Violacein switches off low molecular weight tyrosine phosphatase and rewires mitochondria in colorectal cancer cells.

In the last decade, emerging evidence has shown that low molecular weight protein tyrosine phosphatase (LMWPTP) not only contributes to the progression of cancer but is associated with prostate low survival rate and colorectal cancer metastasis. We report that LMWPTP favors the glycolytic profile in some tumors. Therefore, the focus of the present study was to identify metabolic enzymes that correlate with LMWPTP expression in patient samples. Exploratory data analysis from RNA-seq, proteomics, and histology staining, confirmed the higher expression of LMWPTP in CRC. Our descriptive statistical analyses indicate a positive expression correlation between LMWPTP and energy metabolism enzymes such as acetyl-CoA carboxylase (ACC) and fatty acid synthase (FASN). In addition, we examine the potential of violacein to reprogram energetic metabolism and LMWPTP activity. Violacein treatment induced a shift of glycolytic to oxidative metabolism associated with alteration in mitochondrial efficiency, as indicated by higher oxygen consumption rate. Particularly, violacein treated cells displayed higher proton leak and ATP-linked oxygen consumption rate (OCR) as an indicator of the OXPHOS preference. Notably, violacein is able to bind and inhibit LMWPTP. Since the LMWPTP acts as a hub of signaling pathways that offer tumor cells invasive advantages, such as survival and the ability to migrate, our findings highlight an unexplored potential of violacein in circumventing the metabolic plasticity of tumor cells.

Surveillance of SARS-CoV-2 immunogenicity: loss of immunodominant HLA-A*02-restricted epitopes that activate CD8+ T cells.

Introduction and methods: In this present work, coronavirus subfamilies and SARS-CoV-2 Variants of Concern (VOCs) were investigated for the presence of MHC-I immunodominant viral peptides using in silico and in vitro tools. Results: In our results, HLA-A*02 haplotype showed the highest number of immunodominant epitopes but with the lowest combined prediction score. Furthermore, a decrease in combined prediction score was observed for HLA-A*02-restricted epitopes when the original strain was compared to the VOCs, indicating that the mutations on the VOCs are promoting escape from HLA-A2-mediated antigen presentation, which characterizes a immune evasion process. Additionally, epitope signature analysis revealed major immunogenic peptide loss for structural (S) and non-structural (ORF8) proteins of VOCs in comparison to the Wuhan sequence. Discussion: These results may indicate that the antiviral CD8+ T-cell responses generated by original strains could not be sufficient for clearance of variants in either newly or reinfection with SARS-CoV-2. In contrast, N epitopes remain the most conserved and reactive peptides across SARS-CoV-2 VOCs. Overall, our data could contribute to the rational design and development of new vaccinal platforms to induce a broad cellular CD8+ T cell antiviral response, aiming at controlling viral transmission of future SARS-CoV-2 variants.

In silico and in vitro arboviral MHC class I-restricted-epitope signatures reveal immunodominance and poor overlapping patterns.

