RESUMEN
Globalization has led to the introduction of thousands of alien species worldwide. With growing impacts by invasive species, understanding the invasion process remains critical for predicting adverse effects and informing efficient management. Theoretically, invasion dynamics have been assumed to follow an "invasion curve" (S-shaped curve of available area invaded over time), but this dynamic has lacked empirical testing using large-scale data and neglects to consider invader abundances. We propose an "impact curve" describing the impacts generated by invasive species over time based on cumulative abundances. To test this curve's large-scale applicability, we used the data-rich New Zealand mud snail Potamopyrgus antipodarum, one of the most damaging freshwater invaders that has invaded almost all of Europe. Using long-term (1979-2020) abundance and environmental data collected across 306 European sites, we observed that P. antipodarum abundance generally increased through time, with slower population growth at higher latitudes and with lower runoff depth. Fifty-nine percent of these populations followed the impact curve, characterized by first occurrence, exponential growth, then long-term saturation. This behaviour is consistent with boom-bust dynamics, as saturation occurs due to a rapid decline in abundance over time. Across sites, we estimated that impact peaked approximately two decades after first detection, but the rate of progression along the invasion process was influenced by local abiotic conditions. The S-shaped impact curve may be common among many invasive species that undergo complex invasion dynamics. This provides a potentially unifying approach to advance understanding of large-scale invasion dynamics and could inform timely management actions to mitigate impacts on ecosystems and economies.
Asunto(s)
Ecosistema , Especies Introducidas , Animales , Europa (Continente) , Nueva Zelanda , CaracolesRESUMEN
Pyrethroid insecticides are known to be highly toxic to most aquatic nontarget organisms, but little is known about the mechanisms causing some species to be highly sensitive while others are hardly affected by the pyrethroids. The aim of the present study was to measure the sensitivity (EC50-values) of 10 aquatic invertebrates toward a 24 h pulse of the pyrethroid cypermethrin and subsequently test if the difference in sensitivity could be explained by measured morphological and physiological traits and modeled toxicokinetic (TK) and toxicodynamic (TD) parameters. Large differences were observed for the measured uptake and elimination kinetics, with bioconcentration factors (BCFs) ranging from 53 to 2337 at the end of the exposure. Similarly, large differences were observed for the TDs, and EC50-values after 168 h varied 120-fold. Modeling the whole organism cypermethrin concentrations indicated compartmentation into a sorbed fraction and two internal fractions: a bioavailable and non-bioavailable internal fraction. Strong correlations between surface/volume area and the TK parameters (sorption and uptake rate constants and the resulting BCF) were found, but none of the TK parameters correlated with sensitivity. The only parameter consistently correlating with sensitivity across all species was the killing rate constant of the GUTS-RED-SD model (the reduced general unified threshold models of survival assuming stochastic death), indicating that sensitivity toward cypermethrin is more related to the TD parameters than to TK parameters.
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Piretrinas , Contaminantes Químicos del Agua , Animales , Organismos Acuáticos , Invertebrados , Cinética , ToxicocinéticaRESUMEN
Fungicides are indispensable to global food security and their use is forecasted to intensify. Fungicides can reach aquatic ecosystems and occur in surface water bodies in agricultural catchments throughout the entire growing season due to their frequent, prophylactic application. However, in comparison to herbicides and insecticides, the exposure to and effects of fungicides have received less attention. We provide an overview of the risk of fungicides to aquatic ecosystems covering fungicide exposure (i.e., environmental fate, exposure modeling, and mitigation measures) as well as direct and indirect effects of fungicides on microorganisms, macrophytes, invertebrates, and vertebrates. We show that fungicides occur widely in aquatic systems, that the accuracy of predicted environmental concentrations is debatable, and that fungicide exposure can be effectively mitigated. We additionally demonstrate that fungicides can be highly toxic to a broad range of organisms and can pose a risk to aquatic biota. Finally, we outline central research gaps that currently challenge our ability to predict fungicide exposure and effects, promising research avenues, and shortcomings of the current environmental risk assessment for fungicides.
