RESUMEN
An electron microscope study has been carried out on rat psoas muscle, during the early postnatal stages of development. Among the several subcellular components, the sarcotubular system undergoes the most striking modifications during this period. In muscle fibers of the newborn rat, junctional contacts between the T system and the SR are sparse and are, mostly, longitudinally or obliquely oriented. The T tubules do not penetrate deeply into the muscle cell, as indicated by the predominantly peripheral location of the triads and the persistence, at these stages of development, of a highly branched subsarcolemmal system of tubules. Diadic associations of junctional SR elements with the plasma membrane are also occasionally observed. The early SR elaborations incompletely delineate the myofibrils, at both the A- and I-band level. Longitudinal sections show irregularly oriented SR tubules, running continuously over successive sarcomeres. Flattened junctional cisterns filled with granular material are sparse and laterally interconnected, at circumscribed sites, with the SR tubules. Between 1 and 2 wk postpartum, transversal triadic contacts are extensively established, at the A-I band level, and the SR network differentiates into two portions in register with the A and I band, respectively. At 10-15 days after birth, the SR provides a transversely continuous double sheet around the myofibrils at the I-band level, whereas it forms a single discontinuous layer at the A-band level. The relationship that these morphological modifications of the sarcotubular system may bear to previously described biochemical and physiological changes of rat muscle fibers after birth is discussed.
Asunto(s)
Desarrollo de Músculos , Músculos/citología , Miofibrillas , Animales , Animales Recién Nacidos , Diferenciación Celular , Aparato de Golgi , Mitocondrias , Ratas , RibosomasRESUMEN
Transverse tubule (TT) membranes isolated from chicken skeletal muscle possess a very active magnesium-stimulated ATPase (Mg-ATPase) activity. The Mg-ATPase has been tentatively identified as a 102-kD concanavalin A (Con A)-binding glycoprotein comprising 80% of the integral membrane protein (Okamoto, V.R., 1985, Arch. Biochem. Biophys., 237:43-54). To firmly identify the Mg-ATPase as the 102-kD TT component and to characterize the structural relationship between this protein and the closely related sarcoplasmic reticulum (SR) Ca-ATPase, polyclonal antibodies were raised against the purified SR Ca-ATPase and the TT 102-kD glycoprotein, and the immunological relationship between the two ATPases was studied by means of Western immunoblots and enzyme-linked immunosorbent assays (ELISA). Anti-chicken and anti-rabbit SR Ca-ATPase antibodies were not able to distinguish between the TT 102-kD glycoprotein and the SR Ca-ATPase. The SR Ca-ATPase and the putative 102-kD TT Mg-ATPase also possess common structural elements, as indicated by amino acid compositional and peptide mapping analyses. The two 102-kD proteins exhibit similar amino acid compositions, especially with regard to the population of charged amino acid residues. Furthermore, one-dimensional peptide maps of the two proteins, and immunoblots thereof, show striking similarities indicating that the two proteins share many common epitopes and peptide domains. Polyclonal antibodies raised against the purified TT 102-kD glycoprotein were localized by indirect immunofluorescence exclusively in the TT-rich I bands of the muscle cell. The antibodies substantially inhibit the Mg-ATPase activity of isolated TT vesicles, and Con A pretreatment could prevent antibody inhibition of TT Mg-ATPase activity. Further, the binding of antibodies to intact TT vesicles could be reduced by prior treatment with Con A. We conclude that the TT 102-kD glycoprotein is the TT Mg-ATPase and that a high degree of structural homology exists between this protein and the SR Ca-ATPase.
Asunto(s)
ATPasa de Ca(2+) y Mg(2+)/metabolismo , ATPasas Transportadoras de Calcio/metabolismo , Retículo Sarcoplasmático/enzimología , Animales , ATPasa de Ca(2+) y Mg(2+)/aislamiento & purificación , ATPasas Transportadoras de Calcio/aislamiento & purificación , Pollos , Concanavalina A , Técnica del Anticuerpo Fluorescente , Microsomas/enzimología , Músculos/citología , Músculos/enzimología , Retículo Sarcoplasmático/ultraestructuraRESUMEN
The presence and distribution of calsequestrin (CS), Ca2+ pump, and inositol 1,4,5-trisphosphate (IP3) receptor were investigated biochemically and immunologically in microsomal (P3) fractions isolated from chicken cerebrum and cerebellum. Two different batches of polyclonal antibodies specific for chicken skeletal muscle CS identified a Ca2+ binding, CS-like protein that was extremely enriched in cerebellum P3 fractions and absent from all cerebrum fractions. The cerebellum CS-like protein was deemed authentic CS because the N-terminal amino acid domain and peptide mapping were identical to those of skeletal muscle CS in the same species. CS was detected in striated muscles and cerebellum only. Cerebellum P3 fractions were also found to be considerably enriched in Ca2+ pump and IP3 receptor compared with the homologous cerebrum fractions, as judged by measurements of Ca2+ uptake, Ca2(+)-ATPase activity, IP3-induced Ca2+ release, and [3H]IP3 binding, respectively. Cerebellum microsomal fractions therefore appear to contain membrane fragments endowed with Ca2+ pump, IP3 receptor, and CS, i.e., three key components of a Ca2+ storage organelle.
