Study of the optimum level of electrode placement for the evaluation of absolute lung resistivity with the Mk3.5 EIT system.

Inter-subject variability has caused the majority of previous electrical impedance tomography (EIT) techniques to focus on the derivation of relative or difference measures of in vivo tissue resistivity. Implicit in these techniques is the requirement for a reference or previously defined data set. This study assesses the accuracy and optimum electrode placement strategy for a recently developed method which estimates an absolute value of organ resistivity without recourse to a reference data set. Since this measurement of tissue resistivity is absolute, in Ohm metres, it should be possible to use EIT measurements for the objective diagnosis of lung diseases such as pulmonary oedema and emphysema. However, the stability and reproducibility of the method have not yet been investigated fully. To investigate these problems, this study used a Sheffield Mk3.5 system which was configured to operate with eight measurement electrodes. As a result of this study, the absolute resistivity measurement was found to be insensitive to the electrode level between 4 and 5 cm above the xiphoid process. The level of the electrode plane was varied between 2 cm and 7 cm above the xiphoid process. Absolute lung resistivity in 18 normal subjects (age 22.6 +/- 4.9, height 169.1 +/- 5.7 cm, weight 60.6 +/- 4.5 kg, body mass index 21.2 +/- 1.6: mean +/- standard deviation) was measured during both normal and deep breathing for 1 min. Three sets of measurements were made over a period of several days on each of nine of the normal male subjects. No significant differences in absolute lung resistivity were found, either during normal tidal breathing between the electrode levels of 4 and 5 cm (9.3 +/- 2.4 Omega m, 9.6 +/- 1.9 Omega m at 4 and 5 cm, respectively: mean +/- standard deviation) or during deep breathing between the electrode levels of 4 and 5 cm (10.9 +/- 2.9 Omega m and 11.1 +/- 2.3 Omega m, respectively: mean +/- standard deviation). However, the differences in absolute lung resistivity between normal and deep tidal breathing at the same electrode level are significant. No significant difference was found in the coefficient of variation between the electrode levels of 4 and 5 cm (9.5 +/- 3.6%, 8.5 +/- 3.2% at 4 and 5 cm, respectively: mean +/- standard deviation in individual subjects). Therefore, the electrode levels of 4 and 5 cm above the xiphoid process showed reasonable reliability in the measurement of absolute lung resistivity both among individuals and over time.

Detection of emboli in vessels using electrical impedance measurements--phantom and electrodes.

A phantom was constructed to simulate the electrical properties of the neck. A range of possible electrode configurations was then examined in order to improve the sensitivity of the impedance measurement method for the in vivo detection of air emboli. The neck phantom consisted of simulated skin, fat and muscle layers made of agar and a conductive rubber tube mimicking the common carotid artery. The ring-shaped electrodes with a guard electrode showed the highest sensitivity to emboli at short distances.

Facilitation of motor evoked potentials in the anterior tibial muscle by repetitive subthreshold electrical stimulation.

Subthreshold electrical stimulation with an intensity less than the threshold for evoking M-waves is applied repetitively to the common peroneal nerve via surface electrodes. The stimulation intensity is varied by adjusting the pulse width from 0 to 240 micros, while the pulse interval (40 ms) and current amplitude are kept constant. Single magnetic stimuli are applied to the motor cortex using a circular coil. Motor evoked potentials are recorded from the anterior tibial muscle in six normal subjects for various subthreshold stimulation intensities. Signal processing (filtering in the time and frequency domains) removes the artifact caused by the subthreshold electrical stimulation from the motor evoked potential. Statistically significant motor evoked potential facilitation (p < 0.05) is observed for pulse widths ranging from 72 to 240 micros in all the tested subjects. A pulse width corresponding to 90% of the electrical threshold facilitated the motor evoked potential in five of the six subjects.

Accuracy of an optically isolated tetra-polar impedance measurement system.

Accurate electrical transfer impedance measurement at the high frequencies (> 1 MHz) required to characterise blood and intracellular structures is very difficult, owing to stray capacitances between lead wires. To solve this problem, an optically isolated measurement system has been developed using a phase-locked-loop technique for synchronisation between current injection (drive) and voltage measurement (receive) circuits. The synchronisation error between drive and receive circuits was less than 1 ns. The accuracy and reproducibility of the developed system was examined using a tissue equivalent Cole model consisting of two resistors and one capacitor. The absolute value Z and phase shift theta in impedance of the Cole model was measured at 1.25 MHz by both an LCR meter and the isolated measurement system. The difference between the values measured by the isolated measurement system and those measured by the LCR meter was less than 0.27omega (2.9%) in Z and 0.79 degree in theta. The standard deviation was less than 0.09 omega in Z and 0.60 degree in theta.

Estimation of the size of air emboli detectable by electrical impedance measurement.

Non-invasive detection of air emboli in blood is investigated in vitro using a tetrapolar electrical impedance measurement. A cubic tank with a linear array of four electrodes, spaced approximately 1 cm apart down one side, was filled with 0.2 Sm(-1) saline. Bubbles were generated by carbon dioxide gas. Electrical transfer impedance was measured every 8.2 ms at 1.25 MHz. The movement of bubbles was recorded by a video camera, and their sizes and depths from the middle of the array were measured using captured video images. Changes in transfer impedance caused by passage of bubbles were clearly observed and almost identical with those calculated theoretically. Using lead field theory and experimental results, the fundamental limit on the detectable size of bubbles was estimated at the carotid artery, the great saphenous vein and the cephalic vein. The theoretical results showed that a 0.5 mm diameter bubble is detectable at a depth of 5.3 mm, similar to the depth of the great saphenous and the cephalic veins, and a 2.3 mm diameter bubble is detectable at a depth of 21 mm, similar to the depth of the common carotid artery.