Highly nonlinear yttrium-aluminosilicate optical fiber with a high intrinsic stimulated Brillouin scattering threshold.

Highly nonlinear (high-NA small-mode-area) optical fibers also possessing an intrinsically high stimulated Brillouin scattering threshold are described. More specifically, silica clad, yttrium-aluminosilicate core fibers are shown to exhibit an intrinsically low Brillouin gain coefficient between 0.125 and 0.139×10-11 m/W and a Brillouin gain linewidth of up to 500 MHz. Losses on the order of 0.7 dB/m were measured, resulting from impurities in the precursor materials. Nonlinear refractive index values are determined to be similar to that of silica, but significant measurement uncertainty is attributed to the need to estimate dispersion curves since their direct measurement could not be made. The interest for highly nonlinear optical fibers with a low intrinsic Brillouin gain coefficient is expected to continue, especially with the growing developments of narrow-linewidth high-energy laser systems.

Kerr nonlinear switching in a hybrid silica-silicon microspherical resonator.

A hybrid silicon-core, silica-clad microspherical resonator has been fabricated from the semiconductor core fiber platform. Linear and nonlinear characterization of the resonator properties have shown it to exhibit advantageous properties associated with both materials, with the low loss cladding supporting high quality (Q) factor whispering gallery modes which can be tuned through the nonlinear response of the crystalline core. By exploiting the large wavelength shift associated with the Kerr nonlinearity, we have demonstrated all-optical modulation of a weak probe on the timescale of the femtosecond pump pulse. This novel geometry offers a route to ultra-low loss, high-Q silica-based resonators with enhanced functionality.

Optical detection and modulation at 2µm-2.5µm in silicon.

Recently the 2µm wavelength region has emerged as an exciting prospect for the next generation of telecommunications. In this paper we experimentally characterise silicon based plasma dispersion effect optical modulation and defect based photodetection in the 2-2.5µm wavelength range. It is shown that the effectiveness of the plasma dispersion effect is dramatically increased in this wavelength window as compared to the traditional telecommunications wavelengths of 1.3µm and 1.55µm. Experimental results from the defect based photodetectors show that detection is achieved in the 2-2.5µm wavelength range, however the responsivity is reduced as the wavelength is increased away from 1.55µm.

Four-wave mixing and octave-spanning supercontinuum generation in a small core hydrogenated amorphous silicon fiber pumped in the mid-infrared.

An octave-spanning supercontinuum is generated in a hydrogenated amorphous silicon core fiber when pumped in the mid-infrared regime. The broadband wavelength conversion which extends from the edge of the telecommunications band into the mid-infrared (1.64-3.37 µm) is generated by four-wave mixing (FWM) and subsequent pulse break-up, facilitated by the high material nonlinear figure of merit and the anomalous dispersion of the relatively small 1.7 µm diameter core fiber. The FWM sidebands and corresponding supercontinuum can be tuned through the pump parameters, and show good agreement with the predicted phase-matching curves for the fiber.

Nonlinear transmission properties of hydrogenated amorphous silicon core fibers towards the mid-infrared regime.

The nonlinear transmission properties of hydrogenated amorphous silicon (a-Si:H) core fibers are characterized from the near-infrared up to the edge of the mid-infrared regime. The results show that this material exhibits linear losses on the order of a few dB/cm, or less, over the entire wavelength range, decreasing down to a value of 0.29 dB/cm at 2.7µm, and negligible nonlinear losses beyond the two-photon absorption (TPA) edge ~ 1.7µm. By measuring the dispersion of the nonlinear Kerr and TPA parameters we have found that the nonlinear figure of merit (FOM(NL)) increases dramatically over this region, with FOM(NL) > 20 around 2µm and above. This characterization demonstrates the potential for a-Si:H fibers and waveguides to find use in nonlinear applications extending beyond telecoms and into the mid-infrared regime.

Ultrafast wavelength conversion via cross-phase modulation in hydrogenated amorphous silicon optical fibers.

We present a characterization of the spectral modulation and wavelength shifting induced via cross-phase modulation (XPM) in a hydrogenated amorphous silicon (a-Si:H) core optical fiber. Pump-probe experiments using picosecond and femtosecond signal pulses are shown to be in good agreement with numerical simulations of the coupled nonlinear propagation equations. The large 10nm red-shifts obtained with the femtosecond probe pulses are attributed to the high Kerr nonlinearity of the a-Si:H material. Extinction ratios as high as 12 dB are measured for the conversion process at telecommunications wavelengths, indicating the potential for high-speed nonlinear optical control in a-Si:H fibers and waveguides.

All-optical modulation using two-photon absorption in silicon core optical fibers.

All-optical modulation based on degenerate and non-degenerate two-photon absorption (TPA) is demonstrated within a hydrogenated amorphous silicon core optical fiber. The nonlinear absorption strength is determined by comparing the results of pump-probe experiments with numerical simulations of the coupled propagation equations. Subpicosecond modulation is achieved with an extinction ratio of more than 4 dB at telecommunications wavelengths, indicating the potential for these fibers to find use in high speed signal processing applications.