Asunto(s)
Calcio/metabolismo , Calsecuestrina/metabolismo , Cerebelo/metabolismo , Inositol 1,4,5-Trifosfato/farmacología , Animales , ATPasas Transportadoras de Calcio/metabolismo , Calsecuestrina/química , Pollos , Técnicas Inmunológicas , Microsomas/metabolismo , Mapeo Peptídico , Distribución TisularRESUMEN
Brittle deformation can saturate the Earth's crust with faults and fractures in an apparently chaotic fashion. The details of brittle deformational histories and implications on, for example, seismotectonics and landscape, can thus be difficult to untangle. Fortunately, brittle faults archive subtle details of the stress and physical/chemical conditions at the time of initial strain localization and eventual subsequent slip(s). Hence, reading those archives offers the possibility to deconvolute protracted brittle deformation. Here we report K-Ar isotopic dating of synkinematic/authigenic illite coupled with structural analysis to illustrate an innovative approach to the high-resolution deconvolution of brittle faulting and fluid-driven alteration of a reactivated fault in western Norway. Permian extension preceded coaxial reactivation in the Jurassic and Early Cretaceous fluid-related alteration with pervasive clay authigenesis. This approach represents important progress towards time-constrained structural models, where illite characterization and K-Ar analysis are a fundamental tool to date faulting and alteration in crystalline rocks.
RESUMEN
Systematic immunological and biochemical studies indicate that the level of expression of sarcoplasmic reticulum (SR) Ca(2+)-ATPase regulatory protein phospholamban (PLB) in mammalian slow-twitch fibers varies from zero, in the rat, to significant levels in the rabbit, and even higher in humans. The lack of PLB expression in the rat, at the mRNA level, is shown to be exclusive to slow-twitch skeletal muscle, and not to be shared by the heart, thus suggesting a tissue-specific, in addition to a species-specific regulation of PLB. A comparison of sucrose density-purified SR of rat and rabbit slow-twitch muscle, with regard to protein compositional and phosphorylation properties, demonstrates that the biodiversity is two-fold, i.e. (a) in PLB membrane density; and (b) in the ability of membrane-bound Ca(2+)-calmodulin (CaM)-dependent protein kinase II to phosphorylate both PLB and SERCA2a (slow-twitch isoform of Ca(2+)-ATPase). The basal phosphorylation state of PLB at Thr-17 in isolated SR vesicles from rabbit slow-twitch muscle, colocalization of CaM K II with PLB and SERCA2a at the same membrane domain, and the divergent subcellular distribution of PKA, taken together, seem to argue for a differential heterogeneity in the regulation of Ca(2+) transport between such muscle and heart muscle.
Asunto(s)
Proteínas de Unión al Calcio/metabolismo , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Músculo Esquelético/metabolismo , Miocardio/metabolismo , Animales , Peso Corporal , Proteína Quinasa Tipo 1 Dependiente de Calcio Calmodulina , Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina , ATPasas Transportadoras de Calcio/metabolismo , Técnica del Anticuerpo Fluorescente , Cobayas , Humanos , Masculino , Ratones , Fibras Musculares Esqueléticas/metabolismo , Fenotipo , Fosforilación , Conejos , Ratas , Ratas Wistar , Retículo Sarcoplasmático/metabolismo , Especificidad de la Especie , Especificidad por SustratoRESUMEN
We have shown that the isolated sarcoplasmic reticulum from rabbit slow muscle contains cytochrome b5 which can be reduced via a flavoprotein, with FAD as the prosthetic group. In the presence of NADH and oxygen, these sarcoplasmic reticulum membranes can convert stearyl-CoA to oleyl-CoA, similarly to liver endoplasmic reticulum membranes. However, the stearyl-CoA desaturase system is virtually lacking in fast muscle sarcoplasmic reticulum. The data suggest that these differences between fast and slow twitch muscle may be related to the characteristic fatty acid composition of phospholipids and the function of the sarcoplasmic reticulum.
