RESUMEN
Glioblastoma (GBM) is the most common glioma in adults, with incidence increasing by 3% per year. According to the World Health Organization Classification of Central Nervous System Tumors, GBM is considered a grade IV tumor due to its malignant behavior. The aim of this review is to summarize the main biological aspects of GBM. In particular, we focused our attention on those alterations which have been proven to have an impact on patients' outcome, mainly in terms of overall survival (OS), or on the tumor response to therapies. We have also analyzed the cellular biology and the interactions between GBM and the surrounding environment.
Asunto(s)
Biomarcadores de Tumor/genética , Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/genética , Manejo de la Enfermedad , Glioblastoma/diagnóstico , Glioblastoma/genética , Biomarcadores de Tumor/sangre , Neoplasias Encefálicas/terapia , Receptores ErbB/sangre , Receptores ErbB/genética , Glioblastoma/terapia , Humanos , Proteínas Proto-Oncogénicas B-raf/sangre , Proteínas Proto-Oncogénicas B-raf/genéticaRESUMEN
Crystals of human plasma retinol-binding protein have been obtained from 4.5 M-NaCl buffered at pH 6.8 with 20 mM-cacodylate. The crystals are trigonal with space group R3 and unit cell dimensions, referred to the hexagonal system, a = b = 104.2 A and c = 74.5 A. The crystals diffract to a resolution of 2.0 A.
Asunto(s)
Proteínas de Unión al Retinol , Cristalización , Humanos , Proteínas Plasmáticas de Unión al Retinol , Difracción de Rayos XRESUMEN
Chicken liver basic fatty acid-binding protein (pI = 9.0) has been purified with a high yield by a modification of a method originally applied to rat liver. The final product is highly homogeneous and can be used to grow crystals that belong to two different space groups. The crystals are either tetragonal, space group P4(2)2(1)2 with a = b = 60.2 A and c = 138.1 A or orthorhombic, space group P2(1)2(1)2(1) with a = 60.7 A, b = 40.1 A and c = 66.7 A. The second form appears to be more suitable for X-ray diffraction studies, it diffracts to at least 2.8 A resolution and it is believed to contain one protein molecule in the crystallographic asymmetric unit.
Asunto(s)
Proteínas Portadoras/aislamiento & purificación , Hígado/análisis , Proteínas de Neoplasias , Proteínas del Tejido Nervioso , Aminoácidos/análisis , Animales , Pollos , Cristalografía , Proteína de Unión a los Ácidos Grasos 7 , Proteínas de Unión a Ácidos Grasos , Punto Isoeléctrico , Peso Molecular , Difracción de Rayos XRESUMEN
This is a retrospective study of patients with first attack of myocardial infarction admitted to Medical College Hospital, Calicut during the years 1969 to 1988. The data analysed were compared to other hospital based studies in India and abroad. A striking increase in the percentage of acute myocardial infarction was observed. There was also an increase in the occurrence of myocardial infarction in the young. A properly designed population based study is warranted.
Asunto(s)
Unidades de Cuidados Coronarios/estadística & datos numéricos , Hospitales de Enseñanza/estadística & datos numéricos , Infarto del Miocardio/epidemiología , Adulto , Factores de Edad , Anciano , Femenino , Registros de Hospitales/estadística & datos numéricos , Humanos , Incidencia , India/epidemiología , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Factores Sexuales , Factores de TiempoRESUMEN
The three-dimensional structure of chicken liver basic fatty acid-binding protein has been determined at 2.7 A resolution by X-ray crystallography. Phases were calculated using the multiple isomorphous replacement procedure and a preliminary model was built. This model, with an initial R-factor of 0.57, was then improved by a cycle of refinement by simulated annealing which brought the R factor down to 0.32. The protein is structured as a compact 10-stranded-beta-barrel which encapsulates a residual electron density that can be interpreted as a fatty acid molecule. The NH2-terminus portion of the molecule contains two short alpha-helices. The structure of this liver protein appears very similar to that of the Escherichia coli derived rat intestinal FABP recently determined by X-ray diffraction methods.