Biocompatible zinc gallogermanate persistent luminescent nanoparticles for fast tumor drainage lymph node imaging in vivo.

Tumor drainage lymph node identification and dissection are crucial for the oncological surgery to prevent/delay the recurrence. However, commercial imaging reagents distinguish the lymph nodes by staining them dark, which would be seriously interfered by blood and surrounding tissues. In this study, we reported the Cr3+/Pr3+-doped zinc gallogermanate persistent luminescent nanoparticles (PLNPs) for fast tumor drainage lymph node imaging with high contrast. PLNPs were synthesized by citrate sol-gel method and dispersed in Tween 80 for in vivo applications. PLNPs were well dispersed in water with hydrodynamic radii of 5 nm and emitted strong persistent luminescence at 696 nm upon the irradiation of UV light. The advantage of afterglow imaging over fluorescent imaging of PLNPs was first established after subcutaneous injection to mice with much higher contrast and less interference of autofluorescence. PLNPs quickly migrated to sentinel lymph nodes after the interdermal injection to extremity of mice. The tumor drainage lymph node imaging was achieved within 5 min upon the intratumoral injection to H460 tumor bearing mice and the signal to noise ratio was 462. Due to the lack of targeting moieties, the intravenous injected PLNPs mainly accumulated in liver. There were no statistical changes in serum biochemistry and abnormal histopathological characteristic, indicating the low toxicity of PLNPs. These findings highlighted the great potential of PLNPs as high-performance imaging reagent for lymph node identification.

Toxicity and photosynthetic inhibition of metal-organic framework MOF-199 to pea seedlings.

Metal-organic framework (MOF) materials are star materials with unique structures and properties. To ensure safe production and applications, the toxicity and environmental hazards of MOF materials should be thoroughly investigated. However, the environmental impact of MOF materials on plants is completely unknown. Herein, we reported the toxicity and photosynthetic inhibitory properties of MOF-199 to pea plants (Pisum sativum L.). MOF-199 was synthesized by hydrothermal method. MOF-199 was copper containing double-pyramid of high surface area (668 m2/g). MOF-199 accelerated the germination of pea seeds, but the total germination rates were unchanged. MOF-199 inhibited the seedling growth at high concentrations. The net photosynthetic rate increased, while the total photosynthesis capability decreased. Damage to the acceptor side of photosystem II was evidenced by chlorophyll fluorescence. Mechanistically, MOF-199 released Cu2+ in the nutrient solution, led to Cu2+ accumulations in seedlings, and promoted oxidative stress. In addition, the photosynthetic inhibitions of MOF-199 were stronger than equivalent concentrations of Cu(NO3)2, implying that MOF-199 particles also contributed to the environmental hazards. Our results highlighted the potential threat of MOF materials to plant growth and photosynthesis.

Fast Identification and Quantification of Graphene Oxide in Aqueous Environment by Raman Spectroscopy.

Today, graphene nanomaterials are produced on a large-scale and applied in various areas. The toxicity and hazards of graphene materials have aroused great concerns, in which the detection and quantification of graphene are essential for environmental risk evaluations. In this study, we developed a fast identification and quantification method for graphene oxide (GO) in aqueous environments using Raman spectroscopy. GO was chemically reduced by hydrazine hydrate to form partially reduced GO (PRGO), where the fluorescence from GO was largely reduced, and the Raman signals (G band and D band) were dominating. According to the Raman characteristics, GO was easily be distinguished from other carbon nanomaterials in aqueous environments, such as carbon nanotubes, fullerene and carbon nanoparticles. The GO concentration was quantified in the range of 0.001-0.6 mg/mL with good linearity. Using our technique, we did not find any GO in local water samples. The transport of GO dispersion in quartz sands was successfully quantified. Our results indicated that GO was conveniently quantified by Raman spectroscopy after partial reduction. The potential applications of our technique in the environmental risk evaluations of graphene materials are discussed further.