RESUMEN
Alt a 1 has been recognized as the most important allergen produced by the Pleosporaceae family and is a risk factor for asthma development and/or exacerbation. The aim of this study was to develop a detection tool that is highly specific for species that produced airborne Alt a 1. Based on the highly conserved internal nucleotide region of the several Alt a 1 sequences that are available in GenBank, a pair of primers (Alta1CF/Alta1CR) was designed. A set of primers used by other authors for the production of recombinant Alt a 1 (A21F/A21R) was also tested. The molecular analyses were based on the polymerase chain reaction (PCR) amplification and sequencing of the cDNA that was obtained from thirteen common fungal species. The PCR system that utilized Alta1CF/Alta1CR was highly specific, sensitive, and was able to detect an amplicon of approximately 180 bp from Alt a 1 and Alt a 1-like encoding genes from A. alternata, A. tenuissima, A. infectoria, U. botrytis, and S. botryosum. In contrast, the A21F/A21R primers were specific for the very close taxonomically related species A. alternata and A. tenuissima. Thus, this rapid, sensitive, and specific detection tool can be used to assess Alt a 1 exposure levels and to inform the implementation of the appropriate public health measures.
Asunto(s)
Antígenos Fúngicos/genética , Hongos/genética , Técnicas de Tipificación Micológica/métodos , Reacción en Cadena de la Polimerasa/métodos , ADN de Hongos/análisis , ADN de Hongos/genética , Monitoreo del Ambiente , Hongos/clasificación , Alineación de Secuencia , Análisis de Secuencia de ADNRESUMEN
BACKGROUND: Crude latex extracts are commonly used in skin prick tests (SPT) for the diagnosis of natural rubber latex (NRL) allergy. Nevertheless, variations in protein and allergen composition between latex extracts from different manufacturers can hamper a correct diagnosis. OBJECTIVES: To analyze the heterogeneity of proteins and allergens in latex extracts from 7 different manufacturers and to assess its relevance in the diagnosis of latex allergy. METHODS: Seven latex SPT extracts were analyzed for protein content using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The 4 major allergens Hev b 1, Hev b 3, Hev b 5, and Hev b 6.02 were also quantified using enzyme immunoassay. All commercial extracts were tested for their in vitro allergenic capacity using microarray inhibition assays and for their ability to induce biological reactivity in latex-allergic patients undergoing SPT. RESULTS: The protein content of the extracts varied widely from 8.0 microg/mL to 526.5 microg/mL. SDS-PAGE revealed broad differences in protein profiles between the extracts. Marked variability in the contents of all 4 major allergens was observed, and Hev b 3 and Hev b 5 were undetectable in some extracts. Microarray inhibition assays and SPT demonstrated relevant differences in allergenic capacity between the extracts. CONCLUSIONS: The marked heterogeneity in protein and allergen content of latex extracts from different manufacturers could explain the broad spectrum of SPT results recorded. Our findings suggest that the extracts used for the diagnosis of latex allergy should be improved and standardized.
Asunto(s)
Hipersensibilidad al Látex/diagnóstico , Pruebas Cutáneas , Adolescente , Adulto , Femenino , Humanos , Técnicas para Inmunoenzimas , Látex/análisis , Látex/inmunología , Masculino , Persona de Mediana Edad , Proteínas de Plantas/análisisRESUMEN
BACKGROUND: The diagnosis of anaphylactic reactions due to opiates during anaesthesia can be difficult, since in most cases various drugs may have been administered. Detection of specific IgE to poppy seed might be a marker for sensitisation to opiates in allergic people and heroin-abusers. This study assessed the clinical value of morphine, pholcodine and poppy seed skin-prick and IgE determination in people suffering hypersensitivity reactions during anaesthesia or analgesia and drug-abusers with allergic symptoms. METHODS: We selected heroin abusers and patients who suffered severe reactions during anaesthesia and analgesia from a database of 23,873 patients. The diagnostic yield (sensitivity, specificity and predictive value) of prick and IgE tests in determining opiate allergy was analysed. RESULTS: Overall, 149 patients and 200 controls, mean age 32.9 ± 14.7 years, were included. All patients with positive prick to opiates showed positive prick and IgE to poppy seeds, but not to morphine or pholcodine IgE. Among drug-abusers, 13/42 patients (31%) presented opium hypersensitivity confirmed by challenge tests. Among non-drug abusers, sensitisation to opiates was higher in people allergic to tobacco (25%), P<.001. Prick tests and IgE against poppy seed had a good sensitivity (95.6% and 82.6%, respectively) and specificity (98.5% and 100%, respectively) in the diagnosis of opiate allergy. CONCLUSIONS: Opiates may be significant allergens. Drug-abusers and people sensitised to tobacco are at risk. Both the prick and specific IgE tests efficiently detected sensitisation to opiates. The highest levels were related to more-severe clinical profiles.
