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1.
Biol Sport ; 39(3): 751-763, 2022 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-35959341

RESUMEN

With the recent growth in female sport, practitioners need to be able to provide specific support to female athletes to ensure their sleep, health and athletic performance are optimised. Examine the patterns, duration and quality of sleep among elite female athletes, and consider the impact of situational challenges and their effects on the sleep of elite female athletes. Data was located through a search of SPORTDiscus, MEDLINE and Scopus from inception up to May 2021. Studies needed to be peer-reviewed research reporting quantitative sleep outcomes for female athletes ≥ 18 years of age and competing at a predefined elite level. A meta-analysis was performed on habitual sleep outcomes (e.g. total sleep time [TST] and sleep efficiency [SE]) measured with actigraphy. A total of 38 studies were included. Meta-analysis showed habitual TST (n = 14) was 7.8 h [7.4, 8.2] (mean [95% CI]), and SE was 86.7% [84.7, 88.6], with high variability among studies (I2 = 97.8-98.2%). Subjective sleep complaints are common before a competition, as do post-training sleep disturbances (63% studies report TST decrease), and post-competition sleep disturbances (75% studies report TST decrease). Female athletes achieve satisfactory objective sleep quantity and quality during habitual periods, but experience sleep disturbances pre- and post-situational challenges. There is high variability of objective sleep outcomes, demonstrating the individual nature of habitual female athlete sleep. Overall, future research must focus on optimising the sleep appraisal methods and creating high-quality study designs in a broader number of sports.

2.
Immunology ; 164(3): 587-601, 2021 11.
Artículo en Inglés | MEDLINE | ID: mdl-34287854

RESUMEN

Sepsis is a life-threatening condition involving a dysregulated immune response to infectious agents that cause injury to host tissues and organs. Current treatments are limited to early administration of antibiotics and supportive care. While appealing, the strategy of targeted inhibition of individual molecules in the inflammatory cascade has not proved beneficial. Non-targeted, systemic immunosuppression with steroids has shown limited efficacy and raises concern for secondary infection. Iminosugars are a class of small molecule glycomimetics with distinct inhibition profiles for glycan processing enzymes based on stereochemistry. Inhibition of host endoplasmic reticulum resident glycoprotein processing enzymes has demonstrated efficacy as a broad-spectrum antiviral strategy, but limited consideration has been given to the effects on host glycoprotein production and consequent disruption of signalling cascades. This work demonstrates that iminosugars inhibit dengue virus, bacterial lipopolysaccharide and fungal antigen-stimulated cytokine responses in human macrophages. In spite of decreased inflammatory mediator production, viral replication is suppressed in the presence of iminosugar. Transcriptome analysis reveals the key interaction of pathogen-induced endoplasmic reticulum stress, the resulting unfolded protein response and inflammation. Our work shows that iminosugars modulate these interactions. Based on these findings, we propose a new therapeutic role for iminosugars as treatment for sepsis-related inflammatory disorders associated with excess cytokine secretion.


Asunto(s)
1-Desoxinojirimicina/análogos & derivados , Antiinflamatorios/farmacología , Citocinas/metabolismo , Sepsis/tratamiento farmacológico , Respuesta de Proteína Desplegada/efectos de los fármacos , 1-Desoxinojirimicina/farmacología , 1-Desoxinojirimicina/uso terapéutico , Antiinflamatorios/uso terapéutico , Antígenos Fúngicos/inmunología , Células Cultivadas , Virus del Dengue/inmunología , Retículo Endoplásmico/efectos de los fármacos , Retículo Endoplásmico/inmunología , Retículo Endoplásmico/metabolismo , Interacciones Huésped-Patógeno/efectos de los fármacos , Interacciones Huésped-Patógeno/inmunología , Humanos , Inflamación/tratamiento farmacológico , Inflamación/inmunología , Inflamación/microbiología , Lipopolisacáridos/inmunología , Macrófagos , Cultivo Primario de Células , Sepsis/inmunología , Sepsis/microbiología , Receptor Toll-Like 4/metabolismo , Respuesta de Proteína Desplegada/inmunología
3.
J Strength Cond Res ; 35(6): 1620-1627, 2021 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-30741866