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Fungicidas Industriales , Plaguicidas , Contaminantes Químicos del Agua , Animales , Ecosistema , InvertebradosRESUMEN
We exposed 34 species of stream macroinvertebrates, representing 29 families, to a 90 min pulse of the pyrethroid λ-cyhalothrin. For 28 of these species, no pyrethroid ecotoxicity data exist. We recorded mortality rates 6 days post-exposure, and the behavioral response to pyrethroid exposure was recorded using automated video tracking. Most arthropod species showed mortality responses to the exposure concentrations (0.01-10 µg L(-1)), whereas nonarthropod species remained unaffected. LC50 varied by at least a factor of 1000 among arthropod species, even within the same family. This variation could not be predicted using ecotoxicity data from closely related species, nor using species-specific indicator values from traditional ecological quality indices. Moreover, LC50 was not significantly correlated to effect thresholds for behavioral responses. Importantly, however, the measured surface area-weight ratio and the preference for coarse substrates significantly influenced the LC50 for arthropod species, with the combination of small individuals and strong preference for coarse substrates indicating higher pyrethroid sensitivity. Our study highlights that existing pesticide ecotoxicity data should be extrapolated to untested species with caution and that actual body size (not maximum potential body size, as is usually available in traits databases) and habitat preference are central parameters determining species sensitivities to pyrethroids.
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Insecticidas/toxicidad , Contaminantes Químicos del Agua/toxicidad , Agua Dulce , Humanos , Plaguicidas/toxicidad , Piretrinas/toxicidadRESUMEN
Legislative and managing entities of EU member states face a comprehensive task because the chemical and ecological impacts of contaminated sites on surface waters must be assessed. The ecological assessment is further complicated by the low availability or, in some cases, absence of ecotoxicity data for many of the compounds occurring at contaminated sites. We studied the potential impact of a contaminated site, characterised by chlorinated solvents, sulfonamides, and barbiturates, on benthic macroinvertebrates in a receiving stream. Most of these compounds are characterised by low or unknown ecotoxicity, but they are continuously discharged into the stream by way of a long-lasting source generating long-term chronic exposure of the stream biota. Our results show that taxonomical density and diversity of especially sediment dwelling taxa were reduced by >50 % at the sampling sites situated in the primary inflow zone of the contaminated GW. Moreover, macroinvertebrate communities at these sampling sites could be distinguished from those at upstream control sites and sites situated along a downstream dilution gradient using multidimensional scaling. Importantly, macroinvertebrate indices currently used did not identify this impairment, thus underpinning an urgent need for developing suitable tools for the assessment of ecological effects of contaminated sites in streams.
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Monitoreo del Ambiente , Agua Subterránea/química , Invertebrados/fisiología , Contaminantes Químicos del Agua/análisis , Animales , Industria Química , Residuos Industriales , Ríos/química , Contaminantes Químicos del Agua/toxicidadRESUMEN
Humans impact terrestrial, marine and freshwater ecosystems, yet many broad-scale studies have found no systematic, negative biodiversity changes (for example, decreasing abundance or taxon richness). Here we show that mixed biodiversity responses may arise because community metrics show variable responses to anthropogenic impacts across broad spatial scales. We first quantified temporal trends in anthropogenic impacts for 1,365 riverine invertebrate communities from 23 European countries, based on similarity to least-impacted reference communities. Reference comparisons provide necessary, but often missing, baselines for evaluating whether communities are negatively impacted or have improved (less or more similar, respectively). We then determined whether changing impacts were consistently reflected in metrics of community abundance, taxon richness, evenness and composition. Invertebrate communities improved, that is, became more similar to reference conditions, from 1992 until the 2010s, after which improvements plateaued. Improvements were generally reflected by higher taxon richness, providing evidence that certain community metrics can broadly indicate anthropogenic impacts. However, richness responses were highly variable among sites, and we found no consistent responses in community abundance, evenness or composition. These findings suggest that, without sufficient data and careful metric selection, many common community metrics cannot reliably reflect anthropogenic impacts, helping explain the prevalence of mixed biodiversity trends.
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Biodiversidad , Ecosistema , Animales , Humanos , Invertebrados , Ríos , Europa (Continente)RESUMEN
Reliable characterization of exposure is indispensable for ecological risk assessment of chemicals. To deal with mixtures, several approaches have been developed, but their relevance for predicting ecological effects on communities in the field has not been elucidated. In the present study, we compared nine metrics designed for estimating the total toxicity of mixtures regarding their relationship with an effect metric for stream macroinvertebrates. This was done using monitoring data of biota and organic chemicals, mainly pesticides, from five studies comprising 102 streams in several regions of Europe and South-East Australia. Mixtures of less than 10 pesticides per water sample were most common for concurrent exposure. Exposure metrics based on the 5% fraction of a species sensitivity distribution performed best, closely followed by metrics based on the most sensitive species and Daphnia magna as benchmark. Considering only the compound with the highest toxicity and ignoring mixture toxicity was sufficient to estimate toxicity in predominantly agricultural regions with pesticide exposure. The multisubstance Potentially Affected Fraction (msPAF) that combines concentration and response addition was advantageous in the study where further organic toxicants occurred. We give recommendations on exposure metric selection depending on data availability and the involved compounds.
