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1.
Food Chem ; 460(Pt 2): 140564, 2024 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-39089015

RESUMEN

Eucommia ulmoides, a plant native to China, is valued for its medicinal properties and has applications in food, health products, and traditional Chinese medicine. Processed Eucommiae Cortex (EC) has historically been a highly valued medicine. Ancient doctors had ample experience processing EC, especially with ginger juice, as documented in traditional Chinese medical texts. The combination of EC and ginger juice helps release and transform the active ingredients, strengthening the medicine's effectiveness and improving its taste and shelf life. However, the lack of quality control standards for Ginger-Eucommiae Cortex (G-EC), processed from EC and ginger, presents challenges for its industrial and clinical use. This study optimized G-EC processing using the CRITIC and Box-Behnken methods. Metabolomics showed 517 chemical changes between raw and processed G-EC, particularly an increase in coniferyl aldehyde (CFA). Explainable artificial intelligence techniques revealed the feasibility of using color to CFA content, providing insights into quality indicators.


Asunto(s)
Inteligencia Artificial , Eucommiaceae , Metabolómica , Eucommiaceae/química , Eucommiaceae/metabolismo , Color , Aldehídos/análisis , Aldehídos/metabolismo , Aldehídos/química , Manipulación de Alimentos , Extractos Vegetales/química , Extractos Vegetales/metabolismo , Zingiber officinale/química , Zingiber officinale/metabolismo
2.
Behav Sci (Basel) ; 13(10)2023 Sep 27.
Artículo en Inglés | MEDLINE | ID: mdl-37887452

RESUMEN

(1) Objective: While recent studies have shed light on the effects of shame memories on mental well-being, there is still limited understanding of the underlying mechanisms linking shame memories and depression. Based on the biopsychosocial model and cognitive therapy theory, this study examined the association between shame memory and depression and the indirect role of emotion management and self-criticism. (2) Methods: A total of 1004 junior high school students were measured with the Center for Epidemiologic Studies Depression Scale, the event impact scale, the self-criticism scale of the depressive experiences questionnaire, and the emotional intelligence scale. (3) Results: Shame memory had a significant positive correlation with depression. The relationship between shame memory and depression was partially mediated by self-criticism. Emotional management was found to regulate the latter half of the mediating effect of shame memory on depression. (4) Conclusions: Self-criticism plays a mediating role in the relationship between shame memory and depression, as well as emotion management plays a moderating role between self-criticism and depression. This perspective will contribute to the growing body of knowledge about the impact of shame memories on depression among junior high school children but also offers a feasible plan for follow-up intervention.

3.
Eur J Pharmacol ; 959: 176082, 2023 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-37783303

RESUMEN

Andrographolide (AGP) exerts pharmacological effects when used for the treatment of cardiovascular disease, but the molecular mechanisms underlying its inhibitory effects on the proliferation and migration of vascular smooth muscle cells (VSMCs) and intimal hyperplasia (IH) are unknown. The proliferation and migration of VSMCs treated with AGP were examined using the CCK-8, flow cytometry, and wound healing assays. Expression levels of proteins related to cell proliferation and apoptosis were quantified. Multi-omics analysis with RNA-seq and metabolome was used to explore the potential molecular mechanism of AGP treatment. Additionally, an in vivo model was established through ligation of the left common carotid artery to identify the therapeutic potential of AGP in IH. Molecular docking and western blotting were performed to verify the mechanism discovered with multi-omics analysis. The results showed that AGP inhibited the proliferation and migration of cultured VSMCs in a dose-dependent manner and alleviated IH-related vascular stenosis. AGP significantly downregulated the protein levels of CDK1, CCND1, and BCL2 and upregulated the protein level of BAX. Gene expression profiles showed a total of 3,298 differentially expressed genes (DEGs) after AGP treatment, of which 1,709 DEGs had upregulated expression and 1,589 DEGs had downregulated expression. KEGG enrichment analysis highlighted the PI3K/AKT signaling pathway, verified with the detection of the activation of PI3K and AKT phosphorylation. Further GO enrichment combined with metabolomics analysis showed that AGP inhibition in cultured VSMCs involved the amino acid metabolic process, and the expression levels of the two key factors PRDM16 and EZH2, identified with PPI and docking analysis, were significantly inhibited by AGP treatment. In conclusion, our study showed that AGP inhibited VSMCs proliferation and migration by suppressing the PI3K/AKT signaling pathway and amino acid metabolism, which, in turn, improved IH.


Asunto(s)
Fosfatidilinositol 3-Quinasas , Proteínas Proto-Oncogénicas c-akt , Humanos , Proteínas Proto-Oncogénicas c-akt/metabolismo , Fosfatidilinositol 3-Quinasas/metabolismo , Músculo Liso Vascular , Hiperplasia/patología , Simulación del Acoplamiento Molecular , Transducción de Señal , Proliferación Celular , Aminoácidos/farmacología , Miocitos del Músculo Liso , Movimiento Celular , Células Cultivadas
4.
Front Plant Sci ; 14: 1145624, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37063183

RESUMEN

Cultivated peanut (Arachis hypogaea) is a leading protein and oil-providing crop and food source in many countries. At the same time, it is affected by a number of biotic and abiotic stresses. O-methyltransferases (OMTs) play important roles in secondary metabolism, biotic and abiotic stress tolerance. However, the OMT genes have not been comprehensively analyzed in peanut. In this study, we performed a genome-wide investigation of A. hypogaea OMT genes (AhOMTs). Gene structure, motifs distribution, phylogenetic history, genome collinearity and duplication of AhOMTs were studied in detail. Promoter cis-elements, protein-protein interactions, and micro-RNAs targeting AhOMTs were also predicted. We also comprehensively studied their expression in different tissues and under different stresses. We identified 116 OMT genes in the genome of cultivated peanut. Phylogenetically, AhOMTs were divided into three groups. Tandem and segmental duplication events played a role in the evolution of AhOMTs, and purifying selection pressure drove the duplication process. AhOMT promoters were enriched in several key cis-elements involved in growth and development, hormones, light, and defense-related activities. Micro-RNAs from 12 different families targeted 35 AhOMTs. GO enrichment analysis indicated that AhOMTs are highly enriched in transferase and catalytic activities, cellular metabolic and biosynthesis processes. Transcriptome datasets revealed that AhOMTs possessed varying expression levels in different tissues and under hormones, water, and temperature stress. Expression profiling based on qRT-PCR results also supported the transcriptome results. This study provides the theoretical basis for further work on the biological roles of AhOMT genes for developmental and stress responses.

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