RESUMEN

ABSTRACT: Jones, MJ, Dawson, B, Eastwood, PR, Halson, SL, Miller, J, Murray, K, Dunican, IC, Landers, GJ, and Peeling, P. Influence of electronic devices on sleep and cognitive performance during athlete training camps. J Strength Cond Res 35(6): 1620-1627, 2021-This study investigated the effects of removing athletes' electronic devices in the evening on sleep and performance during training camps. Water polo athletes (n = 26) attending a 7-night training camp (study 1) and triathletes (n = 23) attending a 4-night training camp (study 2) were randomly allocated to a no-device group (no electronic devices could be used after dinner or overnight; ND) or control group (unrestricted electronic device use; CON). Sleep was monitored through wrist actigraphy. The ND group completed a questionnaire measuring anxiety related to being unable to use electronic devices ("nomophobia"). Triathletes also completed a psychomotor vigilance test (PVT) at the start and end of camp. Water polo ND athletes went to bed earlier and spent longer time in bed than CON on the first night, but not on other nights. In triathletes, sleep quantity was not different between groups on any night. No statistically significant differences were observed for changes in nomophobia from the first to the last night of camp. No differences in PVT performance were observed between ND and CON triathletes. In conclusion, removal of evening electronic devices does not improve sleep quantity or cognitive performance in athletes during short-duration (4-7 nights) training camps.


Asunto(s)
Atletas , Cognición/fisiología , Sueño , Actigrafía , Electrónica , Humanos , Sueño/fisiología
4.
Anesth Analg ; 129(1): 306-310, 2019 07.
Artículo en Inglés | MEDLINE | ID: mdl-30418237

RESUMEN

Despite increasing representation in medicine, women continue to be a minority in academic practice and leadership, especially in male-dominated fields like anesthesiology. Differences in compensation and participation in leadership may represent barriers to career advancement for women in anesthesiology. Key factors for promotion in academic anesthesiology are research, funding, and publication. As such, designation as a first or senior author on a publication in a professional journal may act as currency for promotion. Here, we examine the prevalence of female first and senior authorship of original research articles published in Anesthesiology and Anesthesia & Analgesia during the years 2002, 2007, 2012, and 2017. Other manuscript characteristics assessed in relation to author gender included study type, subspecialty topic, and total number of authors. Of 2600 manuscripts studied, analysis of authorship by year demonstrated an increase in female first authorship, senior authorship, and editorial board membership by 10%, 9%, and 6%, respectively. Women made up a higher percentage of first authors on manuscripts with female senior authors. More nonexperimental studies had female senior authors than experimental studies. Female first authors had greater representation in the subspecialties of neuroanesthesia, obstetrical anesthesia, pain management, and pediatric anesthesia. Median number of authors was unrelated to senior author gender. This study shows increasing female first and senior authorship, as well as editorial board composition in 2 popular, high-impact anesthesiology journals. Recognizing gender-based trends in publications is important to develop strategies for the recruitment, retention, and advancement of women in anesthesiology.


Asunto(s)
Anestesiología/tendencias , Autoria , Investigación Biomédica/tendencias , Publicaciones Periódicas como Asunto/tendencias , Sexismo/tendencias , Bibliometría , Femenino , Humanos , Masculino , Factores Sexuales
5.
Anesth Analg ; 129(3): e77-e82, 2019 09.
Artículo en Inglés | MEDLINE | ID: mdl-31425212

RESUMEN

This retrospective observational case series conducted at 2 large academic centers over a 4-year period consists of 15 cases of profound hypotension in surgical patients immediately after initiation of the Belmont Fluid Management System for rapid transfusion of blood products. Halting the infusion and administering vasoactive agents led to resolution of hypotension. Repeat transfusion with the Belmont system resulted in repeat hypotension unless counteracted with vasopressors. No etiology was elucidated. This represents the largest documented association of acute hypotensive transfusion reaction with any rapid infusion system in surgical patients.


