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1.
Glob Chang Biol ; 28(9): 2846-2874, 2022 05.
Artículo en Inglés | MEDLINE | ID: mdl-35098619

RESUMEN

The two most urgent and interlinked environmental challenges humanity faces are climate change and biodiversity loss. We are entering a pivotal decade for both the international biodiversity and climate change agendas with the sharpening of ambitious strategies and targets by the Convention on Biological Diversity and the United Nations Framework Convention on Climate Change. Within their respective Conventions, the biodiversity and climate interlinked challenges have largely been addressed separately. There is evidence that conservation actions that halt, slow or reverse biodiversity loss can simultaneously slow anthropogenic mediated climate change significantly. This review highlights conservation actions which have the largest potential for mitigation of climate change. We note that conservation actions have mainly synergistic benefits and few antagonistic trade-offs with climate change mitigation. Specifically, we identify direct co-benefits in 14 out of the 21 action targets of the draft post-2020 global biodiversity framework of the Convention on Biological Diversity, notwithstanding the many indirect links that can also support both biodiversity conservation and climate change mitigation. These relationships are context and scale-dependent; therefore, we showcase examples of local biodiversity conservation actions that can be incentivized, guided and prioritized by global objectives and targets. The close interlinkages between biodiversity, climate change mitigation, other nature's contributions to people and good quality of life are seldom as integrated as they should be in management and policy. This review aims to re-emphasize the vital relationships between biodiversity conservation actions and climate change mitigation in a timely manner, in support to major Conferences of Parties that are about to negotiate strategic frameworks and international goals for the decades to come.


Asunto(s)
Conservación de los Recursos Naturales , Calidad de Vida , Biodiversidad , Cambio Climático , Ecosistema , Humanos
2.
Nature ; 540(7632): 220-229, 2016 12 08.
Artículo en Inglés | MEDLINE | ID: mdl-27894123

RESUMEN

Wild and managed pollinators provide a wide range of benefits to society in terms of contributions to food security, farmer and beekeeper livelihoods, social and cultural values, as well as the maintenance of wider biodiversity and ecosystem stability. Pollinators face numerous threats, including changes in land-use and management intensity, climate change, pesticides and genetically modified crops, pollinator management and pathogens, and invasive alien species. There are well-documented declines in some wild and managed pollinators in several regions of the world. However, many effective policy and management responses can be implemented to safeguard pollinators and sustain pollination services.


Asunto(s)
Conservación de los Recursos Naturales/tendencias , Producción de Cultivos , Política Ambiental/tendencias , Insectos/fisiología , Polinización , Vertebrados/fisiología , Animales , Abejas/fisiología , Mariposas Diurnas/fisiología , Cambio Climático , Producción de Cultivos/economía , Productos Agrícolas/genética , Productos Agrícolas/fisiología , Ecosistema , Humanos , Especies Introducidas , Plaguicidas/efectos adversos , Plaguicidas/toxicidad , Plantas Modificadas Genéticamente/efectos de los fármacos , Plantas Modificadas Genéticamente/genética , Dinámica Poblacional
3.
Int J Syst Evol Microbiol ; 70(9): 4903-4907, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-32821034

RESUMEN

A Gram-stain-negative, facultative anaerobic, motile, short rods and yellow-pigmented bacterium, designated strain THG-DN7.12T, was isolated from water collected at Jungwon waterfall on Yongmun mountain, Republic of Korea. According to 16S rRNA gene sequence comparisons, strain THG-DN7.12T was found to be most closely related to Aquitalea denitrificans 5YN1-3T (98.9 % sequence similarity), Aquitalea magnusonii TRO-001DR8T (98.7 %) and Aquitalea pelogenes P1297T (98.0 %). The DNA-DNA relatedness between strain THG-DN7.12T and its phylogenetically closest neighbours was below 70.0 %. The strain's DNA G+C content was 59.7 mol%. The major polar lipid was found to be phosphatidylethanolamine. Summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0 were identified as the major fatty acids. Ubiquinone Q-8 was detected as the only respiratory quinone. These data supported the affiliation of strain THG-DN7.12T to the genus Aquitalea. Strain THG-DN7.12T was distinguished from related Aquitalea species by physiological and biochemical tests. Therefore, the novel isolate represents a novel species, for which the name Aquitalea aquatilis sp. nov. is proposed, with THG-DN7.12T as the type strain (=KACC 18847T=CCTCC AB 2016185T).


