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Plant Physiol ; 167(2): 394-410, 2015 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-25540331

RESUMEN

The intercellular movement of plant viruses requires both viral and host proteins. Previous studies have demonstrated that the frame-shift protein P3N-PIPO (for the protein encoded by the open reading frame [ORF] containing 5'-terminus of P3 and a +2 frame-shift ORF called Pretty Interesting Potyviridae ORF and embedded in the P3) and CYLINDRICAL INCLUSION (CI) proteins were required for potyvirus cell-to-cell movement. Here, we provide genetic evidence showing that a Tobacco vein banding mosaic virus (TVBMV; genus Potyvirus) mutant carrying a truncated PIPO domain of 58 amino acid residues could move between cells and induce systemic infection in Nicotiana benthamiana plants; mutants carrying a PIPO domain of seven, 20, or 43 amino acid residues failed to move between cells and cause systemic infection in this host plant. Interestingly, the movement-defective mutants produced progeny that eliminated the previously introduced stop codons and thus restored their systemic movement ability. We also present evidence showing that a developmentally regulated plasma membrane protein of N. benthamiana (referred to as NbDREPP) interacted with both P3N-PIPO and CI of the movement-competent TVBMV. The knockdown of NbDREPP gene expression in N. benthamiana impeded the cell-to-cell movement of TVBMV. NbDREPP was shown to colocalize with TVBMV P3N-PIPO and CI at plasmodesmata (PD) and traffic to PD via the early secretory pathway and the actomyosin motility system. We also show that myosin XI-2 is specially required for transporting NbDREPP to PD. In conclusion, NbDREPP is a key host protein within the early secretory pathway and the actomyosin motility system that interacts with two movement proteins and influences virus movement.


Asunto(s)
Actomiosina/metabolismo , Nicotiana/metabolismo , Proteínas de Plantas/metabolismo , Plasmodesmos/metabolismo , Vías Secretoras , Citoesqueleto de Actina/efectos de los fármacos , Citoesqueleto de Actina/metabolismo , Secuencia de Aminoácidos , Brefeldino A/farmacología , Compuestos Bicíclicos Heterocíclicos con Puentes/farmacología , Silenciador del Gen/efectos de los fármacos , Proteínas Fluorescentes Verdes/metabolismo , Modelos Biológicos , Datos de Secuencia Molecular , Movimiento/efectos de los fármacos , Mutación , Miosinas/metabolismo , Enfermedades de las Plantas/virología , Plasmodesmos/efectos de los fármacos , Potyvirus/patogenicidad , Unión Proteica/efectos de los fármacos , Estructura Terciaria de Proteína , Transporte de Proteínas/efectos de los fármacos , Proteínas Recombinantes de Fusión/metabolismo , Saccharomyces cerevisiae/metabolismo , Vías Secretoras/efectos de los fármacos , Fracciones Subcelulares/efectos de los fármacos , Fracciones Subcelulares/metabolismo , Tiazolidinas/farmacología , Nicotiana/efectos de los fármacos , Proteínas Virales/química , Proteínas Virales/metabolismo
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