1.
Antonie Van Leeuwenhoek
; 46(4): 353-62, 1980.
Artículo
en Inglés
| MEDLINE
| ID: mdl-6255867
RESUMEN
Populations of a Escherichia coli K 12 strain, containing the vector plasmid pBR 322, were grown in chemostat culture under glucose- and phosphate-limited conditions. Resistance to tetracycline and ampicillin were lost after prolonged cultivation, resulting in the production of apparent plasmid-free populations which were more competitive than the original population. This competitiveness between plasmid-free and plasmid-containing populations was greatest in environments where the nutrient restriction was severe. Also during sequential subcultivation in batch cultures loss of plasmid was observed.