Asunto(s)
Ácido Graso Desaturasas/metabolismo , Retículo Sarcoplasmático/enzimología , Estearoil-CoA Desaturasa/metabolismo , Animales , Reductasas del Citocromo/metabolismo , Citocromos/metabolismo , Técnicas In Vitro , Masculino , Mitocondrias Musculares/enzimología , ConejosRESUMEN
The tibialis anterior (TA) muscles of 6-month-old and 24-month-old male Wistar rats, after being characterized, at the fast motor unit level, for twitch properties, were dissected and processed by a procedure [Margreth A., Damiani E., Tobaldin G. Biochem Biophys Res Commun 1993; 197: 1303-1311] aimed at obtaining a representative total membrane fraction comprising 70-80% of the total muscle content of sarcoplasmic reticulum (SR) and transverse tubule (TT) membranes (about 20 mg protein/g). Skeletal muscle membranes were analyzed for protein composition, and the content and functional properties of specific components of the free and junctional subcompartments of the SR and of junctional TT. Our results, while confirming a twitch prolongation in TA of old rats, do not demonstrate any associated age-related change concerning: (a) the overall number and functional properties of Ca2+ pumps, as characterized by kinetic parameters, Ca(2+)-dependency, and the protein isoform specificity of SR Ca(2+)-ATPase; (b) the number of functional junctional SR Ca(2+)-release channels, on the basis of Bmax values for high-affinity binding of [3H]-ryanodine to skeletal muscle membranes at optimal Ca2+; (c) the overall muscle dihydropyridine receptor/ryanodine receptor (RyR) ratio. We conclude from these findings, and the additional negative evidence for changes in membrane density of specific components of junctional SR, including 60 kDa Ca(2+)-calmodulin protein kinase, that this membrane domain, like the Ca(2+)-pump domain of the SR, are in no way basically altered at early stages of the aging process, as investigated here. Because of that, we allege particular significance to the occurrence of age-related, specific abnormalities in regulation of RyR in rat TA. The main supportive evidence is as follows: (a) an increased sensitivity to Ca2+ of the RyR of old muscle, and, more importantly; (b) an increased sensitivity to caffeine of [3H]ryanodine binding to the RyR at optimal Ca2+ and also optimal for the activity of the Ca(2+)-release channel. The results reported here also demonstrate that there are two classes of caffeine sites in rat TA muscle, as defined by differences in EC50 values at resting (pCa 7) and at high Ca2+ (pCa 4-5), that sites involved in stimulation of [3H]-ryanodine binding to the RyR are distinguished by a higher affinity (caffeine below mM), and that only these sites undergo age-related changes. Thus, although the underlying age-related abnormality of the RyR remains to be elucidated, it appears to satisfy the requirement for being regarded as a specific change, which in itself might argue for its being fundamentally related to the twitch prolongation of the muscle.
