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1.
Ann Hum Genet ; 80(3): 145-53, 2016 May.
Artículo en Inglés | MEDLINE | ID: mdl-26990426

RESUMEN

The hepatic damage caused by hepatitis C virus (HCV) infection is associated with the host immune response and viral regulatory factors. Catalase (CAT) and glutathione peroxidase 1 (GPX1) are antioxidant enzymes located in the peroxisomes and mitochondria, respectively, and are responsible for the control of intracellular hydrogen peroxide levels. Polymorphisms in CAT (C-262T) and GPX1 (Pro198Leu) are correlated with serum levels and enzyme activity. This study aimed to investigate the association of genetic polymorphisms of CAT C-262T (rs1001179) and GPX1 Pro198Leu (rs1050450) with different stages of liver fibrosis and development of hepatocellular carcinoma (HCC). This study included 445 patients with chronic hepatitis C, of whom 139 patients had mild fibrosis (F0-F1), 200 had moderate/severe fibrosis (F2-F4), and 106 had HCC. Genotyping of SNPs was performed by real-time PCR using TaqMan probes. The Pro/Pro genotype of GPX1 was significantly associated with fibrosis severity, HCC, Child Pugh score, and BCLC staging. Additionally, patients carrying both CT+TT genotypes in the CAT gene and the Pro/Pro genotype in the GPX1 gene had higher risk for developing moderate/severe fibrosis or HCC (p = 0.009, OR 2.40 and p = 0.002, OR 3.56, respectively). CAT and GPX1 polymorphisms may be implicated in the severity of liver fibrosis and HCC caused by HCV.


Asunto(s)
Catalasa/genética , Glutatión Peroxidasa/genética , Hepatitis C Crónica/genética , Polimorfismo de Nucleótido Simple , Adulto , Anciano , Alelos , Carcinoma Hepatocelular/genética , Femenino , Frecuencia de los Genes , Genotipo , Humanos , Cirrosis Hepática/genética , Neoplasias Hepáticas/genética , Masculino , Persona de Mediana Edad , Factores de Riesgo , Glutatión Peroxidasa GPX1
2.
BMC Infect Dis ; 13: 60, 2013 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-23374914

RESUMEN

BACKGROUND: Hepatitis C chronic liver disease is a major cause of liver transplant in developed countries. This article reports the first nationwide population-based survey conducted to estimate the seroprevalence of HCV antibodies and associated risk factors in the urban population of Brazil. METHODS: The cross sectional study was conducted in all Brazilian macro-regions from 2005 to 2009, as a stratified multistage cluster sample of 19,503 inhabitants aged between 10 and 69 years, representing individuals living in all 26 State capitals and the Federal District. Hepatitis C antibodies were detected by a third-generation enzyme immunoassay. Seropositive individuals were retested by Polymerase Chain Reaction and genotyped. Adjusted prevalence was estimated by macro-regions. Potential risk factors associated with HCV infection were assessed by calculating the crude and adjusted odds ratios, 95% confidence intervals (95% CI) and p values. Population attributable risk was estimated for multiple factors using a case-control approach. RESULTS: The overall weighted prevalence of hepatitis C antibodies was 1.38% (95% CI: 1.12%-1.64%). Prevalence of infection increased in older groups but was similar for both sexes. The multivariate model showed the following to be predictors of HCV infection: age, injected drug use (OR = 6.65), sniffed drug use (OR = 2.59), hospitalization (OR = 1.90), groups socially deprived by the lack of sewage disposal (OR = 2.53), and injection with glass syringe (OR = 1.52, with a borderline p value). The genotypes 1 (subtypes 1a, 1b), 2b and 3a were identified. The estimated population attributable risk for the ensemble of risk factors was 40%. Approximately 1.3 million individuals would be expected to be anti-HCV-positive in the country. CONCLUSIONS: The large estimated absolute numbers of infected individuals reveals the burden of the disease in the near future, giving rise to costs for the health care system and society at large. The known risk factors explain less than 50% of the infected cases, limiting the prevention strategies. Our findings regarding risk behaviors associated with HCV infection showed that there is still room for improving strategies for reducing transmission among drug users and nosocomial infection, as well as a need for specific prevention and control strategies targeting individuals living in poverty.