Asunto(s)
Fluidoterapia/efectos adversos , Complicaciones Intraoperatorias/diagnóstico , Complicaciones Intraoperatorias/etiología , Reacción a la Transfusión/diagnóstico , Reacción a la Transfusión/etiología , Adulto , Anciano , Transfusión Sanguínea/tendencias , Femenino , Fluidoterapia/tendencias , Humanos , Complicaciones Intraoperatorias/terapia , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Reacción a la Transfusión/terapia
6.
J Sports Sci ; 37(8): 864-870, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30326782

RESUMEN

The present study aimed to investigate pre-sleep behaviours (including evening electronic device use) and sleep quantity in well-trained athletes. Seventy well-trained athletes (44 females, 26 males) aged 21 ± 4 y from a range of team and individual sports were asked to complete an online sleep diary for 7 days. The sleep diary included questions about pre-sleep behaviours (e.g. napping, caffeine intake), electronic device use in the 2 h prior to bedtime (e.g. type of device and duration of use) and sleep (e.g. time in bed, sleep onset latency). On average, athletes spent 8:20 ± 1:21 h in bed each night. Associations between age, time in bed and sleepiness suggested that younger athletes spent more time in bed (B = -0.05, p = 0.001) but felt sleepier (r = -0.32, p < 0.01) than older athletes. On average, athletes mostly used electronic devices for 0-30 min prior to sleep. The use of multiple devices in the evening was associated with more perceived difficulty in falling asleep (B = 0.22, p = 0.03), but no associations existed with other sleep variables. In summary, younger athletes may require later start times or improved sleep quality to resolve excessive sleepiness.


Asunto(s)
Atletas/psicología , Hábitos , Microcomputadores/estadística & datos numéricos , Sueño , Adulto , Factores de Edad , Utilización de Equipos y Suministros , Femenino , Humanos , Masculino , Factores de Tiempo , Adulto Joven
7.
Cell Microbiol ; 19(5)2017 05.
Artículo en Inglés | MEDLINE | ID: mdl-28207988

RESUMEN

Symptomatic dengue virus (DENV) infections range from mild fever to severe haemorrhagic disease and death. Host-viral interactions play a significant role in deciding the fate of the infection. The unfolded protein response (UPR) is a prosurvival cellular reaction induced in response to DENV-mediated endoplasmic reticulum stress. The UPR has complex interactions with the cellular autophagy machinery, apoptosis, and innate immunity. DENV has evolved to manipulate the UPR to facilitate its replication and to evade host immunity. Our knowledge of this intertwined network of events is continuously developing. A better understanding of the UPR mediated antiviral and proviral effects will shed light on dengue disease pathogenesis and may help development of anti-DENV therapeutics. This review summarizes the role of the UPR in viral replication, autophagy, and DENV-induced inflammation to describe how a host response contributes to DENV pathogenesis.


Asunto(s)
Virus del Dengue/fisiología , Dengue/inmunología , Respuesta de Proteína Desplegada/inmunología , Animales , Apoptosis , Autofagia , Dengue/patología , Interacciones Huésped-Patógeno , Humanos , Inmunidad Innata , Replicación Viral
8.
Adv Exp Med Biol ; 1062: 277-301, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29845540

RESUMEN

The antiviral mechanism of action of iminosugars against many enveloped viruses, including dengue virus (DENV), HIV, influenza and hepatitis C virus, is believed to be mediated by inducing misfolding of viral N-linked glycoproteins through inhibition of host endoplasmic reticulum-resident α-glucosidase enzymes. This leads to reduced secretion and/or infectivity of virions and hence lower viral titres, both in vitro and in vivo. Free oligosaccharide analysis from iminosugar-treated cells shows that antiviral activity correlates with production of mono- and tri-glucosylated sugars, indicative of inhibition of ER α-glucosidases. We demonstrate that glucose-mimicking iminosugars inhibit isolated glycoprotein and glycolipid processing enzymes and that this inhibition also occurs in primary cells treated with these drugs. Galactose-mimicking iminosugars that have been tested do not inhibit glycoprotein processing but do inhibit glycolipid processing, and are not antiviral against DENV. By comparison, the antiviral activity of glucose-mimetic iminosugars that inhibit endoplasmic reticulum-resident α-glucosidases, but not glycolipid processing, demonstrates that inhibition of α-glucosidases is responsible for iminosugar antiviral activity against DENV. This monograph will review the investigations of many researchers into the mechanisms of action of iminosugars and the contribution of our current understanding of these mechanisms for optimising clinical delivery of iminosugars. The effects of iminosugars on enzymes other than glucosidases, the induction of ER stress and viral receptors will be also put into context. Data suggest that inhibition of α-glucosidases results in inhibited release of virus and is the primary antiviral mechanism of action of iminosugars against DENV.