Asunto(s)
Envejecimiento/fisiología , Canales de Calcio/fisiología , Fibras Musculares de Contracción Rápida/química , Proteínas Musculares/fisiología , Animales , Antibióticos Antineoplásicos/farmacología , Sitios de Unión/fisiología , Cafeína/farmacología , Calcio/fisiología , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/análisis , Canales de Calcio/metabolismo , Canales de Calcio Tipo L , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , ATPasas Transportadoras de Calcio/metabolismo , Calmodulina/farmacología , Proteínas de Unión a Calmodulina/análisis , Proteínas de Unión a Calmodulina/fisiología , Relación Dosis-Respuesta a Droga , Doxorrubicina/farmacología , Isradipino/farmacología , Cinética , Masculino , Proteínas de la Membrana/análisis , Fibras Musculares de Contracción Rápida/enzimología , Proteínas Musculares/análisis , Músculo Esquelético/química , Músculo Esquelético/citología , Músculo Esquelético/enzimología , Inhibidores de Fosfodiesterasa/farmacología , Ratas , Ratas Wistar , Canal Liberador de Calcio Receptor de Rianodina , Sensibilidad y Especificidad , TritioRESUMEN
We have devised a novel procedure, employing Chaps rather than Triton [Costello B., Chadwick C., Saito A., Chu A., Maurer A., Fleischer S. J Cell Biol 1986; 103: 741-753], for obtaining vesiculated derivatives of the junctional face membrane (JFM) domain of isolated terminal cisternae (TC) from fast skeletal muscle of the rabbit. Enriched JFM is minimally contaminated with junctional transverse tubules. The characteristic ultrastructural features and the most essential features of TC function relating to this membrane domain-i.e. both the Ca(2+)-release system and the Ca2+ and calmodulin (CaM)-dependent protein kinase (CaM I PK) system-appear to be retained in enriched JFM. We show that our isolation procedure, yielding up to a 2.5-fold enrichment in ryanodine receptor (RyR) protein and in the maximum number of high affinity [3H]-ryanodine binding sites, does not alter the assembly for integral proteins associated with the receptor in its native membrane environment, i.e. FKBP-12, triadin and the structurally related protein junction [Jones L.R., Zhang L., Sanborn K., Jorgensen A., Kelley J. J Biol Chem 1995; 270: 30787-30796] having, in common, the property to bind calsequestrin (CS) in overlays in the presence of EGTA. The substrate specificity of endogenous CaM I PK is also the same as that of parent TC vesicles. Phosphorylation of mainly triadin and of a high M(r) polypeptide, and not of the RyR, is the most remarkable common property. Retention of peripheral proteins, like CS and histidine-rich Ca(2+)-binding protein, although not that endogenous CaM, and of a unique set of CaM-binding proteins, unlike that of junctional SR-specific integral proteins, is shown to be influenced by the concentration of Ca2+ during incubation of TC vesicles with Chaps. Characterization of RyR functional behaviour with [3H]-ryanodine has indicated extensive similarities between the enriched JFM and parent TC vessicles, as far as the characteristic bell shaped Ca(2+)-dependence of [3H]-ryanodine binding and the dose-dependent sensitization to Ca2+ by caffeine, reflecting the inherent properties of SR Ca(2+)-release channel, as well as concerning the stimulation of [3H]-ryanodine binding by increasing concentrations of KCl. Stabilizing the RyR in a maximally active state by optimizing concentrations of KCl (1 M), at also optimal concentrations of Ca2+ (pCa 4), rendered the receptor less sensitive to inhibition by 1 microM CaM, to a greater extent in the case of enriched JFM. That was not accounted for by any significant difference in the IC50 concentrations of CaM varying between 40 nM to approximately 80 nM, at low-intermediate and at high KCl concentrations, respectively. Additional results with enriched JFM using doxorubicin, a pharmacological Ca2+ channel allosteric modifier, strengthen the hypothesis that the conformational state at which RyR is stabilized, according to the experimental assay conditions for [3H]-ryanodine binding, directly influences CaM-sensitivity.
Asunto(s)
Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Canal Liberador de Calcio Receptor de Rianodina/metabolismo , Retículo Sarcoplasmático/metabolismo , Animales , Bloqueadores de los Canales de Calcio/farmacología , Proteína Quinasa Tipo 1 Dependiente de Calcio Calmodulina , Calmodulina/metabolismo , Ácidos Cólicos , Digoxigenina , Electroforesis en Gel de Poliacrilamida , Sueros Inmunes , Immunoblotting , Inmunoglobulina G , Membranas Intracelulares/metabolismo , Isradipino/metabolismo , Fibras Musculares de Contracción Rápida/metabolismo , Fosforilación , Conejos , Rianodina/metabolismo , Canal Liberador de Calcio Receptor de Rianodina/efectos de los fármacos , Retículo Sarcoplasmático/efectos de los fármacos , TripsinaRESUMEN
The cardiac and skeletal muscle isoforms of calsequestrin (CS), the low affinity, high capacity Ca2+ binding protein localized in the lumen of sarcoplasmic reticulum, are the products of two different genes (Fliegel, L., Leberer, E., Green, N.M. and MacLennan, D.H. (1982) FEBS Lett. 242, 297-300), and can be both purified from slow-twitch skeletal muscle of the rabbit (Damiani, E., Volpe, P. and Margreth, A. (1990) J. Muscle Res. Cell Motil. 11, 522-530). Here we show that both CS isoforms coexist in slow-twitch muscle fibers as indicated by indirect immunofluorescent staining of cryosections with affinity-purified antibodies specific for each CS isoform.
