Role of prokaryotic biomasses and activities in carbon and phosphorus cycles at a coastal, thermohaline front and in offshore waters (Gulf of Manfredonia, Southern Adriatic Sea).

The Western areas of the Adriatic Sea are subjected to inputs of inorganic nutrients and organic matter that can modify the trophic status of the waters and consequently, the microbiological processes involved in the carbon and phosphorus biogeochemical cycles, particularly in shallow coastal environments. To explore this topic, a survey was carried out during the spring of 2003 in a particular hydrodynamic area of the Gulf of Manfredonia, where the potential (P) and real (R) rates of four different microbial exoenzymatic activities (EEA) (α [αG] and ß glucosidases [ßG], leucine aminopeptidase [LAP], and alkaline phosphatase [AP]) as well as the P and R rates of prokaryotic heterotrophic production (PHP), AP as well as the P and R rates of PHP, primary production (PPnet), the prokaryotic and phototrophic stocks and basic hydrological parameters were examined. Three different water masses were found, with a thermohaline front (THF) being detected between the warmer and less saline coastal waters and colder and saltier offshore Adriatic waters. Under the general oligotrophic conditions of the entire Gulf, a decreasing gradient from the coastal toward the offshore areas was detected, with PHP, PPnet, stocks and EEA (αG, ßG, AP) being directly correlated with the temperature and inversely correlated with the salinity, whereas opposite relationships were observed for LAP activity. No enhancement of microbiological activities or stocks was observed at the THF. The use of P or R rates of microbiological activities, which decrease particularly for EEA, could result in discrepancies in interpreting the efficiency of several metabolic processes.

Seasonal dynamics of prokaryotic abundance and activities in relation to environmental parameters in a transitional aquatic ecosystem (Cape Peloro, Italy).

This study examines the effects of temporal changes on microbial parameters in a brackish aquatic ecosystem. To this aim, the abundances of prokaryotes and vibrios together with the rates of enzymatic hydrolysis of proteins by leucine aminopeptidase (LAP), polysaccharides by ß-glucosidase (GLU) and organic phosphates by alkaline phosphatase (AP), heterotrophic prokaryotic production (HPP), respiration (R), were seasonally investigated, during a 2-year period in the coastal area of Cape Peloro (Messina, Italy), constituted by two brackish lakes (Faro and Ganzirri). In addition, physical and chemical parameters (temperature, salinity, nutrients) and particulate organic carbon and nitrogen (POC, PN) were measured. The influence of multiple factors on prokaryotic abundances and activities was analysed. The results showed that Cape Peloro area is characterised by high seasonal variability of the microbial parameters that is higher than the spatial one. Combined changes in particulate matter and temperature (T), could explain the variability in vibrios abundance, GLU and R activities in both lakes, indicating a direct stimulation of the warm season on the heterotrophic prokaryotic metabolism. Positive correlations between T (from 13.3 to 29.6 °C) and HPP, LAP, AP, POC, PN are also observed in Ganzirri Lake. Moreover, the trophic status index and most of the microbial parameters show significant seasonal differences. This study demonstrates that vibrios abundance and microbial activities are responsive to the spatial and seasonal changes of examined area. The combined effects of temperature and trophic conditions on the microbial parameters lead us to suggest their use as potential indicators of the prokaryotic response to climate changes in temperate brackish areas.

[Promoting the quality of health surveillance of workers exposed to wood dust, with particular care to NPSC, in the territory of the Health Agency of Florence]. / Promozione della qualità della sorveglianza sanitaria dei lavoratori esposti a polvere di legno, con particolare riferimento ai TuNS, nel territorio di competenza della Azienda Sanitoria di Firenze.

Wood dust can cause occupational-related naso-sinusal cancer, characterized by a latency period of about 40 years. The Tuscany Cancer Registry estimates that cases of NPSC are from 20-25 per year into the Region (33% related to wood dust). These neoplasms are surgically treatable at early-stage and, for this reason, a rapid endoscopic diagnosis is considered to be reasonably useful for prognostic issues. We used a questionnaire to investigate nasal symptoms and NOSQ and SOLAR questionnaires to highlight respiratory/skins diseases, and a spirometry for each worker. Subjects with a working-age of more than 15 years, and those that were positive to the questionnaire and/or to the medical history were were referred to a specialist in otolaryngology. The prevalence of endoscopic positive findings--detected especially in subjects with a working age of more than 15 years--confirms the significance of the problem.