Asunto(s)
Betaproteobacteria/clasificación , Agua Dulce , Filogenia , Microbiología del Agua , Técnicas de Tipificación Bacteriana , Composición de Base , Betaproteobacteria/aislamiento & purificación , ADN Bacteriano/genética , Ácidos Grasos/química , Hibridación de Ácido Nucleico , Fosfatidiletanolaminas/química , Pigmentación , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Ubiquinona/química
4.
Biosci Biotechnol Biochem ; 84(11): 2327-2336, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-32698696

RESUMEN

According to the previously described anti-photoaging effect of the enzyme-processed Panax ginseng extract and Gastrodia elata extract, we hypothesized that the combination of the two extracts would have superior effect to protect human skin from UVB radiation. Besides, the mixture of active components isolated from herbal extracts, ginsenoside F2, and α-gastrodin was investigated on the photo-protective capability. The expression of aging-related markers including matrix metalloproteinase-1 (MMP-1), interleukin-6 (IL-6), and procollagen type 1 was evaluated using ELISA kits. It was reported that the herbal extract at a Panax ginseng extract to Gastrodia elata extract ratio of 1:10 (w/w) and the compound mixture with equal proportion of ginsenoside F2 and α-gastrodin exhibited significant inhibition of MMP-1 and IL-6 production, and marked upregulation of procollagen type 1 formation. Thus, the combination of either the enzyme-processed herbal extracts or their active components would enhance the properties of prevention and treatment of UVB-induced skin damage.


Asunto(s)
Colágeno Tipo I/metabolismo , Fibroblastos/efectos de los fármacos , Gastrodia/química , Interleucina-6/metabolismo , Metaloproteinasa 1 de la Matriz/metabolismo , Panax/química , Extractos Vegetales/farmacología , Supervivencia Celular/efectos de los fármacos , Fibroblastos/citología , Fibroblastos/metabolismo , Fibroblastos/efectos de la radiación , Humanos , Protectores contra Radiación/farmacología , Piel/citología , Rayos Ultravioleta/efectos adversos
5.
J Cell Biochem ; 120(1): 601-612, 2019 01.
Artículo en Inglés | MEDLINE | ID: mdl-30195253

RESUMEN

BACKGROUND: Excessive ultraviolet radiation usually causes skin photoaging, inflammation, and even photocarcinogenesis. UV radiation-generated reactive oxygen species (ROS) are a major contributing factor to photodamage. The flowers of Helianthus annuus L. have been reported to possess strong anti-inflammatory and antioxidant activity. However, there are few reports on the use of H. annuus L. to relieve UVB-induced photoaging. PURPOSE: In this study, we evaluated the protective effect of a 50% ethanol extract of H. annuus L. flower (HAF) against UVB-induced photodamage using normal human dermal fibroblasts. METHODS: The secretion of ROS, interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMPs), procollagen type I, and transforming growth factor-ß1 (TGF-ß1) was measured with kits. The messenger RNA levels of COX-2, iNOS, and TGF-α were measured by RT-PCR. The AP-1, MAPK, NFAT, and Nrf2 pathways were investigated by Western blot analysis. RESULTS: HAF extract significantly blocked UVB-induced ROS and MMP (MMP-1 and MMP-3) production and procollagen type I reduction. Further study demonstrated that the photoaging inhibitory actions were related to promotion of Nrf2 nuclear translocation, upregulation of TGF-ß1 level, and downregulation of AP-1 and MAPK phosphorylation. Importantly, HAF effectively inhibited UVB-induced VEGF and inflammatory cytokines such as IL-6, COX-2, iNOS, and TNF-α secretion, which might be involved in the regulation of the NFAT signaling pathway. CONCLUSION: Our results indicate that HAF is a useful botanical source protecting against UVB-mediated skin photodamage.


Asunto(s)
Antioxidantes/farmacología , Fibroblastos/metabolismo , Helianthus/química , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Factor 2 Relacionado con NF-E2/metabolismo , Factores de Transcripción NFATC/metabolismo , Extractos Vegetales/farmacología , Factor de Transcripción AP-1/metabolismo , Rayos Ultravioleta/efectos adversos , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/efectos de la radiación , Colágeno Tipo I/metabolismo , Citocinas/metabolismo , Etanol/química , Flores/química , Humanos , Metaloproteinasa 1 de la Matriz/metabolismo , Metaloproteinasa 3 de la Matriz/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Transducción de Señal/efectos de los fármacos , Transducción de Señal/efectos de la radiación , Piel/citología , Envejecimiento de la Piel/fisiología
6.
Arch Biochem Biophys ; 662: 190-200, 2019 02 15.
Artículo en Inglés | MEDLINE | ID: mdl-30571966