Asunto(s)
Calsecuestrina/metabolismo , Músculos/metabolismo , Animales , Western Blotting , Electroforesis en Gel de Poliacrilamida , Técnica del Anticuerpo Fluorescente , Isomerismo , Ratones , ConejosRESUMEN
Modulation of sarcoplasmic reticulum (SR) Ca(2+) transport by endogenous calmodulin-dependent protein kinase II (CaM K II) involves covalent changes of regulatory protein phospholamban (PLB), as a common, but not the only mechanism, in limb slow-twitch muscles of certain mammalian species, such as the rabbit. Here, using immunofluorescent techniques in situ, and biochemical and immunological methods on the isolated SR, we have demonstrated that rabbit masseter, a muscle with a distinct embryological origin, lacks PLB. Accommodating embryological heterogeneity in the paradigm of neural-dependent expression of specific isogenes in skeletal muscle fibers, our results provide novel evidence for the differential expression in the SR of 72 kDa beta components of CaM K II, together with the expression of a slow-twitch sarcoendoplasmic reticulum Ca(2+)-ATPase isoform, both in limb muscle and in the masseter.
Asunto(s)
Proteínas de Unión al Calcio/metabolismo , Proteínas Quinasas Dependientes de Calcio-Calmodulina/biosíntesis , ATPasas Transportadoras de Calcio/biosíntesis , Músculo Masetero/enzimología , Fibras Musculares de Contracción Lenta/enzimología , Retículo Sarcoplasmático/enzimología , Animales , Western Blotting , Proteínas de Unión al Calcio/análisis , Proteínas de Unión al Calcio/biosíntesis , Proteínas de Unión al Calcio/inmunología , Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , ATPasas Transportadoras de Calcio/análisis , ATPasas Transportadoras de Calcio/inmunología , Centrifugación por Gradiente de Densidad , Técnica del Anticuerpo Fluorescente Directa , Immunoblotting , Isoenzimas/análisis , Isoenzimas/biosíntesis , Isoenzimas/inmunología , Masculino , Músculo Masetero/metabolismo , Fibras Musculares de Contracción Lenta/metabolismo , Fosforilación , Conejos , Canal Liberador de Calcio Receptor de Rianodina/análisis , Canal Liberador de Calcio Receptor de Rianodina/biosíntesis , Retículo Sarcoplasmático/metabolismoRESUMEN
BAY-k 8644, a nifedipine analogue, promotes Ca2+ influx into excitable cells via plasma membrane voltage-sensitive Ca2+ channels. We report here that sarcoplasmic reticulum (SR) Ca2+ release channels are insensitive to BAY-k 8644, as studied in highly purified isolated fractions and in chemically skinned fibers of rabbit skeletal muscle. This result suggests that a subcellular heterogeneity exists among Ca2+ channels, at least with respect to drug-receptor sites. In the course of this study, however we found that BAY-k 8644 reversibly inhibits the SR Ca2+ pump, i.e., it decreases Ca2+ influx into the SR lumen, although at concentrations (IC50 = 3-5 X 10(-5) M) much higher than those effective on voltage-sensitive Ca2+ channels.
Asunto(s)
Calcio/metabolismo , Músculos/metabolismo , Nifedipino/análogos & derivados , Retículo Sarcoplasmático/metabolismo , Ácido 3-piridinacarboxílico, 1,4-dihidro-2,6-dimetil-5-nitro-4-(2-(trifluorometil)fenil)-, Éster Metílico , Animales , ATPasas Transportadoras de Calcio/metabolismo , Cinética , Masculino , Nifedipino/farmacología , Conejos , Retículo Sarcoplasmático/efectos de los fármacosRESUMEN
Muscle biopsies from two sporadic cases of congenital nemaline myopathy were examined for myosin heavy chain composition. Electrophoresis of congenital nemaline myopathy (CNM) muscle myosin in SDS-5% polyacrylamide gels gave rise to a single heavy chain band, with a migration rate and antigenic properties identical to that of the adult slow form, as demonstrated by Western blot techniques and by using specific antibody. Immunofluorescent studies indicate that CNM muscle fibers, including the most severely atrophic fibers, are homogeneous with respect to myosin heavy chain composition.
