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Planta Med ; 83(18): 1420-1430, 2017 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-28651291

RESUMEN

Precise, species-level identification of plants in foods and dietary supplements is difficult. While the use of DNA barcoding regions (short regions of DNA with diagnostic utility) has been effective for many inquiries, it is not always a robust approach for closely related species, especially in highly processed products. The use of fully sequenced chloroplast genomes, as an alternative to short diagnostic barcoding regions, has demonstrated utility for closely related species. The U. S. Food and Drug Administration (FDA) has also developed species-specific DNA-based assays targeting plant species of interest by utilizing chloroplast genome sequences. Here, we introduce a repository of complete chloroplast genome sequences called GenomeTrakrCP, which will be publicly available at the National Center for Biotechnology Information (NCBI). Target species for inclusion are plants found in foods and dietary supplements, toxin producers, common contaminants and adulterants, and their close relatives. Publicly available data will include annotated assemblies, raw sequencing data, and voucher information with each NCBI accession associated with an authenticated reference herbarium specimen. To date, 40 complete chloroplast genomes have been deposited in GenomeTrakrCP (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA325670/), and this will be expanded in the future.


Asunto(s)
Bases de Datos de Ácidos Nucleicos/normas , Genoma del Cloroplasto/genética , Plantas/clasificación , Código de Barras del ADN Taxonómico , ADN de Cloroplastos/química , ADN de Cloroplastos/genética , Anotación de Secuencia Molecular , Hojas de la Planta/clasificación , Hojas de la Planta/genética , Plantas/genética , Estándares de Referencia , Especificidad de la Especie , Estados Unidos , United States Food and Drug Administration
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