Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
1.
Clin Lab ; 62(12): 2423-2428, 2016 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-28164554

RESUMEN

BACKGROUND: We evaluated a modified Roche NH3L method developed by our group that significantly reduced the error flag "> ABS (> Absorbance)" on the COBAS 6000 (c501 module) automated platform. METHODS: Our study was finalized to validate the NH3L open method on COBAS 6000 (c501 module) with imprecision and correlation tests. In addition, the NH3L open method was evaluated for determination of lower limit of blank (LoB), lower limit of detection (LoD), and accuracy. RESULTS: The imprecision test showed good results with CV for all samples tested < 3 and < 5 for within-run and between-run assays. Correlation tests of NH3L classic and NH3L open method showed good correlation with R square = 0.95. "> ABS" obtained with the NH3L open were only 2% compared to NH3L classic method. CONCLUSIONS: Our study shows that the NH3L open method is reproducible and stable, providing values which correlate with those obtained by the traditional method. The ability to reduce the alarm > ABS by more than 95% thanks to lower background absorbance values makes this method reliable, avoiding re-testing or the need for sample dilutions.


Asunto(s)
Amoníaco/sangre , Análisis Químico de la Sangre/instrumentación , Ensayos Analíticos de Alto Rendimiento/instrumentación , Automatización de Laboratorios , Biomarcadores/sangre , Diseño de Equipo , Humanos , Límite de Detección , Miniaturización , Valor Predictivo de las Pruebas , Reproducibilidad de los Resultados
3.
Clin Biochem ; 41(10-11): 914-6, 2008 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-18439912

RESUMEN

OBJECTIVE: To identify the real number of hyperhomocysteinemic Alzheimer's patients who may benefit from homocysteine-lowering therapy. METHODS: Basal and post-methionine load homocysteine levels were assessed by rp-HPLC system. RESULTS: PML test revealed twice as many hyperhomocysteinemic AD subjects with respect to the fasting analysis. CONCLUSION: PML test resulted useful in detecting higher number of hyperhomocysteinemic AD patients who may have the chance of an early folate treatment.


Asunto(s)
Enfermedad de Alzheimer/complicaciones , Hiperhomocisteinemia/diagnóstico , Metionina/administración & dosificación , Anciano , Anciano de 80 o más Años , Enfermedad de Alzheimer/sangre , Cromatografía Líquida de Alta Presión/métodos , Ácido Fólico/sangre , Ácido Fólico/uso terapéutico , Homocisteína/sangre , Humanos , Hiperhomocisteinemia/sangre , Hiperhomocisteinemia/tratamiento farmacológico , Hiperhomocisteinemia/etiología , Metionina/sangre , Sensibilidad y Especificidad
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA