1.
Phys Chem Chem Phys
; 20(9): 6159-6166, 2018 Feb 28.
Artículo
en Inglés
| MEDLINE
| ID: mdl-29457817
RESUMEN
Electron transfer kinetic parameters of site-specifically wired copper oxidase were investigated. The enzyme's orientation towards the electrode was controlled by incorporation of propargyl-l-lysine as a site-specific anchoring point. Herein, we demonstrate the importance of immobilization orientation and how it affects electron transfer efficiency and kinetics to each of the enzyme's two active sites.