RESUMEN
The paper deals with the real application of a strategy, based on process control automation and remote on-line supervision, for the wastewater treatment in a piedmont. Seven existing small wastewater treatment plants were selected to be upgraded and to be included into a network remotely supervised. A consolidated bending point based alternating process was applied for the biological treatment. Further, the potentialities of the process control automation were enhanced by the appropriate design of the whole plant. The examination of a case study included into the network shows the real stable high performances of the plant in terms of total nitrogen removal. Moreover, the power requirements are significantly reduced according to a correct energy policy.
Asunto(s)
Programas Informáticos , Eliminación de Residuos Líquidos/métodos , Purificación del Agua/métodos , Automatización , Monitoreo del Ambiente/métodosRESUMEN
Afibrinogenemia represents the rarest form of fibrinogen deficiency. Causative missense mutations occur rarely and may improve the understanding of fibrinogen structure and function. PATIENTS AND METHODS: The propositus was a 26-year-old Argentinian with afibrinogenemia. FGA, FGB and FGG exons and flanking regions were screened by sequencing and the mutant protein was analyzed in silico. RESULTS: A novel missense mutation in the FGB gene (Bbeta Gly272Arg, p.Gly302Arg) was identified. In silico analysis revealed its location in a highly conserved region, which preserves the core fold of the C-terminal beta-chain and is important for proper secretion. A substitution by a positively charged large Arg residue in this area would most likely disturb the core fold by additional interactions with adjacent residues (p.Asp291, p.Asp297, p.Asp311), or by forming of non-native interactions with other proteins, thereby hindering the action of molecular chaperones. Both alternatives would disturb the regular secretion of the beta-chain. CONCLUSIONS: The novel missense mutation in the FGB gene causes afibrinogenemia most probably by affecting the secretion of the fibrinogen beta-chain.
Asunto(s)
Afibrinogenemia/diagnóstico , Afibrinogenemia/genética , Codón sin Sentido/genética , Fibrinógeno/genética , Hemorragia/diagnóstico , Hemorragia/genética , Adulto , Afibrinogenemia/complicaciones , Secuencia de Bases , Diagnóstico Diferencial , Fibrinógeno/química , Fibrinógeno/ultraestructura , Predisposición Genética a la Enfermedad/genética , Pruebas Genéticas/métodos , Hemorragia/complicaciones , Humanos , Masculino , Datos de Secuencia Molecular , Enfermedades Raras/diagnóstico , Enfermedades Raras/genética , Relación Estructura-ActividadRESUMEN
Prevalence of alpha gene triplication or deletion in beta-thalassemia carriers was studied in 109 unrelated individuals in Rosario, Argentina. In different populations -alpha(3.7) allele presents a higher prevalence than alphaalphaalpha(anti3.7); thus, alpha-thalassemia associated with beta-thalassemia is more frequently observed. Nevertheless, this event was detected in only one case (0.9%), while the association with alpha triplication was present in two subjects (1.8%).