Development of a fluorescent antibody method for the detection of Enterococcus faecium and its potential for coastal aquatic environment monitoring.

A direct, microscopic fluorescent antibody method was developed to detect the occurrence of Enterococcus faecium in coastal aquatic environments and was compared with the conventional membrane filtering method. The "in situ" application of the antibody-based protocol in the analysis of water samples collected from coastal polyhaline habitats demonstrated good sensitivity and ease of implementation. Data obtained with the microscopic technique were in agreement with those obtained from culture counts. The fluorescent antibody method proved to be a rapid and reliable technique for the detection of E. faecium. The advantages and limitations intrinsic to the method are discussed, highlighting the potential of this new technique for monitoring coastal aquatic environments.

Interaction of hydrophobic polymers with model lipid bilayers.

The interaction of nanoscale synthetic materials with cell membranes is one of the key steps determining nanomaterials' toxicity. Here we use molecular simulations, with atomistic and coarse-grained resolution, to investigate the interaction of three hydrophobic polymers with model lipid membranes. Polymer nanoparticles made of polyethylene (PE), polypropylene (PP) and polystyrene with size up to 7 nm enter easily POPC lipid membranes, localizing to the membrane hydrophobic core. For all three materials, solid polymeric nanoparticles become essentially liquid within the membrane at room temperature. Still, their behavior in the membrane core is not the same: PP and PS disperse in the core of the bilayer, while PE shows a tendency to aggregate. We also examined the interaction of the polymers with heterogeneous membranes, consisting of a ternary lipid mixture exhibiting liquid-ordered/liquid-disordered phase separation. The behavior of the three polymers is markedly different: PP disfavors lipid phase separation, PS stabilizes it, and PE modifies the topology of the phase boundaries and causes cholesterol depletion from the liquid ordered phase. Our results show that different hydrophobic polymers have major effects on the properties of lipid membranes, calling for further investigations on model systems and cell membranes.

Seasonal changes on microbial metabolism and biomass in the euphotic layer of Sicilian Channel.

As a part of a wider project on fisheries ecology, several biological and environmental parameters were monitored during two oceanographic cruises (BANSIC 2012 and NOVESAR 2013) in the Sicily Channel, which connects the Western and Eastern Mediterranean basins. The prokaryotic abundances and biomass as well as hydrolysis rates on organic matter were investigated in the euphotic layer of a retention area for fish larval stages including anchovy (Engraulis encrasicolus, Linnaeus, 1758) with the aim to investigate the different biogeochemical signatures in two seasonal conditions. The environmental parameters, particulate organic carbon and nitrogen together with heterotrophic production were also measured. Results showed significant increases for most of the studied parameters with increasing temperature during summer. This had effects on the Carbon cycle and recycling of nutrients; in fact total prokaryotic abundance and biomass, as well as carbon hydrolyzed by two enzymes (Leucine aminopeptidase and ß-glucosidase), increased significantly during summer. Conversely Alkaline phosphatase activity, Chlorophyll concentration and Oxygen increased during winter. The same environmental parameters affected also the presence of fish eggs. Moreover high percentages of free enzymes (i.e., enzymes not associated with cells) were measured, accounting for percentages variable from 12 to 95 % of the total enzymatic activity, with values generally higher in summer than in winter. In this oligotrophic environment, the prokaryotic biomass was supported by the C hydrolyzed by enzymatic activities. The ratio between the hydrolyzed C and prokaryotic biomass was higher in winter than in summer, indicating that alkaline phosphatase activity contribute to an efficient incorporation of C into biomass in winter.

The prostaglandin D2 receptor CRTH2 regulates accumulation of group 2 innate lymphoid cells in the inflamed lung.