Introduction: The present work sought to identify MHC-I-restricted peptide signatures for arbovirus using in silico and in vitro peptide microarray tools. Methods: First, an in-silico analysis of immunogenic epitopes restricted to four of the most prevalent human MHC class-I was performed by identification of MHC affinity score. For that, more than 10,000 peptide sequences from 5 Arbovirus and 8 different viral serotypes, namely Zika (ZIKV), Dengue (DENV serotypes 1-4), Chikungunya (CHIKV), Mayaro (MAYV) and Oropouche (OROV) viruses, in addition to YFV were analyzed. Haplotype HLA-A*02.01 was the dominant human MHC for all arboviruses. Over one thousand HLA-A2 immunogenic peptides were employed to build a comprehensive identity matrix. Intending to assess HLAA*02:01 reactivity of peptides in vitro, a peptide microarray was designed and generated using a dimeric protein containing HLA-A*02:01. Results: The comprehensive identity matrix allowed the identification of only three overlapping peptides between two or more flavivirus sequences, suggesting poor overlapping of virus-specific immunogenic peptides amongst arborviruses. Global analysis of the fluorescence intensity for peptide-HLA-A*02:01 binding indicated a dose-dependent effect in the array. Considering all assessed arboviruses, the number of DENV-derived peptides with HLA-A*02:01 reactivity was the highest. Furthermore, a lower number of YFV-17DD overlapping peptides presented reactivity when compared to non-overlapping peptides. In addition, the assessment of HLA-A*02:01-reactive peptides across virus polyproteins highlighted non-structural proteins as "hot-spots". Data analysis supported these findings showing the presence of major hydrophobic sites in the final segment of non-structural protein 1 throughout 2a (Ns2a) and in nonstructural proteins 2b (Ns2b), 4a (Ns4a) and 4b (Ns4b). Discussion: To our knowledge, these results provide the most comprehensive and detailed snapshot of the immunodominant peptide signature for arbovirus with MHC-class I restriction, which may bring insight into the design of future virus-specific vaccines to arboviruses and for vaccination protocols in highly endemic areas.

Human cytomegalovirus reinfection is associated with intrauterine transmission in a highly cytomegalovirus-immune maternal population.

OBJECTIVE: To determine contribution of reinfection with new strains of cytomegalovirus in cytomegalovirus seromimmune women to incidence of congenital cytomegalovirus infection. STUDY DESIGN: In 7848 women studied prospectively for congenital cytomegalovirus infection from a population with near universal cytomegalovirus seroimmunity, sera from 40 mothers of congenitally infected infants and 109 mothers of uninfected newborns were analyzed for strain-specific anticytomegalovirus antibodies. RESULTS: All women were cytomegalovirus seroimmune at first prenatal visit. Reactivity for 2 cytomegalovirus strains was found in 14 of 40 study mothers and in 17 of 109 control mothers at first prenatal visit (P = .009). Seven of 40 (17.5%) study women and 5 of 109 (4.6%) controls (P = .002) acquired antibodies reactive with new cytomegalovirus strains during pregnancy. Evidence of infection with more than 1 strain of cytomegalovirus before or during current pregnancy occurred in 21 of 40 study mothers and 22 of 109 controls (P < .0001). CONCLUSION: Maternal reinfection by new strains of cytomegalovirus is a major source of congenital infection in this population.

Correlation analysis of the visual-analogue scale and the Tinnitus Handicap Inventory in tinnitus patients.

UNLABELLED: One of the most challenging topics in tinnitus clinical studies is the measuring method used. Visual Analogue Scales (VAS) and Tinnitus Handicap Inventory (THI) are frequently used in tinnitus. AIM: To verify the relationship between VAS and THI scores in tinnitus patients in a prospective study. MATERIALS AND METHODS: 43 patients classified their tinnitus according to VAS and THI, and both scores were compared through the Spearman's correlation coefficient test. RESULTS: There was a correlation between the VAS and THI scores. CONCLUSION: There is correlation between VAS and THI scores in patients with sensorineural tinnitus.

Characterization of Cardiopulmonary Exercise Testing Variables in Patients with Endomyocardial Fibrosis after Endocardial Resection.