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Contaminantes Químicos del Agua/toxicidad , Animales , Monitoreo del AmbienteRESUMEN
Climate and land-use change drive a suite of stressors that shape ecosystems and interact to yield complex ecological responses (that is, additive, antagonistic and synergistic effects). We know little about the spatial scales relevant for the outcomes of such interactions and little about effect sizes. These knowledge gaps need to be filled to underpin future land management decisions or climate mitigation interventions for protecting and restoring freshwater ecosystems. This study combines data across scales from 33 mesocosm experiments with those from 14 river basins and 22 cross-basin studies in Europe, producing 174 combinations of paired-stressor effects on a biological response variable. Generalized linear models showed that only one of the two stressors had a significant effect in 39% of the analysed cases, 28% of the paired-stressor combinations resulted in additive effects and 33% resulted in interactive (antagonistic, synergistic, opposing or reversal) effects. For lakes, the frequencies of additive and interactive effects were similar for all spatial scales addressed, while for rivers these frequencies increased with scale. Nutrient enrichment was the overriding stressor for lakes, with effects generally exceeding those of secondary stressors. For rivers, the effects of nutrient enrichment were dependent on the specific stressor combination and biological response variable. These results vindicate the traditional focus of lake restoration and management on nutrient stress, while highlighting that river management requires more bespoke management solutions.
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Ecosistema , Agua Dulce , Biota , Europa (Continente) , RíosRESUMEN
The Water Framework Directive (WFD), which is the most comprehensive instrument of EU water policy, is more relevant than ever. Sixty percent of Europe's surface water bodies still fail to achieve good ecological status and a multitude of new stressors continue to emerge. A sustained and wholehearted water management effort is therefore of highest priority. Here, we present a new biological assessment approach specifically designed to safeguard sustainable water management under multiple stress. The framework contains three independent elements: 1) an ecological assessment system based on community abundance and composition to quantify ecological status; 2) a diagnostic tool to identify cause(s) of ecological degradation; 3) a management platform to guide the choice of relevant mitigation measures for improvement of the ecological status. The proposed framework is fully compliant with the WFD and currently applied in the assessment of aquatic plant communities in Danish streams. Importantly, the approach presented is not restricted to specific taxonomic groups or ecosystem types but is an example of how a simple approach can bring the conceptual idea of the WFD - that community characteristics in unimpacted, type-specific water bodies should be the backbone in ecological assessments - into practice.
RESUMEN
Freshwaters are among the most endangered ecosystems worldwide, due predominantly to excessive anthropogenic practices compromising the future provisioning of ecosystem services. Despite increased awareness of the role of multiple stressors in accounting for ecological degradation in mixed land-use stream systems, risk assessment approaches applicable in field settings are still required. This study provides a first indication for ecological consequences of the interaction of organic and inorganic chemical stressors, not typically evaluated together, which may provide a missing link enabling the reconnection of chemical and ecological findings. Specifically, impaired ecological conditions - represented by lower abundance of meiobenthic individuals - were observed in the hyporheic zone where a contaminant groundwater plume discharged to the stream. These zones were characterized by high xenobiotic organic concentrations, and strongly reduced groundwater (e.g. elevated dissolved iron and arsenic) linked to the dissolution of iron hydroxides (iron reduction) caused by the degradation of xenobiotic compounds in the plume. Further research is still needed to separate whether impact is driven by a combined effect of organic and inorganic stressors impacting the ecological communities, or whether the conditions - when present simultaneously - are responsible for enabling a specific chemical stressor's availability (e.g. trace metals), and thus toxicity, along the study stream. Regardless, these findings suggest that benthic meioinvertebrates are promising indicators for supporting biological assessments of stream systems to sufficiently represent impacts resulting from the co-occurrence of stressors in different stream compartments. Importantly, identification of the governing circumstances is crucial for revealing key patterns and impact drivers that may be needed in correctly prioritizing stressor impacts in these systems. This study further highlights the importance of stream-aquifer interfaces for investigating chemical stressor effects in multiple stressor systems. This will require holistic approaches for linking contaminant hydrogeology and eco(toxico)logy in order to positively influence the sustainable management of water resources globally.