Asunto(s)
Virus del Dengue/fisiología , Dengue/inmunología , Azúcares/inmunología , Animales , Dengue/enzimología , Dengue/genética , Dengue/virología , Virus del Dengue/genética , Retículo Endoplásmico/enzimología , Retículo Endoplásmico/genética , Retículo Endoplásmico/inmunología , Retículo Endoplásmico/virología , Humanos , alfa-Glucosidasas/genética , alfa-Glucosidasas/inmunología
9.
J Sports Sci ; 36(9): 1044-1053, 2018 May.
Artículo en Inglés | MEDLINE | ID: mdl-28703035

RESUMEN

This study examined the influence of body composition on temperature and blood flow responses to post-exercise cold water immersion (CWI), hot water immersion (HWI) and control (CON). Twenty-seven male participants were stratified into three groups: 1) low mass and low fat (LM-LF); 2) high mass and low fat (HM-LF); or 3) high mass and high fat (HM-HF). Experimental trials involved a standardised bout of cycling, maintained until core temperature reached 38.5°C. Participants subsequently completed one of three 15-min recovery interventions (CWI, HWI, or CON). Core, skin and muscle temperatures, and limb blood flow were recorded at baseline, post-exercise, and every 30 min following recovery for 240 min. During CON and HWI there were no differences in core or muscle temperature between body composition groups. The rate of fall in core temperature following CWI was greater in the LM-LF (0.03 ± 0.01°C/min) group compared to the HM-HF (0.01 ± 0.001°C/min) group (P = 0.002). Muscle temperature decreased to a greater extent during CWI in the LM-LF and HM-LF groups (8.6 ± 3.0°C) compared with HM-HF (5.1 ± 2.0°C, P < 0.05). Blood flow responses did not differ between groups. Differences in body composition alter the thermal response to post-exercise CWI, which may explain some of the variance in the responses to CWI recovery.


Asunto(s)
Ciclismo/fisiología , Distribución de la Grasa Corporal , Índice de Masa Corporal , Regulación de la Temperatura Corporal/fisiología , Hidroterapia , Músculo Esquelético/irrigación sanguínea , Flujo Sanguíneo Regional , Adulto , Presión Sanguínea/fisiología , Superficie Corporal , Frío , Estudios Cruzados , Frecuencia Cardíaca/fisiología , Calor , Humanos , Inmersión , Extremidad Inferior/irrigación sanguínea , Extremidad Superior/irrigación sanguínea
10.
J Sports Sci ; 36(13): 1476-1484, 2018 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-29099652

RESUMEN

Currently, very little is known about the impact of short- or long-haul air travel on the sleep and wellbeing of wheelchair basketball athletes. Eleven national wheelchair basketball athletes wore actigraphy monitors prior, during, and after air travel to the United Kingdom. Upon arrival, participants rated their subjective jet-lag, fatigue, and vigor. Individuals traveled to the United Kingdom from different locations in Australia, the United States, and Europe and were categorised according to travel length [LONG (up to 30.2 h) or SHORT (up to 6.5 h)]. Linear mixed models determined effects of travel length on sleep and subjective ratings of jet-lag, fatigue, and vigor. During competition, subjective fatigue and jet-lag were substantially higher (ES = 0.73; ±0.77) and (ES = 0.57; ±0.60), subjective vigor was lower (ES = 1.94; ±0.72), and get-up time was earlier (ES = 0.57; ±0.60) for LONG when compared to SHORT. Travelling greater distances by airplane had a larger effect on subjective ratings of jet-lag, fatigue and vigor, rather than sleep. Irrespective of travel group, sleep and subjective responses were compromised, reflecting the travel requirements, competition-mediated influences, and/or due to a change in environment.


