translation of all coding sequences in the EMBL nucleotide sequence database, that are not in SWISS-PROT. Unlike SWISS-PROT entries those in TrEMBL are awaiting manual annotation. However, rather than just representing basic sequence and source information, steps have been taken to add features and annotation automatically. In taking these steps it is hoped that TrEMBL entries are enhanced with some indication as to what a protein is, could or may be.
Asunto(s)
Bases de Datos Factuales , Genoma , Proteínas/genética , Secuencia de Aminoácidos , Animales , Biotecnología , Humanos , Datos de Secuencia Molecular , Investigación , Alineación de SecuenciaAsunto(s)
Estimulantes del Sistema Nervioso Central/efectos adversos , Trastornos Relacionados con Cocaína , Alucinógenos/efectos adversos , Metanfetamina/efectos adversos , N-Metil-3,4-metilenodioxianfetamina/efectos adversos , Adolescente , Adulto , Trastornos Relacionados con Cocaína/prevención & control , Trastornos Relacionados con Cocaína/terapia , Humanos , Trastornos Relacionados con Sustancias/prevención & control , Trastornos Relacionados con Sustancias/terapiaAsunto(s)
Hidroxibutiratos/efectos adversos , Drogas Ilícitas/efectos adversos , Trastornos Relacionados con Sustancias/terapia , Adolescente , Adulto , Humanos , Centros de Tratamiento de Abuso de Sustancias , Trastornos Relacionados con Sustancias/complicaciones , Trastornos Relacionados con Sustancias/epidemiologíaRESUMEN
No disponible
Asunto(s)
Humanos , Trastornos Relacionados con Cocaína/prevención & control , Atención Primaria de Salud , Servicios Preventivos de SaludRESUMEN
No disponible
No disponible
Asunto(s)
Humanos , Adolescente , Adulto , Estimulantes del Sistema Nervioso Central/efectos adversos , Trastornos Relacionados con Cocaína/prevención & control , Trastornos Relacionados con Cocaína/terapia , Alucinógenos/efectos adversos , Metanfetamina/efectos adversos , N-Metil-3,4-metilenodioxianfetamina/efectos adversos , Trastornos Relacionados con Sustancias/prevención & control , Trastornos Relacionados con Sustancias/terapiaRESUMEN
No disponible