RESUMEN
Todays, with the industrialization of human societies, pollution of aquatic ecosystems with plastics derivatives are a serious concern, affecting the life of their organisms. The present study was conducted to investigate the size effects of micro-plastic, polystyrene on some physiological lesions of the goldfish, Carassius auratus. Fish were exposed to two sizes (0.25 and 8 µm) polystyrene at different environmentally relevant concentrations. The exposure trial was done in two steps. First, fish exposed to a stable concentration of 300 mg/L polystyrene for 168 h. Gill, intestine, and liver tissues were sampled every 24 h to investigate the accumulation of polystyrene. Then, fish were exposed in three replicates to 0 (control), 0.05, 0.5, and 5 mg/L polystyrene in two sizes of 0.25 and 8 µm for 28 days. After the exposure period, gill, liver, and intestine tissues were sampled for histological study, also, serum samples were collected for biochemical assays. Fluorescent microscope observations confirmed the accumulation of polystyrene in tissue samples with time. In addition, histological lesions were found in the liver, intestine, and gill of the exposed fish. The severity of lesions showed a size and dose-dependent pattern. Polystyrene induced the antioxidant system of exposed fish through elevating the levels of SOD and CAT activity and significant difference in expression of antioxidant related genes (CAT, SOD and HSP70). In conclusion, the results of the present study confirmed the toxic effects of microplastic, polystyrene on goldfish.