Asunto(s)
Enfermedades Musculares/metabolismo , Miosinas/análisis , Adulto , Anciano , Preescolar , Electroforesis en Gel de Poliacrilamida , Femenino , Humanos , Masculino , Persona de Mediana Edad , Enfermedades Musculares/congénitoRESUMEN
Muscle biopsies from two familial and one sporadic case with congenital nemaline myopathy and seven healthy family members were examined for myosin composition. Myosin was characterized with respect to light chain (LC) composition by one-dimensional and two-dimensional electrophoresis, and by immunologic methods (enzyme-linked immunosorbent assay [ELISA]), using specific antibody for rabbit fast myosin LCl (LC1F). Type I fiber predominance was associated with the substitution of a hybrid, predominantly "slow" to a virtually pure "slow" myosin LC pattern for the "mixed" pattern found with myosin of normal muscle. Muscle myosin from the relatives had apparently normal light chain composition.
Asunto(s)
Miosinas/metabolismo , Enfermedades Neuromusculares/genética , Adulto , Biopsia , Niño , Preescolar , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Lactante , Músculos/patología , Miosinas/genética , Enfermedades Neuromusculares/metabolismo , Polimorfismo GenéticoRESUMEN
A microplate enzyme-linked immunosorbent assay (microELISA) for the study of immunochemical relationships between rabbit myosin light chains is described. Purified individual fast-muscle myosin light chains (LC1F, LC2F and LC3F) and their respective antisera, obtained in chicken, were used. Optimal conditions for antigen concentration, antiserum dilution, substrate concentration, incubation time and reproducibility with time were established. The observed cross-reactivities between the different types of light chains associated with rabbit fast-muscle myosin confirm and extend previous results obtained by other authors using radioimmunoassay procedures. It was concluded that microELIAS may be successfully employed also to the study of macromolecule cross-reactivities.
Asunto(s)
Cadenas Ligeras de Inmunoglobulina , Miosinas/inmunología , Animales , Especificidad de Anticuerpos , Antígenos , Fenómenos Químicos , Química , Pollos , Reacciones Cruzadas , Ensayo de Inmunoadsorción Enzimática , Epítopos , Sueros Inmunes/farmacología , Conejos , Factores de TiempoRESUMEN
In this study we investigated the sarcoplasmic reticulum (SR), alongside myofibrillar phenotype, in muscle samples from five Myotonic Dystrophy (DM) patients and five control individuals. DM muscles exhibited as a common feature, a decrease in the slow isoform of myosin heavy chain (MHC) and of troponin C in myofibrils. We observed a match between myofibrillar changes and changes in SR membrane markers specific to fiber type, i.e. the fast (SERCA1) Ca(2+)-ATPase isoform increased concomitantly with a decrease of protein phospholamban (PLB), which in native SR membranes colocalizes with the slow (SERCA2a) SR Ca(2+)-ATPase, and regulates its activity depending on phosphorylation by protein kinases. Our results outline a cellular process selectively affecting slow-twitch fibers, and non-degenerative in nature, since neither the total number of Ca(2+)-pumps or of ryanodine receptor/Ca(2+)-release channels, or their ratio to the dihydropyridine receptor/voltage sensor in junctional transverse tubules, were found to be significantly changed in DM muscle. The only documented, apparently specific molecular changes associated with this process in the SR of DM muscle, are the defective expression of the slow/cardiac isoform of Ca(2+)-binding protein calsequestrin, together with an increased phosphorylation activity of membrane-bound 60 kDa Ca(2+)-calmodulin (CaM) dependent protein kinase. Enhanced phosphorylation of PLB by membrane-bound Ca(2+)-CaM protein kinase also appeared to be most pronounced in biopsy from a patient with a very high CTG expansion, as was the overall 'slow-to-fast' transformation of the same muscle biopsy. Animal studies showed that endogenous Ca(2+)-CaM protein kinase exerts a dual activatory role on SERCA2a SR Ca(2+)-ATPase, i.e. either by direct phosphorylation of the Ca(2+)-ATPase protein, or mediated by phosphorylation of PLB. Our results seem to be consistent with a maturational-related abnormality and/or with altered modulatory mechanisms of SR Ca(2+)-transport in DM slow-twitch muscle fibers.
