RESUMEN
In this study, the growth hormone (GH) gene was studied as a candidate gene for growth and carcass traits of three duck populations (Cherry Valley duck, Muscovy duck and Jingjiang duck). Three pairs of primers were designed to detect single nucleotide polymorphisms of introns 2, 3 and 4 of the GH gene by polymerase chain reaction-restriction fragment length polymorphism and sequencing methods. Only the products amplified from intron 2 displayed polymorphism. The results showed one novel polymorphism: a variation in intron 2 of GH gene (C172T, JN408701 and JN408702). It was associated with some growth and carcass traits in three duck populations including birth weight, 8-week weight, carcass weight, breast muscle weight, leg muscle weight, eviscerated weight, lean meat rate, dressing percentage, etc. And the TT and CT genotypes were associated with superior growth and carcass traits in carcass weight, dressing percentage and percentage of eviscerated weight. Therefore, the variation in intron 2 of GH may be a molecular marker for superior growth and carcass traits in above duck populations.
Asunto(s)
Patos/crecimiento & desarrollo , Patos/genética , Hormona del Crecimiento/genética , Polimorfismo de Nucleótido Simple , Carácter Cuantitativo Heredable , Alelos , Animales , Secuencia de Bases , Genotipo , Intrones , Datos de Secuencia Molecular , Tamaño de los ÓrganosRESUMEN
Myogenic differentiation 1 (MyoD1) genes belong to the MyoD gene family and play key roles in growth and muscle development. This study was designed to investigate the effects of variants in the MyoD1 gene on duck growth and carcass traits. Three duck populations (Cherry Valley, Jingjiang, and Muscovy) were sampled, their growth and carcass traits were measured, and they were genotyped using the PCR-RFLP method. The results showed one novel polymorphism, an alteration in intron 2 of the MyoD1 gene (A to T). It was associated with the traits of weight at 8 weeks, carcass weight, breast muscle weight, leg muscle weight, eviscerated percentage, percentage of leg muscle weight, dressing percentage, and lean meat percentage. This alteration in intron 2 of MyoD1 may be linked with potential major loci or genes affecting some growth and carcass traits.
Asunto(s)
Patos/genética , Estudios de Asociación Genética/métodos , Intrones , Proteína MioD/genética , Polimorfismo de Nucleótido Simple , Alelos , Animales , Peso Corporal , Patos/crecimiento & desarrollo , Frecuencia de los Genes , Genética de Población/métodos , Técnicas de Genotipaje , Carne , Músculos/citología , Proteína MioD/metabolismo , Fenotipo , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Carácter Cuantitativo HeredableRESUMEN
Polycrystalline CdSSe nanowires were prepared with a low-temperature physical evaporation method. Structural analysis combining HRTEM with XRD demonstrate that these as-prepared wires have a hexagonal wurtzite structure with a polycrystalline nature. Locally excited optical measurements show that though these wires can still act as waveguide cavities, their polycrystalline nature will induce a significant redshift of the emitted light during its transportation along them. Power dependent photoluminescence measurement shows that these polycrystalline wires can achieve broadband coherent emission at the band-edge band under high excitation, which shows marked contrast with the much narrower and multimode spectra observed in the single-crystalline nanowires with the same elemental composition. Time-resolved photoluminescence further confirms the occurrence of coherent emission in these wires, which originates from the electron-hole plasma (EHP) recombination of high-density carriers generated under high excitation. These kinds of polycrystalline alloy nanowires with broadband coherent emission should have potential uses in nano-scaled wavelength tunable light-emitting devices.