Asunto(s)
Rendimiento Atlético/fisiología , Baloncesto/fisiología , Síndrome Jet Lag/fisiopatología , Sueño/fisiología , Viaje , Silla de Ruedas , Adulto , Humanos , Factores de Tiempo
11.
J Sports Sci ; 36(2): 162-170, 2018 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-28282750

RESUMEN

The aim of the present study was to investigate the influence of different types of tasks performed with or without an electronic device (tablet) on pre-sleep alertness, subsequent sleep quality and next-day athletic performance. Eight highly trained netball players attended a sleep laboratory for pre-sleep testing, polysomnographic sleep monitoring and next-day physical performance testing on 5 separate occasions (1 familiarisation and 4 experimental sessions). For 2 h prior to bedtime, athletes completed cognitively stimulating tasks (puzzles) or passive tasks (reading) with or without a tablet. Sleepiness tended to be greater after reading compared to completing puzzles without a tablet (d = 0.80), but not with a tablet. Melatonin concentration increased more so after reading compared to completing puzzles on a tablet (P = 0.02). There were no significant differences in sleep quality or quantity or next-day athletic performance between any of the conditions. These data suggest that using a tablet for 2 h prior to sleep does not negatively affect subsequent sleep or next-day performance in athletes.


Asunto(s)
Rendimiento Atlético , Atención , Microcomputadores/estadística & datos numéricos , Sueño , Adolescente , Temperatura Corporal , Oído Medio/fisiología , Femenino , Humanos , Hidrocortisona/metabolismo , Melatonina/metabolismo , Salvia/metabolismo , Análisis y Desempeño de Tareas
12.
J Sports Sci ; 36(18): 2033-2037, 2018 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-29384434

RESUMEN

The 10-min Psychomotor Vigilance Test (PVT-10) is regarded as the gold-standard for assessing vigilant attention following sleep loss; however, other studies have investigated whether shorter versions of the test elicit similar results to the PVT-10. The present study compared the PVT-10 with 3-min (PVT-3) and 5-min (PVT-5) versions of the test in elite female basketball players. Athletes performed all three tests in the morning and evening for seven consecutive days. Response speed (mean reciprocal reaction time; mean 1/RT), number of errors and number of lapses were determined for each test and time point. The PVT-3 elicited significantly faster response speeds than the other two tests (p < 0.01), while the PVT-5 and PVT-10 were not different. The PVT-10 resulted in more lapses than the PVT-5, followed by the PVT-3, with all tests being significantly different to each other (p < 0.01). In conclusion, while the PVT-5 and PVT-10 were generally similar for response speed, the PVT-3 did not produce results comparable with the PVT-10 for response speed, lapses or errors, and should therefore not be used interchangeably. Further research is required to determine whether the shorter tests are a suitable replacement for the PVT-10 in professional basketball players.


Asunto(s)
Baloncesto/fisiología , Baloncesto/psicología , Pruebas Neuropsicológicas , Desempeño Psicomotor/fisiología , Femenino , Humanos , Tiempo de Reacción/fisiología , Sueño/fisiología , Factores de Tiempo , Adulto Joven
13.
J Sports Sci Med ; 17(3): 417-425, 2018 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-30116115

RESUMEN

The implications of impact-induced muscle damage (IIMD) that results from participation in contact-sport are not well understood. The purpose of the present study was to implement a novel method of generating IIMD and characterise the implications of this on perceptual, biochemical and exercise performance parameters. Eighteen male recreational contact-sport athletes completed a single-group time series with measures assessed at baseline (PRE) and immediately following (POST) an IIMD protocol, with repeat testing 24, 48, and 72 h following the IIMD protocol. Biochemical indices of muscle damage (myoglobin [Mb]) and inflammation (high-sensitivity C-reactive protein [hs-CRP]), 15 m sprint performance, squat jump peak power (SJ-PP), and perceived soreness were compared to PRE using a one-way (time) repeated measures ANOVA with post-hoc t tests. Speed over 5 and 15 m were impaired for 48 h (7.5 ± 4%, p < 0.01) and SJ-PP was impaired for 48 h following the IIMD protocol (9.5 ±3 %, p < 0.01). Subjective soreness was elevated from baseline for 72 h (P < 0.01) following the IIMD protocol. No change in [CRP] or [Mb] was observed (p > 0.01). IIMD resulted in impaired ability to produce power and speed, whilst negatively influencing perceived soreness. These changes were most pronounced in the 48 h following the IIMD protocol. No change in muscle damage or inflammation indices were observed, primarily due to the highly variable response. Thus, the experimental protocol used in the present study may be used as a model to further investigate other aspects of IIMD.