Asunto(s)
Músculo Esquelético/patología , Distrofias Musculares/genética , Distrofias Musculares/patología , Retículo Sarcoplasmático/patología , Adolescente , Adulto , Anciano , Western Blotting , ATPasa de Ca(2+) y Mg(2+)/metabolismo , Mapeo Cromosómico , ADN/análisis , Electroforesis en Gel de Poliacrilamida , Humanos , Isomerismo , Masculino , Microtúbulos/metabolismo , Microtúbulos/ultraestructura , Persona de Mediana Edad , Fibras Musculares de Contracción Rápida/metabolismo , Fibras Musculares de Contracción Rápida/patología , Fibras Musculares de Contracción Rápida/ultraestructura , Fibras Musculares de Contracción Lenta/metabolismo , Fibras Musculares de Contracción Lenta/patología , Fibras Musculares de Contracción Lenta/ultraestructura , Proteínas Musculares/genética , Proteínas Musculares/metabolismo , Músculo Esquelético/enzimología , Músculo Esquelético/ultraestructura , Distrofias Musculares/enzimología , Cadenas Pesadas de Miosina/metabolismo , Proteína Quinasa de Distrofia Miotónica , Fenotipo , Proteínas Serina-Treonina Quinasas/genética , Proteínas Serina-Treonina Quinasas/metabolismo , Retículo Sarcoplasmático/enzimología , Retículo Sarcoplasmático/ultraestructura , Troponina/metabolismo , Troponina CRESUMEN
Racemic 5-hydroxy-4-oxa-endo-tricyclo[5.2.1.0(2,6)]dec-8-en-3-one and its 2-methyl analogue were resolved employing a lipase-catalyzed acetylation reaction. The latter compound thus gave access to a homochiral D-ring synthon for strigolactones. The enzymatic acetylation reaction occurred with a remarkable inversion of configuration at C-5, through which it is possible to achieve a highly efficient asymmetric synthesis of 5-acetoxy-2(5H)-furanone.
Asunto(s)
Miosinas/inmunología , Distrofia Miotónica/inmunología , Adolescente , Adulto , Ensayo de Inmunoadsorción Enzimática , Femenino , Histocitoquímica , Humanos , Masculino , Persona de Mediana Edad , Músculos/metabolismo , Músculos/patología , Miosinas/aislamiento & purificación , Distrofia Miotónica/metabolismo , Distrofia Miotónica/patologíaRESUMEN
Minor protein components of triads and of sarcoplasmic reticulum (SR) terminal cisternae (TC), i.e. 47 and 37 kDa peptides and 31-30 kDa and 26-25 kDa peptide doublets, were identified from their ability to bind 125I calsequestrin (CS) in the presence of EGTA. The CS-binding peptides are specifically associated with the junctional membrane of TC, since they could not be detected in junctional transverse tubules and in longitudinal SR fragments. The 31-30 kDa peptide doublet, exclusively, did not bind CS in the presence of Ca2+. Thus, different types of protein-protein interactions appear to be involved in selective binding of CS to junctional TC.
Asunto(s)
Calsecuestrina/metabolismo , Músculos/metabolismo , Retículo Sarcoplasmático/metabolismo , Animales , Calcio/farmacología , Membrana Celular/metabolismo , Pollos , Ácido Edético/farmacología , Concentración de Iones de Hidrógeno , Radioisótopos de Yodo , Masculino , Conejos , Especificidad de la Especie , PorcinosRESUMEN
We have investigated high-affinity ryanodine-binding sites in membrane preparations from representative fast-twitch and slow-twitch muscles of the rabbit and rat, as well as from human mixed muscle. Our results, obtained in high-ionic strength binding buffer, demonstrate extensive similarities in binding affinity for [3H]ryanodine (Kd: about 10 nM) and a two-fold to four-fold difference in membrane density of the ryanodine receptor between fast-twitch and slow-twitch muscle of the rat and rabbit, respectively. The [3H]ryanodine-pCa relationship for the Ca(2+)-activation curve of ryanodine binding was found to be similar for all mammalian muscles, as tested at 20 nM ryanodine. With 10 mM caffeine or 50 microM doxorubicin the pCa for half-maximal activation of [3H]ryanodine binding invariably shifted from an average pCa value of 6.5 to pCa 7.1-7.3. IC50 values for the inhibition of [3H]ryanodine binding by Ruthenium Red, a Ca(2+)-release channel blocker, did not differ significantly (range 0.3-1.0 microM). The Ca(2+)-dependence curve (range 1 nM-10 mM free Ca2+) that we have observed at 5 nM ryanodine, for [3H]ryanodine binding to terminal cisternae from rabbit fast-twitch, as well as slow-twitch muscle, is bell-shaped and differs from that obtained with cardiac terminal cisternae from the same species. Cardiac ryanodine receptor is also clearly distinguishable for electrophoretic mobility, Cleveland's peptide maps, and, most strikingly, for total lack of cross-reactivity with polyclonal antibody to fast skeletal RyR. By the same properties, the ryanodine receptor of fast- and slow-twitch muscle appear to be the same or a similar protein. On investigating the composition of calsequestrin in rat and human skeletal muscles, both in membrane-bound form and after purification by phenyl-Sepharose chromatography, we have been able to show that, independent of the animal species, the cardiac isoform, as characterized by the identical amino-terminal amino-acid sequence, pattern of immunoreactivity, and lack of Ca(2+)-dependent shift in mobility on SDS-PAGE, is exclusively expressed in slow-twitch fibres, together with the main fast-skeletal calsequestrin isoform. While our experimental findings strongly argue for the presence of only one population of skeletal-specific Ca(2+)-release channels in junctional terminal cisternae of mammalian fast-twitch and slow-twitch muscle, they at the same time suggest the existence of differences in calsequestrin modulation of Ca(2+)-release, depending on its isoform composition.
