RESUMEN
Rotavirus is the most common cause of severe diarrhea in infants and young children world-wide, with the highest fatality rate in developing countries. We investigated the presence and seasonal distribution of group A rotavirus infection among Thai children. The data will be used for vaccine development. Samples were collected from infants and children with acute gastroenteritis or diarrhea admitted to two hospitals between June 2009 and May 2011. Group A rotaviruses were detected in 250 (44.5%) of 562 specimens by RT-PCR. The most prevalent genotype was G3P[8] (60.4%) followed by G1P[8] (39.2%) and G2P[4] (0.4%). The specimens were subjected to phylogenetic analysis based on the VP7 and VP4 genes. We examined the rotavirus genotypes and compared them with data from the GenBank database.
Asunto(s)
Diarrea/epidemiología , Diarrea/virología , Infecciones por Rotavirus/epidemiología , Rotavirus/genética , Secuencia de Bases , Preescolar , Heces/virología , Genes Virales , Genotipo , Humanos , Lactante , Recién Nacido , Datos de Secuencia Molecular , Prevalencia , ARN Viral , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Rotavirus/clasificación , Análisis de Secuencia de ARN , Tailandia/epidemiologíaRESUMEN
BACKGROUND: We performed phylogenetic and sequence analysis by Basic Local Alignment Search Tool (BLAST) of a complete Human Rotavirus (HRV) genome isolated from a hospitalized child with acute gastroenteritis in Thailand. FINDINGS: The results indicated an uncommon strain characterized by multiple re-assortments in the VP3, VP4, VP6, NSP1, NSP4 and NSP5 genes. The uncommon strain is genotype G3-P[9]-I3-R3-C3-M3-A3-N3-T3-E3-H6, which displays aspects of the AU-1, FRV-1 and corresponds to the feline/canine prototype G3P[9] strain. CONCLUSIONS: The results suggested that nearly all the eleven gene segments of G3P[9] RVA strain CU365 might have originated from feline/canine RVAs (Rotavirus A).