RESUMEN

Exposure of skin to UVB radiation is associated with skin thickening, erythema, deep wrinkles, and marked losses of elasticity, resilience, and hydration. To find effective anti-aging materials, scientists have studied not only natural nutritional sources, but also biotransformed metabolites. Although Hibiscus syriacus L., the national flower of Korea has been used extensively throughout history, it has not yet been examined for possible anti-aging effects. In this study, skin anti-aging effects of H. syriacus L. water extract (HSL) and enzyme-treated H. syriacus L. extract (ETH) were investigated in normal human dermal fibroblasts (NHDFs) in vitro and in hairless mice in vivo. In UVB-irradiated NHDFs, higher level of type I procollagen and lower levels of matrix metalloproteinase-1 (MMP-1), mitogen-activated protein kinases (MAPKs), and activator protein-1 (AP-1) expression were identified after treatment with HSL and ETH. In photoaged hairless mice, skin thickening and the density of collagen fibers and filaggrin improved after oral administration of HSL and ETH. ETH 1% significantly inhibited melanin content, erythema index (EI), transepidermal water loss (TEWL), stratum corneum (SC) hydration, and wrinkle formation. Palmitic acid and linoleic acid were more abundant in ETH than in HSL. Taken together, both HSL and ETH protect skin from UVB-induced premature photoaging, and enzymatic biotransformed products like ETH have potential for use as valuable functional foods.


Asunto(s)
Enzimas/farmacología , Hibiscus/química , Extractos Vegetales/farmacología , Envejecimiento de la Piel/efectos de los fármacos , Piel/efectos de la radiación , Rayos Ultravioleta , Agua/análisis , Animales , Antioxidantes/farmacología , Células Cultivadas , Proteínas Filagrina , Alimentos Funcionales , Humanos , Masculino , Ratones , Ratones Pelados , Transducción de Señal/efectos de la radiación , Piel/citología
7.
Photochem Photobiol Sci ; 18(1): 260, 2019 01 01.
Artículo en Inglés | MEDLINE | ID: mdl-30484803

RESUMEN

Correction for 'Icariin and icaritin recover UVB-induced photoaging by stimulating Nrf2/ARE and reducing AP-1 and NF-κB signaling pathways: a comparative study on UVB-irradiated human keratinocytes' by Eunson Hwang et al., Photochem. Photobiol. Sci., 2018, 17, 1396-1408.

8.
Int J Mol Sci ; 21(1)2019 Dec 19.
Artículo en Inglés | MEDLINE | ID: mdl-31861704

RESUMEN

Human adipose-derived mesenchymal stem cells-conditioned medium (ADSC-CM) contains cytokines and growth factors that can facilitate the regeneration and repair of various tissues and organs. In the present study, the protective activity of ADSC-CM treatment was investigated in UVB-irradiated human keratinocyte cell line HaCaTs and normal human dermal fibroblasts (NHDFs). It was found that ADSC-CM can modulate the expression of the signaling molecules in the early UVB responsive signaling pathways, including mitogen activated protein kinases (MAPKs), activator protein 1 (AP-1), and nuclear factor kappa B (NF-κB). In addition, ADSC-CM treatment could upregulate antioxidant response element (ARE) such as phase II gene heme oxygenase-1 (HO-1) and increase the expression of collagen synthesis enhancer gene transforming growth factor-ß (TGF-ß). The expression of matrix metalloproteinase-1 (MMP-1) and procollagen type I synthesis inhibitors such as interleukin-6 (IL-6) was also found to be suppressed upon ADSC-CM treatment. Taken together, our study illustrates the anti-photoaging activities of ADSC-CM in cell-based models.


Asunto(s)
Tejido Adiposo/citología , Medios de Cultivo Condicionados/farmacología , Queratinocitos/citología , Células Madre Mesenquimatosas/citología , Envejecimiento de la Piel/efectos de los fármacos , Tejido Adiposo/metabolismo , Técnicas de Cultivo de Célula , Células Cultivadas , Citocinas/metabolismo , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Fibroblastos/efectos de la radiación , Regulación de la Expresión Génica/efectos de los fármacos , Regulación de la Expresión Génica/efectos de la radiación , Humanos , Queratinocitos/efectos de los fármacos , Queratinocitos/efectos de la radiación , Metaloproteinasa 1 de la Matriz/metabolismo , Células Madre Mesenquimatosas/metabolismo , Transducción de Señal/efectos de los fármacos , Transducción de Señal/efectos de la radiación , Rayos Ultravioleta/efectos adversos
9.
Photochem Photobiol Sci ; 17(10): 1396-1408, 2018 Oct 10.
Artículo en Inglés | MEDLINE | ID: mdl-30225503