Asunto(s)
Rendimiento Atlético/fisiología , Inflamación/sangre , Músculo Esquelético/lesiones , Adulto , Atletas , Biomarcadores/sangre , Proteína C-Reactiva/análisis , Humanos , Masculino , Mialgia/fisiopatología , Mioglobina/sangre , Dimensión del Dolor , Deportes , Adulto Joven
14.
Crit Rev Microbiol ; 43(5): 521-545, 2017 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-27931136

RESUMEN

Influenza virus causes three to five million severe respiratory infections per year in seasonal epidemics, and sporadic pandemics, three of which occurred in the twentieth century and are a continuing global threat. Currently licensed antivirals exclusively target the viral neuraminidase or M2 ion channel, and emerging drug resistance necessitates the development of novel therapeutics. It is believed that a host-targeted strategy may combat the development of antiviral drug resistance. To this end, a class of molecules known as iminosugars, hydroxylated carbohydrate mimics with the endocyclic oxygen atom replaced by a nitrogen atom, are being investigated for their broad-spectrum antiviral potential. The influenza virus glycoproteins, hemagglutinin and neuraminidase, are susceptible to inhibition of endoplasmic reticulum α-glucosidases by certain iminosugars, leading to reduced virion production or infectivity, demonstrated by in vitro and in vivo studies. In some experiments, viral strain-specific effects are observed. Iminosugars may also inhibit other host and virus targets with antiviral consequences. While investigations of anti-influenza iminosugar activities have been conducted since the 1980s, recent successes of nojirimycin derivatives have re-invigorated investigation of the therapeutic potential of iminosugars as orally available, low cytotoxicity, effective anti-influenza drugs.


Asunto(s)
1-Desoxinojirimicina/análogos & derivados , Antivirales/uso terapéutico , Inhibidores de Glicósido Hidrolasas/uso terapéutico , Gripe Humana/tratamiento farmacológico , 1-Desoxinojirimicina/uso terapéutico , Animales , Farmacorresistencia Viral , Retículo Endoplásmico/enzimología , Retículo Endoplásmico/metabolismo , Glicosilación , Glicoproteínas Hemaglutininas del Virus de la Influenza/metabolismo , Humanos , Subtipo H1N1 del Virus de la Influenza A/metabolismo , Gripe Humana/virología , Ratones , Neuraminidasa/metabolismo , alfa-Glucosidasas/metabolismo
16.
Eur J Sport Sci ; 23(8): 1509-1517, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-36939844

RESUMEN

Wearable temperature sensors offer the potential to overcome several limitations associated with current laboratory- and field-based methods for core temperature assessment; however, their ability to provide accurate data at elevated core temperatures (Tc) has been questioned. Therefore, this investigation aimed to determine the concurrent validity of a wearable temperature sensor (CORE) compared to a reference telemetric temperature pill (BodyCAP) during a team-sport heat training camp prior to the 2020 Olympic Games. Female field hockey players (n = 19) in the Australian national squad completed 4 sessions in hot conditions where their temperature was monitored via CORE and BodyCAP. Concurrent validity of the wearable CORE device was determined with reference to the ingested BodyCAP pill. Lin's Concordance Correlation Coefficients determined there was "poor" agreement between devices during all sessions. Mean bias demonstrated that CORE underestimated Tc in all sessions (-0.06°C to -0.34°C), with wide mean 95% confidence intervals (±0.35°C to ±0.56°C). Locally estimated scatterplot smoothing regression lines illustrated a non-linearity of error, with greater underestimation of Tc by the CORE device, as Tc increased. The two devices disagreed more than ±0.3°C for 41-60% of all data samples in each session. Our findings do not support the use of the CORE device as a valid alternative to telemetric temperature pills for Tc assessment, particularly during exercise in hot conditions where elevated Tc are expected.


