RESUMEN
Prostaglandin are natural fatty acid derivatives with diverse physiological effects, including immune function and the control of cell growth. While the action of prostaglandins in the induction of stress proteins in vertebrate cells in well documented, their functions in invertebrate cells have been poorly investigated. The purpose of the present study was to investigate the effect of prostaglandin A1 (PGA1; 0.25, 1.25 and 12.5 mug/ml) on protein synthesis during the growth of Aedes albopictus cells. We found that PGA1 stimulates the synthesis of several polypeptides with molecular masses of 87,80,70,57,29,27 and 23 kDa in Aedes albopictus cells. When the protein induced by PGA1 and those induced by heat treatment were compared by polyacrylamide gel electrophoresis, PGA1 was found to induce the stress proteins. The HSP70 family and the low-molecular weight polypeptides (29 and 27 kDa, respectively) were induced by PGA1 in the lag phase. We lso observed that PGA1 is able to induce a 23-kDa polypeptide independently of the growth phase of the cell.
Asunto(s)
Animales , Aedes/citología , Proteínas de Choque Térmico/efectos de los fármacos , Prostaglandinas A/farmacología , División Celular/efectos de los fármacosRESUMEN
The effect of prostaglandins (PGA1 and PGB2) on the replication of Mayaro virus was studied in Vero cells. PGA1 and PGB2 antiviral activity was found to be dose-dependent. However, while 10 µg/ml PGB2 inhibited virus yield by 60 percent, at the same dose PGA1 suppressed virus replication by more than 90 percent. SDS-PAGE analysis of [35S]-methionine-labelled proteins showed that PGA1 did not alter cellular protein synthesis. In infected cells, PGA1 slightly inhibited the synthesis of protein C, while drastically inhibiting the synthesis of glycoproteins E1 and E2
Asunto(s)
Animales , Alphavirus/fisiología , Prostaglandinas A/farmacología , Prostaglandinas B/farmacología , Células Vero/efectos de los fármacos , Replicación Viral/efectos de los fármacos , Infecciones por Alphavirus/tratamiento farmacológico , Alphavirus/efectos de los fármacos , Alphavirus/crecimiento & desarrollo , Glicoproteínas/biosíntesis , Metionina/análisis , Prostaglandinas A/metabolismo , Prostaglandinas A/uso terapéutico , Prostaglandinas B/metabolismo , Prostaglandinas B/uso terapéutico , Proteína C/biosíntesisRESUMEN
Mayaro virus was grown in BHK-21 cells and purified by centrifugation in a potassium-tartrate gradient (5-50%). The electron microscopy analyses of the purified virus showed an homogeneous population of enveloped particles with 69 ñ 2.3 nm in diameter. Three structural virus proteins were identified and designated pl, p2 and p3. Their average molecular weight were p1, 54 KDa; p2, 50 KDa and p3, 34 KDa. In Mayaro virus infected. Aedes albopictus cells and in BHK-21 infected cells we detected six viral proteins, in wich three of them are the structural virus proteins and the other three were products from processing of precursors of viral proteins, whose molecular weights are 62 KDa, 64 KDa and 110 KDa. The 34 KDa protein was the first viral protein sinthesized at 5 hours post-infection in both cell lines studied
Asunto(s)
Arbovirus/ultraestructura , Proteínas ViralesRESUMEN
Mayaro virus grown in interferon treated infected cells has been characterized with regard to its ability to replicate in vertebrate (TC7) and invertebrate (Aedes albopictus) cells. Virus purified from interferon treated TC7 cells adsorbs and penetrates to the same extent as the control virus. During infection, these virus particles caused inhibition of host protein synthesis and synthesized the same spectrum of viral proteins as normal virus. This population however, was apparently more sensitive to interferon treatment. Electron microscopy of TC7 cells showed the presence of numerous aberrant virus particles budding from the plasma membrane.
Asunto(s)
Animales , Alphavirus , Interferón-alfa , Replicación Viral/efectos de los fármacos , Aedes , Alphavirus , Electroforesis en Gel de Poliacrilamida , Interferón-alfa , Proteínas Virales/biosíntesisRESUMEN
Prostaglandin A1 (PGA1) reduced Mayaro virus replication in Aedes albopictus (mosquito) cells in culture. The highest nontoxic dose of PGA1, 7.5µM, decreased virus production by 90 percent. In Mayaro virus-=infected cells, PGA1 inhibited virus-specific protein synthesis. However, in mock-infected cells the presence of PGA, stimulated the synthesis of several proteins with molecular masses of 70, 57 and 23 kDa, respectively. The data obtained from this study show that PGA1 plays a role in the metabolic regulation of Aedes albopictus cells, blocking the synthesis of Mayaro virus and inducing the synthesis of cellular polypeptides
Asunto(s)
Animales , Aedes/virología , Alphavirus/fisiología , Péptidos/biosíntesis , Prostaglandinas A/farmacología , Proteínas Virales/biosíntesis , Replicación ViralRESUMEN
Isoprinosine (IPS) is a synthetic drug whose antiviral effect on rotavirus replication in vitro has been characterized in terms of the decrease in metachromasia after acridine orange staining. The present study describes the effect of IPS on the synthesis of viral RNA in vitro. MA-104 cell cultures infected with simian rotavirus strain SA-11 were incubated with zero, 250, 500 and 1,000 microg/ml IPS and 22, 24, 48, 52, 72 and 76 h after infection the cultures were submitted to a 1-h starvation period, followed by a 2-h pulse with 10 microCi/ml of [3H]-uridine. The homogenates of virus-infected cultures treated or not with IPS were submitted to phenol/chloroform extraction followed by polyacrylamide gel electrophoresis. The amount of radioactivity in viral RNA eluted from the gel strips was determined. Inhibition of viral RNA synthesis was highest at the IPS concentration of 1,000 microg/ml at 72 h after infection, corresponding to 78 percent inhibition. Although the results obtained in vitro suggest that IPS may be useful for the treatment of rotavirus infection, an in vivo demonstration of its efficacy is needed.
Asunto(s)
Técnicas In Vitro , Inosina Pranobex/farmacología , Rotavirus/efectos de los fármacos , Replicación Viral , Rotavirus/crecimiento & desarrolloRESUMEN
the multiplication of Mayaro virus in Aedes albopictus cells was drastically inhibited after incubation at 37-C. The effect of short-term exposure of infected cells to high temperatures (heat shock) produced a preferential translation of the heat shock messengers when compared to the viral mRNAs. When cells were shifted back to 28-C (the optimum growth temperature for Aedes albopictus cells), preferential translation of viral mRNA occurred. Although the infected cells were programmed for preferential translation of viral messengers, the therminal treatment was able to shif the translational machinery towards synthesis of heat shock proteins
Asunto(s)
Animales , Aedes/microbiología , Alphavirus/fisiología , Calor , Alphavirus/genética , Células Clonales/microbiología , Regulación de la Expresión Génica , Biosíntesis de Proteínas , ARN Viral , Replicación ViralRESUMEN
L (+) lactate dehydrogenase (LDH) activity from cultured cells of Aedes albopictus was studies as a kinetic model of carbohydrate metabolism. Enzyme kinetics were studied in the forward (lactate as substrte) and reverse (pyruvate as substrate) reactions and the apparent Km values were obtained showing LDH higher affinity for pyruvate. The Hill coefficient values for each substrate were similar and indicate the existence of only one binding site on the enzyme. Isozyme analysis on cellulose-acetate electrophoresis presented a singe band of LDH which preumably is of the LDH-5 type. The results obtained contribute to the assumption that Aedes albopictus cells have a predominance of anaerobic metabolism