RESUMEN

Icariin (ICA) and icaritin (ICT) exhibit many pharmacological functions including anti-osteoporosis, anti-cardiovascular, and anti-cancer activities; however, there are few comprehensive studies that track the detailed effects on UVB-induced photoaging. The recovery effects of ICA and ICT were investigated in UVB-irradiated human keratinocytes (HaCaTs). The results indicated that ICT and ICA showed strong radical scavenging activity, and the reactive oxygen species (ROS) scavenging activity of ICT was superior. UVB-induced matrix metalloproteinase-1 (MMP-1) expression was blocked by ICA via the inhibition of mitogen-activated protein kinase/activator protein 1 (MAPK/AP-1), which directly reduced extracellular matrix (ECM) degradation. ICT activated nuclear factor erythroid 2 related factor 2 (Nrf2) to improve the anti-oxidative stress capacity and suppress nuclear factor-κB (NF-κB) activation, decreasing vascular endothelial growth factor (VEGF) protein, and inflammatory cytokines induced ECM degrading enzyme secretion. Moreover, ICT was more advantageous to improve transforming growth factor beta 1 (TGF-ß1) and procollagen type I expression than ICA, promoting the synthesis of collagen. Therefore, ICA and ICT have potential to treat UVB-induced oxidative stress, inflammation and photoaging, and will be posited as a novel strategy to alleviate photodamage.


Asunto(s)
Senescencia Celular/efectos de los fármacos , Senescencia Celular/efectos de la radiación , Flavonoides/farmacología , Queratinocitos/efectos de los fármacos , Queratinocitos/efectos de la radiación , Protectores contra Radiación/farmacología , Antineoplásicos/farmacología , Elementos de Respuesta Antioxidante/efectos de los fármacos , Elementos de Respuesta Antioxidante/efectos de la radiación , Línea Celular , Humanos , Queratinocitos/metabolismo , Factor 2 Relacionado con NF-E2/metabolismo , FN-kappa B/metabolismo , Transducción de Señal/efectos de los fármacos , Factor de Transcripción AP-1/metabolismo , Rayos Ultravioleta/efectos adversos
10.
Int J Mol Sci ; 19(11)2018 Oct 25.
Artículo en Inglés | MEDLINE | ID: mdl-30366440

RESUMEN

Ultraviolet (UV) radiation induces skin photoaging, which is associated with the elevation of matrix metalloproteinases (MMPs) and the impairment of collagen. The Euphrasia species play a well-known role in the treatment of certain eye disorders through their anti-oxidative and anti-inflammatory activities. However, their protective activity toward UVB-induced damage remains unclear. In the present study, we investigated the protective effect of Euphrasia officinalis (95% ethanol extract) on UVB-irradiated photoaging in normal human dermal fibroblasts (NHDFs). Our results show that Euphrasia officinalis extract exhibited obvious reactive oxygen species (ROS) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, enhanced NHDF cell migration, and reduced UVB-induced apoptosis. The UVB-induced increases in MMP-1 and MMP-3 and decrease in type I procollagen were ameliorated by Euphrasia officinalis treatment, which worked by suppressing the mitogen-activated protein kinase (MAPK) and nuclear transcription factor activator protein 1 (AP-1) signaling pathways. Taken together, our data strongly suggest that Euphrasia officinalis ethanol extract could reduce UVB-induced photoaging by alleviating oxidative stress, proinflammatory activity, and cell apoptosis.


Asunto(s)
Euphrasia/química , Fibroblastos/efectos de los fármacos , Fibroblastos/efectos de la radiación , Extractos Vegetales/farmacología , Piel/citología , Rayos Ultravioleta , Movimiento Celular/efectos de los fármacos , Movimiento Celular/efectos de la radiación , Senescencia Celular/efectos de los fármacos , Senescencia Celular/efectos de la radiación , Colágeno Tipo I/metabolismo , Fibroblastos/citología , Metaloproteinasa 1 de la Matriz/metabolismo , Metaloproteinasa 3 de la Matriz/metabolismo , Piel/efectos de los fármacos , Piel/efectos de la radiación , Envejecimiento de la Piel/efectos de los fármacos , Envejecimiento de la Piel/efectos de la radiación
11.
Int J Syst Evol Microbiol ; 67(4): 784-788, 2017 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-27902198