The CORE wearable sensor is not a valid alternative to telemetric temperature pills for Tc assessment, particularly during exercise in hot conditions where elevated Tc are expected.Compared to reference Tc data provided by a validated, ingestible telemetric temperature pill, the CORE device demonstrated "poor" agreement between devices during all sessions in this investigation.There was a non-linear bias which tended to underestimate Tc to a greater extent as Tc increased (but with wide confidence intervals), with 41-60% of all data exceeding a threshold error of ±0.3°C.


Asunto(s)
Temperatura Corporal , Hockey , Femenino , Humanos , Temperatura , Calor , Australia
17.
Pathogens ; 12(3)2023 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-36986321

RESUMEN

Crimean-Congo haemorrhagic fever virus (CCHFV) is a pathogen of increasing public health concern, being a widely distributed arbovirus and the causative agent of the potentially fatal Crimean-Congo haemorrhagic fever. Hazara virus (HAZV) is a genetically and serologically related virus that has been proposed as a surrogate for antiviral and vaccine testing for CCHFV. Glycosylation analysis of HAZV has been limited; first, we confirmed for the first time the occupation of two N-glycosylation sites in the HAZV glycoprotein. Despite this, there was no apparent antiviral efficacy of a panel of iminosugars against HAZV, as determined by quantification of the total secretion and infectious virus titres produced following infection of SW13 and Vero cells. This lack of efficacy was not due to an inability of deoxynojirimycin (DNJ)-derivative iminosugars to access and inhibit endoplasmic reticulum α-glucosidases, as demonstrated by free oligosaccharide analysis in uninfected and infected SW13 and uninfected Vero cells. Even so, iminosugars may yet have potential as antivirals for CCHFV since the positions and importance of N-linked glycans may differ between the viruses, a hypothesis requiring further evaluation.

18.
Antimicrob Agents Chemother ; 56(12): 6379-86, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-23070155

RESUMEN

A key challenge faced by promising antiviral drugs, such as iminosugars, is in vivo delivery to achieve effective levels of drug without toxicity. Four iminosugars, all deoxynojirimycin (DNJ) derivatives-N-butyl DNJ (NB-DNJ), N-nonyl DNJ, N-(9-methoxynonyl) DNJ, and N-(6'-[4″-azido-2″-nitrophenylamino]hexyl)-1-DNJ (NAP-DNJ)-potently inhibited both the percentage of cells infected with dengue virus and release of infectious virus from primary human monocyte-derived macrophages, demonstrating their efficacy in primary cells. In a lethal antibody-dependent enhancement mouse model of dengue pathogenesis, free NB-DNJ significantly enhanced survival and lowered viral load in organs and serum. Liposome-mediated delivery of NB-DNJ, in comparison with free NB-DNJ, resulted in a 3-log(10) reduction in the dose of drug sufficient to enhance animal survival. The optimizing of the effective dose in this way could liberate the therapeutic potential of many cytotoxic antivirals against both dengue virus and a wide array of other viruses.


Asunto(s)
Antivirales/administración & dosificación , Antivirales/uso terapéutico , Virus del Dengue/efectos de los fármacos , Dengue/tratamiento farmacológico , Iminoazúcares/administración & dosificación , Iminoazúcares/uso terapéutico , 1-Desoxinojirimicina/administración & dosificación , 1-Desoxinojirimicina/uso terapéutico , Animales , Supervivencia Celular/efectos de los fármacos , Dengue/virología , Portadores de Fármacos , Sistemas de Liberación de Medicamentos , Dosificación de Gen , Humanos , Técnicas In Vitro , Liposomas , Macrófagos/efectos de los fármacos , Macrófagos/microbiología , Ratones , ARN Viral/biosíntesis , ARN Viral/genética , Reacción en Cadena en Tiempo Real de la Polimerasa
19.
Front Vet Sci ; 9: 1014687, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36713862