Asunto(s)
Hepacivirus , Hepatitis C/epidemiología , Adolescente , Adulto , Anciano , Brasil/epidemiología , Niño , Estudios Transversales , Femenino , Genotipo , Hepacivirus/genética , Hepacivirus/inmunología , Hepatitis C/historia , Anticuerpos contra la Hepatitis C/sangre , Anticuerpos contra la Hepatitis C/inmunología , Historia del Siglo XXI , Humanos , Masculino , Persona de Mediana Edad , Oportunidad Relativa , Prevalencia , Factores de Riesgo , Población Urbana , Adulto Joven
3.
Nutr J ; 10: 86, 2011 Aug 19.
Artículo en Inglés | MEDLINE | ID: mdl-21854603

RESUMEN

BACKGROUND/AIM: Hyperhomocysteinemia due to Methylenetetrahydrofolate Reductase (MTHFR) gene, in particular the C677T (Ala222Val) polymorphism were recently associated to steatosis and fibrosis. We analyzed the frequency of MTHFR gene in a cross-sectional study of patients affected by Chronic Hepatitis C (CHC) from Northeast of Brazil. METHOD: One hundred seven-four untreated patients with CHC were genotyped for the C677T MTHFR. Genomic DNA was extracted from peripheral blood cells and the C677T MTHFR polymorphism was identified by PCR-RFLP. The homocysteine (Hcy) levels were determined by chemiluminescence method. All patients were negative for markers of Wilson's disease, hemochromatosis and autoimmune diseases and have current and past daily alcohol intake less than 100 g/week. RESULTS: Among subjects infected with CHC genotype non-1 the frequency of MTHFR genotypes TT was 9.8% versus 4.4% genotype 1 (p = 0.01). Nevertheless, association was found between the MTHFR genotype TT × CT/CC polymorphism and the degree of steatosis and fibrosis in both hepatitis C genotype (p < 0.05). A significant difference was found on plasma Hcy levels in patients with steatosis regardless of HCV genotype (p = 0.03). CONCLUSION: Our results indicate that plasma Hcy levels is highly prevalent in subjects with chronic hepatits C with steatosis regardless of HCV genotype and vitamin deficiency. The presence of genotype TT of MTHFR C677T polymorphism was more common in CHC genotype non-1 infected patient regardless of histopathological classification and genotype TT+CT frequencies were significant in the presence of fibrosis grade 1+2 and of steatosis in CHC infected patients from the northeast of Brazil regardless of HCV genotype. The genetic susceptibility of MTHFR C677T polymorphism should be confirmed in a large population.


Asunto(s)
Hepatitis C Crónica/genética , Homocisteína/sangre , Metilenotetrahidrofolato Reductasa (NADPH2)/genética , Brasil/epidemiología , Estudios Transversales , Femenino , Genotipo , Hepatitis C Crónica/epidemiología , Hepatitis C Crónica/patología , Humanos , Hiperhomocisteinemia/genética , Hiperhomocisteinemia/patología , Masculino , Persona de Mediana Edad , Polimorfismo Genético
4.
Artículo en Inglés | MEDLINE | ID: mdl-33909848

RESUMEN

Hepatitis A virus (HAV) infection has been considered one of the leading causes of acute hepatitis. The aim of the present study was to estimate the prevalence of HAV among children and adolescents in a population-based study in the capitals of the States of the North, Southeast and South of Brazil and identify predictive factors for the infection. A multi-stage sampling was used to select subjects aged between 5-9 and 10-19 years. Individual and household levels aside from the level of variables in the areas were collected. The outcome was the total IgG antibodies to HAV levels detected using a commercial Enzyme Immuno Assay (EIA). The associations between HAV and the independent variables were assessed using the odds ratio. A multilevel analysis was performed by GLLAMM using the Stata software. The prevalence of HAV infection in the 5-9 and 10-19 age groups was 28.7% and 67.5%, respectively for the North, 20.6% and 37.7%, for the Southeast and 18.9% and 34.5% for the South Region. The prevalence of HAV increased according to age in all sites. Variables related to education at the individual level (North and South), family and area level (South and Southeast) and family income level (Southeast and South) were independently associated with HAV infection. This emphasizes the need for individualized strategies to prevent the infection.


