RESUMEN
Fossil wood is the naturally preserved remain of the secondary xylem of plants that lived before the Holocene epoch. Typically, fossil wood is preserved as coalified or petrified and rarely as mummified tissue. The process of fossilization is very complex and it is still unknown why in the same fossil record, wood can be found in different fossilisation forms. In 2007, a fossil forest was found in the Bükkábrány open-pit coal mine in Hungary. The non-petrified forest is estimated to be 7 million years old (Miocene epoch) and its trees were found standing in an upright position. This fossil assemblage is exceptionally rare because wood has been preserved as soft waterlogged tissue. This study aimed to investigate this remarkable way of fossil wood preservation, by examining its chemistry with (13)C CPMAS NMR and its morphology with light and electron microscopy. For comparison reasons, a petrified wood trunk from the Oligocene epoch (30 Myr) found in 2001 at Porrentruy region in Switzerland and two fresh wood samples of the modern equivalents of the Miocene sample were also examined. The results obtained showed that the outstanding preservation state of the Miocene fossil is not owed to petrification or coalification. Mummification is a potential mechanism that could explain Bükkábrány trunks' condition, however this fossilisation process is not well studied and therefore this hypothesis needs to be further investigated.
RESUMEN
Paper documents in archives, libraries, and museums often undergo biodeterioration by microorganisms. Fungi and less often bacteria have been described to advance paper staining, so called "foxing" and degradation of paper substrates. In this study, for the first time, the fungal and bacterial diversity in biodeteriorated paper documents of Hellenic General State Archives dating back to the 19th and 20th century has been assessed by culture-dependent and independent methods. The internally transcribed spacer (ITS) region and 16S rRNA gene were amplified by PCR from fungal and bacterial isolates and amplicons were sequenced. Sequence analysis and phylogeny revealed fungal phylotypes like Penicillium sp., Cladosporium sp., Penicillium citrinum, Alternaria infectoria, Alternaria alternata, Epicoccum nigrum, and Penicillium chrysogenum which are often implicated in paper deterioration. Bacterial phylotypes closely related to known biodeteriogenic bacteria such as Bacillus spp., Micrococcus spp., Kocuria sp. in accordance with previous studies were characterized. Among the fungal phylotypes described in this study are included well-known allergens such as Penicillium spp., Alternaria spp., and Cladosporium spp. that impose a serious health threat on staff members and scholars. Furthermore, fungal isolates such as Chalastospora gossypii and Trametes ochracea have been identified and implicated in biodeterioration of historical paper manuscripts in this study for the first time. Certain new or less known fungi and bacteria implicated in paper degradation were retrieved, indicating that particular ambient conditions, substrate chemistry, or even location might influence the composition of colonizing microbiota.