BACKGROUND: Endomyocardial fibrosis (EMF) is a rare disease, characterized by diastolic dysfunction which leads to reduced peak oxygen consumption (VO2). Cardiopulmonary exercise testing (CPET) has been proved to be a fundamental tool to identify central and peripheral alterations. However, most studies prioritize peak VO2 as the main variable, leaving aside other important CPET variables that can specify the severity of the disease and guide the clinical treatment. OBJECTIVE: The aim of this study was to evaluate central and peripheral limitations in symptomatic patients with EMF by different CPET variables. METHODS: Twenty-six EMF patients (functional class III, NYHA) were compared with 15 healthy subjects (HS). Functional capacity was evaluated using CPET and diastolic and systolic functions were evaluated by echocardiography. RESULTS: Age and gender were similar between EMF patients and HS. Left ventricular ejection fraction was normal in EMF patients, but decreased compared to HS. Peak heart rate, peak workload, peak VO2, peak oxygen (O2) pulse and peak pulmonary ventilation (VE) were decreased in EMF compared to HS. Also, EMF patients showed increased Δ heart rate /Δ oxygen uptake and Δ oxygen uptake /Δ work rate compared to HS. CONCLUSION: Determination of the aerobic capacity by noninvasive respiratory gas exchange during incremental exercise provides additional information about the exercise tolerance in patients with EMF. The analysis of different CPET variables is necessary to help us understand more about the central and peripheral alterations cause by both diastolic dysfunction and restrictive pattern.

Physical capacity, respiratory and peripheral muscle strength in heart failure / Capacidade física, força muscular respiratória e periférica na insuficiência cardíaca / Capacidad física, fuerza muscular respiratoria y periférica en insuficiencia cardíaca

ABSTRACT Introduction The Glittre-ADL test is a submaximal test that involves sitting/lifting, stair climbing/descending, and weight-bearing activities, with considerable potential for use in assessing exercise intolerance in heart failure. Objectives To investigate the influence of heart failure on maximal physical (ergometric test - ET) and functional (6MWT and Glittre-ADL test) capacity, on respiratory (IPmax and EPmax) and peripheral (handgrip strength) muscle strength, and to study the correlation between the variables evaluated. Methods A prospective comparative study that assessed 44 subjects divided into two groups: G1, with 26 heart patients (20 men/6 women, 65.26 ± 8.50 years), and G2, with 18 healthy subjects paired with G1 on the basis of sex ratio, age and anthropometric characteristics (10 men/8 women, 60.72 ± 8.39 years). The comparison of categorical variables was performed using the chi-square test, while continuous variables were compared using the Student t test or Mann-Whitney test. The study of the correlation between variables was conducted using the Pearson coefficient for EPmax and handgrip strength, while the Spearman correlation coefficient was used for the others, with a statistical significance level of 5%. Results Groups were homogeneous in terms of sex ratio, age and anthropometric characteristics. G1 had a worse performance in all tests: ET (p = 0.016), Glittre-ADL (p = 0.001), 6MWT (p = 0.001), IPmax (p = 0.012), EPmax (p = 0.007) and handgrip strength (p = 0.036). There was a negative correlation between the Glittre-ADL test and 6MWT, and the Glittre-ADL test and handgrip strength in G1. Conclusion Heart failure resulted in impairment of maximal physical and functional capacity and of respiratory and peripheral muscle strength, with a negative correlation between functional capacity and handgrip strength. Level of evidence II; Prospective comparative study.

RESUMO Introdução O teste AVD-Glittre é um teste submáximo que envolve atividades de sentar/levantar, subir/descer degraus e sustentação de pesos, que tem grande potencial de uso na avaliação da intolerância ao esforço na insuficiência cardíaca. Objetivos Pesquisar a influência da insuficiência cardíaca na capacidade física máxima (teste ergométrico - TE) e funcional (TC6 e teste AVD-Glittre), na força dos músculos respiratórios (PImáx e PEmáx) e periféricos (força de preensão palmar) e estudar a correlação entre as variáveis avaliadas. Métodos Estudo prospectivo comparativo que avaliou 44 indivíduos divididos em dois grupos: G1, com 26 cardiopatas (20 homens/ 6 mulheres, 65,26 ± 8,50 anos) e G2 com 18 indivíduos saudáveis pareados com o G1 quanto à distribuição de sexo, idade e características antropométricas (10 homens/8 mulheres, 60,72 ± 8,39 anos). A comparação das variáveis categóricas foi realizada pelo teste do qui-quadrado e a das variáveis contínuas, pelo teste t de Student ou Mann-Whitney. O estudo da correlação entre variáveis foi feito pelo índice de Pearson para PEmáx e força de preensão palmar; para as demais, pelo índice de correlação de Spearman, com nível de significância estatístico de 5%. Resultados Os grupos foram homogêneos quanto à distribuição de sexo, idade e características antropométricas. O G1 apresentou pior desempenho em todos os testes: TE (p = 0,016), AVD-Glittre (p = 0,001), TC6' (p = 0,001), PImáx (p = 0,012), PEmáx (p = 0,007) e força de preensão palmar (p = 0,036). Houve correlação negativa entre AVD-Glittre e TC6 e AVD-Glittre e força de preensão palmar em G1. Conclusão A insuficiência cardíaca resultou em comprometimento da capacidade física máxima, funcional e da força muscular respiratória e periférica, com correlação negativa entre capacidade funcional e força de preensão palmar. Nível de Evidência II; Estudo Prospectivo e Comparativo.