Asunto(s)
Hepatitis A , Adolescente , Brasil/epidemiología , Niño , Hepatitis A/diagnóstico , Hepatitis A/epidemiología , Anticuerpos de Hepatitis A , Humanos , Prevalencia , Factores de Riesgo , Estudios Seroepidemiológicos
5.
Clinics (Sao Paulo) ; 76: e2881, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34644732

RESUMEN

OBJECTIVE: The folate pathway is involved in hepatic carcinogenesis and angiogenesis. Polymorphisms in genes related to such processes, including methylene tetrahydrofolate reductase (MTHFR) and vascular endothelial growth factor (VEGF)] may play an important role in the development of hepatocellular carcinoma (HCC). The objective of this study was to evaluate MTHFR and VEGF polymorphisms in Brazilian patients with hepatitis C virus (HCV)-related HCC. METHODS: A total of 119 patients diagnosed with confirmed HCC and HCV were included in the study. SNP genotyping assays were performed using real-time PCR. VEGFA (rs2010963, rs3025039, and rs833061) and MTHFRC677T (rs1801133, rs1801131) polymorphisms were evaluated. RESULTS: The C alleles of MTHFR (rs1801131) and VEGF (rs2010963) were associated with protection against the development of multinodular HCC, while the T allele of MTHFR (rs1801133) was associated with a higher risk of multinodular presentation [p=0.04 OR 1.835 CI (1.022-3.297)]. Multivariate analysis revealed that the GG/GC genotypes of VEGF rs2010963 were independently associated with multinodular tumors at diagnosis (p=0.013; OR 4.78 CI (1.38-16.67)]. CONCLUSION: Our results suggest that these polymorphisms may increase the risk of rapid tumor progression in patients with HCV infection. This subgroup of patients with HCC and who present polymorphism is more likely to be diagnosed with multinodular disease and not be amenable to receiving curative treatments. These data must be validated in larger cohorts, and the screening intervals can be customized based on genetic history.


Asunto(s)
Carcinoma Hepatocelular , Hepatitis C , Neoplasias Hepáticas , Carcinoma Hepatocelular/genética , Estudios de Casos y Controles , Predisposición Genética a la Enfermedad , Genotipo , Hepacivirus , Humanos , Neoplasias Hepáticas/genética , Metilenotetrahidrofolato Reductasa (NADPH2)/genética , Polimorfismo de Nucleótido Simple , Factor A de Crecimiento Endotelial Vascular/genética
6.
J Immunol Methods ; 460: 101-106, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-30056939

RESUMEN

The aim of this study was to develop an assay to analyze the serum profile of Mannose-binding lectin (MBL) through a simple and "in-house" method (called "dot-N-man"). Furthermore, the study attempted to associate molecular masses of MBL to the profile of MBL gene polymorphisms in patients with hepatitis C. Heterogeneity in molecular masses of MBL is due to the impairment of oligomers formation, which is linked to genetic polymorphisms in the MBL gene. Individuals with AA genotype (wild-type) produce high-molecular-mass proteins, whereas AO and OO individuals produce intermediate and low-molecular-mass proteins, respectively. Sera of thirty patients carrying the hepatitis C virus (HCV) were investigated using MBL binding assay with mannan-coated nitrocellulose (dot-N-man). Purified MBL was evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Dot-N-Man assay yielded MBL with molecular masses ranging between 55 and 320 kDa, comparable to low and high molecular mass forms of MBL. Nonreducing SDS-PAGE showed high molecular mass bands in all AA individuals while bands of 270 and 205 kDa were observed in sera for a number of patients with AO and OO genotypes, respectively. Immunoblotting confirmed the MBL samples obtained from the dot-N-man. These results provide new insights to understand the MBL molecular forms profile in patients infected with HCV- which could be useful in future investigations on the influence of the MBL structure/genotype on both the progression of infection and the response to hepatitis C therapy.


