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1.
J Esthet Restor Dent ; 36(4): 620-631, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-37950565

RESUMEN

OBJECTIVE: Obtaining strong resin bonds to computer-aided design and computer-aided manufacturing (CAD-CAM) resin-based composites with dispersed fillers (CCRBCs) poses a challenge. Therefore, this study aimed to assess the effect of three universal adhesives and a two-component silane coupling agent on the shear bond strength to three (CCRBCs). MATERIALS AND METHODS: Eight hundred and sixty-four specimens of Brilliant Crios, Lava Ultimate, and Tetric CAD were polished or grit blasted, bonded with Adhese Universal DC, One Coat 7 Universal (OC7), and Scotchbond Universal Plus Adhesive (SBU) with or without a silane primer. Shear bond strength was measured after 24 h and 10,000 thermocycles. Linear regressions were performed (α = 0.05). RESULTS: After thermocycling, bond strengths were similar for the universal adhesives on polished Brilliant Crios and Lava Ultimate (p ≥ 0.408). Grit blasted Tetric CAD showed no significant differences (p ≥ 0.096). The silane primer had minimal impact on grit blasted Tetric CAD (p ≥ 0.384). The silane primer increased the bond strength of OC7 to Brilliant Crios (p = 0.001) but decreased the adhesion of SBU to Brilliant Crios and Lava Ultimate (p ≤ 0.018). CONCLUSIONS: Bond strength of universal adhesives varied with CCRBC type. The two-component silane coupling agent showed mixed effects on adhesive performance. CLINICAL SIGNIFICANCE: Selecting universal adhesives from the same CCRBC product line does not always guarantee superior bond strength. The efficacy of silane coupling agents differs based on the bonding substrate.


Asunto(s)
Recubrimiento Dental Adhesivo , Silanos , Silanos/química , Cementos Dentales , Propiedades de Superficie , Materiales Dentales , Cementos de Resina/química , Diseño Asistido por Computadora , Ensayo de Materiales
2.
J Mater Sci Mater Med ; 33(4): 38, 2022 Apr 11.
Artículo en Inglés | MEDLINE | ID: mdl-35404019

RESUMEN

Zeolite can impart antibacterial properties to dental materials in the long-term when incorporated with inorganic cations. However, due to its porosity, it may jeopardize the mechanical integrity of the dental material. The aim of this project was to determine the effect on physical properties when zeolite is added to commercially available Ag-reinforced Glass Ionomer Cement (GIC). Sample groups were prepared according to the percentage of zeolite-clinoptilolite (0% - control, 0.5%, 1%, 2%, and 4% wt) added to Ag-GIC. Water sorption, solubility, Vickers hardness, and flexural strength were determined. Specifically, 10 × 2 mm circular disks were fabricated for the Vickers hardness, water sorption, and water solubility tests and 25 × 5 × 2 mm bars were created for the flexural strength test. The results from the surface hardness, water sorption, and flexural strength tests suggested that adding 0.5-4% wt of zeolite to Ag-reinforced GIC did not diminish its physical properties. However, the water solubility results showed that higher concentrations (2-4% wt) of zeolite had a statistically significant increase in water solubility compared to the control. Up to 4% wt zeolite can be incorporated into Ag-reinforced GIC without compromising mechanical properties. Incorporation of 0.5-1% wt zeolite to Ag-reinforced GIC will maintain an adequate surface hardness, water sorption, and flexural strength without compromising water solubility. Further research is needed to determine the effects of higher water solubility on clinical efficacy of zeolite modified Ag-GIC. Graphical abstract.


Asunto(s)
Cementos de Ionómero Vítreo , Zeolitas , Ensayo de Materiales , Plata , Propiedades de Superficie , Agua
3.
J Esthet Restor Dent ; 34(1): 136-144, 2022 01.
Artículo en Inglés | MEDLINE | ID: mdl-35083844

