RESUMEN
Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 and DEAE S2) by enzyme-linked immunosorbent assay (ELISA, n = 123) and immunoblotting (IB, n = 22) to detect human NC in serum. Diagnostic parameters were established by ROC and TG ROC curves for ELISA tests. IB was qualitatively analyzed. S and DEAE S1 presented sensitivity of 87. 5% and DEAE S2 90%. The best specificity was observed for DEAE S2 (90.4%). In IB, using DEAE S2 samples from NC patients presented bands of 20-25, 43-45, 55-50, 60-66, 82, 89, and 140 kDa. The great diagnostic parameters reached by DEAE S2 suggest the potential applicability of this fraction in NC immunodiagnosis.
Asunto(s)
Anticuerpos Antihelmínticos/sangre , Antígenos Helmínticos/aislamiento & purificación , Etanolaminas/química , Inmunoglobulina G/sangre , Neurocisticercosis/diagnóstico , Taenia solium/inmunología , Animales , Antígenos Helmínticos/inmunología , Fraccionamiento Químico , Cromatografía por Intercambio Iónico , Ensayo de Inmunoadsorción Enzimática , Humanos , Immunoblotting , Neurocisticercosis/sangre , Neurocisticercosis/inmunología , Sensibilidad y Especificidad , Pruebas Serológicas , Porcinos , Taenia solium/aislamiento & purificaciónRESUMEN
We report the detection of IgG, IgG1, IgG4 and IgE anti-Strongyloides stercoralis as complementary tool for screening in patients with diabetes in hyperendemic areas for strongyloidiasis. A panel of 119 serum samples were analyzed: 76 from patients with DM2 and 43 patients with other endocrine diseases and a positive correlation for total IgG levels with IgG4 (rs = 0.559; P = 0.024; n = 16) and IgG and IgE (rs = 0.585; P < 0.0001; n = 76) was found in the diabetes group.