High index contrast semiconductor ARROW and hybrid ARROW fibers.

We investigate the guidance properties of two photonic crystal fibers that have been fabricated by filling the holes of a silica template with hydrogenated amorphous silicon inclusions. The first is an all-solid fiber that guides light via an antiresonant reflecting optical waveguiding mechanism and the second is only partially filled so that it guides light by a hybrid of modified total internal reflection and antiresonant reflecting optical waveguiding. It will be shown that, by selectively filling the silica template to leave an unfilled internal ring of holes, the fiber's confinement loss can be reduced significantly. This novel fiber design in which the light guided in the silica core can be modified by the semiconductor cladding provides a route to integrating functional semiconductor fibers with existing silica fiber infrastructures.

Tapered silicon optical fibers.

The tapering of silicon optical fibers is demonstrated using a fusion splicer. The silicon fibers are fabricated using a high pressure chemical deposition technique to deposit an amorphous silicon core inside a silica capillary and the tapering is performed in a separate post-process. Optical and material characterization has revealed a smooth transition region leading to a uniform tapered waist that are both simultaneously crystallized to yield a solid polysilicon core. The strong mode confinement and low taper loss measured in the silicon fibers verifies this tapering approach for the fabrication of structures with nanoscale core dimensions.

Nonlinear transmission properties of hydrogenated amorphous silicon core optical fibers.

The nonlinear properties of a low loss hydrogenated amorphous silicon core fiber have been characterized for transmission of high power pulses at 1540 nm. Numerical modelling of the pulse propagation in the amorphous core material was used to establish the two-photon absorption, free-carrier absorption and the nonlinear refractive index, which were found to be larger than the values typical for crystalline silicon. Calculation of a nonlinear figure of merit demonstrates the potential for these hydrogenated amorphous silicon core fibers to be used in nonlinear silicon photonics applications.

Large mode area silicon microstructured fiber with robust dual mode guidance.

A silicon microstructured fiber has been designed and fabricated using a pure silica photonic bandgap guiding fiber as a 3D template for materials deposition. The resulting silicon fiber has a micron sized core but with a small core-cladding index contrast so that it only supports two guided modes. It will be shown that by using the microstructured template this fiber exhibits a number of similar guiding properties to the more traditional index guiding air-silica structures. The large mode areas and low optical losses measured for the silicon microstructured fiber demonstrate its potential to be integrated with existing fiber infrastructures.

Haptoglobin levels in serum of various strains of mice.

Only a single type of haptoglobin was found in the serum of 11 strains of mice, but there were wide variations between strains with respect to the amount of haptoglobin found. In the AKR and C3H strains, in which haptoglobin was low or absent, various agents stimulated production of high levels of haptoglobin. Serum haptoglobin rose in association with the development of leukemia in AKR mice, but remained low when C3H mice developed mammary tumors.

Estrogen-dependent increase in transfer RNA during differentiation of the chick oviduct.

Estrogenic hormones induce morphologic and biochemical differentiation in the oviduct of the immature chick. Concomitant with the hormone-stimulated tissue growth, there was an increase in 4S RNA, as judged by polyacrylamide-gel electrophoresis, and a corresponding increase in cellular transfer RNA activity, as measured by the amino acid acceptor capacity. This system may be suitable for studying the relation of hormones to transfer RNA in a differentiating tissue.

Conformational states of 18 S rRNA from HeLa cells detected by gel electrophoresis.

Gel electrophoretic analysis has been used to study some of the conformational properties of 18 S rRNA from HeLa cells. After isolation and treatment by heat or denaturants, the mobility of the RNA in 3% acrylamide/0.5% agarose composite gels was found to be altered; often there were two or more distinct components. Several of these forms could be interconverted by heating to 40 degrees C or by use of dimethyl sulfoxide. Although these comformers were easily distinguishable (by their electrophoretic properties), their melting profiles were very similar. Only a single electrophoretic form was detected when electrophoresis was performed at 40 degrees C. The conformational differences observed are probably derived by variations in base pairing and stacking that result when the parent form is cooled.

Hemoglobin binding by isolated polymeric proteins from human haptoglobin types 2-1 and 2-2. Some suggested polymer subunit compositions.

1. Some of the individual members of the polymeric series of proteins from human haptoglobin types 2-1 and 2-2 were isolated by gel electrophoresis. By reacting this purified material with less than an equivalent amount of hemoglobin and analyzing the result by electrophoresis, the number of haptoglobin-hemoglobin complexes could be clearly counted. For the haptoglobin 2-1 series, the number of complexes formed was n+1, where n is the serial order, in decreasing electrophoretic mobility, of the haptoglobin polymeric form used. For the haptoglobin 2-2 series, the number of complexes was n+2. 2. For the first three members of haptoglobin 2-1 series, the haptoglobin-hemoglobin composition of the complexes was estimated from scans of the unstained gels. The data indicated that this series consists of 2,3,4... alpha beta haptoglobin subunits, each of which can combine with an alpha beta subunit of hemoglobin.

Haptoglobin and albumin synthesis in isolated rat hepatocytes. Response to potential mediators of the acute-phase reaction.

