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Meloxicam is routinely used for pain alleviation in pre-ruminant calves during husbandry procedures. The pharmacokinetics of a single dose (0.5 mg/kg) of meloxicam was investigated after intravenous (IV), subcutaneous (SC), and oral (PO) administration in 30 pre-ruminant calves. Each group included 10 calves. Oral meloxicam was administered at least 1 h after feeding. Plasma samples were collected for up to 168 h, and the meloxicam concentration was analysed with liquid chromatography and mass spectrometry, followed by a noncompartmental pharmacokinetic analysis. The maximum meloxicam concentrations in plasma were 1.91 ± 0.27 µg/mL and 1.77 ± 0.16 µg/mL after SC and PO routes, respectively. The time of maximum concentration was 7.6 ± 2.8 h after SC and 10.0 ± 5.7 h after PO administration. The approximate bioavailability of meloxicam was 97% for SC and PO routes. The elimination half-lives were 79.2 ± 12.4, 84.6 ± 24.8, and 84.8 ± 22.3 h after IV, SC, and PO routes, respectively. The results suggest that the therapeutic meloxicam concentrations in plasma that are required for pain relief in other species, such as horses, may be maintained for several days following a single dose (0.5 mg/kg) administered IV, SC, or PO in calves.
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Antiinflamatorios no Esteroideos , Tiazinas , Bovinos , Animales , Caballos , Meloxicam/uso terapéutico , Antiinflamatorios no Esteroideos/farmacocinética , Tiazinas/farmacocinética , Tiazoles/farmacocinética , Semivida , Área Bajo la Curva , Dolor/veterinaria , Administración Oral , RumiantesRESUMEN
OBJECTIVE: To compare the sedative effects of intramuscular xylazine alone or combined with levomethadone or ketamine in calves before cautery disbudding. STUDY DESIGN: Randomized, blinded, clinical trial. ANIMALS: A total of 28 dairy calves, aged 21 ± 5 days and weighing 61.0 ± 9.3 kg (mean ± standard deviation). METHODS: Calves were randomly allocated to three groups: xylazine (0.1 mg kg-1) and levomethadone (0.05 mg kg-1; group XL), xylazine (0.1 mg kg-1) and ketamine (1 mg kg-1; group XK) and xylazine alone (0.2 mg kg-1; group X). Local anaesthesia (procaine hydrochloride) and meloxicam were administered subcutaneously 15 minutes after sedation and 15 minutes before disbudding. The calves' responses to the administration of local anaesthesia and disbudding were recorded. Sedation was assessed at baseline and at intervals up to 240 minutes postsedation. Times of recumbency, first head lift and first standing were recorded. Drug plasma concentrations were measured. RESULTS: Data were obtained from 27 animals. All protocols resulted in sedation sufficient to administer local anaesthesia and to perform disbudding. Sedation scores significantly correlated with drug plasma concentrations (p ≤ 0.002). Times to recumbency did not differ among protocols (2.8 ± 0.3, 3.1 ± 1.1 and 2.1 ± 0.8 minutes for groups XL, XK and X, respectively), whereas interval from drug(s) administration until first head lift was significantly shorter in group XK than X (47.3 ± 14.1, 34.4 ± 5.3 and 62.6 ± 31.9 minutes for groups XL, XK and X, respectively). The area under the time-sedation curve was significantly greater in group X than XK or XL (754 ± 215, 665 ± 118 and 1005 ± 258 minutes for groups XL, XK and X, respectively). CONCLUSIONS AND CLINICAL RELEVANCE: Levomethadone or ketamine with a low dose of xylazine produced short but sufficient sedation for local anaesthesia and disbudding with minimum resistance.
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Bovinos , Cuernos , Ketamina , Anestesia Local/veterinaria , Animales , Cuernos/cirugía , Hipnóticos y Sedantes/farmacología , Ketamina/farmacología , Xilazina/farmacologíaRESUMEN
The fate of experimental animals represents an ethical dilemma and a public concern. In the EU, Directive 2010/63/EU allows the rehoming of former experimental animals instead of euthanasia. However, to our knowledge, there are no previous reports of rehoming Beagles in Finland. This study aimed to describe the process behind the first rehoming of laboratory Beagles at the University of Helsinki and evaluate its success. In total, 16 former laboratory Beagles were rehomed in collaboration with animal protection organisations and the University of Helsinki. The dogs had participated in animal cognition studies and had undergone minor procedures during the development of a veterinary drug. While the dogs were still in the laboratory, a socialisation training programme lasting several months was undertaken. Through surveying of the adoptive owners, and interviewing the various stakeholders involved (researchers, animal protection organisations and animal caretakers), the overall process was evaluated, including: the socialisation training programme; the comparative success of rehoming younger compared to older animals; the criteria that were used for the selection of the adoptive owners; and the eventual success of rehoming the dogs with the new owners. The majority of the dogs adjusted well to their new home environment. Euthanasia at the end of their experimental use would have been unnecessary and possibly against the objectives of European directives.
