Impact of a Rapid Results Initiative Approach on Improving Male Partner Involvement in Prevention of Mother to Child Transmission of HIV in Western Kenya.

A rapid results initiative (RRI) aimed at increasing male involvement in prevention of mother-to-child transmission (PMTCT) and service uptake among pregnant women at 116 antenatal clinics in Western Kenya was compared at baseline, during the RRI, and 3-months post-RRI. Male involvement increased from 7.4 to 54.2% during RRI (risk difference [RD] 0.47, CI 0.45-0.48) then 43.4% post-RRI (RD 0.36, CI 0.35-0.37). Among HIV-infected women, facility delivery increased from 40.0 to 49.9% (RD 0.10, 95% CI 0.06-0.13) and 65.0% post-RRI (RD 0.25, 95% CI 0.22-0.28). HIV-infected pregnant women linkage to HIV care increased from 58.6 to 85.9% (RD 0.27, CI 0.24-0.30) and 97.3% post-RRI (RD 0.39, CI 0.36-0.41). Time to ART initiation reduced from 29 days (interquartile range [IQR] 6-56) to 14 days (IQR 0-28) to 7 days (IQR 0-20). A male-centered RRI can significantly increase men's engagement in antenatal care leading to improved partner utilization of PMTCT and antenatal services.

Reducing spatial variability of soybean response to rhizobia inoculants in farms of variable soil fertility in Siaya County of western Kenya.

Soybean grain yields in sub-Saharan Africa have remained at approximately 50% below those attained in South America despite numerous efforts. A study was conducted in Siaya County (western Kenya) involving 107 farms with soils of different fertility status. The main objective was to test combinations of two inoculants (Legumefix and Biofix) and nutrient sources (Minjingu and Sympal) to raise soybean grain yields. Inoculation used Legumefix or Biofix with and without Minjingu or Sympal in a factorial design. There was soil acidity and a widespread deficiency of potassium, nitrogen, and phosphorus. Inoculation and nutrient source led to increases in nodulation and nodule occupancy. For grain yields the response varied from nil in some sites to high increases in others. Highest grain yields (3000-4000 kg ha-1) were obtained with Legumefix + Sympal (12% of the farmers testing it). The formulation of the nutrient source was important to meet other nutrient deficiencies in most of the soils. Farmers using Legumefix + Sympal require yield increases of 35% for profitability (Value cost ratio of 3) while farmers using Minjingu + inoculant require a yield increase of at least 68%. Inoculants used alone were most profitable but this is advisable only when farmers are too resource constrained to afford fertilizer. For sustainable yields Legumefix + Sympal or Biofix + Sympal were recommended.

Genetic structure and relationships within and between cultivated and wild sorghum (Sorghum bicolor (L.) Moench) in Kenya as revealed by microsatellite markers.

Understanding the extent and partitioning of diversity within and among crop landraces and their wild/weedy relatives constitutes the first step in conserving and unlocking their genetic potential. This study aimed to characterize the genetic structure and relationships within and between cultivated and wild sorghum at country scale in Kenya, and to elucidate some of the underlying evolutionary mechanisms. We analyzed at total of 439 individuals comprising 329 cultivated and 110 wild sorghums using 24 microsatellite markers. We observed a total of 295 alleles across all loci and individuals, with 257 different alleles being detected in the cultivated sorghum gene pool and 238 alleles in the wild sorghum gene pool. We found that the wild sorghum gene pool harbored significantly more genetic diversity than its domesticated counterpart, a reflection that domestication of sorghum was accompanied by a genetic bottleneck. Overall, our study found close genetic proximity between cultivated sorghum and its wild progenitor, with the extent of crop-wild divergence varying among cultivation regions. The observed genetic proximity may have arisen primarily due to historical and/or contemporary gene flow between the two congeners, with differences in farmers' practices explaining inter-regional gene flow differences. This suggests that deployment of transgenic sorghum in Kenya may lead to escape of transgenes into wild-weedy sorghum relatives. In both cultivated and wild sorghum, genetic diversity was found to be structured more along geographical level than agro-climatic level. This indicated that gene flow and genetic drift contributed to shaping the contemporary genetic structure in the two congeners. Spatial autocorrelation analysis revealed a strong spatial genetic structure in both cultivated and wild sorghums at the country scale, which could be explained by medium- to long-distance seed movement.