RESUMEN

A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped and yellow-pigmented bacterium, designated strain THG-DN5.5T, was isolated from soil of a Rose of Sharon garden in Daejeon, South Korea. According to 16S rRNA gene sequence comparisons, strain THG-DN5.5T was found to be most closely related to Niabella yanshanensis CCBAU 05354T (97.7 % sequence similarity), Niabella ginsengisoli GR10-1T (97.0 %), 'Niabella terrae' ICM 1-15 (96.0 %), Niabella soli DSM 19437T (95.7 %) and Niabella aquatica RP-2T (95.6 %). The DNA-DNA relatedness between strain THG-DN5.5T and its phylogenetically closest neighbours was below 50.0 %. The DNA G+C content was 43.1 mol%. The major polar lipid of strain THG-DN5.5T was found to be phosphatidylethanolamine. The major fatty acids were identified as C16 : 0, iso-C15 : 0, iso-C15 : 1 G, and iso-C17 : 0 3-OH. MK-7 was the only menaquinone present. These data supported the affiliation of strain THG-DN5.5T to the genus Niabella. Strain THG-DN5.5T was distinguished from related species of the genus Niabellaby physiological and biochemical tests. In conclusion, strain THG-DN5.5T represents a novel species of the genus Niabella, for which the name Niabella hibiscisolisp. nov. is proposed. The type strain is THG-DN5.5T (=KACC 18857T=CCTCC AB 2016086T).


Asunto(s)
Bacteroidetes/clasificación , Jardines , Hibiscus/microbiología , Filogenia , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , Bacteroidetes/genética , Bacteroidetes/aislamiento & purificación , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Hibridación de Ácido Nucleico , Fosfatidiletanolaminas/química , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/química
12.
Int J Syst Evol Microbiol ; 67(6): 1703-1708, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28150574

RESUMEN

A Gram-stain-negative, facultatively anaerobic, non-motile, rod-shaped and yellow-pigmented bacterium, designated strain THG-DN6.14T, was isolated from a freshwater sample near Donghaksa temple in Daejeon, South Korea. On the basis of the results of 16S rRNA gene sequence comparisons, THG-DN6.14T was found to be most closely related to Emticicia sediminis JBR12T (99.1 % sequence similarity), Emticicia oligotrophica DSM 17448T (97.6 %), Emticicia aquatica HMF2925T (96.5 %), and Emticicia ginsengisoliGsoil 085T (94.4 %). The DNA-DNA relatedness between THG-DN6.14T and its phylogenetically closest neighbours was below 65.0 %. The DNA G+C content was 43.3 mol%. The major polar lipids were found to be phosphatidylethanolamine, an unidentified glycolipid and an unidentified aminoglycolipid. The major fatty acids were identified as C16 : 0, iso-C15 : 0, iso-C17 : 0 3-OH, and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The respiratory quinone was menaquinone MK-7. These data supported the affiliation of THG-DN6.14T to the genus Emticicia. THG-DN6.14Tcould be distinguished from related species of the genus Emticicia by physiological and biochemical tests. Therefore, the novel isolate represents a novel species, for which the name Emticicia aquatilis sp. nov. is proposed, with THG-DN6.14T (=KACC 18540T=CGMCC 1.15958T) as the type strain.


Asunto(s)
Cytophagaceae/clasificación , Agua Dulce/microbiología , Filogenia , Técnicas de Tipificación Bacteriana , Composición de Base , Cytophagaceae/genética , Cytophagaceae/aislamiento & purificación , ADN Bacteriano/genética , Ácidos Grasos/química , Glucolípidos/química , Hibridación de Ácido Nucleico , Fosfatidiletanolaminas/química , Pigmentación , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Microbiología del Suelo , Vitamina K 2/análogos & derivados , Vitamina K 2/química
13.
Int J Syst Evol Microbiol ; 66(5): 1920-1925, 2016 May.
Artículo en Inglés | MEDLINE | ID: mdl-26782356

RESUMEN

A Gram-staining-negative, strictly aerobic, rod-shaped and bright-yellow-pigmented bacterium, motile by means of a single polar flagellum, designated THG-MD21T, was isolated from rhizosphere soil of Radix ophiopogonis in Henan province, PR China. On the basis of 16S rRNA gene sequence similarity, strain THG-MD21T belongs to the genus Luteimonas and was most closely related to Luteimonas aestuarii B9T (98.2 % sequence similarity), Lysobacter panaciterrae Gsoil 068T (97.2 %) and Luteimonas marina FR1330T (97.0 %). The DNA G+C content was 64.4 mol%. The DNA-DNA relatedness between strain THG-MD21T and its closest phylogenetic neighbours was below 30.0 %. The only isoprenoid quinone detected in strain THG-MD21T was ubiquinone-8.The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and the predominant fatty acids were iso-C11 : 0, iso-C15 : 0, iso-C16 : 0, C16 : 0 and iso-C17 : 1ω9c. The DNA-DNA hybridization result and characteristics revealed by a polyphasic study showed that strain THG-MD21T represents a novel species, for which the name Luteimonas terrae sp. nov. is proposed. The type strain is THG-MD21T ( = KACC 18131T = JCM 30122T).