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Bienestar del Animal , Socialización , Animales , Perros , Finlandia , Estudios de Seguimiento , PropiedadRESUMEN
Foot pad dermatitis and hock burn lesions are a form of contact dermatitis, a condition affecting skin areas in contact with unsuitable or irritating material. Contact dermatitis is a common problem, reducing the welfare of broilers, and is believed to also affect broiler breeders. However, there is very little research on contact dermatitis in breeders. This study followed the severity of foot pad lesions in broiler breeders throughout the production period. At slaughter the presence of hock burns and breast blisters was also determined. In addition, changes in litter condition over time and the impact of litter quality on foot pads were evaluated. The study was performed on 10 broiler breeder farms, including altogether 18 flocks. Foot pads of 100 hens per flock were assessed at the end of rearing period, three times during the production period, and at slaughter. Foot pad and hock lesions, as well as litter condition were scored on a 5-point scale. Litter quality was evaluated as pH, moisture and ammonia content. The condition of foot pads deteriorated towards slaughter age, with the occurrence of severe lesions reaching a maximum of 64% on average at slaughter. Hock lesions and breast blisters were rare. The litter layer became drier over time. Although poorer litter condition and wetness influenced foot pad health negatively, the effect on severe lesions was not significant. We also observed a negative effect on foot pad condition of larger slat areas. In conclusion, maintaining good litter quality alone is not enough to ensure healthy foot pads in broiler breeders.
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Vesícula/veterinaria , Quemaduras/veterinaria , Pollos , Dermatosis del Pie/veterinaria , Vivienda para Animales , Enfermedades de las Aves de Corral/epidemiología , Crianza de Animales Domésticos , Bienestar del Animal , Animales , Vesícula/epidemiología , Vesícula/etiología , Cruzamiento , Quemaduras/epidemiología , Quemaduras/etiología , Femenino , Finlandia/epidemiología , Pisos y Cubiertas de Piso/normas , Dermatosis del Pie/epidemiología , Dermatosis del Pie/etiología , Masculino , Enfermedades de las Aves de Corral/etiología , Tarso AnimalRESUMEN
OBJECTIVE: To study the effects of oromucosal detomidine gel administered sublingually to calves prior to disbudding, and to compare its efficacy with intravenously (IV) administered detomidine. STUDY DESIGN: Randomised, prospective clinical study. ANIMALS: Twenty dairy calves aged 12.4 ± 4.4days (mean ± SD), weight 50.5 ± 9.0 kg. METHODS: Detomidine at 80 µg kg(-1) was administered to ten calves sublingually (GEL) and at 30 µg kg(-1) to ten control calves IV (V. jugularis). Meloxicam (0.5 mg kg(-1) ) and local anaesthetic (lidocaine 3 mg kg(-1) ) were administered before heat cauterization of horn buds. Heart rate (HR), body temperature and clinical sedation were monitored over 240 minutes. Blood was collected from the V. cephalica during the same period for drug concentration analysis. Pharmacokinetic variables were calculated from the plasma detomidine concentration-time data using non-compartmental methods. Statistical analyses compared routes of administration by Student's t-test and linear mixed models as relevant. RESULTS: The maximum plasma detomidine concentration after GEL was 2.1 ± 1.2 ng mL(-1) (mean ±SD) and the time of maximum concentration was 66.0 ± 36.9 minutes. The bioavailability of detomidine was approximately 34% with GEL. Similar sedation scores were reached in both groups after administration of detomidine, but maximal sedation was reached earlier in the IV group (10 minutes) than in the GEL group (40 minutes). HR was lower after IV than GEL from 5 to 10 minutes after administration. All animals were adequately sedated, and we were able to administer local anaesthetic without resistance to all of the calves before disbudding. CONCLUSIONS AND CLINICAL RELEVANCE: Oromucosally administered detomidine is an effective sedative agent for calves prior to disbudding.