Group 2 innate lymphoid cells (ILC2s) promote type 2 cytokine-dependent immunity, inflammation, and tissue repair. Although epithelial cell-derived cytokines regulate ILC2 effector functions, the pathways that control the in vivo migration of ILC2s into inflamed tissues remain poorly understood. Here, we provide the first demonstration that expression of the prostaglandin D2 (PGD2) receptor CRTH2 (chemoattractant receptor-homologous molecule expressed on Th2 cells) regulates the in vivo accumulation of ILC2s in the lung. Although a significant proportion of ILC2s isolated from healthy human peripheral blood expressed CRTH2, a smaller proportion of ILC2s isolated from nondiseased human lung expressed CRTH2, suggesting that dynamic regulation of CRTH2 expression might be associated with the migration of ILC2s into tissues. Consistent with this, murine ILC2s expressed CRTH2, migrated toward PGD2 in vitro, and accumulated in the lung in response to PGD2 in vivo. Furthermore, mice deficient in CRTH2 exhibited reduced ILC2 responses and inflammation in a murine model of helminth-induced pulmonary type 2 inflammation. Critically, adoptive transfer of CRTH2-sufficient ILC2s restored pulmonary inflammation in CRTH2-deficient mice. Together, these data identify a role for the PGD2-CRTH2 pathway in regulating the in vivo accumulation of ILC2s and the development of type 2 inflammation in the lung.

[Testicular localization as the first manifestation of acute myeloblastic leukemia. Description of a case]. / Localizzazione testicolare come prima manifestazione di leucemia acuta mieloblastica. Descrizione di un caso.

Extramedullary masses as features of acute monoblastic leukemia are rare. Acute monoblastic leukemia is an uncommon type of nonlymphocytic leukemia that generally first manifest with signs and of bone marrow failure, articular and/or neurological symptoms. This study describes one patient with acute monoblastic leukemia in whom the initial manifestation of the disorder was related to testicular mass.

[Secondary varicocele as a clinical manifestation of primitive retroperitoneal tumor]. / Varicocele secondario come unica manifestazione clinica di tumore retroperitoneale primitivo.

The retroperitoneal primitive tumors are rare and the majority are malignant. Frequently the preoperative diagnosis is difficult because they show many clinical symptoms depending on the organs involved. A case of primitive retroperitoneal tumor with left varicocele syndrome as a first clinical manifestation is described.

First data on the distribution and ecology of Vibrio spp. of the Straits of Magellan (South America).

During the austral summer of 1991 a study was carried out on the presence and distribution of the genus Vibrio in the Straits of Magellan. Vibrios strains were isolated using membrane filters and Marine Agar 2216 in anaerobiosis. Variations of the populations of total heterotrophic bacteria and vibrios were observed both on the surface and along the column of water. All vibrios are psychrotrophic and were grouped in 4 cluster among which cluster 1, identified as presumed V. anguillarum, seems the most important including 73% of strains. A certain habitat segregation of clusters was noted. Cluster 4 was found only in a deep and permanently colder water mass. The relations between 20 environmental parameters and the bacterial population were also studied. Significant positive correlations were observed between the vibrios population and various fractions of suspended particulate matter.

Bacterial pollution of Messina coastal waters: a one year study.

A year's monitoring of faecal pollution of marine coastal waters surrounding Messina was carried out in 1996/97. The distribution of faecal coliforms was evaluated in 15 stations located along the Sicilian coastline, sampled monthly in coincidence of the two opposing current phases ("montante" and "scendente" currents) which characterise the Straits of Messina. The data obtained provided a complete picture of hygienic-sanitary conditions of the area and highlighted the presence of heavily polluted sites in correspondence with river outflows. Higher bacterial counts were associated with lower salinity values and higher ammonia concentrations; over an annual study, they occurred during the coldest months, showing the negative impact of continental water inputs on the bacteriological quality of coastal waters.

[Metastatic linitis plastica of the bladder]. / Linite plastica vescicale metastatica.

The adenocarcinoma is a rare istologic type of bladder cancer. A particular subset, the "signet ring cell carcinoma", can have the clinical expression of bladder linitis plastica. In the metastatic form this evenience is very rare and only two cases are reported in the literature. The Authors describe third case.

[Sex-dynamic characterization of patients subjected to radical pelvic cavity surgery]. / La caratterizzazione sessodinamica nei pazienti sottoposti a chirurgia radicale dello scavo pelvico.

A retrospective study was carried out using sexual case histories, hormonal profiles, vasoactive drugs test, dynamic cavernosometry and cavernosography on 46 patients who underwent radical cystectomy (26 patients: 57%) and radical prostatectomy (20 patients: 43%) for neoplasia, conducted by means of traditional surgical non-nerve sparing methods, in order to characterize the type of damage affecting the erectile function as a result of surgery. The study was carried out at a minimum of 2 months, a maximum of 9 years and 5 months, an average of 2 years and 6 months, after surgery. One patient (2%) reported intact erectile function and withdrew from the study. 37 patients (80%) showed neurological damage, while the remaining 8 (18%) had prevalently vascular damage.