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Anafilaxia/diagnóstico , Codeína/análogos & derivados , Hipersensibilidad a las Drogas/diagnóstico , Inmunoglobulina E/sangre , Morfina , Morfolinas , Papaver/inmunología , Trastornos Relacionados con Sustancias/complicaciones , Trastornos Relacionados con Sustancias/inmunología , Adolescente , Adulto , Anciano , Anafilaxia/complicaciones , Estudios de Casos y Controles , Niño , Codeína/efectos adversos , Codeína/inmunología , Hipersensibilidad a las Drogas/complicaciones , Femenino , Humanos , Masculino , Persona de Mediana Edad , Morfina/efectos adversos , Morfina/inmunología , Morfolinas/efectos adversos , Morfolinas/inmunología , Opio/administración & dosificación , Papaver/efectos adversos , Valor Predictivo de las Pruebas , Semillas , Sensibilidad y Especificidad , Pruebas Cutáneas , Nicotiana/inmunología , Adulto JovenAsunto(s)
Aldehído-Liasas/inmunología , Artemia/inmunología , Peces/inmunología , Hipersensibilidad a los Alimentos/diagnóstico , Hipersensibilidad a los Alimentos/inmunología , Inmunoglobulina E/inmunología , Hipersensibilidad Respiratoria/diagnóstico , Hipersensibilidad Respiratoria/inmunología , Animales , Artemia/enzimología , Electroforesis en Gel Bidimensional , Femenino , Hipersensibilidad a los Alimentos/terapia , Humanos , Inmunoglobulina E/sangre , Persona de Mediana Edad , Hipersensibilidad Respiratoria/terapiaRESUMEN
BACKGROUND: Over the last decades, genomics and proteomics have contributed to the current knowledge of individualized allergenic components and their potential use in the diagnosis of IgE-mediated allergies. Recent investigations have demonstrated that Alt a 1 should be considered as a relevant allergen of the Pleosporaceae group and that enolase is the main allergen involved in the cross-reactivity to fungi. However, the real utility of these allergens as tools for the diagnosis of allergy to Alternaria is still unclear. OBJECTIVE: To demonstrate the current value of the available fungal allergen panel and the need to build an accurate mould allergen array for the diagnosis of allergy to Pleosporaceae. METHODS: Specific IgEs to individual mould allergens and allergenic mould extracts were evaluated using the ImmunoCAP™ system in 30 patients allergic to Alternaria and in 100 blood donors. Cross-reactivity studies were performed by Fluoro Enzyme ImmunoAssay (FEIA) and FEIA inhibition using individual allergens and allergenic extracts. Two-dimensional electrophoresis associated with a MALDI-TOF analysis was carried out to identify new allergen molecules. RESULTS: All allergic patients had positive specific IgE responses to several moulds from different taxonomical families. Classic and molecular diagnosis demonstrated that 23% of patients had multi-sensitization. The current commercially available fungal allergen array was not sufficient to establish an accurate diagnosis. Unexpected correlations between Alternaria or Alt a 1 and Curvularia or Cladosporium stimulated the investigation of a more accurate allergen panel. A manganese-dependent superoxide dismutase (MnSOD) homologous to Asp f 6 was identified as a new IgE-binding molecule from Alternaria alternata. CONCLUSIONS AND CLINICAL RELEVANCE: Alt a 1 is the marker for allergy to Pleosporaceae, not including Curvularia. MnSOD can explain 6.6% of allergy to Alternaria without Alt a 1 sensitization and should be included together with Alt a 1 and fungal enolase in the molecular array for the diagnosis of allergy to Pleosporaceae.