RESUMEN Introducción La prueba AVD-Glittre es una prueba submáxima que implica sentarse/pararse, subir/bajar escalones y actividades con carga de peso, que tiene un gran potencial para uso en la evaluación de la intolerancia al estrés en la insuficiencia cardíaca. Objetivos Investigar la influencia de la insuficiencia cardíaca en la capacidad física máxima (prueba ergométrica - PE) y funcional (prueba 6MWT y AVD-Glittre), en la fuerza de los músculos respiratorios (PImáx y PEmáx) y periféricos (fuerza de agarre manual) y estudiar la correlación entre las variables evaluadas. Métodos Estudio prospectivo comparativo que evaluó a 44 individuos, divididos en dos grupos: G1, con 26 cardíacos (20 hombres/6 mujeres, 65,26 ± 8,50 años) y G2 con 18 individuos sanos emparejados con G1 con respecto a la distribución de sexo, edad y características antropométricas (10 hombres/8 mujeres, 60,72 ± 8,39 años). La comparación de las variables categóricas se realizó utilizando la prueba de chi-cuadrado y la de variables continuas, utilizando la prueba t de Student o Mann-Whitney. El estudio de la correlación entre variables se realizó utilizando el índice de Pearson para PEmáx y fuerza de agarre manual; para las demás, se utilizó el índice de correlación de Spearman, con un nivel de significación estadística del 5%. Resultados Los grupos fueron homogéneos en cuanto a la distribución por sexo, edad y características antropométricas. El G1 presentó el peor desempeño en todas las pruebas: PE (p = 0,016), AVD-Glittre (p = 0,001), 6MWT (p = 0,001), PImáx (p = 0,012), PEmáx (p = 0,007) y fuerza de agarre manual (p = 0,036). Hubo una correlación negativa entre AVD-Glittre y 6MWT y AVD-Glittre y la fuerza de agarre manual en G1. Conclusión La insuficiencia cardíaca resultó en un deterioro de la fuerza muscular máxima, funcional y respiratoria y periférica, con una correlación negativa entre la capacidad funcional y la fuerza de agarre manual. Nivel de Evidencia II; Estudio Prospectivo y Comparativo.

Characterization of Cardiopulmonary Exercise Testing Variables in Patients with Endomyocardial Fibrosis after Endocardial Resection / Caracterização das Variáveis do Teste de Esforço Cardiopulmonar em Pacientes com Endomiocardiofibrose após Cirurgia de Ressecção Endocárdica