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Enfermedades de los Bovinos/prevención & control , Cuernos/cirugía , Hipnóticos y Sedantes/farmacología , Imidazoles/farmacología , Dolor Postoperatorio/veterinaria , Administración Sublingual , Animales , Temperatura Corporal , Bovinos , Sedación Consciente/métodos , Sedación Consciente/veterinaria , Femenino , Geles , Frecuencia Cardíaca , Hipnóticos y Sedantes/administración & dosificación , Imidazoles/administración & dosificación , Imidazoles/sangre , Imidazoles/farmacocinética , Inyecciones Intravenosas , Masculino , Dolor Postoperatorio/prevención & controlRESUMEN
Manipulative behaviour that consists of touching or close contact with ears or tails of pen mates is common in pigs and can become damaging. Manipulative behaviour was analysed from video recordings of 45-day-old pigs, and 15 manipulator-control pairs (n = 30) were formed. Controls neither received nor performed manipulative behaviour. Rectal faecal samples of manipulators and controls were compared. 16S PCR was used to identify Lactobacillaceae species and 16S amplicon sequencing to determine faecal microbiota composition. Seven culturable Lactobacillaceae species were identified in control pigs and four in manipulator pigs. Manipulators (p = 0.02) and females (p = 0.005) expressed higher Lactobacillus amylovorus, and a significant interaction was seen (sex * status: p = 0.005) with this sex difference being more marked in controls. Females (p = 0.08) and manipulator pigs (p = 0.07) tended to express higher total Lactobacillaceae. A tendency for an interaction was seen in Limosilactobacillus reuteri (sex * status: p = 0.09). Results suggest a link between observed low diversity in Lactobacillaceae and the development of manipulative behaviour.
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Lactobacillaceae , Recto , Porcinos , Femenino , Masculino , Animales , HecesRESUMEN
Flank lesions in pigs are a common yet poorly understood consequence of damaging social behavior. One group of pigs on a commercial farm with group lactation and late weaning, and with the history of flank lesions was studied. Skin lesions on the flanks, including linear and circular lesions, and tail lesions on 69 pigs were recorded six times during 5 weeks after weaning at the age of 9 weeks. Nosing behavior was scanned during six sessions with multiple scans. The associations of age, trunk whiteness, weight gain, sow parity, litter size, sex, and tail lesions with the number of circular and linear lesions were analyzed using linear mixed models. The number of linear lesions increased as pigs aged, and pigs with a higher weight gain had more linear lesions. Moreover, pigs with a whiter trunk color were scored with more lesions of both types. According to descriptive behavior data, nosing and biting behaviors were most frequent during weeks 2-4 after weaning at the age of 11-13 weeks. On average, seven circular flank lesions were found per pig during the experiment, at the age of 10-14 weeks. After the peak on day 17, their occurrence decreased. Skin lesion occurrence was related to a lighter skin color on the trunks of pigs. We recommend reporting skin color in connection with lesion scoring results. Nosing behavior and flank lesions both peaked from 2 to 4 weeks after weaning, suggesting that nosing behavior contributed to lesion development during this time.
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The effects of bedding material on air quality are important amongst horses worldwide. Respiratory diseases, especially equine asthma, are highly prevalent with air hygiene playing a major role on the pathophysiology of these diseases. The objective of our study was to investigate the effects of four bedding materials on the respiratory signs, tracheal mucus score, and tracheal wash (TW) and bronchoalveolar lavage fluid (BALF) cytology in healthy adult horses. The study design was a prospective controlled cross-over study, and the subjects were healthy adult riding school horses (n = 32) from a single stable. Wood pellet, straw pellet, and loosely stored peat (Peat 3) were compared to peat packed in plastic-covered bales (Peat 2). Lower airway endoscopy and sampling (TW and BALF) for cytological examination were performed after each 35-day bedding period. The tracheal mucus scores (P = 0.014) and respiratory rate (P = 0.026) were higher during the straw pellet period compared to the Peat 2 period. The respiratory rate was lower during the wood pellet period compared to the Peat 2 period (P = 0.004). The TW neutrophil percentage during the straw pellet period was higher compared to the Peat 2 period (P = 0.0003). The BALF neutrophil percentage was higher during the straw pellet period (P = 0.005) and during the Peat 3 period compared to the Peat 2 period (P = 0.04). We conclude that baled peat (Peat 2) caused lower neutrophil percentages in the airway samples compared to straw pellet and loosely stored peat (Peat 3). No difference was observed between Peat 2 and wood pellet. The information gained from this study may assist veterinarians and horse owners in selecting appropriate bedding materials, especially for horses with equine asthma.