Asunto(s)
Antígenos Fúngicos/inmunología , Antígenos de Plantas/inmunología , Ascomicetos/inmunología , Hongos/inmunología , Hipersensibilidad/diagnóstico , Hipersensibilidad/inmunología , Superóxido Dismutasa/inmunología , Adolescente , Adulto , Alérgenos , Ascomicetos/enzimología , Reacciones Cruzadas , Electroforesis en Gel Bidimensional , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Técnicas Inmunológicas , Masculino , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Adulto JovenRESUMEN
BACKGROUND: Cannabis is the illicit drug most widely used by young people in high-income countries. Allergy symptoms have only occasionally been reported as one of the adverse health effects of cannabis use. OBJECTIVES: To study IgE-mediated response to cannabis in drug users, atopic patients, and healthy controls. METHODS: Asthmatic patients sensitised to pollen, and all patients sensitised to tobacco, tomato and latex, considered as cross-reacting allergens, were selected from a data base of 21,582 patients. Drug users attending a drug-rehabilitation clinic were also included. Controls were 200 non-atopic blood donors. Specific IgE determination, prick tests and specific challenge with cannabis extracts were performed in patients and controls. RESULTS: Overall, 340 patients, mean age 26.9±10.7 years, were included. Males (61.4%) were the most sensitised to cannabis (p<0.001). All cannabis-sensitised patients were alcohol users. Eighteen (72%) of the patients allergic to tomato were sensitised to cannabis, but a positive specific challenge to cannabis was highest in patients sensitised to tobacco (13/21, 61.9%), (p<0.001). Pollen allergy was not a risk factor for cannabis sensitisation. Prick tests and IgE for cannabis had a good sensitivity (92 and 88.1%, respectively) and specificity (87.1 and 96%) for cannabis sensitisation. CONCLUSIONS: Cannabis may be an important allergen in young people. Patients previously sensitised to tobacco or tomato are at risk. Cannabis prick tests and IgE were useful in detecting sensitisation.
Asunto(s)
Alérgenos/inmunología , Antígenos de Plantas/inmunología , Asma/inmunología , Cannabis , Grupos de Población , Adolescente , Adulto , Alérgenos/efectos adversos , Asma/diagnóstico , Asma/epidemiología , Cannabis/inmunología , Reacciones Cruzadas , Femenino , Humanos , Drogas Ilícitas/inmunología , Inmunoglobulina E/inmunología , Solanum lycopersicum/inmunología , Masculino , Polen/efectos adversos , Riesgo , Sensibilidad y Especificidad , Pruebas Cutáneas , España , Nicotiana/inmunologíaRESUMEN
BACKGROUND: Component-resolved diagnosis based on recombinant allergens facilitates treatment of multiple sensitization and/or crossreactivity in allergic patients. OBJECTIVE: To assess the usefulness of molecular diagnosis in childhood allergies. METHODS: A total of 162 children aged 4-16 years diagnosed with allergic rhinitis or asthma/rhinitis caused by pollen were referred for recombinant allergen-based diagnosis in 2006. Specific immunoglobulin (Ig) E against pollen allergens and purified recombinant Phleum pratense pollen allergens were measured using an in vitro quantitative assay, and considering the recombinant allergens Phl p 1+Phl p 5 as P pratense--specific allergens and Phl p 7+Phl p 12 as cross-reacting allergens. Conditional probability was calculated to determine the relationship between values for specific IgE against major allergens and those for cross-reacting allergens. RESULTS: Specific IgE antibodies against P pratense were detected in 99.4% of serum samples, and cross-reacting allergens in 46%. Multiple sensitization to pollen was documented in 38% of patients, with Plantago lanceolata as the main cause. Conditional probability calculations showed that patients with specific IgE values of 75-80 kU(A)/L to Phl p 1+Phl p 5 were 75% (95% confidence interval) more likely to present values > or = 2 kUA/L to Phl p 7+Phl p 12. CONCLUSIONS: Our results show that recombinant DNA technology can help diagnose allergy in cases of multiple sensitization and crossreactivity, and is therefore a promising option for improving prognosis and management of allergic pediatric populations.