Asunto(s)
Filogenia , Rizosfera , Microbiología del Suelo , Xanthomonadaceae/clasificación , Técnicas de Tipificación Bacteriana , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/química , Hibridación de Ácido Nucleico , Fosfolípidos/química , Raíces de Plantas/microbiología , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Ubiquinona/química , Xanthomonadaceae/genética , Xanthomonadaceae/aislamiento & purificación
14.
Int J Syst Evol Microbiol ; 66(1): 341-346, 2016 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-26514816

RESUMEN

A bacterial isolate, designated strain THG-MM1T, was isolated from soil in Yongin region, Republic of Korea. Strain THG-MM1T was Gram-stain-negative, aerobic, non-motile and rod-shaped. Based on 16S rRNA gene sequence comparisons, strain THG-MM1T was most closely related to Devosia insulae DS-56T (96.5 % 16S rRNA gene sequence similarity), followed by Devosia yakushimensis Yak96BT (95.7 %) and Devosia albogilva IPL15T (95.2 %). The G+C content of the genomic DNA was 63.7 mol%. The major cellular fatty acids were C16 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C18 : 0. The predominant isoprenoid quinone was ubiquinone-10 (Q-10). The major polar lipids were phosphatidylglycerol and an unidentified lipid. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain THG-MM1T represents a novel species of the genus Devosia, for which the name Devosia humi sp. nov. is proposed, with strain THG-MM1T ( = KACC 18281T = CCTCC AB 2015121T) as the type strain.


Asunto(s)
Hyphomicrobiaceae/clasificación , Filogenia , Pinus/microbiología , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Hyphomicrobiaceae/genética , Hyphomicrobiaceae/aislamiento & purificación , Datos de Secuencia Molecular , Fosfolípidos/química , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Ubiquinona/química
15.
Int J Syst Evol Microbiol ; 66(11): 4729-4734, 2016 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-27515386

RESUMEN

A Gram-staining-negative, aerobic, non-motile, rod-shaped and yellow-pigmented bacterium, designated strain THG-DN6.20T, was isolated from a lotus pond near Donghaksa temple in Daejeon, Republic of Korea. According to 16S rRNA gene sequence comparisons, strain THG-DN6.20T was found to be most closely related to Novosphingobium rosa IFO 15208T (97.6 % sequence similarity), Novosphingobium sediminicola HU1-AH51T (97.5 %) and Novosphingobium barchaimii LL02T (96.9 %). The DNA-DNA relatedness between strain THG-DN6.20T and its phylogenetically closest neighbours was below 60.0 %. The respiratory quinone and polyamine detected in strain THG-DN6.20T were ubiquinone Q-10 and spermidine, respectively. The DNA G+C content was 63.1 mol%. The major polar lipids were found to be phosphatidylethanolamine, diphosphatidylglycerol, sphingoglycolipid and phosphatidylcholine. The major fatty acids were identified as C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C14 : 0 2-OH. These data supported the affiliation of strain THG-DN6.20T to the genus Novosphingobium. Strain THG-DN6.20T could be distinguished from related species of the genus Novosphingobium by physiological and biochemical characteristics. Therefore, the novel isolate represents a novel species, for which the name Novosphingobium lotistagni sp. nov. is proposed, with THG-DN6.20T as the type strain (=KACC 18541T=CCTCC AB 2015354T).


Asunto(s)
Filogenia , Estanques/microbiología , Sphingomonadaceae/clasificación , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Lotus , Hibridación de Ácido Nucleico , Fosfolípidos/química , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Sphingomonadaceae/genética , Sphingomonadaceae/aislamiento & purificación , Ubiquinona/química
16.
Arch Microbiol ; 197(6): 781-7, 2015 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-25925590

RESUMEN

A Gram-negative, yellow-colored, rod-shaped, strictly aerobic, motile bacterium was isolated from forest soil in Gyeongsang Province, South Korea. The strain was designated as THG-DA2.1(T). Cells of strain THG-DA2.1(T) grew optimally at pH 7.0, at temperature 28 °C and in the presence of 0.5 % NaCl. Based on 16S rRNA gene sequence, strain THG-DA2.1(T) was shown to belong to the genus Pedobacter and shares highest sequence similarity with Pedobacter ginsengisoli KCTC 12576(T). The DNA G+C content was determined to be 42.3 mol %. The predominant isoprenoid quinone was identified as menaquinone MK-7. The major cellular fatty acids of strain THG-DA2.1(T) were iso-C15:0 and C16:1 ω6c and/or C16:1 ω7c (summed feature 3*). Strain THG-DA2.1(T) contains phosphatidylethanolamine as major polar lipid, but an unidentified aminolipid, two unidentified phospholipids and two unidentified lipids were also detected. On the basis of the data obtained from polyphasic taxonomy in this study, strain THG-DA2.1(T) is considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter edaphicus sp. nov. is proposed. The type strain is THG-DA2.1(T) (=KCTC 42232(T) =JCM 30351(T)).