Asunto(s)
Hepacivirus/inmunología , Hepatitis C , Immunoblotting/métodos , Lectina de Unión a Manosa , Polimorfismo Genético , Colodión/química , Femenino , Hepatitis C/genética , Hepatitis C/inmunología , Humanos , Masculino , Mananos/química , Lectina de Unión a Manosa/genética , Lectina de Unión a Manosa/inmunología
7.
Clinics ; Clinics;76: e2881, 2021. tab
Artículo en Inglés | LILACS | ID: biblio-1339702

RESUMEN

OBJECTIVE: The folate pathway is involved in hepatic carcinogenesis and angiogenesis. Polymorphisms in genes related to such processes, including methylene tetrahydrofolate reductase (MTHFR) and vascular endothelial growth factor (VEGF)] may play an important role in the development of hepatocellular carcinoma (HCC). The objective of this study was to evaluate MTHFR and VEGF polymorphisms in Brazilian patients with hepatitis C virus (HCV)-related HCC. METHODS: A total of 119 patients diagnosed with confirmed HCC and HCV were included in the study. SNP genotyping assays were performed using real-time PCR. VEGFA (rs2010963, rs3025039, and rs833061) and MTHFRC677T (rs1801133, rs1801131) polymorphisms were evaluated. RESULTS: The C alleles of MTHFR (rs1801131) and VEGF (rs2010963) were associated with protection against the development of multinodular HCC, while the T allele of MTHFR (rs1801133) was associated with a higher risk of multinodular presentation [p=0.04 OR 1.835 CI (1.022-3.297)]. Multivariate analysis revealed that the GG/GC genotypes of VEGF rs2010963 were independently associated with multinodular tumors at diagnosis (p=0.013; OR 4.78 CI (1.38-16.67)]. CONCLUSION: Our results suggest that these polymorphisms may increase the risk of rapid tumor progression in patients with HCV infection. This subgroup of patients with HCC and who present polymorphism is more likely to be diagnosed with multinodular disease and not be amenable to receiving curative treatments. These data must be validated in larger cohorts, and the screening intervals can be customized based on genetic history.


Asunto(s)
Humanos , Hepatitis C , Carcinoma Hepatocelular/genética , Neoplasias Hepáticas/genética , Estudios de Casos y Controles , Hepacivirus , Predisposición Genética a la Enfermedad , Polimorfismo de Nucleótido Simple , Metilenotetrahidrofolato Reductasa (NADPH2)/genética , Factor A de Crecimiento Endotelial Vascular/genética , Genotipo
8.
Am J Trop Med Hyg ; 93(6): 1341-1348, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26503280

RESUMEN

A population-based hepatitis survey was carried out to estimate the prevalence of hepatitis B virus (HBV) infection and its predictive factors for the state capitals from the north, south, and southeast regions of Brazil. A multistage cluster sampling was used to select, successively, census tracts, blocks, households, and residents in the age group 10-69 years in each state capital. The prevalence of hepatitis B surface antigen (HBsAg) was lower than 1% in the north, southeast, and south regions. Socioeconomic condition was associated with HBV infection in north and south regions. Variables related to the blood route transmission were associated with HBV infection only in the south whereas those related to sexual behavior were associated with HBV infection in the north and south regions. Drug use was associated in all regions, but the type of drug differed. The findings presented herein highlight the diversity of the potential transmission routes for hepatitis B transmission in Brazil. In one hand, it reinforces the importance of national control strategies of large impact already in course (immunization of infants, adolescents, and adults up to 49 years of age and blood supply screening). On the other hand, it shows that there is still room for further control measures targeted to different groups within each region.