RESUMEN

OBJECTIVE: Bi-layer zirconia-based posterior fixed dental prostheses (FDPs) have reportedly a high incidence of veneering ceramic fractures. The CADon technique employs zirconia frameworks veneered with milled lithium disilicate glass-ceramic to overcome these shortcomings but long-term clinical studies are missing. This study evaluated the clinical efficacy of posterior 3-unit CADOn FDPs over a 5-year period. MATERIALS AND METHODS: A total of 25 patients, 16 male and 9 female with a mean age of 55.6 years, received a total of 25 posterior three-unit CADOn FDPs. Patients were recalled for baseline and for follow-up visits at 6, 12, 24, and 60 months. Parameters evaluated were fracture, marginal adaptation, marginal discoloration, wear, polish, color match, surface staining, and retention of these restorations as well as secondary caries of the abutment teeth. RESULTS: A total of 21 FDPs evaluated at 60-months revealed a 100% survival rate. All evaluated clinical parameters were satisfactory (alpha or bravo) and there was no statistically significant difference at different time points, except for surface staining, which was also worse at 60 months compared to baseline (p  ≤  0.001). CONCLUSIONS: CADOn three-unit posterior FDPs demonstrate excellent fracture resistance after 5 years of clinical function. All evaluated clinical parameters were satisfactory, except for surface stain, which also increased over time. CLINICAL SIGNIFICANCE: Conventionally veneered bi-layer zirconia-based posterior FDPs have limited clinical success. Based on this study, alternative methods such as zirconia frameworks veneered with milled lithium disilicate glass-ceramic provide a viable alternative with excellent long-term clinical outcomes.


Asunto(s)
Coronas con Frente Estético , Dentadura Parcial Fija , Cerámica , Materiales Dentales , Porcelana Dental , Fracaso de la Restauración Dental , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Circonio
4.
Molecules ; 27(24)2022 Dec 13.
Artículo en Inglés | MEDLINE | ID: mdl-36557980

RESUMEN

Polymethyl methacrylate (PMMA), an acrylic resin used in orthodontic appliances and removable dentures for its biocompatibility and esthetics, may harbor bacteria on its surface. The present study investigated a new PMMA formula with Gantrez: an antibacterial copolymer of methyl vinyl ether and maleic acid (PVM/MA). Samples were tested for mechanical properties (surface hardness, flexural strength, water sorption, and water solubility) and effects against Streptococcus mutans. Six groups (0%-control, 5%, 10%, 15%, 20%, and 25% Gantrez) of n = 12 were fabricated for physical property tests and analyzed with one-way ANOVA and Prism 6. From these results, three groups (0%, 5%, and 10% Gantrez) were selected for antibacterial tests, and data were analyzed with one-way ANOVA and Tukey's multiple comparison test. Adding 5% and 10% Gantrez into PMMA significantly decreased S. mutans adhesion. There was no significant difference between the control vs. 5%, 10%, 15%, and 20% Gantrez (p > 0.05) for surface hardness, the control vs. 5% Gantrez (p > 0.05) for flexural strength, and the control vs. 5 and 10% Gantrez for water sorption and water solubility. Overall, incorporating 5% Gantrez into PMMA may be a promising solution to reduce bacterial adhesion without changing the acrylic resin's physical properties.


Asunto(s)
Antiinfecciosos , Polimetil Metacrilato , Resinas Acrílicas , Propiedades de Superficie , Antibacterianos/farmacología , Agua , Ensayo de Materiales
5.
J Prosthodont ; 31(S1): 30-37, 2022 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-35313026

RESUMEN

PURPOSE: This in vitro study evaluated the dimensional accuracy of three 3D printers and one milling machine with their respective polymeric materials using a simplified geometrical model. MATERIALS AND METHODS: A simplified computer-aided design (CAD) model was created. The test samples were fabricated with three 3D printers: a dental desktop stereolithography (SLA) printer, an industrial SLA printer, and an industrial fused deposition modeling (FDM) printer, as well as a 5-axis milling machine. One polymer material was used per industrial printer and milling machine while two materials were used with the dental printer for a total of five study groups. Test specimens were then digitized using a laboratory scanner. The virtual outer caliper method was used to measure the linear dimensions of the digitized 3D printed and milled specimens in x-, y-, and z-axes, and compare them to the known values of the CAD model. Data were analyzed with Kruskal-Wallis one-way ANOVA on Ranks followed by the Tukey's test. RESULTS: Milled specimens were not significantly different from the CAD model in any dimension (p > 0.05). All 3D printed specimens were significantly different from the CAD model in all dimensions (p = 0.01), except the dental SLA 3D printer with one of the polymers tested (Bis-GMA) which was not significantly different in two (x and z) dimensions (p = 0.4 and p = 0.12). CONCLUSIONS: The milling technology tested provided greater dimensional accuracy than the selected 3D printing. Printer, printing technology, and material selection affected the accuracy of the printed model.


