Effect of short training on vaginal fluid microscopy (wet mount) learning.

OBJECTIVE: Is it feasible to learn the basics of wet mount microscopy of vaginal fluid in 10 hours? MATERIALS AND METHODS: This is a pilot project wherein 6 students with different grades of education were invited for being tested on their ability to read wet mount microscopic slides before and after 10 hours of hands-on training. Microscopy was performed according to a standard protocol (Femicare, Tienen, Belgium). Before and after training, all students had to evaluate a different set of 50 digital slides. Different diagnoses and microscopic patterns had to be scored. κ indices were calculated compared with the expert reading. RESULTS: All readers improved their mean scores significantly, especially for the most important types of altered flora (p < .0001). The mean increase in reading concordance (κ from 0.64 to 0.75) of 1 student with a solid previous experience with microscopy did not reach statistical significance, but the remaining 5 students all improved their scores from poor performance (all κ < 0.20) to moderate (κ = 0.53, n = 1) to good (κ > 0.61, n = 4) concordance. Reading quality improved and reached fair to good concordance on all microscopic items studied, except for the detection of parabasal cells and cytolytic flora. CONCLUSIONS: Although further improvement is still possible, a short training course of 10 hours enables vast improvement on wet mount microscopy accuracy and results in fair to good concordance of the most important variables of the vaginal flora compared to a reference reader.

In situ Reconstruction with Autologous Veins for the Treatment of Infected Abdominal Endografts: Single Center Experience.

Background: Vascular graft infection is a feared complication with high mortality and morbidity rates. Complete excision with in situ repair is recommended. We report our experience with patients suffering of abdominal aortic endograft infection undergoing excision and in situ reconstruction with autologous vein. Patients and Methods: All patients who underwent excision of an abdominal aortic endograft and in situ reconstruction with autologous superficial femoral veins between April 2005 and June 2021 were retrospectively reviewed. Primary outcome measures were mortality and reinfection. Secondary outcome measure was patient morbidity. Results: Fifteen patients (14 male; 93%) were included. Twenty percent of the index procedures (N = 3) were performed at our hospital, 80% (N = 12) were referred patients. Three aorto-enteric fistulae were seen. Staphylococci and enterococci were the most common pathogens (N = 8; 53%). In two out of six patients (33%) with an endograft with suprarenal fixation, the suprarenal fixation stent was left in situ. 30-day mortality rate was 6.6% (N = 1). Median follow-up time was 12 months (range 0-85). During follow-up, no reinfection was seen. Serious morbidity was witnessed in 2 patients (sepsis due to bowel leakage (N = 1), pneumonia (N = 2), hemodialysis (N = 1)). Eventration was the most common late morbidity observed (N = 5). Conclusions: Surgical treatment of vascular abdominal endograft infection by in situ reconstruction with autologous deep vein is a challenging procedure. If a multidisciplinary approach is applied and patients are centralized in experienced centers, acceptable mortality and morbidity rates can be achieved.

Eagle Syndrome: A Unique Cause of Carotid Bleeding.

Eagle syndrome is a rare aggregate of symptoms caused by an elongated styloid process. We present the unique case of bilateral vascular Eagle syndrome in a patient who experienced a unilateral acute swelling due to bleeding at the level of the right internal carotid artery. This complication has never been described before. (Level of Difficulty: Advanced.).

Screening for abnormal vaginal microflora by self-assessed vaginal pH does not enable detection of sexually transmitted infections in Ugandan women.

OBJECTIVE: Is self-assessed vaginal pH measurement to detect abnormal vaginal bacterial microflora (AVF) an adequate prescreening method for detection of genital sexually transmitted infections (STIs)? MATERIALS AND METHODS: A total of 360 Ugandan women tested themselves with a gloved finger and a pH color strip. PCR for bacterial vaginosis (BV)-associated bacteria was tested by PCR for Mycoplasma hominis, Ureaplasma urealyticum, and/or Atopobium vaginae, while the STIs were diagnosed by positive PCR for Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and/or Trichomonas vaginalis. RESULTS: A strong correlation was found between self-assessed pH values and BV-associated bacteria (P<0.0001), but not with STIs, not as single infections, nor in general. CONCLUSION: Self-measured vaginal pH correlated well with markers of high-risk microflora types such as BV or aerobic vaginitis, but not with STIs. Hence, in a screening program addressing AVF in low-resource countries, extra specific tests are required to exclude STIs.