Microfluidic Breadboard Approach to Capillary Electrophoresis.

A breadboard approach for electrophoretic separations with contactless conductivity detection is presented. This is based on miniature off-the-shelf components such as syringe pumps, valves, and pressure controllers which could be set up in a very compact overall arrangement. It has a high flexibility for different tasks at hand, and the common operations of hydrodynamic injection and capillary flushing are automated. For demonstration of the versatility of the proposition, several very diverse configurations and modes of electrophoresis were successfully implemented, namely, standard capillary zone electrophoresis, pressure assisted zone electrophoresis, the simultaneous separation of cations and anions by dual-capillary zone electrophoresis, the separation of cationic amino acids by isotachophoresis, as well as the separation of small carboxylic acids by gradient elution moving boundary electrophoresis. The system also allows fast separations, as demonstrated by the analysis of six inorganic cations within 35 s. The approach addresses respective limitations of either conventional capillary electrophoresis instruments as well as electrophoretic lab-on-chip devices, while maintaining a performance in terms of detection limits and reproducibility comparable to standard instrumentation.

The effect of two alternative arm supports on shoulder and upper back muscle loading during pipetting.

OBJECTIVE: Pipetting involves static upper arm positions with the pipette held away from the body for sustained periods of time, putting increased musculoskeletal load on the shoulder and upper back. This study explores the effect of using two alternative arm supports while pipetting on muscle loading in the shoulder/neck region. PARTICIPANTS: 15 experienced pipette users participated in this study. METHODS: In a repeated-measures design, participants performed simulated pipetting in a laboratory setting under three arm support conditions: (1) a gel pad on the work surface, (2) a freely-moving counter-balanced forearm support, and (3) no support (control). Surface electromyography (EMG) of the anterior deltoid and upper trapezius muscles were recorded, as well as productivity and subjective usability. RESULTS: Both arm support conditions resulted in significantly lower mean muscle activity of the anterior deltoid and upper trapezius muscles (p<0.001) and significantly higher subjective comfort ratings (p<0.001) compared to the control condition. The freely moving forearm support resulted in significantly lower peak muscle activity in the anterior deltoid compared to the control condition (p<0.001). Productivity was not affected by the arm supports. These findings suggest that arm support may be beneficial in reducing muscle loading and improving comfort in the shoulder and upper back during pipetting. Future studies are needed to measure the impact of these arm supports in the workplace.