A New Coumarin-Acridone Compound as a Fluorescence Probe for Fe3+ and Its Application in Living Cells and Zebrafish.

A new coumarin-acridone fluorescent probe S was designed and synthesized, and the structure was confirmed with 1H/13C NMR spectrometry, single-crystal X-ray diffraction, and high-resolution mass spectrometry. This probe has high sensitivity and selectivity for Fe3+ over other testing metal ions at 420 or 436 nm in acetonitrile-MOPS (3-Morpholinopropanesulfonic Acid) buffer solution (20.0 µM, pH = 6.9, 8:2 (v/v)). Under physiological conditions, the probe displayed satisfying time stability with a detection limit of 1.77 µM. In addition, probe S was successfully used to detect intracellular iron changes through a fluorescence-off mode, and the imaging results of cells and zebrafish confirmed their low cytotoxicity and satisfactory cell membrane permeability, as well as their potential biological applications.

The role of product cues and regulatory focus in the consumers' response to green products: The mediation effects of green attitudes.

By applying the cue-diagnosticity theory, this study explores the influence mechanism of consumption response to low-involvement products and high-involvement products, respectively. Specifically, the purpose of this study is to investigate how product clues (brand strength and retailer reputation) affect consumption responses to green products with different involvement and to examine regulatory focus as a moderator and green attitude as a mediator. The results of study 1 reveal that for low-involvement green products, the effect of the retailer reputation rather than brand strength on consumption response is mediated by a green attitude, and the regulatory focus plays a moderating role in this process. The results of study 2 show that for high-involvement green products, the effect of the brand strength rather than retailer reputation on consumption response is mediated by a green attitude; however, the regulatory focus does not play a moderating role in this process. Finally, the data aggregation verifies that people's consumption response to green products strongly depends on the retailer reputation, brand strength, and green attitude, and there is a moderated mediation effect of regulatory focus on the indirect effect of retailer reputation (rather than brand strength) on consumption response via green attitude. As behavioral antecedents differ across the analyzed product types in forming consumer response, it is very important for policymakers and marketers to take note of the differences when designing marketing activities for green products.

Cluster randomization trial of sequence mass screening for colorectal cancer.

PURPOSE: Colorectal cancer is a major cause of death worldwide. To reduce the incidence and mortality from rectal cancer, an individual quantitative risk-assessment model (hereafter referred to as the Attributive Degree Value) and reverse passive hemagglutination fecal occult blood test were used in a randomized, controlled, population-based trial that was conducted in Jiashan County, People's Republic of China. METHODS: All residents of Jiashan County aged 30 years or older were enrolled in the study, and 21 townships in the county were randomized to either a screening (n = 10 townships) or control (n = 11 townships) group. Participants in the screened group submitted a one-article-per-slide stool sample and completed a structured risk-assessment questionnaire from which their attributive degree value was computed. According to study protocol, 4,299 participants were defined as high risk and underwent diagnostic evaluation with 60-cm flexible sigmoidoscopy and, in some cases, an additional screening with colonoscopy. RESULTS: From 1989 to 1996, cumulative mortality from colon cancer was 90 (95 percent confidence interval, 83-97) per 100,000 in the screened group and 83 (95 percent confidence interval, 76-90) per 100,000 in the control group (log-rank P = 1.49, P = 0.222). Mortality from rectal cancer during this time was 110 (95 percent confidence interval, 102-118) per 100,000 in the screened group, which differed significantly from the control group mortality rate of 161 (95 percent confidence interval, 152-170) per 100,000 (log-rank P = 0.003). CONCLUSION: Mass screening with a reverse passive hemagglutination fecal occult blood test along with an individual attributive degree value score was effective in reducing mortality from rectal cancer but not in reducing mortality from colon cancer or the incidence of colorectal cancer.