Asunto(s)
Antígenos de Plantas/inmunología , Patología Molecular/métodos , Polen/inmunología , Proteínas Recombinantes/inmunología , Rinitis Alérgica Estacional/diagnóstico , Adolescente , Antígenos de Plantas/genética , Antígenos de Plantas/metabolismo , Niño , Preescolar , Reacciones Cruzadas , Errores Diagnósticos , Femenino , Humanos , Masculino , Phleum/inmunología , Polen/efectos adversos , Pronóstico , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Rinitis Alérgica Estacional/etiología , Rinitis Alérgica Estacional/inmunología , Rinitis Alérgica Estacional/terapiaRESUMEN
The date palm (Phoenix dactylifera) has a wide geographical distribution (Middle East, Mediterranean, central Africa, western Asia, Australia, and North America). Pho d 2, the major allergen of date palm pollen was recently identified as a profilin, yet little is known about the nature of the other pollen allergens from this tree. The objective of this study was to characterize clinically significant allergens other than profilins from P. dactylifera pollen using immunoproteomics. In order to reveal the proteins causing the allergy, we used serum from a patient monosensitized to date palm pollen extract who experienced asthma and rhinoconjunctivitis during the palm tree pollen season. The results revealed 2 novel immunoglobulin E-binding proteins not related to the cross-reactive allergen profilin. Individualized allergens of Pdactylifera that cause specific date palm pollen sensitization must be defined to determine the real prevalence of sensitization to this species.
Asunto(s)
Antígenos de Plantas/inmunología , Asma/diagnóstico , Rinitis Alérgica Estacional/diagnóstico , Adulto , Alérgenos/inmunología , Antígenos de Plantas/sangre , Antígenos de Plantas/genética , Antígenos de Plantas/aislamiento & purificación , Asma/complicaciones , Asma/inmunología , Asma/fisiopatología , Conjuntivitis , Electroforesis en Gel Bidimensional , Femenino , Frutas/inmunología , Galactosidasas/genética , Glucosiltransferasas/genética , Humanos , Inmunización , Proteómica , Rinitis , Rinitis Alérgica Estacional/complicaciones , Rinitis Alérgica Estacional/inmunología , Rinitis Alérgica Estacional/fisiopatologíaRESUMEN
Dirofilaria immitis is the agent of the heartworm disease in canids and felids, and of pulmonary dirofilariosis in man. Like other filariae, D. immitis harbours endosymbion Wolbachia bacteriae. In this work we analyse the response of specific IgE antibodies against both D. immitis antigens and the Wolbachia surface protein (WSP) in two groups of persons living in an area of canine endemia, one presenting high levels of total IgE (group 1) and other with normal levels (group 2). Infections with D. immitis were demonstrated by the presence of specific IgG in 228 individuals(48.8%) of the group 1 and only in one of the group 2. Specific IgE antibody response against D. immitis antigens was detected only in individuals of the group 1. IgE response against WSP was not detected in any group. The IgE response was directed mainly against two molecules of 33 and 42 kDa of the antigenic extract of D. immitis. These molecules were identified by mass spectrometry as a galectin and an aldolase, respectively. Their possible role in the survival mechanisms of the parasite and their contribution to development of allergic reactions in individuals resident in areas with heartworm disease are discussed.
Asunto(s)
Aldehído-Liasas/inmunología , Anticuerpos Antihelmínticos/sangre , Antígenos Helmínticos/inmunología , Dirofilaria immitis/inmunología , Galectinas/inmunología , Inmunoglobulina E/sangre , Wolbachia/inmunología , Animales , Anticuerpos Antibacterianos/sangre , Antígenos Helmínticos/química , Dirofilaria immitis/microbiología , Dirofilaria immitis/fisiología , Dirofilariasis/inmunología , Proteínas del Helminto/química , Proteínas del Helminto/inmunología , Humanos , Inmunoglobulina G/sangre , Enfermedades Pulmonares/inmunología , Masculino , Espectrometría de Masas , Peso Molecular , Simbiosis/inmunología , Wolbachia/fisiologíaRESUMEN
The ubiquitously present spores of Alternaria alternata can spoil a wide variety of foodstuffs, including a variety of fruits belonging to the Citrus genus. The major allergenic protein of A. alternata, Alt a 1, is a species-specific molecular marker that has been strongly associated with allergenicity and phytopathogenicity of this fungal species. This study aimed to evaluate the potential of the detection of Alt a 1 as a reliable indicator of A. alternata contamination in citrus fruits. To accomplish this aim, sixty oranges were artificially infected with a spore suspension of A. alternata. Internal fruit material was collected at different incubation times (one, two and three weeks after the fungal inoculation) and used for both total RNA extraction and protein extraction. Alt a 1 detection was then performed by polymerase chain reaction (PCR) amplification using Alt a 1 specific primers and by enzyme-linked immunosorbent assay (ELISA). The experimental model presented in this work was effective to simulate the typical Alternaria black rot phenotype and its progression. Although both PCR and ELISA techniques have been successfully carried out for detecting Alt a 1 allergen in A. alternata infected oranges, the PCR method was found to be more sensitive than ELISA. Nevertheless, ELISA results were highly valuable to demonstrate that considerable amounts of Alt a 1 are produced during A. alternata fruit infection process, corroborating the recently proposed hypothesis that this protein plays a role in the pathogenicity and virulence of Alternaria species. Such evidence suggests that the detection of Alt a 1 by PCR-based assay may be used as a specific indicator of the presence of pathogenic and allergenic fungal species, A. alternata, in fruits. This knowledge can be employed to control the fungal infection and mitigate agricultural losses as well as human exposure to A. alternata allergens and toxins.
