Insulin and SGK1 reduce the function of Na+/monocarboxylate transporter 1 (SMCT1/SLC5A8).

SMCTs move several important fuel molecules that are involved in lipid, carbohydrate, and amino acid metabolism, but their regulation has been poorly studied. Insulin controls the translocation of several solutes that are involved in energetic cellular metabolism, including glucose. We studied the effect of insulin on the function of human SMCT1 expressed in Xenopus oocytes. The addition of insulin reduced α-keto-isocaproate (KIC)-dependent 22Na+ uptake by 29%. Consistent with this result, the coinjection of SMCT1 with SGK1 cRNA decreased the KIC-dependent 22Na+ uptake by 34%. The reduction of SMCT1 activity by SGK1 depends on its kinase activity, and it was observed that the coinjection of SMCT1 with S442D-SGK1 (a constitutively active mutant) decreased the KIC-dependent 22Na+ uptake by 50%. In contrast, an SMCT1 coinjection with K127M-SGK1 (an inactive mutant) had no effect on the KIC-dependent Na+ uptake. The decreasing SMCT1 function by insulin or SGK1 was corroborated by measuring [1-14C]acetate uptake and the electric currents of SMCT1-injected oocytes. Previously, we found that SMCT2/Slc5a12-mRNA, but not SMCT1/Slc5a8-mRNA, is present in zebrafish pancreas (by in situ hybridization); however, SLC5a8 gene silencing was associated with the development of human pancreatic cancer. We confirmed that the mRNA and protein of both transporters were present in rat pancreas using RT-PCR with specific primers, Western blot analysis, and immunohistochemistry. Additionally, significant propionate-dependent 22Na+ uptake occurred in pancreatic islets and was reduced by insulin treatment. Our data indicate that human SMCT1 is regulated by insulin and SGK1 and that both SMCTs are present in the mammalian pancreas.

Compra y consumo de alimentos ante el confinamiento por SARSCoV-2 en un grupo de pacientes pertenecientes a una Clínica de Salud Incluyente en la Ciudad de México / Purchase and food consumption among a group of patients in an Inclusive Health Clinic due to confinement of SARS-CoV-2

Fundamentos: Se tiene poca información sobre compra y consumo de alimentos ante el confinamiento por SARSCoV-2 en pacientes socioeconómicamente vulnerables. El objetivo fue evaluar la compra y consumo de alimentosante confinamiento por SARS-CoV-2 de pacientes con sobrepeso u obesidad pertenecientes a una Clínica de Salud Incluyente en la Ciudad de México. Métodos: Estudio observacional, transversal analítico, con pacientes de ambos sexos, entre 30 y 70 años, con sobrepeso u obesidad, sin ansiedad y depresión severa. Vía telefónica, se preguntó por la compra y el consumo de alimentos, percepción de cambios de peso corporal y tiempo para preparar alimentos. Se aplicó estadística descriptiva, pruebas de chi-cuadrado y razón de momios. El nivel de significancia fue de 0,05. Resultados: Muestra de 30 pacientes, 50% sexo femenino, con una edad promedio 57,2 ± 5,9 años. La más alta percepción de aumento de precio y escasez fue en los grupos de frutas, verduras y alimentos de origen animal. Consumen dieta completa en frutas y verduras: 33,3%; alimentos de origen animal: 56% y cereales: 100% de los pacientes. Se determinó que el 6,7% tenía dieta inocua y el 43,3% variada. La percepción de aumento de precio de alimentos de origen animal y leguminosas se relacionó con la de aumento de peso corporal (OR:21; IC95% 1,833- 240,52 y OR:35; IC95% 1,780-693,41). Conclusiones: Durante confinamiento, la percepción de aumento de precio y escasez de frutas, verduras y alimentos de origen animal puede explicar su bajo consumo. Las recomendaciones de nutrición deberán facilitar consumo de frutas, verduras, leguminosas y alimentos de origen animal entre pacientes socioeconómicamente vulnerables. (AU)

Background: There is little information on the purchase and consumption of food in the face of confinement in underserved patients. Objective: To evaluate purchase and consumption of food during the confinement due to SARS-CoV-2 among overweight or obese patients in an Inclusive Health Clinic in Mexico City. Methods: Observational, cross-sectional analytical study, with patients of both sexes, between 30 and 70 years old, with overweight or obesity, without severe anxiety and depression. Through telephone calls, purchase and consumption of food, changes in body weight perception and in the time to prepare food were asked. Descriptive statistics, chi square and odd ratios were applied. The significance level 0.05. Results: The sample of 30 patients, 50% women, average age 57.2 (+5.9) years. The highest proportion of perception of price increase and scarcity was in the fruits and vegetables and animal products. With complete diet in fruits and vegetables: 33.3%; animal products: 56% and cereals: 100%. From all, 6.7% innocuous diet and 43% varied diet. The perception of increase price in animal products and legumes is related to perceiving an increase in body weight (OR: 21; 95% CI: 1.833-240.52 and OR: 35; 95% CI 1.780-693.41). Conclusions: During confinement, the perception of price increase and scarcity in fruits, vegetables and animal products explain the low amount of consumption. Conclusion: Based on our results, the nutrition recommendations need to facilitate consumption of fruits, vegetables, legumes and animal products among uderserved patients. (AU)