Asunto(s)
Vacunas contra Hepatitis B/uso terapéutico , Hepatitis B/epidemiología , Adolescente , Adulto , Factores de Edad , Anciano , Brasil/epidemiología , Niño , Femenino , Encuestas Epidemiológicas , Hepatitis B/etiología , Hepatitis B/prevención & control , Hepatitis B/transmisión , Antígenos de Superficie de la Hepatitis B/sangre , Humanos , Masculino , Persona de Mediana Edad , Prevalencia , Factores de Riesgo , Enfermedades Virales de Transmisión Sexual/epidemiología , Factores Socioeconómicos , Abuso de Sustancias por Vía Intravenosa/complicaciones , Adulto Joven
9.
Rev Inst Med Trop Sao Paulo ; 45(3): 137-40, 2003.
Artículo en Inglés | MEDLINE | ID: mdl-12870062

RESUMEN

In order to assess the potential risk of anti-HBc-positive blood donors for post-transfusional hepatitis and to investigate whether other HBV serological markers are capable of identifying the presence of the virus, 1000 first-time blood donors were enrolled between June and July 1997. These donors were screened using routine Brazilian blood center tests (HIV 1 and 2, HTLV 1 and 2, Chagas disease, Syphilis, HCV, HBsAg, anti-HBc and ALT ). The 120 (12%) found to be anti-HBc-positive underwent further tests: HBe, anti-HBe, anti-HBs and HBV-DNA by PCR. Ten cases were HBsAg positive and all were HBV-DNA positive by PCR. Three HBsAg-negative donors were HBV-DNA-positive. Two HBV-DNA-positive donors were also anti-HBs-positive. All the HBV-positive donors had at least one HBV marker other than anti-HBc. Anti-HBc is an important cause of blood rejection. Testing for HBsAg alone is not fully protective and anti-HBc remains necessary as a screening test. The presence of anti-HBs is not always indicative of absence of the virus. The addition of other HBV serological markers could represent an alternative in predicting the presence of the virus when compared with PCR. It is recommended that other studies should be carried out to confirm this finding.


Asunto(s)
Donantes de Sangre , ADN Viral/sangre , Anticuerpos contra la Hepatitis B/sangre , Virus de la Hepatitis B/aislamiento & purificación , Biomarcadores/sangre , Estudios Transversales , ADN Viral/genética , Ensayo de Inmunoadsorción Enzimática , Femenino , Hepatitis B/prevención & control , Antígenos del Núcleo de la Hepatitis B/sangre , Antígenos de Superficie de la Hepatitis B/sangre , Virus de la Hepatitis B/genética , Anticuerpos contra la Hepatitis C/sangre , Humanos , Mediciones Luminiscentes , Masculino , Reacción en Cadena de la Polimerasa
10.
Viral Immunol ; 23(4): 449-53, 2010 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-20712490

RESUMEN

Patients with hepatitis B virus (HBV) infection may develop severe chronic liver disease. Carriers of HBV have an increased risk of developing cirrhosis, hepatic decompensation, and hepatocellular carcinoma. Worldwide an estimated 350 million people are infected with HBV, and 15-40% will develop serious sequelae in their lifetime. In our study we investigated the association of single nucleotide polymorphisms (SNPs) in the first exon and promoter region of the mannose-binding lectin gene 2 (MBL2) situated on chromosome 10, with susceptibility to HBV infection. One-hundred and two patients infected with HBV were included in this study, and 232 uninfected individuals were used as healthy controls. Genotyping of the first exon (alleles A/O) was performed using a melting temperature assay. Genotyping of the promoter region (-550 H/L; -221 Y/X) was performed using the Taqman PCR technique. In the HBV-infected group we found a significantly increased frequency of haplotypes associated with low serum MBL. Our findings may indicate that MBL has a protective role against HBV infection in the studied population.