Abstract Background: Endomyocardial fibrosis (EMF) is a rare disease, characterized by diastolic dysfunction which leads to reduced peak oxygen consumption (VO2). Cardiopulmonary exercise testing (CPET) has been proved to be a fundamental tool to identify central and peripheral alterations. However, most studies prioritize peak VO2 as the main variable, leaving aside other important CPET variables that can specify the severity of the disease and guide the clinical treatment. Objective: The aim of this study was to evaluate central and peripheral limitations in symptomatic patients with EMF by different CPET variables. Methods: Twenty-six EMF patients (functional class III, NYHA) were compared with 15 healthy subjects (HS). Functional capacity was evaluated using CPET and diastolic and systolic functions were evaluated by echocardiography. Results: Age and gender were similar between EMF patients and HS. Left ventricular ejection fraction was normal in EMF patients, but decreased compared to HS. Peak heart rate, peak workload, peak VO2, peak oxygen (O2) pulse and peak pulmonary ventilation (VE) were decreased in EMF compared to HS. Also, EMF patients showed increased Δ heart rate /Δ oxygen uptake and Δ oxygen uptake /Δ work rate compared to HS. Conclusion: Determination of the aerobic capacity by noninvasive respiratory gas exchange during incremental exercise provides additional information about the exercise tolerance in patients with EMF. The analysis of different CPET variables is necessary to help us understand more about the central and peripheral alterations cause by both diastolic dysfunction and restrictive pattern.

Resumo Fundamento: A endomiocardiofibrose (EMF) é uma doença rara, caracterizada por disfunção diastólica que leva à redução consumo de oxigênio (VO2) pico. O teste de esforço cardiopulmonar (TECP) tem se mostrado uma ferramenta fundamental na identificação de alterações centrais e periféricas. No entanto, a maioria dos estudos prioriza o VO2 pico como a variável principal, em detrimento de outras importantes variáveis do TECP que poderiam identificar a gravidade da doença e direcionar o tratamento clínico. Objetivo: O objetivo deste estudo foi avaliar limitações centrais e periféricas em pacientes com EMF sintomáticos por meio de variáveis do TECP. Métodos: Vinte e seis pacientes com EMF (classe funcional III, NYHA) foram comparados com 15 indivíduos controle saudáveis (CS). A capacidade funcional foi avaliada por TECP e funções sistólicas e diastólicas por ecocardiografia. Resultados: A idade e o gênero foram similares entre pacientes com EMF e CS. A fração de ejeção do ventrículo esquerdo foi normal em pacientes com EMF, porém diminuída em comparação aos CS. Os picos de frequência cardíaca, carga de trabalho, VO2, pulso de oxigênio (O2) e da ventilação pulmonar (VE) estavam diminuídos em pacientes com EMF em comparação aos CS. Ainda, os pacientes com EMF apresentaram Δ frequência cardíaca /Δ consumo de oxigênio e Δ consumo de oxigênio /Δ taxa de trabalho aumentados em comparação aos CS. Conclusão: A determinação da capacidade aeróbica por troca respiratória não invasiva durante exercício progressivo fornece informações sobre a tolerância ao exercício em pacientes com EMF. É necessária uma análise das diferentes variáveis do TECP para nos ajudar a compreender mais acerca das alterações centrais e periféricas causadas tanto pela disfunção diastólica como pelo padrão restritivo.

A dor e desconforto na prática do aleitamento materno / El dolor y molestia en la práctica del amamantamiento materno / Pain and discomfort in the practice of breastfeeding

A amamentação é uma prática de promoção à saúde e o conhecimento de fatores associados à interrupção é fundamental para aumentar o período da prática. Este estudo observacional, descritivo, transversal teve por objetivo conhecer os fatores de dor e de desconforto na prática do aleitamento materno. A população foi composta de mães de recém-nascidos, de dois a quatro meses, entre fevereiro e julho de 2012. Foram aplicados questionários em visitas domiciliares após a aprovação do Comitê de Ética. A idade média das nutrizes foi de 25±5,9, 26,2% eram mães adolescentes. A posição preferida referida para amamentar é a sentada e o local de dor e de desconforto foi a mama. O sexo da criança teve significância estatística (p=0,038) em relação à dor e ao desconforto materno. Ações durante o pré-natal, sobre a técnica correta para evitar dor e desconforto, devem ser incentivadas evitando o desmame precoce.