[Isolation of V. cholerae no-01 and V. metschnikovii from sewage of the city of Mar del Plata]. / Aislamiento de V. cholerae no-01 y V. metschnikovii en efluentes cloacales de la ciudad de Mar del Plata.

A total of 42 vibrio strains isolated from sewage, together with 7 culture collection strains were studied by numerical taxonomy. A simplified clustering is shown in Fig. 1. Two important phena were determined. Phenon 1, identified as V. cholerae non-01, formed at 0.90% S, contains 32 sewage strains and 2 reference strains. Phenon 2, identified as V. metschnikovii, formed at 0.85% S, contains 5 sewage strains. No typical marine vibrio species were isolated. The phenotypic characteristics of 2 phena are shown in Table 2. All V. cholerae non-01 were haemolytic, proteolytic and lipolytic. They also showed some phenotypic characteristics (degradation of esculin, 81.2%; utilization of: D-glucuronate, 88.2%; cellobiose, 47.0% and lactose, 47.0%), and growth in NaCl 6%, 91.1%. According to literature references, the survival of V. cholerae non-01 in Mar del Plata marine environment is possible.

[Isolation and quantification of Salmonella in the waters of the Río de la Plata destined for recreation]. / Aislamiento y cuantificación de Salmonella en aguas del Río de la Plata destinadas a recreación.

Thirty three samples from recreational waters from different areas of Río de la Plata were tested for Salmonella (Fig. 1). A 200 ml sample filtered through a diatom bed as well as 2 and 20 ml samples without filtering were studied using a simple and economical technique. The isolation probability was directly dependent on the water volume analyzed. Best results were obtained when tetrathionate broth with novobiocin and Salmonella shigella agar were used (Table 3). Salmonella was isolated from 36% of the samples studied and 12 serotypes were identified (Table 5). The relation between the NPM of each bacteriological indicator used and the NPM of Salmonella are shown in Table 4. Correlation values were calculated by relating mean point logarithms of the classes in Table 4 with the isolation percentages of Salmonella for each class. These values were low, except for fecal Streptococcus (R2 = 0.94). The high density of fecal coliforms found in Bagliardi and Pescadores Beaches (rarely less than 10.000 bacteria/100 ml) and the incidence of Salmonella, lead us to conclude that these waters are unsafe for bathing and swimming, specially for children, according with the international recommendation on the quality of recreational waters. Waters with the characteristics described here should be periodically analyzed for Salmonella concentration. The lack of correlation values between the MPN of total and fecal coliforms and the MPN of Salmonella, also supports this advise.

A coarse-grained MARTINI model of polyethylene glycol and of polyoxyethylene alkyl ether surfactants.

Nonionic surfactants are used for the isolation and purification of membrane proteins, as well as for the study of fundamental aspects of protein diffusion in membranes. Here we present a new coarse-grained model of polyethylene glycol (PEG) and of the family of polyoxyethylene alkyl ether (C(i)E(j)) surfactants. The model is compatible with the MARTINI coarse-grained force-field for lipids and proteins. We validate the model by comparing molecular dynamics simulations with experimental data. In particular, we show that the model reproduces the phase behavior of water-surfactant mixtures as a function of water concentration. We also simulate the self-assembly of two ternary mixtures that have been used for the experimental measure of protein diffusion coefficients. The first includes a cosurfactant that affects the curvature of the surfactant bilayers; the second is a mixture of C(i)E(j) surfactants, alkanes and water. In both cases, the results of self-assembly simulations are in agreement with experimental observations and pave the way to the use of the surfactant model in combination with MARTINI peptides and proteins.

Assessment of the ecological status of transitional waters in Sicily (Italy): first characterisation and classification according to a multiparametric approach.