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Headwater streams are important contributors to aquatic biodiversity and may counteract negative impacts of anthropogenic stress on downstream reaches. In Denmark, the first river basin management plan (RBMP) included streams of all size categories, most being <2.5m wide (headwater streams). Currently, however, it is intensely debated whether the small size and low slopes, typical of Danish streams, in combination with degraded habitat conditions obstruct their ability to fulfill the ecological quality objectives required by the EU Water Framework Directive (WFD). The purpose of this study was to provide an analytically based framework for guiding the selection of headwater streams for RBMP. Specifically, the following hypotheses were addressed: i) stream slope, width, planform, and general physical habitat quality can act as criteria for selecting streams for the next generation of RBMPs, and ii) probability-based thresholds for reaching good ecological status can be established for some or all of these criteria, thus creating a sound, scientifically based, and clear selection process. The hypotheses were tested using monitoring data on Danish streams from the period 2004-2015. Significant linear relationships were obtained between the ecological quality ratio assessed by applying the Danish Stream Fauna Index (DSFIEQR) and stream slope, width, sinuosity, and DHI. The obtained models were used to produce pressure-response curves describing the probability of achieving good ecological status along gradients in these parameters. Next, threshold values for slope, width, sinuosity, and DHI were identified for selected probabilities of achieving minimum good ecological status. The obtained results can support managers and policy makers in prioritizing headwater streams for the 3rd RBMP. The approach applied is broadly applicable and can, for instance, help prioritization of restoration and conservation efforts in different types of ecosystems where the biota can be significantly linked to separate and quantifiable environmental characteristics.
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A large part of the organic carbon in streams is transported by pulses of terrestrial dissolved organic carbon (tDOC) during hydrological events, which is more pronounced in agricultural catchments due to their hydrological flashiness. The majority of the literature considers stationary benthic biofilms and hyporheic biofilms to dominate uptake and processing of tDOC. Here, we argue for expanding this viewpoint to planktonic bacteria, which are transported downstream together with tDOC pulses, and thus perceive them as a less variable resource relative to stationary benthic bacteria. We show that pulse DOC can contribute significantly to the annual DOC export of streams and that planktonic bacteria take up considerable labile tDOC from such pulses in a short time frame, with the DOC uptake being as high as that of benthic biofilm bacteria. Furthermore, we show that planktonic bacteria efficiently take up labile tDOC which strongly increases planktonic bacterial production and abundance. We found that the response of planktonic bacteria to tDOC pulses was stronger in smaller streams than in larger streams, which may be related to bacterial metacommunity dynamics. Furthermore, the response of planktonic bacterial abundance was influenced by soluble reactive phosphorus concentration, pointing to phosphorus limitation. Our data suggest that planktonic bacteria can efficiently utilize tDOC pulses and likely determine tDOC fate during downstream transport, influencing aquatic food webs and related biochemical cycles.
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Determining whether the structural and functional stress responses of communities are similar across space and time is paramount for forecasting and extrapolating the consequences of anthropogenic pressures on ecosystems and their services. Stream ecosystems are under high anthropogenic pressure; however, studies have only examined the response of stream communities across large scales over multiple generations. We studied the responses of leaf-associated microbial communities in streams within three European biogeographical regions to chemical stress in a microcosm experiment with multiple cycles of fungicide pollution and resource colonisation. Fungal community composition and the ecosystem function leaf decomposition were measured as response variables. Microbial leaf decomposition showed similar recovery times under environmental levels of fungicide exposure across regions. Initially, the decomposition declined (between 19 and 53%) under fungicide stress and recovered to control levels during the third cycle of pollution and colonisation. Although community composition and its stress response varied between regions, this suggests similar functional community adaptation towards fungicide stress over time. Genetic, epigenetic and physiological adaptations, as well as species turnover, may have contributed to community adaptation but further studies are required to determine if and to which extent these mechanisms are operating. Overall, our findings provide the first evidence of a similar functional response of microbial leaf decomposition to chemical stress across space and time.
