An integrated multidisciplinary approach to resolve long-standing unidentified human skeletal remains in Cyprus.

The Committee on Missing Persons in Cyprus (CMP) is a bicommunal committee established in 1981, tasked to determine the fate of 2002 individuals who went missing during the intercommunal fighting of 1963-64 and the events of 1974. The CMP operates strictly within a humanitarian framework, using a multidisciplinary approach to conclude individual identifications of remains exhumed throughout the island, where all information obtained from different phases of the CMP Project is integrated and assessed in a comprehensive manner. By 2017, although over 1000 sets of remains were recovered and either identified or resolved by the CMP, 137 challenging cases remained unidentified at the CMP Anthropological Laboratory. To resolve these cases, different strategies were adopted where the investigatory component was enhanced through the implementation of new data mining approaches, and the genetic-related data were revised and updated through the adoption of new DNA technologies and the improvement of the Family Reference Samples Database. These new approaches resulted in a dramatic reduction of the number of unidentified cases (by over 70 %) as well as the timeframe required for future identifications. These approaches could serve as an example in other humanitarian contexts facing similar challenges as they can have a profound impact on the families of missing persons.

Second GHEP-ISFG exercise for DVI: "DNA-led" victims' identification in a simulated air crash.

The Spanish and Portuguese-Speaking Working Group of the International Society for Forensic Genetics (GHEP-ISFG) has organized a second collaborative exercise on a simulated case of Disaster Victim Identification (DVI), with the participation of eighteen laboratories. The exercise focused on the analysis of a simulated plane crash case of medium-size resulting in 66 victims with varying degrees of fragmentation of the bodies (with commingled remains). As an additional difficulty, this second exercise included 21 related victims belonging to 6 families among the 66 missings to be identified. A total number of 228 post-mortem samples were represented with aSTR and mtDNA profiles, with a proportion of partial aSTR profiles simulating charred remains. To perform the exercise, participants were provided with aSTR and mtDNA data of 51 reference pedigrees -some of which deficient-including 128 donors for identification purposes. The exercise consisted firstly in the comparison of the post-mortem genetic profiles in order to re-associate fragmented remains to the same individual and secondly in the identification of the re-associated remains by comparing aSTR and mtDNA profiles with reference pedigrees using pre-established thresholds to report a positive identification. Regarding the results of the post-mortem samples re-associations, only a small number of discrepancies among participants were detected, all of which were from just a few labs. However, in the identification process by kinship analysis with family references, there were more discrepancies in comparison to the correct results. The identification results of single victims yielded fewer problems than the identification of multiple related victims within the same family groups. Several reasons for the discrepant results were detected: a) the identity/non-identity hypotheses were sometimes wrongly expressed in the likelihood ratio calculations, b) some laboratories failed to use all family references to report the DNA match, c) In families with several related victims, some laboratories firstly identified some victims and then unnecessarily used their genetic information to identify the remaining victims within the family, d) some laboratories did not correctly use "prior odds" values for the Bayesian treatment of the episode for both post-mortem/post-mortem re-associations as well as the ante-mortem/post-mortem comparisons to evaluate the probability of identity. For some of the above reasons, certain laboratories failed to identify some victims. This simulated "DNA-led" identification exercise may help forensic genetic laboratories to gain experience and expertize for DVI or MPI in using genetic data and comparing their own results with the ones in this collaborative exercise.