Asunto(s)
Canales de Calcio/análisis , Calsecuestrina/análisis , Proteínas Musculares/análisis , Músculos/química , Secuencia de Aminoácidos , Animales , Cafeína/farmacología , Calcio/metabolismo , Canales de Calcio/efectos de los fármacos , Canales de Calcio/metabolismo , Calsecuestrina/aislamiento & purificación , Doxorrubicina/farmacología , Femenino , Humanos , Masculino , Datos de Secuencia Molecular , Contracción Muscular , Proteínas Musculares/efectos de los fármacos , Proteínas Musculares/metabolismo , Músculos/fisiología , Contracción Miocárdica , Miocardio/química , Conejos , Ratas , Rianodina/metabolismo , Rianodina/farmacología , Canal Liberador de Calcio Receptor de RianodinaRESUMEN
Skeletal-muscle sarcoplasmic reticulum (SR) comprises two distinct domains, corresponding to the free membrane of longitudinal SR (LSR) and the junctional membrane region of the terminal cisternae (TC), respectively. The junctional membrane contains the ryanodine receptor (RyR)/Ca(2+)-release channel and additional minor protein components that still require biochemical investigation, in relation to excitation-contraction coupling. Recent findings suggested the involvement in this process of a 170 kDa protein [Kim, Caswell, Talvenheimo & Brandt (1990) Biochemistry 29, 9281-9289], also characterized as a phosphoprotein in junctional TC in independent studies [Chu, Submilla, Inesi, Jay & Campbell (1990) Biochemistry 29, 5899-5905]. We show that this protein is a specific substrate of exogenous cyclic AMP-dependent protein kinase, that it is exposed to the outer surface of intact TC vesicles, and that it co-localizes with the RyR to the junctional membrane. Comparative analysis of LSR and TC subfractions for the 160 kDa glycoprotein sarcalumenin, using Western-blot techniques and specific monoclonal antibodies or concanavalin A as a ligand, revealed that the distribution of this protein within the SR corresponds inversely to both that of the RyR and of the 170 kDa protein. The 170 kDa protein, like sarcalumenin, stains blue with the cationic dye Stains-All and binds 45Ca2+ on blots, but it is uniquely distinguished by its ability to bind 125I-labelled low-density lipoprotein. The similarity of these properties, as well as the pI and solubility properties, to those described for the SR protein, recently purified and cloned and named histidine-rich Ca(2+)-binding protein [HCP; Hofmann, Brown, Lee, Pathak, Anderson & Goldstein (1989) J. Biol. Chem. 264, 8260-8270], makes it very likely that our protein and HCP may indeed be identical. The protein described in the present study differs from sarcalumenin because its migration in SDS/PAGE is accelerated in the presence of Ca2+, a previously reported property of other Ca(2+)-binding proteins [leMaire, Lund, Viel, Champeil & Moller (1989) J. Biol. Chem. 265, 1111-1123], arguing for Ca(2+)-induced protein-conformational changes. Kinase-dependent phosphorylation of our protein is another distinguishing feature, which, although not previously reported for HCP, is consistent with the presence of potential serine/threonine phosphorylation sites in the middle portion of the cloned HCP molecule. The finding that HCP, contrary to early views, selectively binds to the cytoplasmic side of the junctional membrane, together with its newly characterized properties, seem to provide new clues as to a possible role in electromechanical coupling and/or Ca2+ release.