Asunto(s)
Diseño Asistido por Computadora , Estereolitografía , Polímeros , Impresión Tridimensional
6.
Plant Biotechnol J ; 19(10): 2113-2125, 2021 10.
Artículo en Inglés | MEDLINE | ID: mdl-34076337

RESUMEN

Current approaches for oral health care rely on procedures that are unaffordable to impoverished populations, whereas aerosolized droplets in the dental clinic and poor oral hygiene may contribute to spread of several infectious diseases including COVID-19, requiring new solutions for dental biofilm/plaque treatment at home. Plant cells have been used to produce monoclonal antibodies or antimicrobial peptides for topical applications to decrease colonization of pathogenic microbes on dental surface. Therefore, we investigated an affordable method for dental biofilm disruption by expressing lipase, dextranase or mutanase in plant cells via the chloroplast genome. Antibiotic resistance gene used to engineer foreign genes into the chloroplast genome were subsequently removed using direct repeats flanking the aadA gene and enzymes were successfully expressed in marker-free lettuce transplastomic lines. Equivalent enzyme units of plant-derived lipase performed better than purified commercial enzymes against biofilms, specifically targeting fungal hyphae formation. Combination of lipase with dextranase and mutanase suppressed biofilm development by degrading the biofilm matrix, with concomitant reduction of bacterial and fungal accumulation. In chewing gum tablets formulated with freeze-dried plant cells, expressed protein was stable up to 3 years at ambient temperature and was efficiently released in a time-dependent manner using a mechanical chewing simulator device. Development of edible plant cells expressing enzymes eliminates the need for purification and cold-chain transportation, providing a potential translatable therapeutic approach. Biofilm disruption through plant enzymes and chewing gum-based delivery offers an effective and affordable dental biofilm control at home particularly for populations with minimal oral care access.


Asunto(s)
COVID-19 , Goma de Mascar , Biopelículas , Cloroplastos , Atención a la Salud , Humanos , SARS-CoV-2
7.
J Prosthet Dent ; 126(5): 698-702, 2021 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-33121822

RESUMEN

STATEMENT OF PROBLEM: Optimal composite resin bonds to polymer-infiltrated ceramic network (PICN) computer-aided design and computer-aided manufacturing (CAD-CAM) materials are essential for clinical success. However, comparative bond strength studies on the influence of different etching and cleaning methods on these materials are lacking. PURPOSE: The purpose of this in vitro study was to measure and compare the microshear bond strength (µSBS) of a composite resin cement with that of a PICN material after different surface treatment and cleaning methods. MATERIAL AND METHODS: Seventy specimens of a CAD-CAM PICN were divided into 7 groups (n=10): no treatment (control), hydrofluoric acid etching for 20 seconds (HF20), 60 seconds (HF60), 120 seconds (HF120), HF20 + phosphoric acid for 60 seconds and ultrasonic bath for 5 minutes, HF60 + PH, and HF120 + PH. After surface treatment, a silane coupling agent and composite resin cement were applied. Microshear bond strength was determined, and data were analyzed with 1-way ANOVA and Tukey post hoc multiple comparison tests (α=.05). RESULTS: All HF acid treatments resulted in a significant increase in bond strength to the polymer-infiltrated ceramic network material (P=.02). Bond strength values for HF etching for 20 seconds were significantly lower than those for 60 seconds and 120 seconds (P=.034). No difference was found between 60 seconds and 120 seconds of HF etching time (P=.986). Additional surface treatment with phosphoric acid 60 seconds and ultrasonic bath 5 minutes did not improve the bond strength beyond values obtained by hydrofluoric acid treatment only (P=.834). Most failures were cohesive. CONCLUSIONS: Acid etching and surface treatment have significant effects on composite resin bond strength to a PICN CAD-CAM material. HF etching for 60 seconds or 120 seconds provides the highest bond strengths. Cleaning methods after etching did not have any significant effect on bond strength.


Asunto(s)
Recubrimiento Dental Adhesivo , Cerámica , Diseño Asistido por Computadora , Porcelana Dental , Ácido Fluorhídrico , Ensayo de Materiales , Polímeros , Cementos de Resina , Silanos , Propiedades de Superficie
8.
Clin Oral Implants Res ; 30(2): 178-186, 2019 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-30629769

RESUMEN

OBJECTIVE: This study evaluated the loading capacity of CAD/CAM-fabricated anterior feldspathic ceramic crowns bonded to one-piece zirconia implants with different cements. MATERIAL AND METHODS: Fifty one-piece zirconia implants were embedded in epoxy resin. The abutment aspect of one implant was optically scanned and a standardized upper canine was designed with CAD-software. Fifty feldspathic ceramic crowns were milled, polished, and mounted on the implants either without any cement, with a temporary cement or with three different composite resin cements after surface pretreatment as recommended by the manufacturers (n = 10). After storage in distilled water at 37°C for 24 hr, specimens were loaded until fracture on the palatal surface of the crown at an angle of 45° to the long axis of the implant and loads until fracture were detected and compared. Compressive strength of the investigated cement materials was determined. Statistical analyses were done with One-way ANOVA followed by post hoc Fisher LSD test (α = 0.05). RESULTS: The cements revealed significantly different compressive strength values (temporary cement: 37.1 ± 7.0 MPa; composite resin cements: 185.8 ± 21.3, 277.9 ± 22.1, and 389.0 ± 13.6 MPa, respectively). Load-at-fracture values had an overall mean value of 237.1 ± 58.2 N with no significant difference among the composite resin cements (p > 0.05). Fracture load values with the temporary cement or without cement were significantly lower (p < 0.002). CONCLUSIONS: CAD/CAM-fabricated anterior feldspathic ceramic crowns bonded to one-piece zirconia implants provide sufficient resistance to intraoral forces.