RESUMEN

Introduction: The objective of this study was to retrospectively analyze clinical data from a referral regenerative medicine practice, to investigate the efficacy of autologous mesenchymal stromal cells (MSC) in 245 dogs deemed unresponsive to conventional treatment by their referring vet. Methods: Diagnostic imaging [radiology and musculoskeletal ultrasound (MSK-US)] identified musculoskeletal pathology holistically. MSCs, produced according to current guidelines, were initially administered with PRP by targeted injection to joints and/or tendons, with a second MSC monotherapy administered 12 weeks later to dogs with severe pathology and/or previous elbow arthroscopic interventions. Dogs with lumbosacral disease received epidural MSCs with additional intravenous MSCs administered to dogs with spondylosis of the cervical, thoracic and lumbar spine. All dogs received laser therapy at 10 J/cm2 at the time of treatment and for 5 sessions thereafter. Objective outcome measures (stance analysis, range of joint motion, pressure algometry) and validated subjective outcome measures (owner reported VetMetrica HRQL™ and veterinary pain and quality of life impact scores) were used to investigate short and long-term (6-104 weeks) efficacy. Outcome data were collected at predetermined time windows (0-6, 7-12, 13-18, 19-24, 25-48, 49-78, 79-104) weeks after initial treatment. Results: There were statistically significant improvements in post compared with pre-treatment measures at all time windows in stance analysis, shoulder and hip range of motion, lumbosacral pressure algometry, and to 49-78 weeks in carpus and elbow range of motion. Improvements in 4 domains of quality of life as measured by VetMetricaTM were statistically significant, as were scores in vet-assessed pain and quality of life impact. In dogs receiving one initial treatment the mean time before a second treatment was required to maintain improvements in objective measures was 451 days. Diagnostic imaging confirmed the regenerative effects of MSCs in tendinopathies by demonstrating resolution of abnormal mineralization and restoration of normal fiber patterns. Discussion: This represents the first study using "real-world" data to show that cell-based therapies, injected into multiple areas of musculoskeletal pathology in a targeted holistic approach, resulted in rapid and profound positive effects on the patient's pain state and quality of life which was maintained with repeat treatment for up to 2 years.

20.
Int J Sports Physiol Perform ; 17(2): 234-240, 2022 02 01.
Artículo en Inglés | MEDLINE | ID: mdl-34686612

RESUMEN

PURPOSE: To compare the habitual sleep of female basketball and soccer athletes to age- and sex-matched controls and to characterize the sleep of basketball and soccer athletes at different competition locations and on the days surrounding competition. METHODS: Using an observational case-control design, 41 female participants were recruited to participate, consisting of 11 basketball athletes (mean [SD]: age = 24.1 [4.9] y), 10 soccer athletes (24.8 [6.4] y), and 20 nonathletic controls (24.2 [2.8] y). Sleep was monitored using actigraphy for four 7-day periods throughout the preseason and subsequent competition season. Generalized linear models were used to analyze the effect of group and competition situation (eg, Home or Away) on sleep. RESULTS: During habitual conditions, basketball athletes had longer sleep durations (7.4 [1.5] h) than soccer athletes (7.0 [1.2] h, P < .001) and controls (7.3 [1.2] h, P = .002). During competition, basketball and soccer athletes had longer sleep durations following home (7.7 [1.7] and 7.2 ± 1.3 h) compared with away games (6.8 [1.8] and 7.0 [1.3] h). In addition, basketballers went to bed earlier (23:49 [01:25]) and woke earlier (07:22 [01:59]) following away games compared with soccer athletes (00:10 [01:45] and 08:13 [01:45]). CONCLUSIONS: Basketballers had longer habitual sleep durations compared with soccer athletes and nonathletic controls. During competition, basketballers had earlier bed and wake times compared with soccer athletes following away games, highlighting the need for individualized sleep strategies.


Asunto(s)
Baloncesto , Fútbol , Actigrafía , Adulto , Atletas , Estudios de Casos y Controles , Femenino , Humanos , Sueño , Adulto Joven
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