El amamantamiento es una práctica de promoción a la salud y el conocimiento de factores asociados a la interrupción es fundamental para aumentar la duración de esta práctica. Este estudio observacional, descriptivo, transversal tuvo por objetivo conocer los factores de dolor y molestia en la práctica del amamantamiento materno. La población fue compuesta de madres de recién nacidos, de dos a cuatro meses, entre febrero y julio de 2012. Fueron aplicados cuestionarios en visitas domiciliares después de la autorización del Comité de Ética. La edad media de las nutrices fue de 25±5,9; 26,2% eran madres adolescentes. La posición preferida para amamantar es la sentada y el local de dolor y molestia fue la mama. El sexo del niño tuvo significancia estadística (p=0,038) acerca del dolor y de la molestia materna. Acciones durante el prenatal, sobre la técnica correcta para evitar dolor y molestias, deben ser incentivadas evitando el desmame precoz.

Breastfeeding is a practice which promotes health, and knowledge of the factors associated with its interruption is fundamental for increasing the period of its practice. This observational, descriptive and cross-sectional study aimed to investigate the factors of pain and discomfort in the practice of breastfeeding. The population was made up of mothers of newborns aged between two and four months, between February and July 2012. Questionnaires were administered during a home visit following the approval of the Ethic Committee. The breastfeeding mothers' mean age was 25±5.9, and 26.2% were adolescent mothers. The favourite position for breastfeeding mentioned was sat down, and the place most mentioned for pain and discomfort was the breast. The child's sex had statistical significance (p=0.038) in relation to the mother's pain and discomfort. So as to avoid early weaning, actions must be encouraged during the prenatal period regarding the correct technique for avoiding pain and discomfort.

Análise da correlação entre a escala visual-análoga e o Tinnitus Handicap Inventory na avaliação de pacientes com zumbido / Correlation analysis of the visual-analogue scale and the Tinnitus Handicap Inventory in tinnitus patients

Um dos tópicos mais questionado nos estudos clínicos sobre zumbido é o método de mensuração do mesmo. As Escalas Visual-Análogas (EVAs) e o Tinnitus Handicap Inventory (THI) são freqüentemente utilizados para este fim. OBJETIVO: Verificar a correlação entre os escores da EVA e do THI em pacientes com zumbido neurossensorial através de um estudo prospectivo. MATERIAL E MÉTODO: 43 pacientes com zumbido neurossensorial quantificaram o zumbido pelos dois métodos, sendo os escores comparados através do Coeficiente de Relação de Spearman. RESULTADOS: Foi observada correlação entre os escores da EVA e do THI. CONCLUSÃO: Em pacientes com zumbido neurossensorial existe correlação entre os escores da EVA e do THI.

One of the most challenging topics in tinnitus clinical studies is the measuring method used. Visual Analogue Scales (VAS) and Tinnitus Handicap Inventory (THI) are frequently used in tinnitus. AIM: To verify the relationship between VAS and THI scores in tinnitus patients in a prospective study. MATERIALS AND METHODS: 43 patients classified their tinnitus according to VAS and THI, and both scores were compared through the Spearman's correlation coefficient test. RESULTS: There was a correlation between the VAS and THI scores. CONCLUSION: There is correlation between VAS and THI scores in patients with sensorineural tinnitus.

Repercussões do abuso sexual incestuoso sobre a relação mãe X filha / Efectos del abuso sexual incestuoso de la madre y la hija / Repercussions of incestuous sexual abuse on the mother/daugther

Esta pesquisa buscou compreender a relação entre mães e filhas após a descoberta e denúncia do abuso sexual incestuoso sofrido pela criança. Foram entrevistadas quatro mulheres que denunciaram o abuso incestuoso sofrido pelas filhas. Suas falas foram submetidas à análise de conteúdo. Os resultados demonstram que essas mulheres são muito exigentes em relação a si mesmas quanto ao papel de mães e conduzem as relações com suas filhas numa tentativa constante de protegê-las. Esse desejo de proteger a filha é tão forte, que dificulta a construção de novos relacionamentos amorosos. Todas apresentam medo de confiar novamente em um parceiro, tendo em vista o temor de que ele possa se tornar um novo agressor de sua filha. Isso permite pensar na dificuldade dessas mulheres de viver novos relacionamentos amorosos ou mesmo conviver com outros homens em espaços comuns.