Asunto(s)
Predisposición Genética a la Enfermedad , Hepatitis B/genética , Lectina de Unión a Manosa/genética , Polimorfismo de Nucleótido Simple , Alelos , Brasil , Exones/genética , Femenino , Frecuencia de los Genes , Haplotipos , Hepatitis B/inmunología , Humanos , Inmunidad Innata , Masculino , Persona de Mediana Edad , Regiones Promotoras Genéticas/genética
11.
Am J Trop Med Hyg ; 81(2): 240-7, 2009 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-19635877

RESUMEN

This multicentric population-based study in Brazil is the first national effort to estimate the prevalence of hepatitis B (HBV) and risk factors in the capital cities of the Northeast, Central-West, and Federal Districts (2004-2005). Random multistage cluster sampling was used to select persons 13-69 years of age. Markers for HBV were tested by enzyme-linked immunosorbent assay. The HBV genotypes were determined by sequencing hepatitis B surface antigen (HBsAg). Multivariate analyses and simple catalytic model were performed. Overall, 7,881 persons were included; < 70% were not vaccinated. Positivity for HBsAg was less than 1% among non-vaccinated persons and genotypes A, D, and F co-circulated. The incidence of infection increased with age with similar force of infection in all regions. Males and persons having initiated sexual activity were associated with HBV infection in the two settings; healthcare jobs and prior hospitalization were risk factors in the Federal District. Our survey classified these regions as areas with HBV endemicity and highlighted the risk factors differences among the settings.


Asunto(s)
Hepatitis B/epidemiología , Adolescente , Adulto , Anciano , Brasil/epidemiología , Estudios Transversales , Femenino , Anticuerpos contra la Hepatitis B/sangre , Humanos , Masculino , Persona de Mediana Edad , Oportunidad Relativa , Prevalencia , Factores de Riesgo , Caracteres Sexuales , Factores Socioeconómicos , Adulto Joven
12.
J Med Virol ; 67(1): 27-32, 2002 May.
Artículo en Inglés | MEDLINE | ID: mdl-11920814

RESUMEN

The distributions of the different genotypes of the hepatitis C virus (HCV) and GBV-C virus (GBV-C/HGV) vary geographically and information worldwide is still incomplete. In particular, there are few data on the distribution of genotypes (and their relationship to the severity of liver disease) in South America. Findings are described in 114 consecutive patients from Northeast Brazil (median age 52 years, range 18-72 years) who had abnormal levels of serum aminotransferases and seropositivity for HCV RNA. The patients were recruited from an outpatient clinic between November 1997 and April 1998. Quantitative HCV RNA and GBV-C/HGV RNA estimations were carried out by double-nested polymerase chain reaction (PCR) using primers from the 5'-untranslated regions (UTRs) of the genomes. HCV genotypes were determined by restriction fragment length polymorphism (RFLP) analysis with 5'-UTR primers and by PCR with type-specific 5'-UTR primers. GBV-C/HGV-RNA genotypes were determined by RFLP with specific 5'-UTR primers and phylogenetic trees were constructed using the Neighbour-Joining and Drawtree programs. Histological features were graded and staged according to international criteria. Of the 114 patients, 35 (30.7%) patients had cirrhosis and 22 (27.8%) had mild, 51 (64.6%) had moderate, and 6 (7.6%) had severe chronic hepatitis. Median HCV viral load was 10(6) genome equivalents per millilitre (range 10(4)-10(9)/ml). Frequencies of genotypes were 5.3% type 1a, 44.7% type 1b, 3.5% type 2, 41.2% type 3, and 5.3% mixed types. GBV-C/HGV-RNA was detected in the sera of 12 (10.5%) patients and was distributed among three phylogenetic groups. There were no significant differences between patients with the predominant HCV genotypes (1b and 3) with respect to gender, age group, viral load, severity of liver disease, or coinfection with GBV-C/HGV.


Asunto(s)
Infecciones por Flaviviridae/complicaciones , Virus GB-C , Hepacivirus/genética , Hepatitis C Crónica/fisiopatología , Hepatitis Viral Humana/complicaciones , Carga Viral , Adolescente , Adulto , Anciano , Brasil/epidemiología , ADN Viral/análisis , Femenino , Infecciones por Flaviviridae/fisiopatología , Genotipo , Hepacivirus/clasificación , Hepatitis C Crónica/complicaciones , Hepatitis C Crónica/epidemiología , Hepatitis C Crónica/virología , Hepatitis Viral Humana/fisiopatología , Humanos , Masculino , Persona de Mediana Edad , Filogenia , Polimorfismo de Longitud del Fragmento de Restricción , Vigilancia de la Población , Prevalencia
13.
Rev. Inst. Med. Trop. Säo Paulo ; Rev. Inst. Med. Trop. Säo Paulo;45(3): 137-140, May-Jun. 2003.
Artículo en Inglés | LILACS | ID: lil-342165

