RESUMEN
Boron-neutron capture therapy (BNCT) is an attractive technique for cancer treatment. As such, α, α-cycloalkyl amino acids containing thiododecaborate ([B12H11](2-)-S-) units were designed and synthesized as novel boron delivery agents for BNCT. In the present study, new thiododecaborate α, α-cycloalkyl amino acids were synthesized, and biological evaluation of the boron compounds as boron carrier for BNCT was carried out.
Asunto(s)
Compuestos de Boro/síntesis química , Compuestos de Boro/farmacología , Terapia por Captura de Neutrón de Boro/instrumentación , Neoplasias Encefálicas/radioterapia , Radiofármacos/síntesis química , Radiofármacos/farmacología , Aminoácidos/química , Neoplasias Encefálicas/fisiopatología , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , HumanosRESUMEN
Polyclonal antibodies consist of a mixture of antibodies produced by multiple B-cell clones that have differentiated into antibody-producing plasma cells in response to an immunogen. Polyclonal antibodies raised against an antigen recognize multiple epitopes on a target molecule, which results in a signal amplification in indirect immunoassays including immune-electron microscopy. In this chapter, we present a basic procedure to generate polyclonal antibodies in rabbits.
Asunto(s)
Anticuerpos/aislamiento & purificación , Formación de Anticuerpos , Antígenos/química , Epítopos/química , Sulfato de Amonio/química , Animales , Anticuerpos/sangre , Antígenos/inmunología , Linfocitos B/citología , Linfocitos B/inmunología , Diferenciación Celular , Ensayo de Inmunoadsorción Enzimática , Epítopos/inmunología , Adyuvante de Freund/administración & dosificación , Células Plasmáticas/citología , Células Plasmáticas/inmunología , ConejosRESUMEN
To develop a boron carrier for practical purposes, new boron-containing amino acids with an undecahydro-closo-dodecaboranylthio ([(10)B(12)H(11)S](2-)-) unit in the side chain of the α-amino acid have already been designed and synthesized. In the present paper, cytotoxicity, the incorporation amounts into tumor cells, and the tumor cell killing effects of these compounds were elucidated to evaluate their usefulness as boron carriers. Furthermore, the microdistribution of the amino acids in tumor cells was established.
Asunto(s)
Aminoácidos/farmacología , Boranos/farmacología , Aminoácidos/química , Aminoácidos/farmacocinética , Animales , Boranos/química , Boranos/farmacocinética , Terapia por Captura de Neutrón de Boro , Línea Celular Tumoral , Humanos , Ratones , Ratas , EstereoisomerismoRESUMEN
Polyclonal antibodies are derived from multiple B-cell clones that have differentiated into antibody-producing plasma cells in response to an immunogen. Polyclonal antibodies raised against a single molecular species of antigen recognize multiple epitopes on a target molecule resulting in signal amplification in indirect immunoassays, including immuno-electron microscopy. In this chapter, we present a basic procedure to generate polyclonal antibodies in rabbits.
Asunto(s)
Anticuerpos/inmunología , Anticuerpos/aislamiento & purificación , Sueros Inmunes/biosíntesis , Sueros Inmunes/inmunología , Animales , Femenino , ConejosRESUMEN
Monoclonal antibodies (mAb's) have been used extensively in biochemical and biomedical studies, including immunoelectron microscopy. Production of mAb's consists of four steps: immunizing the animal usually a mouse, obtaining immune cells from the spleen of the immunized mouse, fusing the spleen cells with myeloma cells to obtain hybridomas, and selecting the hybridoma cell line producing the desired mAb. In this chapter, we present a general method for monoclonal antibody production.