Asunto(s)
Silicatos de Aluminio , Compuestos Corona , Cementos Dentales , Compuestos de Potasio , Prótesis e Implantes , Circonio , Cerámica , Recubrimiento Dental Adhesivo , Soporte de Peso
9.
J Esthet Restor Dent ; 30(6): 551-556, 2018 11.
Artículo en Inglés | MEDLINE | ID: mdl-30378235

RESUMEN

OBJECTIVE: To evaluate the influence of saliva contamination and cleaning procedures on shear bond strength (SBS) of a self-adhesive resin cement (SAC) to zirconia surfaces. MATERIALS AND METHODS: A total of 160 sandblasted zirconia blocks were randomly divided into eight groups as follows: No saliva contamination, no cleansing (NC-NC); contamination with saliva, no cleansing (SC-NC); no saliva contamination, cleansing with a zirconia primer (ZP; Z-Bond, Danville Materials, Inc., S. Ramon, California) (NC-ZP); contamination with saliva, cleansing with ZP (SC-ZP); no saliva contamination, cleansing with hydrofluoric acid (HF; Ultradent Porcelain Etch; Ultradent Products, South Jordan, Utah) followed by cleansing with ZP (NC-HF-ZP); contamination with saliva, cleansing with HF followed by cleansing with ZP (SC-HF-ZP); cleansing with ZP, contamination with saliva, cleansing with ZP (ZP-SC-ZP); application of ZP, contamination with saliva, cleansing with HF followed by cleansing with ZP (ZP-SC-HF-ZP). Cylindrical resin composite blocks were luted to the zirconia surfaces with SAC (Clearfil SA Cement Automix, Kuraray, Inc., Tokyo, Japan). Specimens were subjected to shear forces at a cross-head speed of 0.5 mm/min. Data were analyzed with Analysis of Variance and Tukey tests (α = 0.05). RESULTS: The bond strength values to zirconia were significantly influenced by saliva contamination (P < .05). The SC-NC group showed the lowest bond strength values (5.6 ± 1.4 MPa; P < .05). All cleansing or pretreatment agents improved the bond strengths when compared to noncleansing groups, NC-NC and SC-NC. CONCLUSION: In situations where saliva contamination is deemed unavoidable, application of ZP after try-in of the zirconia restoration could be beneficial for the accurate cementation. CLINICAL SIGNIFICANCE: During try-in sessions of fixed dental prostheses, zirconia ceramic restoration may come into contact with saliva and surfaces should be cleansed to obtain an optimal surface for adhesion. Application of zirconia primer to the sandblasted zirconia surface is recommended whether the surface is contaminated with saliva or not.


Asunto(s)
Recubrimiento Dental Adhesivo , Análisis del Estrés Dental , Japón , Ensayo de Materiales , Cementos de Resina , Saliva , Propiedades de Superficie , Circonio
10.
J Prosthet Dent ; 119(6): 987-993, 2018 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-29042115

RESUMEN

STATEMENT OF PROBLEM: A recommended minimum thickness for monolithic zirconia restorations has not been reported. Assessing a proper thickness that has the necessary load-bearing capacity but also conserves dental hard tissues is essential. PURPOSE: The purpose of this in vitro study was to evaluate the effect of thickness and surface modifications on monolithic zirconia after simulated masticatory stresses. MATERIAL AND METHODS: Monolithic zirconia disks (10 mm in diameter) were fabricated with 1.3 mm and 0.8 mm thicknesses. For each thickness, 21 disks were fabricated. The specimens of each group were further divided into 3 subgroups (n=7) according to the surface treatments applied: untreated (control), airborne-particle abrasion with 50-µm Al2O3 particles at a pressure of 400 kPa at 10 mm, and grinding with a diamond rotary instrument followed by polishing. The biaxial flexure strength was determined by using a piston-on-3-balls technique in a universal testing machine. Flexural loading was applied with a 1.4-mm diameter steel cylinder, centered on the disk, at a crosshead speed of 0.5 mm/min until fracture occurred. X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses were performed. The data were statistically analyzed with 2-way ANOVA, Tamhane T2, 1-way ANOVA, and Student t tests (α=.05). RESULTS: The 1.3-mm specimens had significantly higher flexural strength than the 0.8-mm specimens (P<.05). Airborne-particle abrasion significantly increased the flexural strength (P<.05). Grinding and polishing did not affect the flexural strength of the specimens (P>.05). CONCLUSIONS: The mean flexural strength of 0.8-mm and 1.3-mm thick monolithic zirconia was greater than reported masticatory forces. Airborne-particle abrasion increased the flexural strength of monolithic zirconia. Grinding did not affect flexural strength if subsequently polished.