This research aimed to understand the relationship between mothers and daughters after the discovery and denouncement of incestuous abuse suffered by the child. Four women who have denounced the abuse suffered by their daughters were interviewed. Their speeches were submitted to Content Analysis. Results show that those women are very strict about their mother role, and conduct the relationship with their daughters in a constant attempt to protect them. This wish to protect their daughters is so strong that it makes it harder to build new loving relationships. All of them show fear in trusting someone again, fearful that the partner can become a new aggressor of their daughters. This allows one to think about the difficulties those women have in living new loving relationships or even in dealing with other men who share common spaces with them.

Esa investigación intentó comprender la relación madre-hija después de la descubierta y denuncia del abuso incestuoso sufrido por la niña. Fueron entrevistadas cuatro mujeres que han denunciado el abuso incestuoso sufrido por sus hijas. Sus hablas fueran sometidas al Análisis de Contenido. Los resultados demuestran que esas mujeres son muy exigentes consigo mismas en cuanto al rol de madres y conducen las relaciones con sus hijas intentando constantemente protegerlas. Este deseo de proteger a su hija es tan intenso que dificulta que ellas se construyan nuevas relaciones amorosas. Todas presentan miedo de confiar de nuevo en uno compañero, tiendo en vista el temor de que este se vuelva en uno nuevo agresor de su hija. Esto permite pensar en la dificultad de esas mujeres en vivenciar nuevas relaciones amorosas o mismo convivir con otros hombres en espacios comunes.

Aspectos da predação entre larvas de Chrysoperla externa (Hagen, 1861) e Ceraeochrysa cubana (Hagen, 1861) (Neuroptera: Chrysopidae) em laboratório / Predation among Chrysoperla externa (Hagen, 1861) and Ceraeochrysa cubana (Hagen, 1861) (Neuroptera: Chrysopidae) larvae under laboratory conditions

Objetivou-se no presente trabalho estudar aspectos da predação entre larvas de Chrysoperla externa e Ceraeochrysa cubana, sob condições de laboratório, verificando a sobrevivência desses predadores nos diferentes ínstares, quando mantidos nas proporções de 1:1, 2:2 e 3:3. De modo geral, C. externa apresentou maior sobrevivência, independente do ínstar e da proporção de adensamento entre indivíduos. Ao contrário, C. cubana foi fortemente afetada pela interação com C. externa, tendo sua capacidade de sobrevivência reduzida com o aumento da proporção de adensamento e com o desenvolvimento larval. No final da fase larval, verificou-se uma taxa de sobrevivência próxima a 96, 90 e 95 por cento, e 56, 23 e 9 por cento para C. externa e C. cubana nas proporções de 1:1, 2:2 e 3:3 indivíduos, respectivamente.

This work aimed to study aspects of predation among Chrysoperla externa and Ceraeochrysa cubana larvae under laboratory conditions. The survival of those predators in different larval stages was studied, maintaining the larvae grouped in proportions of 1:1, 2:2 and 3:3 individuals. C. externa presented higher survival rates, independently of larval ínstar and density proportion among individuals. On the other hand, C. cubana was strongly affected by interaction with C. externa, and had its survival capacity reduced with the increase of grouping proportion and larval development. At the end of larval phase, a survival rate around 96, 90, and 95 and 56, 23 and 9 percent for C. externa and C. cubana in proportions of 1:1, 2:2 and 3:3 individuals, respectively, was verified.