Contractile Smooth Muscle and Active Stress Generation in Porcine Common Carotids.

The mechanical response of intact blood vessels to applied loads can be delineated into passive and active components using an isometric decomposition approach. Whereas the passive response is due predominantly to the extracellular matrix (ECM) proteins and amorphous ground substance, the active response depends on the presence of smooth muscle cells (SMCs) and the contractile machinery activated within those cells. To better understand determinants of active stress generation within the vascular wall, we subjected porcine common carotid arteries (CCAs) to biaxial inflation-extension testing under maximally contracted or passive SMC conditions and semiquantitatively measured two known markers of the contractile SMC phenotype: smoothelin and smooth muscle-myosin heavy chain (SM-MHC). Using isometric decomposition and established constitutive models, an intuitive but novel correlation between the magnitude of active stress generation and the relative abundance of smoothelin and SM-MHC emerged. Our results reiterate the importance of stretch-dependent active stress generation to the total mechanical response. Overall these findings can be used to decouple the mechanical contribution of SMCs from the ECM and is therefore a powerful tool in the analysis of disease states and potential therapies where both constituent are altered.

Evaluation of head impact exposure measured from youth football game plays.

OBJECTIVE: There is a growing body of literature informing efforts to improve the safety of football; however, research relating on-field activity to head impacts in youth football is limited. Therefore, the objective of this study was to compare head impact exposure (HIE) measured in game plays among 3 youth football teams. METHODS: Head impact and video data were collected from athletes (ages 10-13 years) participating on 3 youth football teams. Video analysis was performed to verify head impacts and assign each to a specific play type. Each play was categorized as a down, punt, kickoff, field goal, or false start. Kickoffs and punts were classified as special teams. Downs were classified as running, passing, or other. HIE was quantified by play type in terms of mean, median, and 95th percentile linear and rotational acceleration. Mixed-effects models were used to assess differences in acceleration among play types. Contact occurring on special teams plays was evaluated using a standardized video abstraction form. RESULTS: A total of 3003 head impacts over 27.5 games were analyzed and paired with detailed video coding of plays. Most head impacts were attributed to running (79.6%), followed by passing (14.0%), and special teams (6.4%) plays. The 95th percentile linear acceleration measured during each play type was 52.6g, 50.7g, and 65.5g, respectively. Special teams had significantly greater mean linear acceleration than running and passing plays (both p = 0.03). The most common kick result on special teams was a deep kick, of which 85% were attempted to be returned. No special teams plays resulted in a touchback, and one resulted in a fair catch. One-third of all special teams plays and 92% of all nonreturned kicks resulted in athletes diving toward the ball. CONCLUSIONS: The results demonstrate a trend toward higher head impact magnitudes on special teams than for running and passing plays, but a greater number of impacts were measured during running plays. Deep kicks were most common on special teams, and many returned and nonreturned kicks resulted in athletes diving toward the ball. These results support policy changes to youth special teams plays, including modifying the yard line the ball is kicked from and coaching proper return technique. Further investigation into biomechanical exposure measured during game impact scenarios is needed to inform policy relevant to the youth level.