Asunto(s)
Alternaria/aislamiento & purificación , Antígenos Fúngicos/análisis , Citrus/microbiología , Ensayo de Inmunoadsorción Enzimática/métodos , Contaminación de Alimentos/análisis , Proteínas Fúngicas/análisis , Alternaria/inmunología , Antígenos Fúngicos/inmunología , Frutas/microbiología , Humanos , Esporas Fúngicas/inmunología , Esporas Fúngicas/aislamiento & purificaciónRESUMEN
BACKGROUND: While it is well known that there is significant intraspecific variation in the content an potency of Alternaria alternata allergens, little data has been published on intraspecific variability for individual allergens from moulds. OBJECTIVE: To assess the variability of Alt a 1 expression in different strains of A alternata. METHODS: Eleven strains of A alternata were cultured in a Czapek broth medium and culture filtrate extracts were obtained. A sensitive two-site enzyme-linked immunosorbent assay was used to measure Alt a 1 concentrations in medium following 3 weeks of culture and in culture filtrate extracts. RESULTS: Expression of Alt a 1 was highly variable in different strains ofA alternata (coefficient of variation > 135%). A good correlation was found between Alt a 1 concentrations at the beginning of the process and measurements at the end of extract production (r=0.940). CONCLUSIONS: The high variability of Alt a 1 expression in different A alternata strains makes it necessary to measure Alt a 1 concentrations during the first stage of allergenic extract production in order to be able to choose a suitable strain for producing extracts or purifying Alt a 1.
Asunto(s)
Alérgenos/análisis , Alternaria/inmunología , Ensayo de Inmunoadsorción EnzimáticaRESUMEN
BACKGROUND: Barnacles are a type of seafood with worldwide distribution and abundant along the shores of temperate seas. They are particularly appreciated and regularly consumed in Portugal as well as in Spain, France and South America, but barnacle allergy is a rare condition of which there is only one reference in the indexed literature. The molecular allergens and possible cross-reactivity phenomena implicated (namely with mites) have not been established. OBJECTIVE: To demonstrate the IgE-mediated allergy to barnacle and to identify the proteins implicated as well as possible cross-reactivity phenomena with mites. METHODS: We report the clinical and laboratory data of five patients with documented IgE-mediated allergy to barnacle. The diagnosis was based on a suggestive clinical history combined with positive skin prick tests (SPT) to barnacle--prick to prick method. Two barnacle extracts were prepared (raw and cooked barnacle) and sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and IgE-immunoblotting were performed. An immunoblotting inhibition assay with Dermatophagoides pteronyssinus was also done in order to evaluate cross-reactivity. RESULTS: All patients had mite-related asthma and the allergic rhinoconjunctivitis; they all experienced mucocutaneous symptoms. All of them had positive SPT to barnacle, and the immunoblotting showed several allergenic fractions with a wide molecular weight range (19 - 94 kDa). The D. pteronyssinus extract inhibited several IgE-binding protein fractions in the barnacle extract. CONCLUSIONS: We describe five patients with IgE-mediated barnacle allergy. We also describe a group of IgE-binding+proteins between 30 and 75 kDa as the allergenic fractions of this type of Crustacea. Cross-reactivity with D. pteronyssinus was demonstrated in two cases.