Asunto(s)
Bosques , Pedobacter/aislamiento & purificación , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/aislamiento & purificación , Ácidos Grasos/análisis , Hibridación de Ácido Nucleico , Pedobacter/genética , Fosfolípidos/análisis , Filogenia , ARN Ribosómico 16S/aislamiento & purificación , República de Corea , Análisis de Secuencia de ADN
17.
Arch Microbiol ; 197(8): 1011-7, 2015 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-26210236

RESUMEN

A Gram-reaction-negative, non-motile and rod-shaped bacterium, designated as THG-DN3.6(T), was isolated from an ancient tree trunk from Republic of Korea. On the basis of 16S rRNA gene sequence analysis, strain THG-DN3.6(T) was shown to belong to the genus Chryseobacterium and the highest similarity to Chryseobacterium indoltheticum LMG 4025(T) (97.2%) and the closest phylogenetic relatives were Chryseobacterium scophthalmum (96.8%), Chryseobacterium piscium (96.7%) and Chryseobacterium balustinum KCTC 2903(T) (96.3%). The DNA G + C content of the isolate was 33.2 mol%. The predominant isoprenoid quinone was menaquinone-6. The major fatty acids were iso-C15:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω7t and/or iso-C15:0 2-OH), iso-C17:1 ω9c and iso-C17:0 3-OH. The major polar lipids of strain THG-DN3.6(T) were phosphatidylethanolamine. The mean DNA-DNA relatedness of strain THG-DN3.6(T) to C. indoltheticum LMG 4025(T) was 52 ± 0.5%. Based on the results of polyphasic characterization, strain THG-DN3.6(T) represented a novel species within the genus Chryseobacterium, for which the name Chryseobacterium formosus sp. nov. is proposed. The type strain is THG-DN3.6(T) (=KCTC 42606 = CCTCC AB 2015118). The NCBI GenBank accession number for the 16S rRNA gene sequence of strain THG-DN3.6(T) is KM035938.


Asunto(s)
Chryseobacterium/clasificación , Árboles/microbiología , Técnicas de Tipificación Bacteriana , Composición de Base , Chryseobacterium/genética , Chryseobacterium/aislamiento & purificación , ADN Bacteriano/genética , Ácidos Grasos/análisis , Datos de Secuencia Molecular , Filogenia , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Especificidad de la Especie
18.
Int J Syst Evol Microbiol ; 65(Pt 3): 983-989, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25563919

RESUMEN

A Gram-staining-negative, catalase- and oxidase-positive, obligately aerobic, motile by gliding, bright yellow and rod-shaped bacterial strain was isolated from soil of Daemo Mountain (Daemosan) in Seoul, Republic of Korea. Its taxonomic position was investigated by using a polyphasic study. On the basis of 16S rRNA gene sequence comparisons, strain THG-DJ7(T) was found to be most closely related to Flavobacterium denitrificans ED5(T) (97.9 % sequence similarity), Flavobacterium ginsenosidimutans THG 01(T) (97.6 %), Flavobacterium kyungheensis THG-107(T) (97.5 %), Flavobacterium anhuiense D3(T) (97.1 %) and Flavobacterium ginsengisoli DCY54(T) (97.0 %). The DNA-DNA relatedness between strain THG-DJ7(T) and its phylogenetically closest neighbours was below 40.0 %. The G+C content of the genomic DNA was determined to be 32.6 mol%. The only isoprenoid quinone detected in strain THG-DJ7(T) was menaquinone-6 (MK-6). The major component in the polyamine pattern was sym-homospermidine. The major polar lipids were found to be phosphatidylethanolamine, phosphatidyldimethylethanolamine, an unidentified aminophosphoglycolipid, phosphatidylserine and an unidentified lipid. The major fatty acids were identified as iso-C15 : 0, iso-C15 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). These data support the affiliation of strain THG-DJ7(T) to the genus Flavobacterium. We report the phenotypic, genotypic and phylogenetic characterization of strain THG-DJ7(T). Based on the findings presented, a novel species of the genus Flavobacterium, Flavobacterium daemonensis sp. nov. is proposed, with THG-DJ7(T) ( = KACC 17651(T) = JCM 19455(T)) as the type strain.


