Histopathologic aspects of the so-called chronic hyperplastic candidiasis: An analysis of 36 cases.

BACKGROUND: The present study aimed to analyze the histopathologic aspects of cases diagnosed as chronic hyperplastic candidiasis (CHC) in an oral pathology service, pointing out the most important features found. METHODS: All cases histopathologically diagnosed as CHC between 2002 and 2018 were retrieved from the files of the service. Data of the patients and the lesions were collected. Histopathological analysis was performed to evaluate the microscopic characteristics and the amount of Candida present. RESULTS: Thirty-six cases of CHC were retrieved. Women were more affected and there was a predilection for Whites. Most lesions appeared as a nodule or a white plaque, asymptomatic, on the tongue or buccal mucosa. Histopathologically, statistical significance was noted for the presence of epithelial hyperplasia, exocytosis, and mononuclear inflammatory infiltrate. Interestingly, only the association between dysplasia and the amount of fungus was noted. CONCLUSIONS: CHC appears to be a distinct entity presenting typical histopathologic aspects. CHC might even show epithelial dysplasia. In those cases, the presence of a large amount of Candida together with other histopathological features should lead to the diagnosis of CHC and subsequent treatment. However, close follow up is important.

Bilateral lipomatous hamartoma of the tongue: A case report in a child with oral-facial-digital syndrome type VI.

A hamartoma is a benign proliferation of typical mature cells specific to a particular anatomical site. In the oral cavity, they may occur as isolated cases or be associated with genetic syndromes. Oral-facial-digital syndrome type VI is a rare genetic disorder with an estimated incidence of one in 50,000-250,000 newborns. Here, we report a case of a 2-year-old boy diagnosed with oral-facial-digital syndrome type VI who was referred for evaluation of a bilateral and normochromic to slightly pinkish nodule on the lateral surface of the tongue. Clinically, the child presented hypotonia, low visual acuity, absence of oculocephalic reflex, delay in neuropsychomotor development, and polydactyly in the feet. Excisional biopsies of both sides of the tongue were performed using a 1.5 W high-power diode laser (wavelength of 980 nm), and histopathological analysis revealed abundant mature adipocytes predominantly arranged in lobules that mainly surrounded the minor salivary gland parenchyma. The surgical sites healed with no complications and the patient remains under follow-up for 10 months. Due to the limited literature on this syndrome and the frequent presence of tongue hamartomas in children, dentists need to be familiar with them.

Micropropagação de Croton antisyphiliticus Mart. / Micropropagation of Croton antisyphiliticus Mart.

Croton antisyphiliticus Mart. ex M. Arg., conhecido popularmente como pé-de-perdiz, é uma planta medicinal nativa do Cerrado, cuja raiz é utilizada na forma de decoctos para combater infecções do aparelho reprodutor masculino e feminino. A coleta da planta é realizada de forma extrativista e não há trabalhos a respeito da sua conservação. O objeto do trabalho foi estabelecer um protocolo de micropropagação de Croton antisyphiliticus. Foram avaliados o efeito de diferentes concentrações das citocininas, o tamanho do recipiente para o cultivo in vitro, o co-cultivo e a posição da gema em relação ao desenvolvimento dos explantes, bem como a influência do AIB no enraizamento in vitro e a aclimatização dos explantes. O melhor desenvolvimento in vitro foi obtido com explantes do tipo gema apical, co-cultivadas em meio MS suplementado com BAP (1µM), em frasco tipo pote. O enraizamento foi obtido em meio MS sem regulador vegetal e a aclimatização pode ser realizada em solo do cerrado com brotações sem a presença de raízes desenvolvidas in vitro.

Croton antisyphiliticus Mart. ex M. Arg., popularly known as "pé-de-perdiz", is a native medicinal plant of Cerrado, whose root is used in the form of decoctions against infections of the male and female reproductive. The collection of the plant is carried through of extractives form and there are no studies regarding its conservation. The aim of this study was to establish a micropropagation protocol of C. antisyphiliticus. The effect of different concentrations of cytokinins, the size of the container for the in vitro culture, co-cultivation and bud position in relation to the development of the explants, as well as the influence of AIB on in vitro rooting and acclimatization of explants were evaluated. The best development in vitro was obtained with apical explants co-cultivated on MS medium supplemented with BAP (1µM) in bottle type pot. Rooting was achieved on MS medium without growth regulators and acclimatization can be performed in Cerrado soil without the presence of shoots developed roots in vitro.