Asunto(s)
Circonio , Resistencia Flexional , Masticación , Difracción de Rayos X
11.
Clin Oral Investig ; 17(3): 793-8, 2013 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-22782256

RESUMEN

OBJECTIVES: This study evaluated and compared sensitivity of teeth after cementation of full-coverage crowns with a new self-adhesive resin cement (SARC). A resin-modified glass ionomer cement (RMGIC) served as control. MATERIALS AND METHODS: Eighty-eight full-coverage crowns were cemented to vital teeth with either the self-adhesive cement iCem (Heraeus Kulzer; n = 44) or the RMGIC GC Fuji PLUS (GC, n = 44). Before preparations, patients were questioned for sensitivity (patient sensitivity, PS). In addition, air was blown for 2 s onto the buccal cementoenamel junction (air sensitivity, AS), and ice spray was applied in the cementoenamel junction area (ice sensitivity, IS). Patient responses were recorded with a visual analog scale. After cementation of the crowns, patients were recalled for follow-up (f/u) visits at 1 day, 1 week, and 3 weeks. PS, AS, and IS were recorded during each visit. Data were analyzed with Mann-Whitney U tests. RESULTS: The two groups revealed comparable sensitivity scores at baseline. SARC showed significantly lower PS sensitivity scores at 1 day (p = 0.02) and significantly lower AS scores at 1-week follow-up (p = 0.01). IS generally produced the highest sensitivity scores with SARC revealing significantly lower scores at all follow-up visits. CONCLUSION: Cementation of crowns with the SARC tested in this study resulted in overall lower postoperative sensitivity than with the RMGIC. CLINICAL RELEVANCE: Among other clinical advantages, some self-adhesive resin cements seem to lower postoperative sensitivity of crowned teeth.


Asunto(s)
Sensibilidad de la Dentina/prevención & control , Cementos de Resina/uso terapéutico , Adulto , Anciano , Cementación/efectos adversos , Coronas , Sensibilidad de la Dentina/etiología , Femenino , Cementos de Ionómero Vítreo/uso terapéutico , Humanos , Masculino , Persona de Mediana Edad , Complicaciones Posoperatorias/prevención & control , Estudios Prospectivos , Estadísticas no Paramétricas
12.
Materials (Basel) ; 16(7)2023 Mar 24.
Artículo en Inglés | MEDLINE | ID: mdl-37048873

RESUMEN

Head and neck cancers are a significant global health burden, with radiation therapy being a frequently utilized treatment. The aim of this systematic review was to provide a critical appraisal of laboratory studies that assessed the effect of irradiation on the adhesive performance of resin-based biomaterials. The analysis included 23 laboratory studies obtained from five databases, with most studies using human enamel, dentin, or both, and bonding procedures involving the fabrication of direct restorations, standardized specimens, bonding of orthodontic brackets, and luting of endodontic fiber posts. The protocols used for irradiation varied, with most studies exposing specimens made from extracted teeth to irradiation using cabinet irradiators to simulate treatment of head and neck cancer. The findings indicate that irradiation reduces the bond strength of dental adhesives and resin-based composites on flat, ground enamel and dentin specimens, with different adhesives and timing of irradiation having a significant impact on adhesive performance. Irradiation also increased microleakage in most studies. The effect of irradiation on marginal adaptation of direct resin-based composite restorations was inconclusive. This systematic review indicates that irradiation has detrimental effects on the adhesive performance of resin-based biomaterials and highlights the need for further clinical and laboratory studies evaluating the performance of adhesive materials and approaches to improve it.

13.
Int J Prosthodont ; 35(6): 777­783, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-33616569

RESUMEN

PURPOSE: To determine and compare the mechanical properties of 3D-printed yttriastabilized zirconia to milled isostatic pressed yttria-stabilized zirconia, with the following hypotheses: (1) The flexural strength of 3D-printed yttria-stabilized zirconia is comparable to milled yttria-stabilized isostatic pressed zirconia; and (2) thermocycling and chewing simulation do not affect the flexural strength of 3D-printed yttria-stabilized zirconia. MATERIALS AND METHODS: A total of 30 bars of an experimental 3D-printed 3 mol% yttriastabilized zirconia (LithaCon 3Y 230, Lithoz) and 10 bars of milled isostatic pressed zirconia (Prettau Zirconia, Zirkonzahn) were utilized. The printed zirconia bars were divided into three groups (n = 10 bars per group): (1) untreated (control); (2) thermocycled; and (3) tested after chewing simulation. A flexural strength test was performed on all samples using a three-point bend test in an Instron Universal testing machine. One-way analysis of variance on ranks was used to compare milled to printed zirconia. The effects of thermocycling and load cycling on 3D-printed zirconia were also determined. RESULTS: The flexural strength values for milled and printed zirconia were 936.3 ± 255.0 MPa and 855.4 ± 112.6 MPa, respectively. There was no statistically significant difference in flexural strength between the milled and printed zirconia (P = .178). No statistically significant differences were observed between the control 3D-printed zirconia group and the thermocycled (888.4 ± 59.3 MPa) or load-cycled printed zirconia (789.6 ± 133.8 MPa; P = .119). CONCLUSION: Printed 3 mol% yttria-stabilized zirconia has comparable flexural strength to milled yttria-stabilized isostatic pressed zirconia. The thermocycling and chewing simulation used in this study did not significantly alter the flexural strength of the printed 3 mol% yttria-stabilized zirconia. These results indicate a promising role for 3D printing in the fabrication of zirconia restorations. Additional studies are needed to explore the full potential of this technology.

14.
Int J Dent ; 2022: 3215048, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35342425

RESUMEN

Objective: To determine the effects of preirradiation fluoride treatments on the Knoop hardness of dentin. Materials and Methods: Human posterior teeth mounted into acrylic resin molds were polished with silicon carbide (SiC) abrasives and 3-micron diamond paste. The Knoop hardness of dentin was measured with a Leco hardness instrument. The teeth were divided into groups of ten teeth per group as follows: no treatment (control), treatment with silver diamine fluoride (SDF), MI varnish (MI), and cavity shield (CS). The teeth were exposed to 2 Gy of daily radiation for six weeks using an X-Rad 320ix biological irradiator. Hardness was measured weekly, before, during, and after irradiation. The teeth were stored in artificial saliva at 37oC between radiation treatments. Results: In preirradiation dentin, a Knoop hardness value of 58.8 (14.1) KHN was obtained. Treatment with SDF significantly increased KHN before irradiation. Immediately after radiation treatment, hardness was significantly reduced in all experimental groups. Postirradiation fluoride treatments increased the hardness of dentin to varying degrees. Conclusions: Preirradiation fluoride treatment does not provide protection from decreases in the hardness of dentin. Treatment of teeth with fluoride formulations after radiation progressively restores the hardness of dentin to different degrees.

15.
Biomaterials ; 288: 121671, 2022 09.
Artículo en Inglés | MEDLINE | ID: mdl-35953331

RESUMEN

Because oral transmission of SARS-CoV-2 is 3-5 orders of magnitude higher than nasal transmission, we investigated debulking of oral viruses using viral trap proteins (CTB-ACE2, FRIL) expressed in plant cells, delivered through the chewing gum. In omicron nasopharyngeal (NP) samples, the microbubble count (based on N-antigen) was significantly reduced by 20 µg of FRIL (p < 0.0001) and 0.925 µg of CTB-ACE2 (p = 0.0001). Among 20 delta or omicron NP samples, 17 had virus load reduced below the detection level of spike protein in the RAPID assay, after incubation with the CTB-ACE2 gum powder. A dose-dependent 50% plaque reduction with 50-100 ng FRIL or 600-800 µg FRIL gum against Influenza strains H1N1, H3N2, and Coronavirus HCoV-OC43 was observed with both purified FRIL, lablab bean powder or gum. In electron micrographs, large/densely packed clumps of overlapping influenza particles and FRIL protein were observed. Chewing simulator studies revealed that CTB-ACE2 release was time/dose-dependent and release was linear up to 20 min chewing. Phase I/II placebo-controlled, double-blinded clinical trial (IND 154897) is in progress to evaluate viral load in saliva before or after chewing CTB-ACE2/placebo gum. Collectively, this study advances the concept of chewing gum to deliver proteins to debulk oral viruses and decrease infection/transmission.