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Bedding materials affect stable air hygiene, and thus the development and exacerbation of equine asthma. There is limited knowledge concerning the effects of different types of bedding material on equine lower airway inflammation. The objective of our study was to investigate the effects of bedding materials on respiratory signs, tracheal mucus score, and lower airway cytology in healthy adult horses. The study design was a prospective controlled study, and the subjects were healthy adult riding school horses (n = 32) from a single stable. Wood shavings were compared to peat, which was used as a reference bedding material. Lower airway endoscopy and sampling (tracheal wash and bronchoalveolar lavage fluid) for cytological examination were performed after each 35-day bedding period. No difference between bedding periods was observed in the respiratory rate or tracheal mucus score. Tracheal wash neutrophil percentage with the wood shavings was higher compared to the previous (P = 0.040) or following (P = 0.0045) peat period. Bronchoalveolar lavage fluid neutrophil percentage with the wood shavings was higher compared to the following peat period (P < 0.001). We conclude that, between the two bedding materials used in this study, peat caused less neutrophilic lower airway inflammation in horses. The information gained from this study may assist veterinarians and horse owners in selecting bedding materials, especially for horses suffering from equine asthma.
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Castration is painful for calves. Castrating at an earlier age is often recommended, but little is known about how this affects the healing process or the pain experienced. We compared incision closure, swelling and pain sensitivity of beef calves surgically castrated at 3 (range 0 to 8 d; n = 16) or 73 (range 69 to 80 d; n = 15) d of age. Closure of the incision, as measured with a 5-point scale (1 = fresh wound, 5 = no longer visible), weight gain, and inflammation (skin temperature and swelling, as measured by scrotal circumference) were recorded on d 1, 3, 7, 11, 15, 18, 21, 25, 32, 39, 45, 61, and 77 after the procedure, until all incisions were fully healed. On these same days, pain sensitivity was assessed by applying a known and increasing force with von Frey hairs (0.02 to 300 g-force) at the edge of the castration wound and at a control site, approximately 2 to 5 cm anterior to the teats, until animals showed a behavioral response or the highest force was reached. The incisions of younger calves healed more quickly than older ones [fully healed, median (95% confidence interval); 39 (32 to 61) vs. 61 (61 to 77) d; P = 0.002], however, they had relatively more swelling in the days after castration (P < 0.001). Younger animals reacted to lighter pressure of von Frey hairs compared to older calves especially in the first stages of healing process (P < 0.001), and there were other signs indicative of inflammation processes in this region at this time. However, there was no difference in the control site for either age group. In addition, it took longer for older calves to recover their daily weight gain after the procedure (P < 0.001). Taken together, these results paint a mixed picture about the effects of age of surgical castration. Calves castrated soon after birth experience more tissue swelling and show more signs of pain, but their incisions heal sooner and their weight gain is less affected, when compared to animals castrated around 73 d of age.
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The treatment of cattle pain often relies upon veterinarians. The aim of this study was to qualify the influence of veterinarians skills, attitudes, and empathy on cattle pain assesment and consequently disbudding pain management. A web-based questionnaire was sent to Finnish veterinary students in either the preclinical or clinical stage, and also to production-animal practice oriented veterinarians. The questionnaire recorded demographics, statements of opinions, pain scoring of cattle conditions and procedures. Empathy towards humans (Interpersonal Reactivity Index, IRI) and reworded IRI to measure empathy towards animals were also covered. The overall response rate was approximately 40%. The association between pain and empathy scores were analyzed by Pearsons correlation, and the factors affecting pain scores and empathy towards animals analyzed using linear models. The need for pain medication of calves during disbudding was well recognized and the intention to treat such pain was very common. Higher mean scores for cattle pain were associated with greater empathy towards humans. On average, respondents' empathy towards animals was greater than towards humans, and was associated with respondents' empathy towards humans, family size and attachment to family pet.