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Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B.

Takahashi, Yukitoshi; Nishimura, Shigeko; Takao, Emiko; Kasai, Risa; Enokida, Kaoru; Ida, Kuniko; Fukuoka, Masataka; Koike, Takayoshi; Omatsu, Hiroo; Yamaguchi, Tokito; Takano, Shiho; Yoshida, Tomoyuki; Mori, Hisashi.

J Neuroimmunol ; 349: 577427, 2020 12 15.

Artículo en Inglés | MEDLINE | ID: mdl-33091764

RESUMEN

To characterize internalization of NMDA-type glutamate receptors (GluRs) by antibodies to NMDA-type GluRs, we produced rabbit antibodies to N-terminals of human GluN1 and GluN2B, and examined internalization of NMDA-type GluRs in HEK293T cells using confocal microscopy. Internalization of NMDA-type GluRs occurred from at least 10 min after incubation with antibodies to GluN1 and or GluN2B and was temperature-dependent. These findings confirm that antibodies to N-terminals of GluN1 and GluN2B present in the cerebrospinal fluid of patients with NMDAR encephalitis can mediate prompt internalization of NMDA-type GluR complexes.

Asunto(s)

Autoanticuerpos/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Secuencia de Aminoácidos , Animales , Autoanticuerpos/genética , Células HEK293 , Humanos , Proteínas del Tejido Nervioso/genética , Conejos , Receptores de Glutamato/genética , Receptores de Glutamato/metabolismo , Receptores de N-Metil-D-Aspartato/genética

RESUMEN

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