Asunto(s)
COVID-19 , Subtipo H1N1 del Virus de la Influenza A , Gripe Humana , Enzima Convertidora de Angiotensina 2 , Goma de Mascar , Procedimientos Quirúrgicos de Citorreducción , Humanos , Subtipo H3N2 del Virus de la Influenza A , Proteínas de Plantas , Polvos , SARS-CoV-2 , Proteínas Virales
16.
J Prosthodont ; 20(4): 286-93, 2011 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-21539646

RESUMEN

PURPOSE: The retentive forces and the strain energies absorbed during dislodging of implant overdenture stud attachments are useful parameters to consider in the selection of attachments. The purpose of this study was to compare the retentive forces and strain energies of the Nobel Biocare standard ball, Nobel Biocare newer generation ball (Yorba Linda, CA), Zest Anchor, Zest Anchor Advanced Generation (Escondido, CA), Sterngold-Implamed ERA white, and Sterngold-Implamed orange attachments (Attleboro, MA) on an implant-retained in vitro overdenture model. MATERIALS AND METHODS: The attachments were tested using two permanently placed Brånemark system implants on a test model attached to an Instron machine. Each attachment had one part embedded in a denture-like housing, and the other part screwed into the implants. Dislodging tensile forces were applied to the housings in two directions simulating function: vertical and oblique. Eight tests were done in two directions with six specimens of each attachment. Retentive forces generated and strain energies absorbed during displacement were determined. A 1-way ANOVA followed by the Tukey studentized range test was used to determine groups that were significantly different at the p < 0.05 level. RESULTS: The Zest Anchor Advanced Generation attachment had significantly the highest retentive vertical and oblique forces [37.2 (5.5) N and 25.9 (3.2) N, respectively]. The Zest Anchor had the lowest vertical force [10.8 (4.2) N], and Nobel Biocare Standard had the lowest oblique retentive force [10.6 (3.0) N]. The Nobel Biocare Standard Ball attachment had the highest strain energies [29.7 × 10(-3) (11.9 × 10(-3)) J, 30.3 × 10(-3) (14.3 × 10(-3)) J, respectively, in the vertical and oblique directions]. The Sterngold-Implamed ERA White and Zest Anchor had the lowest strain energies [5.3 × 10(-3) (3.2 × 10(-3)) J and 4.5 × 10(-3) (1.1 × 10(-3)) J, respectively, in the vertical and oblique directions]. CONCLUSION: The retentive forces and strain energies of implant overdenture stud attachments are different and should be considered during prosthesis selection.


Asunto(s)
Prótesis Dental de Soporte Implantado , Análisis del Estrés Dental , Ajuste de Precisión de Prótesis , Retención de Dentadura/instrumentación , Prótesis de Recubrimiento , Pilares Dentales , Implantes Dentales , Diseño de Prótesis Dental , Mandíbula , Ensayo de Materiales , Modelos Dentales , Estrés Mecánico
17.
N Y State Dent J ; 77(1): 23-6, 2011 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21417162

RESUMEN

This study investigated the effect of 2% chlorhexidine gluconate (CHX) disinfectant on bond strength (BS) of high-density glass ionomer cement (HDGIC) to dentin following atraumatic restorative treatment (ART) and conventional preparations. Specimens were divided into four groups: Group 1--ART (control); Group 2--ART with CHX disinfection; Group 3--Conventional (control); Group 4--Conventional with CHX disinfection. HDGIC was packed in cylindrical molds placed over flat dentin surfaces; BS was measured after seven days. ART-prepared dentin surfaces disinfected with CHX provided bonding to HDGIC that was comparable to untreated dentin and to conventionally prepared dentin.


Asunto(s)
Antiinfecciosos Locales/química , Clorhexidina/química , Tratamiento Restaurativo Atraumático Dental/métodos , Recubrimiento Dental Adhesivo , Dentina/ultraestructura , Cementos de Ionómero Vítreo/química , Tratamiento Restaurativo Atraumático Dental/instrumentación , Preparación de la Cavidad Dental/clasificación , Preparación de la Cavidad Dental/instrumentación , Restauración Dental Permanente/instrumentación , Restauración Dental Permanente/métodos , Análisis del Estrés Dental/instrumentación , Humanos , Ensayo de Materiales , Resistencia al Corte , Estrés Mecánico , Propiedades de Superficie , Factores de Tiempo
18.
Acta Stomatol Croat ; 55(1): 76-89, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33867540