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Antígenos Dermatofagoides/inmunología , Asma/inmunología , Hipersensibilidad a los Alimentos , Rinitis Alérgica Perenne/inmunología , Thoracica/inmunología , Adolescente , Alérgenos/inmunología , Animales , Asma/sangre , Niño , Preescolar , Reacciones Cruzadas , Dermatophagoides pteronyssinus/inmunología , Femenino , Humanos , Inmunoglobulina E/sangre , Inmunoglobulina E/inmunología , Masculino , Rinitis Alérgica Perenne/sangre , Mariscos/efectos adversos , Pruebas CutáneasRESUMEN
Alternaria alternata is one of the most common saprophytes worldwide that is clinically and epidemiologically associated with severe asthma. Therefore, the identification and characterization of all A. alternata allergens are of major clinical importance. This study describes a new cross-reactive A. alternata allergen that was officially named Alt a 15 by the official Allergen Nomenclature Subcommittee. The complete coding region for Alt a 15 was amplified using 5' and 3' rapid amplification of cDNA ends and PCR. The recombinant protein was produced in Escherichia coli as a 65-kDa fusion protein, and the protein sequence exhibits high homology with several important fungal allergens. Immunoblotting analyses revealed that IgE antibodies from A. alternata-sensitized patients (n=59) bound to rAlt a 15 with a prevalence of 10.2%. All patients who presented sIgE to rAlt a 15 were apparently poly-sensitized to A. alternata and C. lunata. The extensive cross-reactivity between A. alternata and C. lunata serine proteases was confirmed using immunoblotting inhibition assays. Overall, Alt a 15 is an important new cross-reactive allergen of A. alternata that explains some allergies to A. alternata without Alt a 1 sensitization and initial diagnostic errors for allergies to Alternaria. This molecule may improve the accuracy of the diagnosis, the understanding, and the management of IgE-mediated fungal diseases.
Asunto(s)
Alérgenos/inmunología , Alternaria/inmunología , Anticuerpos Antifúngicos/química , Antígenos Fúngicos/inmunología , Asma/inmunología , Inmunoglobulina E/química , Alérgenos/química , Alérgenos/genética , Alternaria/química , Secuencia de Aminoácidos , Anticuerpos Antifúngicos/aislamiento & purificación , Especificidad de Anticuerpos , Antígenos Fúngicos/química , Antígenos Fúngicos/genética , Asma/inducido químicamente , Asma/genética , Asma/microbiología , Clonación Molecular , Reacciones Cruzadas , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Humanos , Inmunoglobulina E/aislamiento & purificación , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Unión Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Alineación de SecuenciaRESUMEN
We report three new cases of allergy to Gammarus, two of them involving cutaneous symptoms, and the third one with occupational asthma. The results showed exposure to feed containing Gammarus shrimp to be the main cause of the allergic symptoms in the three patients. In all cases the intervention of IgE was demonstrated, with the absence of cross-reactions with other common allergenic arthropods.
Asunto(s)
Anfípodos/inmunología , Hipersensibilidad a los Alimentos/etiología , Adolescente , Adulto , Animales , Niño , Humanos , Inmunoglobulina E/sangre , MasculinoAsunto(s)
Anafilaxia/epidemiología , Fertilización In Vitro , Hipersensibilidad Inmediata/inmunología , Inmunoensayo , Inmunoglobulina E/inmunología , Anafilaxia/etiología , Anafilaxia/prevención & control , Animales , Bovinos , Comorbilidad , Diagnóstico Precoz , Epítopos , Femenino , Fertilización In Vitro/efectos adversos , Humanos , Hipersensibilidad Inmediata/diagnóstico , Hipersensibilidad Inmediata/etiología , Inmunoglobulina E/sangre , Factores de Riesgo , Albúmina Sérica Bovina , EspañaRESUMEN
BACKGROUND: At present, data about the cross-reactivity of Blomia spp. comes from studies made among different genera of mites, and no results have been published involving different species of the genus Blomia. OBJECTIVE: The aim of this study was to find out the level of cross-reactivity between the two main species of Blomia causing allergy, and its implication in the diagnosis of Blomia sensitization. METHODS: Using extracts from optimal growth phases of Blomia kulagini (Zakhvatkin, 1936) and Blomia tropicalis (van Bronswijk, Cock and Oshima, 1973) as allergenic material, the allergenic cross-reactivity between both house dust mites was evaluated by means of cutaneous tests, specific IgE values, ImmunoCAP-inhibition and SDS-PAGE-IgE-immunoblotting-inhibition. RESULTS: The results demonstrated that IgE-binding components belonging to both species are very similar from the immunological point of view, showing high correlations between both species when using cutaneous tests (R2=0.915) or specific IgE (R2=0.980). ImmunoCAP-inhibition and SDS-PAGE-IgE-Immunoblotting-inhibition probed with human sera, showed a total inhibition of specific IgE reactions by the heterologous antigens. CONCLUSION: The results obtained strongly suggest the great resemblance between the allergenic composition of both species.