A 1-year cycle of observations was performed in four Sicilian transitional water systems (Oliveri-Tindari, Cape Peloro, Vendicari and Marsala) to characterise their ecological status. A panel of variables among which trophic and microbial (enzyme activities, abundance of hetetrophic bacteria and of bacterial pollution indicators) parameters, were selected. Particulate organic carbon (POC) and nitrogen (PON) and chlorophyll-a (Chl-a) contents defined the trophic state, while microbial hydrolysis rates and abundance gave insights on microbial community efficiency in organic matter transformation and on allochthonous inputs. To classify the trophic state of examined waters, the synthetic trophic state index (TRIX) was calculated. Microbial hydrolysis rates correlated positively with POC and Chl-a, which increased along the eutrophication gradient. The significant relationships among TRIX, trophic and microbial parameters suggested the use of leucine aminopeptidase, alkaline phosphatase and POC as suitable parameters to implement the Water Framework Directive when assessing the ecological status of transitional water systems.

Microbial community dynamics during assays of harbour oil spill bioremediation: a microscale simulation study.

AIMS: Microcosm experiments simulating an oil spill event were performed to evaluate the response of the natural microbial community structure of Messina harbour seawater following the accidental load of petroleum. METHODS AND RESULTS: An experimental harbour seawater microcosm, supplemented with nutrients and crude oil, was monitored above 15 days in comparison with unpolluted ones (control microcosms). Bacterial cells were counted with a Live/Dead BacLight viability kit; leucine aminopeptidase, beta-glucosidase, alkaline phosphatase, lipase and esterase enzymes were measured using fluorogenic substrates. The microbial community dynamic was monitored by isolation of total RNA, RT-PCR amplification of 16S rRNA, cloning and sequencing. Oil addition stimulated an increase of the total bacterial abundance, leucine aminopeptidase and phosphatase activity rates, as well as a change in the community structure. This suggested a prompt response of micro-organisms to the load of petroleum hydrocarbons. CONCLUSIONS: The present study on the viability, specific composition and metabolic characteristics of the microbial community allows a more precise assessment of oil pollution. Both structural and functional parameters offer interesting perspectives as indicators to monitor changes caused by petroleum hydrocarbons. SIGNIFICANCE AND IMPACT OF THE STUDY: A better knowledge of microbial structural successions at oil-polluted sites is essential for environmental bioremediation. Data obtained in microcosm studies improve our understanding of natural processes occurring during oil spills.

Structural investigation of syringomycin-E using molecular dynamics simulation and NMR.

Syringomycin-E (SR-E) is a cyclic lipodepsinonapeptide produced by certain strains of the bacterium Pseudomonas syringae pv. syringae. It shows inhibitory effects against many fungal species, including human pathogens. Its primary biological target is the plasma membrane, where it forms channels comprised of at least six SR-E molecules. The high-resolution structure of SR-E and the structure of the channels are currently not known. In this paper, we investigate in atomic detail the molecular features of SR-E in water by NMR and in water and octane by molecular dynamics simulation (MD). We built a model of the peptide and examined its structure in water and octane in 200 ns MD simulations both with and without distance restraints derived from NMR NOE data. The resulting trajectories show good agreement with the measured NOEs and circular dichroism data from the literature and provide atomistic models of SR-E that are an important step toward a better understanding of the antifungal and antibacterial activity of this peptide.

[Ureteritis]. / Le ureteriti.

We relate our experience about ureteritis, especially non specific ureteritis. The traumatic, radiation ureteritis will be discussed in others chapters. Most cases of ureteritis are infective, and may be due to any of the organism normally found in urinary tract infections, particularly Escherichia Coli, staphylococci, streptococci, enterococci, proteus and pyocyaneus. It is really primary, but it usually ascending from an associated cystitis, descending from pyelonephritis, or due to direct spread from and adjacent inflammatory lesion such as appendicitis or salpingitis. The infection may also reach the ureter by lymphatic spread, particularly from the prostate and seminal vesicles. Any associated abnormalities of the ureter, such as stricture, megaloureter, ureterocele, and so on, will naturally predispose to infective ureteritis. As ureteritis is rarely primary, the first step in treatment must be toward the elucidation and cure of any underlying lesion. Thus calculi, cystitis, pyelitis, and so on, will need appropriate therapy, and this in itself will considerably improve or cure the ureteritis, and specially in the more acute cases. In the chronic cases with stricture formation, dilation or even excision of the stenosed portion may be required. For the treatment of the strictures we want emphasize the role of the ureteral stenting thinking its use is necessary to preserve the renal function.