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Fungicidas Industriales/toxicidad , Microbiota/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidad , Biomasa , Ecosistema , Geografía , Hojas de la Planta/microbiología , Ríos/químicaRESUMEN
Worldwide, lowland stream ecosystems are exposed to multiple anthropogenic stress due to the combination of water scarcity, eutrophication, and fine sedimentation. The understanding of the effects of such multiple stress on stream benthic macroinvertebrates has been growing in recent years. However, the interdependence of multiple stress and stream habitat characteristics has received little attention, although single stressor studies indicate that habitat characteristics may be decisive in shaping the macroinvertebrate response. We conducted an experiment in large outdoor flumes to assess the effects of low flow, fine sedimentation, and nutrient enrichment on the structure of the benthic macroinvertebrate community in riffle and run habitats of lowland streams. For most taxa, we found a negative effect of low flow on macroinvertebrate abundance in the riffle habitat, an effect which was mitigated by fine sedimentation for overall community composition and the dominant shredder species (Gammarus pulex) and by nutrient enrichment for the dominant grazer species (Baetis rhodani). In contrast, fine sediment in combination with low flow rapidly affected macroinvertebrate composition in the run habitat, with decreasing abundances of many species. We conclude that the effects of typical multiple stressor scenarios on lowland stream benthic macroinvertebrates are highly dependent on habitat conditions and that high habitat diversity needs to be given priority by stream managers to maximize the resilience of stream macroinvertebrate communities to multiple stress.
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Ecosistema , Monitoreo del Ambiente , Invertebrados , Calidad del Agua , Animales , Sedimentos Geológicos , Ríos , Estrés FisiológicoRESUMEN
We couple current findings of pesticides in surface and groundwater to the history of pesticide usage, focusing on the potential contribution of legacy pesticides to the predicted ecotoxicological impact on benthic macroinvertebrates in headwater streams. Results suggest that groundwater, in addition to precipitation and surface runoff, is an important source of pesticides (particularly legacy herbicides) entering surface water. In addition to current-use active ingredients, legacy pesticides, metabolites and impurities are important for explaining the estimated total toxicity attributable to pesticides. Sediment-bound insecticides were identified as the primary source for predicted ecotoxicity. Our results support recent studies indicating that highly sorbing chemicals contribute and even drive impacts on aquatic ecosystems. They further indicate that groundwater contaminated by legacy and contemporary pesticides may impact adjoining streams. Stream observations of soluble and sediment-bound pesticides are valuable for understanding the long-term fate of pesticides in aquifers, and should be included in stream monitoring programs.
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Monitoreo del Ambiente , Plaguicidas/análisis , Ríos/química , Contaminantes Químicos del Agua/análisis , Contaminación Química del Agua/estadística & datos numéricos , Ecosistema , Ecotoxicología , Herbicidas , InsecticidasRESUMEN
Agricultural insecticides constitute a major driver of animal biodiversity loss in freshwater ecosystems. However, the global extent of their effects and the spatial extent of exposure remain largely unknown. We applied a spatially explicit model to estimate the potential for agricultural insecticide runoff into streams. Water bodies within 40% of the global land surface were at risk of insecticide runoff. We separated the influence of natural factors and variables under human control determining insecticide runoff. In the northern hemisphere, insecticide runoff presented a latitudinal gradient mainly driven by insecticide application rate; in the southern hemisphere, a combination of daily rainfall intensity, terrain slope, agricultural intensity and insecticide application rate determined the process. The model predicted the upper limit of observed insecticide exposure measured in water bodies (n = 82) in five different countries reasonably well. The study provides a global map of hotspots for insecticide contamination guiding future freshwater management and conservation efforts.
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Agricultura/estadística & datos numéricos , Monitoreo del Ambiente , Agua Dulce/química , Insecticidas/análisis , Modelos Químicos , Contaminantes Químicos del Agua/análisis , Ecosistema , RíosRESUMEN
We revealed a history of legacy pesticides in water and sediment samples from 19 small streams across an agricultural landscape. Dominant legacy compounds included organochlorine pesticides, such as DDT and lindane, the organophosphate chlorpyrifos and triazine herbicides such as terbutylazine and simazine which have long been banned in the EU. The highest concentrations of legacy pesticides were found in streams draining catchments with a large proportion of arable farmland suggesting that they originated from past agricultural applications. The sum of toxic units (SumTUD.magna) based on storm water samples from agriculturally impacted streams was significantly higher when legacy pesticides were included compared to when they were omitted. Legacy pesticides did not significantly change the predicted toxicity of water samples to algae or fish. However, pesticide concentrations in bed sediment and suspended sediment samples exceeded safety thresholds in 50% of the samples and the average contribution of legacy pesticides to the SumTUC.riparius was >90%. Our results suggest that legacy pesticides can be highly significant contributors to the current toxic exposure of stream biota, especially macroinvertebrate communities, and that those communities were primarily exposed to legacy pesticides via the sediment. Additionally, our results suggest that neglecting legacy pesticides in the risk assessment of pesticides in streams may severely underestimate the risk of ecological effects.