Asunto(s)
Flavobacterium/clasificación , Filogenia , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Flavobacterium/genética , Flavobacterium/aislamiento & purificación , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Fosfolípidos/química , Pigmentación , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Espermidina/análogos & derivados , Espermidina/química , Vitamina K 2/análogos & derivados , Vitamina K 2/química
19.
Int J Syst Evol Microbiol ; 65(Pt 3): 1008-1014, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25563921

RESUMEN

A Gram-stain-negative, strictly aerobic, rod-shaped and pinkish-yellow bacterium, which was motile by gliding and designated strain THG-T11(T), was isolated from tomato rhizosphere soil in Gyeonggi province, Republic of Korea. Based on 16S rRNA gene sequence comparisons, strain THG-T11(T) was found to be most closely related to 'Pedobacter zeaxanthinifaciens' TDMA-5 (95.9 % sequence similarity), Pedobacter agri PB92(T) (94.9 %), Pedobacter rhizosphaerae 01-96(T) (94.6 %) and Pedobacter alluvionis NWER-II11(T) (94.5 %). The DNA G+C content was 38.4 mol%. The only isoprenoid quinone detected in strain THG-T11(T) was menaquinone-7 (MK-7). The major component in the polyamine pattern was sym-homospermidine. The major polar lipids were phosphatidylethanolamine, an unidentified phosphoglycolipid, an unidentified glycolipid, an unidentified lipid, unidentified aminophospholipids and unidentified aminolipids. The major ceramide was found to be ceramide phosphorylethanolamine. The major fatty acids were identified as iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. These data support the affiliation of strain THG-T11(T) to the genus Pedobacter. Based on phenotypic, chemotaxonomic and phylogenetic analysis, it is proposed that strain THG-T11(T) represents a novel species of the genus Pedobacter for which the name Pedobacter ureilyticus sp. nov. is proposed, with THG-T11(T) as the type strain ( = KACC 17660(T) = JCM 19461(T)).


Asunto(s)
Pedobacter/clasificación , Filogenia , Rizosfera , Microbiología del Suelo , Solanum lycopersicum/microbiología , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Datos de Secuencia Molecular , Pedobacter/genética , Pedobacter/aislamiento & purificación , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Espermidina/análogos & derivados , Espermidina/química , Vitamina K 2/análogos & derivados , Vitamina K 2/química
20.
Int J Syst Evol Microbiol ; 65(Pt 4): 1180-1185, 2015 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25604344

RESUMEN

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated THG-DD7(T), was isolated from tomato plant rhizosphere soil. Strain THG-DD7(T) grew optimally at 25-30 °C, at pH 7.0-7.5 and in the presence of 0.5% (w/v) NaCl. According to the results of 16S rRNA gene sequence comparisons, strain THG-DD7(T) was most closely related to Rhodanobacter umsongensis GR24-2(T) (98.2%), Rhodanobacter panaciterrae LnR5-47(T) (98.0%), Rhodanobacter soli DCY45(T) (97.9%), Rhodanobacter terrae GP18-1(T) (97.9%) and Dyella ginsengisoli Gsoil 3046(T) (97.7%). The DNA G+C content was 65.2 mol%. In DNA-DNA hybridization, the DNA relatedness levels between strain THG-DD7(T) and its closest phylogenetically neighbours were below 40.0%. The predominant isoprenoid quinone was ubiquinone Q-8. The major polar lipids were diphosphatidylglycerol, phosphtidylethanolamine, phosphatidyl-N-methylethanolamine and phosphatidylglycerol. The major fatty acids were iso-C(15 : 0), iso-C(16 : 0), iso-C(17 : 0), anteiso-C(15 : 0) and iso-C(17 : 1)ω9c and/or C(16 : 0) 10-methyl (summed feature 9). These data supported the affiliation of strain THG-DD7(T) to the genus Rhodanobacter . The results of physiological and biochemical tests enabled strain THG-DD7(T) to be differentiated genotypically and phenotypically from the species of the genus Rhodanobacter with validly published names. Therefore, the novel isolate represents a novel species, for which the name Rhodanobacter koreensis sp. nov. is proposed. The type strain is THG-DD7(T) ( = KACC 17650(T) = JCM 19614(T)).


Asunto(s)
Filogenia , Rizosfera , Microbiología del Suelo , Solanum lycopersicum/microbiología , Xanthomonadaceae/clasificación , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Fosfolípidos/química , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Ubiquinona/química , Xanthomonadaceae/genética , Xanthomonadaceae/aislamiento & purificación
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