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Ácaros y Garrapatas/inmunología , Alérgenos/inmunología , Ácaros y Garrapatas/química , Ácaros y Garrapatas/clasificación , Animales , Colombia , Reacciones Cruzadas/inmunología , Humanos , Inmunoglobulina E/sangre , Inmunoglobulina E/inmunología , Pruebas CutáneasRESUMEN
We report a new case of rhinitis and asthma caused by sea fishing baits. The results showed exposure to Sipunculus nudus (Phylum Sipuncula; order Sipunculida: Sipunculidae) to be the main cause of the allergic symptoms. The intervention of IgE was demonstrated, with the presence of cross-reactions with allergenic extracts from other worm species used as baits, belonging to different orders of Annelida.
Asunto(s)
Alérgenos/efectos adversos , Asma/etiología , Nematodos/inmunología , Rinitis Alérgica Perenne/etiología , Alérgenos/inmunología , Animales , Especificidad de Anticuerpos , Asma/inmunología , Reacciones Cruzadas/inmunología , Femenino , Humanos , Immunoblotting , Inmunoglobulina E/sangre , Inmunoglobulina E/inmunología , Persona de Mediana Edad , Nematodos/aislamiento & purificación , Océanos y Mares , Oligoquetos/inmunología , Poliquetos/inmunología , Rinitis Alérgica Perenne/inmunología , Pruebas CutáneasRESUMEN
No disponible
Asunto(s)
Humanos , Masculino , Persona de Mediana Edad , Reacciones Cruzadas/inmunología , Hipersensibilidad a los Alimentos/diagnóstico , Hipersensibilidad Respiratoria/diagnóstico , Artemia , Pruebas Cutáneas , PecesRESUMEN
Although Uncinaria stenocephala is the most frequent hookworm in the intestine of dogs from Northern, Central and Southern Europe, little is known about its host-parasite relationship. Three groups of sera from dogs (Group 1: dogs naturally infected only by U. stenocephala; Group 2: helminth-free dogs at necropsy, and Group 3: dogs parasitized by other helminths) were analyzed by ELISA using U. stenocephala antigens from adult worms (somatic and excretory-secretory antigens) and from L3 larvae (somatic antigens). All three sources of antigens were found to be suitable for immunodiagnosis of canine uncinariosis with up to 90% efficacy. However, an analysis to assess the diagnostic value of the different antigens demonstrated that the adult excretory-secretory antigens had a higher diagnostic efficacy (96.7%), indicating that this is the best antigen source for the diagnosis of Uncinaria infection.
Asunto(s)
Ancylostomatoidea/inmunología , Anticuerpos Antihelmínticos/sangre , Antígenos Helmínticos/inmunología , Enfermedades de los Perros/diagnóstico , Infecciones por Uncinaria/veterinaria , Animales , Perros , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Infecciones por Uncinaria/diagnóstico , Interacciones Huésped-Parásitos/inmunología , Sueros Inmunes/inmunología , Intestino Delgado/parasitología , Larva/inmunología , Masculino , Curva ROCRESUMEN
There is general consensus regarding the scarce cross-reactivity existing between Alternaria alternata and other allergenic moulds such as Aspergillus fumigatus, Penicillium notatum or Cladosporium herbarum. However, A. alternata has been shown to have a very significant level of allergenic cross-reactivity with other fungi belonging to the Pleosporaceae family. To date, no biological identity or homologies with other proteins have been described for Alt a 1, and it remains unclear whether the major allergen Alt a 1 contributes to the cross-reactivity shown for these moulds. Specific quantification of Alt a 1 in culture filtrates of Stemphylium botryosum, Ulocladium botrytis, Curvularia lunata, Alternaria tenuissima, C. herbarum, Penicillium chrysogenum and Asp. fumigatus, and immunoblotting using culture filtrate extracts from the above-mentioned moulds and rabbit serum anti-recombinant Alt a 1 have shown significant amounts of Alt a 1 in culture filtrates as well as antigenic components ranging from 14 to 20 kDa that strongly react with the specific serum for all taxonomically related species (Pleosporaceae family). No reactions were revealed in culture filtrates of Cladosporium, Penicillium and Aspergillus. These results restrict the cross-reactivity phenomenon due to Alt a 1 to the scope of the taxonomical term of family.