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Monitoreo del Ambiente/métodos , Agua Dulce/análisis , Plaguicidas/análisis , Contaminantes Químicos del Agua/análisis , Contaminación Química del Agua/análisis , Medición de RiesgoRESUMEN
Mitigation activities to improve water quality and quantity in streams as well as stream management and restoration efforts are conducted in the European Union aiming to improve the chemical, physical and ecological status of streams. Headwater streams are often characterised by impairment of hydromorphological, chemical, and ecological conditions due to multiple anthropogenic impacts. However, they are generally disregarded as water bodies for mitigation activities in the European Water Framework Directive despite their importance for supporting a higher ecological quality in higher order streams. We studied 11 headwater streams in the Hove catchment in the Copenhagen region. All sites had substantial physical habitat and water quality impairments due to anthropogenic influence (intensive agriculture, urban settlements, contaminated sites and low base-flow due to water abstraction activities in the catchment). We aimed to identify the dominating anthropogenic stressors at the catchment scale causing ecological impairment of benthic macroinvertebrate communities and provide a rank-order of importance that could help in prioritising mitigation activities. We identified numerous chemical and hydromorphological impacts of which several were probably causing major ecological impairments, but we were unable to provide a robust rank-ordering of importance suggesting that targeted mitigation efforts on single anthropogenic stressors in the catchment are unlikely to have substantial effects on the ecological quality in these streams. The SPEcies At Risk (SPEAR) index explained most of the variability in the macroinvertebrate community structure, and notably, SPEAR index scores were often very low (<10% SPEAR abundance). An extensive re-sampling of a subset of the streams provided evidence that especially insecticides were probably essential contributors to the overall ecological impairment of these streams. Our results suggest that headwater streams should be considered in future management and mitigation plans. Catchment-based management is necessary because several anthropogenic stressors exceeded problematic thresholds, suggesting that more holistic approaches should be preferred.
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Agua Dulce/análisis , Invertebrados/efectos de los fármacos , Contaminantes Químicos del Agua/análisis , Calidad del Agua/normas , Animales , Dinamarca , Monitoreo del Ambiente , Agua Dulce/química , Sedimentos Geológicos/análisis , Invertebrados/crecimiento & desarrollo , Urbanización , Contaminantes Químicos del Agua/toxicidadRESUMEN
The release of chemicals such as chlorinated solvents, pesticides and other xenobiotic organic compounds to streams, either from contaminated sites, accidental or direct application/release, is a significant threat to water resources. In this paper, different methods for evaluating the impacts of chemical stressors on stream ecosystems are evaluated for a stream in Denmark where the effects of major physical habitat degradation can be disregarded. The methods are: (i) the Danish Stream Fauna Index, (ii) Toxic Units (TU), (iii) SPEAR indices, (iv) Hazard Quotient (HQ) index and (v) AQUATOX, an ecological model. The results showed that the hydromorphology, nutrients, biological oxygen demand and contaminants (pesticides and trichloroethylene from a contaminated site) originating from groundwater do not affect the good ecological status in the stream. In contrast, the evaluation by the novel SPEAR(pesticides) index and TU indicated that the site is far from obtaining good ecological status - a direct contradiction to the ecological index currently in use in Denmark today - most likely due to stream sediment-bound pesticides arising from the spring spraying season. In order to generalise the findings of this case study, the HQ index and AQUATOX were extended for additional compounds, not only partly to identify potential compounds of concern, but also to determine thresholds where ecological impacts could be expected to occur. The results demonstrate that some commonly used methods for the assessment of ecological impact are not sufficient for capturing - and ideally separating - the effects of all anthropogenic stressors affecting ecosystems. Predictive modelling techniques can be especially useful in supporting early decisions on prioritising hot spots, serving to identify knowledge gaps and thereby direct future data collection. This case study presents a strong argument for combining bioassessment and modelling techniques to multi-stressor field sites, especially before cost-intensive studies are conducted.