RESUMEN

In order to assess the potential risk of anti-HBc-positive blood donors for post-transfusional hepatitis and to investigate whether other HBV serological markers are capable of identifying the presence of the virus, 1000 first-time blood donors were enrolled between June and July 1997. These donors were screened using routine Brazilian blood center tests (HIV 1 and 2, HTLV 1 and 2, Chagas disease, Syphilis, HCV, HBsAg, anti-HBc and ALT ). The 120 (12 percent) found to be anti-HBc-positive underwent further tests: HBe, anti-HBe, anti-HBs and HBV-DNA by PCR. Ten cases were HBsAg positive and all were HBV-DNA positive by PCR. Three HBsAg-negative donors were HBV-DNA-positive. Two HBV-DNA-positive donors were also anti-HBs-positive. All the HBV-positive donors had at least one HBV marker other than anti-HBc. Anti-HBc is an important cause of blood rejection. Testing for HBsAg alone is not fully protective and anti-HBc remains necessary as a screening test. The presence of anti-HBs is not always indicative of absence of the virus. The addition of other HBV serological markers could represent an alternative in predicting the presence of the virus when compared with PCR. It is recommended that other studies should be carried out to confirm this finding


Asunto(s)
Humanos , Biomarcadores , Donantes de Sangre , ADN Viral , Doping en los Deportes , Anticuerpos contra la Hepatitis B , Virus de la Hepatitis B , Estudios Transversales , ADN Viral , Ensayo de Inmunoadsorción Enzimática , Antígenos del Núcleo de la Hepatitis B , Antígenos de Superficie de la Hepatitis B , Virus de la Hepatitis B , Anticuerpos contra la Hepatitis C , Reacción en Cadena de la Polimerasa
14.
GED gastroenterol. endosc. dig ; GED gastroenterol. endosc. dig;20(3): 71-77, maio-jun. 2001. tab, graf
Artículo en Portugués | LILACS | ID: lil-303450

RESUMEN

Foram estudados 234 pacientes com essquistossomose mansoni, sendo 44 com a forma hepatintestinal (EHI), 143 com a forma hepatesplenica compensada (EHEC) e 47 com a forma descompensada (EHED), além de 50 individuos controles. Dos 234 pacientes, 133 (57por cento) tinham pelo menos um marcador sorológico dis HBV e hcv. A frequencia do HBsAg foi significativamente maior (21por cento vs. 6por cento vs. 2por cento) nos com EHED do que nos com EHEC ou nos com EHI (p<0,001. Dos 19 (8por cento) AgHBs positivos,14 (74por cento) foram ant-HBe (um com EHI, sete com EHEC e seis com EHED),incluindo sete (37 por cento) HBV-DNA positivos. Em relaçao ao HCV, 47 (20por cento) dos 234 pacientes foram anti-HCV poistivos, apresentaram também HCV-RNA no soro, sendo11 (69,8 por cento) dos 16 com EHEC,17 (56,7 por cento) dos com EHED e um com EHI. O HCV_DNA foi detectado tambem em cinco pacientes anti-HCV negativos com EHED, totalizando 22 pacientes cm infeccao pelo HCV. Nos 108 pacientes com EHE ( 13 HBsAg positivos, 25 anti-HCV positivos para ambos os marcadores submetidos a esplenectomia) em que foi realizada biópsia hepatica dos com EHEC apresentava hepatite cronica ativa. Os achados deste estudo sugerem que a infecçao concomitante pelos virus das hepatites B e C é importante fator contribuinte para a gravidade da doença hepatica na esquistossomose mansoni


Asunto(s)
Humanos , Masculino , Femenino , Adulto , Hepatitis B , Antígenos del Núcleo de la Hepatitis B , Hepatitis C , Anticuerpos contra la Hepatitis C , Esquistosomiasis mansoni , Biopsia , Pruebas Serológicas
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