Incorporation of [3H]leucine into haptoglobin and albumin by isolated rat hepatocyte suspensions was studied to assess the role of potential mediators of the acute-phase reaction in promoting haptoglobin synthesis. In vitro, in the presence of insulin, the addition of a hormone mixture containing hydrocortisone, glucagon, somatotropin, and triiodothyronine resulted in a 70% increase in leucine incorporation into haptoglobin relative to albumin at 48 h incubation. A variety of agents, selected because they are considered to play some part in the acute-phase reaction, were added to the medium, and similar measurements of leucine incorporation were made. The specific binding to hepatocytes by asialo- and asialo, agalacto-derivatives of haptoglobin or orosomucoid did not affect synthesis of haptoglobin or albumin. Epinephrine, prostaglandins E1 and E2, bacterial lipopolysaccharide, and sera containing active complement components also failed to stimulate relative haptoglobin synthesis. A partially purified preparation of human leukocytic pyrogen, however, caused a 70% increase in leucine incorporation into haptoglobin relative to albumin in the present of the hormone mixture, suggesting that this substance may affect acute-phase protein synthesis.

The induction of liver microsomal proteins by 3-methylcholanthrene in rats of different age, sex and strain.

The treatment of male and female adult and male and female immature rats with 3-methylcholanthrene results in increases in the intensity of two liver microsomal protein bands separated by sodium dodecyl sulphate-polyacrylacrylamide gel electrophoresis. The induced protein species have estimated molecular weights of about 55 000 and 52 500. The time course of the induction of these species has been followed in a semi-quantitative manner. In immature rats an additional band, corresponding to a protein species with a molecular weight of about 43 000, is also increased by 3- methylcholanthrene treatment. In male adult rats exclusively, treatment with 3-methylcholanthrene also results in a decrease in the intensity of a band corresponding to a protein species of about 51 000. The appearance of this band in the liver microsomal fraction of adult male rats occurs following sexual maturation and its presence represents a significant difference in the microsomal proteins between male and female rats; this protein is not detected in younger rats or in female rats at any age. These findings are discussed in terms of their possible relevance to the cytochrome P-450 content of the endoplasmic reticulum of rat hepatocytes.

Exact solutions of the generalized nonlinear Schrödinger equation with distributed coefficients.

A broad class of exact self-similar solutions to the nonlinear Schrödinger equation (NLSE) with distributed dispersion, nonlinearity, and gain or loss has been found describing both periodic and solitary waves. Appropriate solitary wave solutions applying to propagation in optical fibers and optical fiber amplifiers with these distributed parameters have also been studied in detail. These solutions exist for physically realistic dispersion and nonlinearity profiles. They correspond either to compressing or spreading solitary pulses which maintain a linear chirp or to chirped oscillatory solutions. The stability of these solutions has been confirmed by numerical simulations of the NLSE with perturbed initial conditions. These self-similar propagation regimes are expected to find practical application in both optical fiber amplifier systems and in fiber compressors.

Tunable continuous wave emission via phase-matched second harmonic generation in a ZnSe microcylindrical resonator.

Whispering gallery mode microresonators made from crystalline materials are of great interest for studies of low threshold nonlinear phenomena. Compared to amorphous materials, crystalline structures often exhibit desirable properties such as high indices of refraction, high nonlinearities, and large windows of transparency, making them ideal for use in frequency comb generation, microlasing and all-optical processing. In particular, crystalline materials can also possess a non-centrosymmetric structure which gives rise to the second order nonlinearity, necessary for three photon processes such as frequency doubling and parametric down-conversion. Here we report a novel route to fabricating crystalline zinc selenide microcylindrical resonators from our semiconductor fibre platform and demonstrate their use for tunable, low power continuous wave second harmonic generation. Visible red light is observed when pumped with a telecommunications band source by a process that is phase-matched between different higher order radial modes, possible due to the good spatial overlap between the pump and signal in the small volume resonator. By exploiting the geometrical flexibility offered by the fibre platform together with the ultra-wide 500-22000 nm transmission window of the ZnSe material, we expect these resonators to find use in applications ranging from spectroscopy to quantum information systems.

Bioassay for prolactin: densitometric analysis on polyacrylamide gels of milk protein production by mammary explants in vitro.

Mammary explants from mice in midpregnancy cultured in a synthetic medium containing insulin and hydrocortisone respond to the addition of prolactin by the synthesis of milk proteins. The secretory response in alveolar lumina has served as a histologic endpoint in a sensitive bioassay for prolactin developed by Kleinberg and Frantz (J Clin Invest 50: 1557, 1971). This report demonstrates that the quantitative densitometric analysis of stained milk proteins (caseins) made in response to prolactin is a useful modification of that bioassay. Electrophoretic analysis of the proteins extracted from the explants permits a quantitative estimate of casein content. The amount of casein present after 5 days of incubation was found to be a measure of the prolactin concentration in the medium. No radioactive isotopes are used. The use of electrophoretic analysis has practical advantages over the histologic scoring used earlier, and has approximately the same range of sensitivity.