RESUMEN

OBJECTIVE: Ion-incorporated zeolite is a widely used antimicrobial material studied for various dental applications. At present, there is no other systematic review that evaluates the effectiveness of zeolite in all dental materials. The purpose of this study was to review all available literature that analyzed the antimicrobial effects and/or mechanical properties of zeolite as a restorative material in dentistry. MATERIAL AND METHODS: Following PRISMA guidelines, an exhaustive search of PubMed, Ovid Medline, Scopus, Embase, and the Dentistry & Oral Sciences Source was conducted. No language or time restrictions were used and the study was conducted from June 1, 2020 to August 17, 2020. Only full text articles were selected that pertained to the usage of zeolite in dental materials including composite resin, bonding agents, cements, restorative root material, cavity base material, prosthesis, implants, and endodontics. RESULTS: At the beginning of the study, 1534 studies were identified, of which 687 duplicate records were excluded. After screening for the title, abstract, and full texts, 35 articles remained and were included in the qualitative synthesis. An Inter-Rater Reliability (IRR) test, which included a percent user agreement and reliability percent, was conducted for each of the 35 articles chosen. CONCLUSION: Although ion-incorporated zeolite may enhance the antimicrobial properties of dental materials, the mechanical properties of some materials, such as MTA and acrylic resin, may be compromised. Therefore, since the decrease in mechanical properties depends on zeolite concentration in the restorative material, it is generally recommended to add 0.2-2% zeolite by weight.

19.
Clin Oral Investig ; 14(2): 187-92, 2010 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-19415350

RESUMEN

This study compared shear bond strengths of six self-adhesive resin cements to zirconium oxide ceramic with and without air-particle abrasion. One hundred twenty zirconia samples were air-abraded (group SB; n = 60) or left untreated (group NO). Composite cylinders were bonded to the zirconia samples with either BisCem (BC), Maxcem (MC), G-Cem (GC), RelyX Unicem Clicker (RUC), RelyX Unicem Applicator (RUA), or Clearfil SA Cement (CSA). Shear bond strength was tested after thermocycling, and data were analyzed with analysis of variance and Holm-Sidak pairwise comparisons. Without abrasion, RUA (8.0 MPa), GC (7.9 MPa), and CSA (7.6 MPa) revealed significantly higher bond strengths than the other cements. Air-particle abrasion increased bond strengths for all test cements (p < 0.001). GC (22.4 MPa) and CSA (18.4 MPa) revealed the highest bond strengths in group SB. Bond strengths of self-adhesive resin cements to zirconia were increased by air-particle abrasion. Cements containing adhesive monomers (MDP/4-META) were superior to other compositions.


Asunto(s)
Recubrimiento Dental Adhesivo , Grabado Dental/métodos , Porcelana Dental/química , Cementos de Resina/química , Circonio/química , Aire , Resinas Compuestas/química , Análisis del Estrés Dental/instrumentación , Humanos , Ensayo de Materiales , Metacrilatos/química , Compuestos Organofosforados/química , Resistencia al Corte , Estrés Mecánico , Propiedades de Superficie , Temperatura , Factores de Tiempo
20.
Am J Orthod Dentofacial Orthop ; 138(4): 463-467, 2010 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-20889052

RESUMEN

INTRODUCTION: The aim of this study was to evaluate the frictional resistance between active and passive self-ligating brackets and 0.019 × 0.025-in stainless steel archwire during sliding mechanics by using an orthodontic sliding simulation device. METHODS: Maxillary right first premolar active self-ligating brackets In-Ovation R, In-Ovation C (both, GAC International, Bohemia, NY), and SPEED (Strite Industries, Cambridge, Ontario, Canada), and passive self-ligating brackets SmartClip (3M Unitek, Monrovia, Calif), Synergy R (Rocky Mountain Orthodontics, Denver, Colo), and Damon 3mx (Ormco, Orange, Calif) with 0.022-in slots were used. Frictional force was measured by using an orthodontic sliding simulation device attached to a universal testing machine. Each bracket-archwire combination was tested 30 times at 0° angulation relative to the sliding direction. Statistical comparisons were performed with 1-way analysis of variance (ANOVA) followed by Dunn multiple comparisons. The level of statistical significance was set at P <0.05. RESULTS: The Damon 3mx brackets had significantly the lowest mean static frictional force (8.6 g). The highest mean static frictional force was shown by the SPEED brackets (83.1 g). The other brackets were ranked as follows, from highest to lowest, In-Ovation R, In-Ovation C, SmartClip, and Synergy R. The mean static frictional forces were all statistically different. The ranking of the kinetic frictional forces of bracket-archwire combinations was the same as that for static frictional forces. All bracket-archwire combinations showed significantly different kinetic frictional forces except SmartClip and In-Ovation C, which were not significantly different from each other. CONCLUSIONS: Passive self-ligating brackets have lower static and kinetic frictional resistance than do active self-ligating brackets with 0.019 × 0.025-in stainless steel wire.


Asunto(s)
Diseño de Aparato Ortodóncico , Soportes Ortodóncicos , Alambres para Ortodoncia , Análisis de Varianza , Análisis del Estrés Dental , Fricción , Ensayo de Materiales , Acero Inoxidable
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