RESUMEN
This work aimed to investigate the reaction of fusel oil (FO) with pressurized water in a continuous flow reactor, in order to verify the effect of operating conditions (temperature and alcohol to water ratio) on the formation of reaction products, as well as to potentiate the antimicrobial activity of FO. The characterization of the FO was performed by high resolution mass spectrometry (ESI-TOF) and by a chromatograph coupled to mass spectrometry (GC-MS), and the reaction products were characterized by ESI-TOF and evaluated for antifungal potential. From the results, it was verified that the FO contained 70.58 wt% of isoamyl alcohol and was formed mainly by the organic functions alcohols, aldehydes, ketones and lipids. The reaction mechanisms that prevailed during the reactions conducted in subcritical and supercritical states were dehydration and reduction, respectively, making it possible to identify pyrazine derivatives compounds in the reaction products. The fungus Irpex lacteus showed greater resistance under the application of reaction products, and the products obtained at 300 °C and 400 °C showed an inhibition percentage of 96.07% to Schizophyllum commune and 96.50% to Trametes versicolor, respectively. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03429-3.